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362 matches found for genetic

RNAi Glossary

Antisense molecules Argonaute Cosuppression Drug target Dicer Expression Functional Genomics Gag Gene silencing Gene target Genomics Interferon response Knock-down Long terminal repeat (LTR) MicroRNA (miRNA) Multiplicity of infection (MOI) Off-target effects Packaging plasmid Phenotype Pol Pol III p...

Media for Vaccine Production & Medium Supplements

Cultured cells have long been known to serve as excellent hosts for propagation of many types of viruses. The ability of cell culture systems to produce large quantities of attenuated viral particles has served as the basis for the production of both human and veterinary vaccines. Traditional method...

Regulatory Agencies & Conclusions - PCR Technologies Guide

PCR Technologies Guide Historical Timeline Polymerase Chain Reaction Quantitative PCR Digital PCR Quantitative PCR and Digital PCR Detection Methods PCR/qPCR/dPCR Assay Design Sample Purification and Quality Assessment Reverse Transcription Assay Optimization and Validation Data Analysis Troubleshoo...

Extract-N-Amp Blood Kit

High-throughput genetic analysis of blood requires a rapid, automated solution for genomic DNA extraction. Standard methods for purifying DNA from blood can be laborious, not amenable to automation, and unnecessary for robust downstream techniques such as PCR. The Extract-N-Amp Blood PCR Kit is a hi...

Cell Culture Collections: Exploring the Iceberg

• • Download PDF • Biowire Spring 2011 — Cell Lines — Models of Disease Cell line misidentification and Mycoplasma contamination are well known as the greatest risks to any practitioner of cell culture1,2. Biological resource centers such as the European Collection of Cell Cultures (ECACC®) exist to...

TransPlex

View Other Articles | Download a PDF of this article TransPlex® RNA Amplification Kit TransPlex Complete Whole Transcriptome Amplification Kit: Whole Transcriptome Amplification of Highly-Degraded RNA from FFPE Tissues Ken Heuermann and Brian Ward Research and Development, Biotechnology Division, Si...

Food Microbiology Testing

Discover our Microbiology Testing Solutions for the Food Industry. Hot Topics • Short talk: Compliant detection and enumeration of Campylobacter spp. • On-demand webinar: Food microbiology regulatory update - new & revised ISO 11133 and ISO 17410 standards. • Ask the expert: How to Choose the Right ...

Landing Pad ZFN-Modified Cell Line Purchase Agreement

This product (“Product”) and its use is the subject of one or more of the following patents controlled by Sangamo BioSciences, Inc.: U.S. Patent Nos. 6,534,261, 6,607,882, 6,746,838, 6,794,136, 6,824,978, 6,866,997, 6,933,113, 6,979,539, 7,013,219, 7,030,215, 7,220,719, 7,241,573, 7,241,574, 7,585,8...

Epigenetic Mechanisms in Cancer Pathogenesis

Cancer accounted for approximately 13% of all deaths in 2004, according to the cancer fact sheet published by the World Health Organization (WHO) in February 2009. The term cancer (malignancy) describes a diverse class of diseases characterized by uncontrolled cell growth, which frequently culminate...

Whole Genome Amplification for Single Cell Biology - Technical Article

The ultimate biological unit lies within a single cell. Many biological disciplines have taken aim to elucidate the causes of cellular differentiation at this level. The secret triggers that signal human maturation, regeneration and genetic diseases all lie buried in a single cell that was originall...

Products for Angiogenesis, Antitumor Agents, Cancer Markers, Carcinogens, Chemopreventive Agents

During the last twenty years our understanding of the biology of cancer has increased dramatically. It is now widely recognized that cancer results from a series of genetic alterations causing a loss of normal growth controls, resulting in unregulated growth, lack of differentiation, apoptosis, geno...

Read the latest developments in CRISPR research

read abstract Unconventional Trafficking of Mammalian Phospholipase D3 to Lysosomes Adriana CarolinaGonzalez1MichaelaSchweizer2SebastianJagdmann1ChristianBernreuther3ThomasReinheckel4PaulSaftig1MarkusDamme15 23-01-2018 Variants in the phospholipase D3 (PLD3) gene have genetically been linked to late...

Video - Advanced Cell Culture Technology for Generation of In Vivo-like Tissue Models

Sponsored by the European Collection of Authenticated Cell Cultures (ECACC) More sophisticated cell models are developing as 3D cell culture technology becomes established and accepted as a means of creating more physiologically relevant cell-based assays. Methods that are relatively straightforward...

Oncology Cell Lines

Cells and Cell-Based Assays Oncology Cell Lines We have created a range of human cell lines with targeted and heritable gene deletions and mutations using our ground-breaking CompoZr® ZFN Technology. An individual patient's response to therapy may vary depending on their unique genotype. To better u...

Introduction to Cell Transfection

Related Content • X-tremeGENE™ Transfection Reagents • Roche Biochemical Reagents • Cloning & Expression Portfolio • X-tremeGENE™ Transfection Database • X-tremeGENE™ General Recommendations • Transfection Best Practices Related Videos • How Transfection Works • Transfection Efficiency & Optimizatio...

KAPA2G Fast PCR Kits FAQ

What are the recommended applications for KAPA2G Fast PCR Kits? • Standard, end-point PCR assays normally performed with wild-type Taq (or hot-start formulations thereof) – but completed in 20%–70% less time • High-throughput end-point PCR • End-point PCR for genetic testing or downstream Sanger seq...

shRNA Frequently Asked Questions for Lentiviral Particles

Which packaging systems are compatible with pLKO.1 shRNA vectors? Do you offer any cells for packaging lentivirus? What is the titer for the Lentiviral transduction particles? Is a lentiviral titer of 106 too low? How do you determine functional viral titer (TU/ml) using the p24 assay? How serious a...

Whole Genome Amplification: Extraction Protocol - Whole Blood

Whole blood is a common source of material used to perform genetic analysis. Many times genomic DNA isolated from whole blood samples is of low yield. This can hinder the researcher’s ability to perform downstream analysis. The following protocol is a simple method to isolate DNA from fresh or aged ...

TRC Library Citations

Publish Sooner When You Partner with the MISSION® shRNA Team! The MISSION® TRC1.5 human and mouse shRNA libraries are the proven performers for small-scale to high-throughput RNAi screening. Below you will find a selection of papers where researchers have published in peer-reviewed journals using th...

Whole Gene Amplification from Archived Formalinfixed, Paraffin-embedded Tissues

Application Notes • High yield from limited template – Amplify nanogram amounts of genomic DNA into microgram yields in less than three hours • Preserve precious samples – Only ten nanograms of starting material required • Amplify DNA from any source – Suitable with numerous sources of DNA including...

Stem Cell Biology — Where We’ve Been and Where We’re Heading

BioFiles 2008, 3.11, 4. Dr. Greg Wall, Ph.D. Unraveling and exploring the mysteries of life have been driving forces behind scientific studies since Aristotle first attempted to understand the origins of life. Through the eyes of the microscope, cellular processes have been observed, investigated, u...

Genome by Design

Without a robust understanding of what is being observed, no conclusions can be drawn. In biology, the multifaceted nature of even basic in vitro systems makes this a complex task, requiring not just stringent control of as many variables as possible, but also an appreciation of the likely impact of...

Enantiomeric Separation & Analysis of Native or Derivatized Amino Acids using Chiral HPLC and Mass Spectrometry

Ric Cone and Wayne K. Way ric.cone@sial.com Separating native and derivatized amino acid enantiomers or diastereomers using chiral HPLC is integral to research in the pharmaceutical, bioscience, medical diagnostic, food, and agricultural sectors. Amino acids contain an asymmetric center, and determi...

Flow Cytometry Webinars

Related Links • Flow Cytometry Instruments • Amnis® Imaging Flow Cytometers • Amnis® Resources • Muse® Cell Analyzer • Guava® Flow Cytometers • CellStream™ Benchtop Flow Cytometers • Applications of imaging flow cytometry for the diagnosis of leukaemia • ToxTracker Assay for High-throughput Screenin...

CRISPR

In recent years CRISPR has revolutionized gene editing capabilities, leading to sophisticated ways to create success with any experiment. As the first company to offer custom biomolecules globally for genome editing, we are trusted worldwide for the latest, most innovative solutions in CRISPR/Cas9 p...

GenomePlex® Products | Whole Genome Amplification

Sigma-Aldrich has a dedicated team of R&D scientists committed to providing you best-in-class products for whole genome amplification. We continue to expand and enhance our WGA product line to offer you a range of solutions for whole genome amplification from a variety of starting material. The WGA ...

DNA Damage and Repair

Related Products • DNA Repair Regulators • DNA Repair Proteins and Reagents • Antibodies to DNA Repair Proteins Related Genes • PMS1 • MLH1 • MLH3 • MSH2 • MSH3 DNA Damage and Repair Mechanisms Damage to cellular DNA is involved in mutagenesis and the development of cancer. The DNA in a human cell u...

Expression of the Multiple Sclerosis-Associated MHC Class II Allele HLA-DRB1*1501 Is Regulated by Vitamin D

• • Download PDF • 1Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom, 2 Department of Clinical Neurology, University of Oxford, John Radcliffe Hospital, Oxford, United Kingdom,3 Department of Medical Genetics, Division of Neurology, University of British Columbi...

Plant Molecular Biology: Your source for Plant Products & Reagents

Plant Molecular Biology includes products for plant nucleic acid purification, isolation and amplification. Plant Molecular Biology products support PCR, genotyping, genetic mapping, sequencing, cloning, gene expression and analysis, and protein expression and analysis. The product highlights sectio...

Viral Safety

Viral safety assurance strategies Read the most recent papers read abstract Adapting viral safety assurance strategies to continuous processing of biological products. SA Johnson,MR Brown,SC Lute,KA Brorson Biotechnology and bioengineering 2017-12-16 Read More read abstract Vesivirus 2117: Cell line...