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31 matches found for gene_knockdown

KAPA SYBR® FAST One-Step qRT-PCR Kits FAQs

What are the recommended applications for KAPA SYBR® FAST One-Step qRT-PCR Kits? • Gene expression analysis • Microarray validation • Gene knockdown validation • RNAi and miRNA research What is the difference between a one-step and a two-step qRT-PCR reaction? In a one-step qRT-PCR reaction, reverse...

KAPA SYBR® FAST qPCR Kits FAQs

What are the recommended applications for KAPA SYBR® FAST qPCR Master Mix (2X) Kits? Gene expression analysis • Microarray validation • Low-copy gene detection • Gene knockdown validation • ChIP • Next-generation sequencing library quantification What is the enzyme in the KAPA SYBR® FAST qPCR Master...

Custom & Predesigned esiRNA

All ordering and quotation options are in the esiRNA Specifications table. MISSION esiRNA—powered by Eupheria Biotech--provide RNAi researchers with a proven, cost-effective, and simple way to perform post-transcriptional silencing of protein-coding genes and lncRNA (long non-coding RNA). Biological...

Inducible shRNA Vectors – tight regulation meets great gene silencing

tight regulation meets great gene silencing Request a Quote Background Ordering and Service Information Technical Bulletin Performance IPTG Inducible shRNA References Background Dial into the latest development from the RNAi Consortium (TRC) Constitutively expressed shRNAs are useful for most RNAi n...

Read the latest developments in CRISPR research

read abstract Unconventional Trafficking of Mammalian Phospholipase D3 to Lysosomes Adriana CarolinaGonzalez1MichaelaSchweizer2SebastianJagdmann1ChristianBernreuther3ThomasReinheckel4PaulSaftig1MarkusDamme15 23-01-2018 Variants in the phospholipase D3 (PLD3) gene have genetically been linked to late...

Precision Genetic Modifications

Project Types Our scientists’ years of experience and Sigma-Aldrich’s access to multiple advanced gene editing technologies allow us to offer a cell engineering service with unparalleled success. We can engineer a variety of modifications in your cell line of choice to address your scientific questi...

MISSION® shRNA product overview for plasmid, virus, and glycerol

We are a member and distributor of The RNAi Consortium for phase 1 and 2 (TRC1.5 and TRC2) development of human and mouse lentiviral-based shRNA libraries. The TRC1.5 shRNA collection, comprised of almost 200,000 clones targeting greater than 22,000 human and 20,000 mouse genes, is complete and clon...

Lentiviral Transduction of Suspension Cells - Spinoculation Protocol

The MISSION® TRC shRNA libraries are lentiviral based shRNA vector collections for use in gene knockdown studies. This protocol describes the use of MISSION TRC shRNA Lentiviral Particles particularly for long term silencing and phenotypic observation in suspension cells. The following protocol has ...

Sanger Whole Genome CRISPR Arrayed Libraries

Designed by the Wellcome Trust Sanger Institute Free On-Demand Webinar: Let's Talk Lentivirus Sanger Arrayed Lentiviral CRISPR Libraries won prestigious R&D 100 Award We have joined forces with The Wellcome Sanger Institute to make the first ever arrayed lentiviral CRISPR knockout libraries for huma...

Co-transfection of Plasmid DNA

Georg Hannig & Cordula Jany Roche Diagnostics GmbH Transient co-transfection of plasmids is a method that is commonly employed for cellular protein-protein interaction studies, transcription factor studies, and gene knockdown studies using shRNA encoding plasmids. Thus, co-transfection is useful for...

Targeted Gene Knockdown Experiments: Combining 18O-Isotopic Labeling and Protein-AQUA™

By Jeffrey J. Porter, Beth K. Ewing, Richard J. Mehigh, Justin Wildsmith, Steven L. Cockrill, William K. Kappel, and Graham B.I. Scott Sigma-Aldrich Corporation, St. Louis, MO, USA Targeted gene knockdown using RNA interference (RNAi) has proven to be a powerful tool for studying gene function in eu...

X-tremeGENE™ Transfection Reagents General Recommendations

Related Content • X-tremeGENE™ Transfection Reagents • Roche Biochemical Reagents • Cloning & Expression Products Related Articles • Introduction to Transfection • Transfection Database Related Videos • X-tremeGENE™ Customer Testimony • How Transfection Works • Transfection Efficiency & Optimization...

MISSION™ shRNA Library: Next Generation RNAi

By Stephanie Uder, Henry George, and Betsy Boedeker Sigma-Aldrich Corporation, St. Louis, MO, USA Introduction The technology of RNA interference emerged in its earliest form following a 1998 study in Caenorhabditis elegans1and has since rapidly evolved to its current form as a revolutionary tool fo...

Universal ProbeLibrary System Performance Data

Overview Comparison to Other Assay Formats Comparison to HybProbe Assays on the LightCycler® 2.0 Instrument Comparison to SYBR Green I Assays Comparison to Other Commercially Available Predesigned Custom Assays Comparison to a Commercially Available Hydrolysis Probe Assay Application for Studying Ge...

Targeted Gene RNAi Knockdown Experiments Measured Using AQUA

Richard J. Mehigh, Jeffrey J. Porter, Beth K. Ewing, Justin Wildsmith, Kenneth E. Heuermann, Kelly L. Foster, John G. Dapron, Steven L. Cockrill, William K. Kappel, and Graham B. I. Scott Download pdf version. (1.96 mb PDF) Abstract Introduction Materials Methods Results Conclusions References Abstr...

Biology and Analytical Chemistry Webinar Guide

Biology Webinar Guide Quality Systems and Regulatory Compliance Drug Discovery Webinar Guide Analytical Webinar Guide Materials Science Webinar Guide • Proteomics • qPCR • CRISPR • Cell Culture • Cell Design Studio • Duolink • RNAi • Regulatory Compliance • Late Stage Functionalization of Drug-Like ...

Current References on RNAi

Looking for current application references in the field of RNAi? Look no further. Listed below are many of the top research articles relevant to RNAi and Functional Genomics research. Articles include shRNA, lentiviral-based systems, delivery to various cell lines and transgenic animals, as well as ...

Kapa Biosystems qPCR Reagents and Kits

Quantitative PCR (qPCR) and qRT-PCR reactions require reagents that are accurate and reliable. Kapa Biosystems qPCR and qRT-PCR reagents and kits are designed to provide consistent results for even the most challenging of templates and starting materials. Explore our Kapa Biosystems qPCR reagents an...

Bio Brief: Doxycycline hyclate [VIDEO]

Product No. D9891 Doxycycline hyclate is a semisynthetic tetracycline functioning as a broad spectrum antibiotic and bacteriostatic agent effective against Gram-positive and Gram-negative bacteria. The recommended usage concentration in cell culture applications is 0.1-12 ug/ml. Doxycycline hyclate ...

Short Interfering RNAs (esiRNAs) for Specific and Effective Gene Silencing

Product lists for commonly used protocols from Cold Spring Harbor Protocols. View the entire protocol "Production of Endoribonuclease-Prepared Short Interfering RNAs (esiRNAs) for Specific and Effective Gene Silencing in Mammalian Cells" at BioSupplynet.com Anne-Kristin Heninger and Frank Buchholz A...

MISSION™ shRNA Library: Next Generation RNA Interference

• • Download PDF • The technology of RNA interference emerged in its earliest form following a 1998 study in Caenorhabditis elegans1 and has since rapidly evolved to its current form as a revolutionary tool for studying gene function, biological pathways, and the physiology of disease. Earlier studi...

Functional Genomics & RNAi – siRNA, shRNA and Lentiviral vectors for Biosilencing

We provide leading functional genomics technologies including RNA interference (RNAi) tools for gene silencing (siRNA, esiRNA, shRNA, miRNA) and cutting-edge systems for gene editing and gene knockout. The MISSION® siRNA products offer effective target gene knockdown with reduced off-target effects ...

Pre-arrayed siRNA Libraries

MISSION® Custom siRNA Libraries & Panels are the most compelling collections for high-throughput screening of therapeutic-value target genes. The flexible format facilitates research for scientists who are interested in specific classes of genes as well as those who need to generate information acro...

Predesigned miRNA Inhibitors

All ordering options are in the miRNA Inhibitor SKUs table. MISSION® Predesigned miRNA inhibitors regulate gene expression by binding to and inhibiting specific mature miRNA. The miRNA inhibitors were designed using a proprietary algorithm and the mature miRNA sequence information from miRBase. The ...

Biofiles | Pooled Lentiviral shRNA Screens to Identify Essential Cancer Pathways

• [VIDEO] MISSION® LentiPlex® Pooled shRNA Library Screening in Mammalian Cells Screening with RNAi is a powerful method for perturbing gene activity in cultured cells to enable gene function, pathway analysis, and target identification. shRNA is utilized for stable gene knockdown and two formats ar...

LentiORF Webinar

Whether you are looking to perform gene overexpression studies or validate gene knockdown/knockout results from your RNAi/CRISPR experiments, LentiORFs are your ideal shortcut to protein expression and gene analysis. These genes are available in either pooled or arrayed libraries, and can also be co...

MISSION® shRNA Target Search and Order

Functional Genomics & RNAi MISSION® shRNA Target Search & Order Our MISSION® shRNA Search and Order Tool is your gateway to multi-format RNA interference products designed to support your study of functional genomics through transient or stable gene knockdown (gene silencing). You can search for shR...

Proteomics & Protein Expression Poster Gallery

Featured Poster Use of an Animal-free Synthetic Surrogate Serum Matrix for Preparation of Calibrators and Controls in Immuno- and LC-MS Based Clinical Assays Click on the quick links below to see posters on each topic • Mass Spectrometry Tools • ProteoPrep™ Plasma Immunodepletion • NEW! SILu™Prot Ma...

MISSION® TRC3 Human ORF Collection

Our ready-to-use MISSION® TRC3 Open Reading Frame (ORF) clones allow for stable integration, enrichment of cells, and long-term gene expression in difficult-to-transfect cell lines utilizing our best-in-class manufacturing. This collection provides researchers with the unique tools to gain insights ...

siRNA Delivery in Mammalian Cell Lines

Mads Daugaard* and Nicole Fehrenbacher The Apoptosis Laboratory, Institute of Cancer Biology, The Danish Cancer Society, Copenhagen, Denmark *Corresponding author: mdj@cancer.dk RNA interference (RNAi) is a mechanism of posttranscriptional gene silencing effective in most eukaryotic organisms. The R...