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1043 matches found for gene_expression

Higher sensitivity of qPCR reactions with BRAND 384-well PCR plates

In many laboratories real-time quantitative PCR (RT-qPCR) has become a standard technique to correlate phenotypic observations not only with altered protein expression data but also with quantitative changes on a transcriptional level. The quality of results obtained e.g. by RT-qPCR depends among ot...

Molecular Biology Handbook

Molecular Biology is the science that aims to understand biological activity at the molecular level. These biological activities usually involve the plant or animal cell, and the nucleic acids and proteins that are at work within the cell. Molecular Biologists use some standard techniques that have ...

Technical Articles on DNA and RNA Purification

• GenElute™ HP Plasmid Kits • GenElute™ Bacterial Genomic DNA Miniprep Kit • GenElute™ Blood Genomic DNA Miniprep Kit • GenElute™ Mammalian Genomic DNA Miniprep Kit • GenElute™ Plant Genomic DNA Miniprep Kit • GenElute™ Gel Extract Kit • GenElute™ PCR Clean-Up Kit • GenElute™ Mammalian Total RNA Kit...

CompoZr® Zinc Finger Nucleases FP-ZFN-IDLV

CompoZr Zinc Finger Nucleases (ZFNs) are a class of engineered DNA-binding proteins that facilitate highly specific targeted editing of any genome by creating double-stranded breaks in DNA at user specified locations. You can use ZFNs to create your choice of stable genetically engineered cell lines...

ABC Transporters and Cancer Drug Resistance

• • Download PDF • Role of ABC Transporters and Multi-Drug Resistance Reversal Using RNA Interference (RNAi)* Chemotherapy is the treatment of choice against many types of cancer. However, over time chemotherapeutic drugs can become less effective due to the development of resistance that involves a...

shRNA Frequently Asked Questions for Experimental Design

What controls do you recommend? Why doesn't the non-target control vector affect gene expression? How many cells can I infect with the amount of lentivirus provided? What is the method you recommend for screening the stable clones and is there a pair of primers that can be used for screening them by...

CHOZN® Platform Technical Bulletin

• • Download PDF • Product Description/Overview Precautions and Disclaimer Storage and Stability Background Part I: Medium Preparation Part II: Stock culture initiation/thawing and subculturing Part III: Stock culture maintenance Part IV: Cell banking Part V: Transfection Part VI: Stable pool select...

Comparing Quantitative Viability Bioassays: An Evaluation of MTT, alamarBlue™, and Guava® ViaCount® Methods

Krista McCutcheon and David Fei, Department of Analytical Sciences, Genentech Inc., South San Francisco, CA 94080-4990 Viability assays are crucial tools in the development of many drugs, particularly of cytotoxic agents. To best characterize potential compounds, viability assays must ideally be abl...

GenElute HP Endotoxin-Free Maxiprep Kit Article

By Dave Cutter and Goodarz Goodarzi Sigma-Aldrich Corporation, St. Louis, MO, USA View pdf version (209 KB) On this page: • Introduction • Materials and Methods • Results and Discussion • Conclusions • Acknowledgements • References • Ordering Information Introduction Plasmid DNA purification kits th...

meiosis/spermiogenesis associated 1
Synonyms SPATA39, bA2K17.3
Species Human (644890) , Species Mouse (104362) , Species Rat (679834) , Species Zebrafish (541343) , Species domestic cat (101089778) , Species cow (617353) , Species dog (100684014) , Species chicken (769589) , Species domestic guinea pig (100728794) , Species naked mole-rat (101699891) , Species sheep (101109199)

Alkaline Phosphatase Detection Kit, Fluorescence

Catalog Number: APF Storage Temperature –20 °C Product Description Components Equipment and reagents required but not provided Precautions and Disclaimer Storage/Stability Procedure Troubleshooting Materials The gene for alkaline phosphatase (AP) commonly serves as a reporter gene and is used to det...

Enhancing Lentiviral Transduction Efficiency

• • Download PDF • Research and Development, Biotechnology Division, Sigma-Aldrich An experiment to directly compare three methods of lentiviral transduction of Jurkat cells was conducted in order to determine the method that yields the greatest transduction efficiency. Spinoculation was carried out...

Tips for Optimizing Immunofluorescence Protocols to Get the Best Image

Related Links • Antibody Explorer: Search primary and secondary antibodies • Prestige Antibodies • Antibody Resource Center Uses for immunofluorescence (IF)—where an antibody is conjugated to a molecule that fluoresces when excited by lasers— include protein localization, confirmation of post-transl...

Dandelion (Taraxacum officinale) | Plant Profiler

Synonyms / Common Names / Related Terms Artemetin, Asteraceae (family), beta-carotene, blowball, caffeic acid, cankerwort, clock flower, common dandelion, Cichoroideae (sub-family), Compositae (family), dandelion herb, dandelion T-1 extract, dent de lion, diente de lion, dudhal, dumble-dor, epoxide,...

microRNA (miRNA) Stabilization in RNAstable™

Introduction miRNAs play an important role in gene regulation. Genes encoding miRNAs are transcribed and then processed into mature single-stranded stem-loop structures that then bind to complementary mRNA transcripts to down-regulate gene expression. Dysregulation of miRNA expression and function h...

Bacterial Transformation

Related Articles • Transforming E. coli with Engineered Plasmid • Transformation Protocols • Introduction to Blue-White Screening • Preparation of Competent Cells and Transformation with pGEX DNA Bacterial transformation is a process of horizontal gene transfer by which some bacteria take up foreign...

Frequently Asked Questions for General RNAi Use

What is RNAi? Why do I want to do RNAi? What is a gene accession number? What are RefSeq Numbers? Where do I find RefSeq Numbers? I cannot find my target gene in your search engine. Do you have it? Why can’t I find my gene using my gene symbol? For what species do you have predesigned siRNA or shRNA...

Retinoic Acid and Gene Expression

• • Download PDF • All-trans retinoic acid (RA, ATRA) is a pleiotropic activation factor that regulates genes associated with normal vertebrate cellular processes such as cell differentiation, cell proliferation, apoptosis, and embryonic development. Retinoic acid and the receptor proteins for which...

Adult Human Stem Cells in Regenerative Medicine

BioFiles 2008, 3.11, 6. Dr. Gerhard Bauer Laboratory Director GMP Facility Adjunct Assistant Professor Stem Cell Program School of Medicine, University of California Davis Stem cell therapies offer hope to thousands of people suffering from diseases or injuries that destroy or damage vital cells and...

Glutamate Receptors (G Protein Family)

The existence of G protein-coupled glutamate receptors (also called "€œmetabotropic" glutamate or mGlu's), belonging to the seven transmembrane spanning superfamily of receptors, was shown definitively with the cloning of the first member in 1991. Since then, eight receptors of this class have been ...

shRNA Frequently Asked Questions for TRC Design and pLKO.1 Vector Use

Why is the U6 promoter used? What restriction sites were used for cloning the shRNA sequences? Does pLKO.1 contain WPRE? What restriction enzymes are recommended for diagnostic digests of the clone DNA? How large of an insert can pLKO.1 handle? If I order TRC1.5 clones and controls, will they work w...

Chromatin Immunoprecipitation (ChIP)

Chromatin Immunoprecipitation (ChIP) assays are used to evaluate transcription factor-DNA interactions and they are critical for advancing our understanding of gene expression regulation and epigenetic modifications. ChIP can detect and relatively quantify specific protein-DNA and protein-protein in...

Rooibos (Aspalathus linearis) | Plant Profiler

Synonyms / Common Names / Related Terms Aspalathin, Aspalathus acuminatus, Aspalathus contaminata, Borbornia pinfolia, chrysoeryol, Fabaceae/Leguminosae (family), hyperoside, isoorientin, isoquercitrin, isovitexin, Kaffree tea, long life tea, luteolin, orientin, Psoralea linearis, quercetin, (R)-eri...

Introduction to Cell Transfection

Related Content • X-tremeGENE™ Transfection Reagents • Roche Biochemical Reagents • Cloning & Expression Portfolio • X-tremeGENE™ Transfection Database • X-tremeGENE™ General Recommendations Related Videos • How Transfection Works • Transfection Efficiency & Optimization • X-tremeGENE™ Customer Test...

EnPresso® B 500 Animal-free Protocol

EnPresso® B is a pre-sterilized growth system designed to increase the yield of functional protein from E. coli-based expression systems. EnPresso growth systems provide optimal conditions for growth, metabolism and protein expression in microbial cultures. Protein yields are increased by enabling c...

New Lab Special Pricing promotion from Sigma-Aldrich

The Sigma-Aldrich® New Lab Start-up Program offers special pricing on thousands of products. Below are highlighted products and product lines within the program that are frequently requested for setting up a new laboratory. Special New Lab Start-Up pricing can be set up for all these products. Chemi...

Solubilization of Membrane Proteins

Related Links • Cell disruption and membrane preparation • Conditioning Purified Membrane Proteins • Performing a purification and on-column refolding of an insoluble histidine-tagged protein from a 100 ml E. coli culture using HisTrap FF 1 ml with ÄKTAprime plus • Performing a purity and homogeneity...

Validation of RNAi Knockdown Using Multiple Reaction Monitoring and Protein-AQUA™

• • Download PDF • Beth K. Radwanski (pictured), Shantanu Roychowdhury, Henry Duewel Research and Development, Biotechnology Division, MiiliporeSigma The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many research...

Locked Nucleic Acid

Locked Nucleic Acid in oligonucleotides other than qPCR probes is available in all countries except for the United States. Locked Nucleic Acid is a novel type of nucleic acid analog that contains a 2'-O, 4'-C methylene bridge (Figure 1). This bridge–locked in the 3'-endo conformation–restricts the f...

Keratinocyte

We are proud to offer a selection of media, many of which are serum-free, for the in vitro culture of human primary cells, such as keratinocytes, endothelial cells, and epithelial hepatocytes. These primary cell types have potential commercial value in a wide range of emerging technologies and thera...

RNAi Tools for Epigenetics Research

Back to BioFiles v7 n3 Main Page Download BioFiles v7 n3 (3.97 Mb PDF) RNA interference (RNAi) is a relatively new technology that is revolutionizing the way researchers study gene expression. RNAi is a highly conserved gene silencing mechanism that occurs in response to the presence of double-stran...