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296 matches found for growth_factors

Biodegradable Polyester-based Nanoparticle Formation by Miniemulsion Technique

Anna Musyanovych and Katharina Landfester Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany Email: landfest@mpip-mainz.mpg.de Aliphatic polyesters such as polylactide, poly(lactide-co-glycolide) and polycaprolactone, as well as their copolymers, represent a diverse fam...

Chitosan: A Versatile Platform for Pharmaceutical Applications

Raphael Riva and Christine Jerome* Center for Education and Research on Macromolecules University of Liege, Chemistry Department, Sart-Tilman, Building B6a, 4000 Liege, Belgium *Email: c.jerome@ulg.ac.be The development of new medical devices and pharmaceuticals plays an integral role in the medical...

Cultrex® 3-D Culture Matrix™ Basement Membrane Extract Coated 96 Well Plate Protocol

Product Number 3445-096-CP 3-D Culture is an innovative approach to modeling the morphological effects of oncogenesis and development using three-dimensional microenvironments. Extracellular matrix proteins form hydrogels under physiological conditions that mimic the cell environment in vivo, and as...

HIC as a Capture Step

Appendix 1, extracted from Hydrophobic Interaction and Reversed Phase Chromatography Principles and Methods (PDF), GE Healthcare, 2014 The objective of a capture step is to quickly bind the protein(s) of interest from the crude sample and isolate them from critical contaminants such as proteases and...

Managing Risks within Platform Processes through Media Components

Introduction Supplier Risk Raw Material Risks Process Risks Mitigating Risks View the PDF A platform process is a set of standardized protocols and reagents that can be used to support a variety of clinical molecules. Typically these protocols and reagents, including cell culture media and feeds, ar...

Propolis (Propolis) | Plant Profiler

Synonyms / Common Names / Related Terms Apis mellifera L., bee glue, bee propolis, bee putty, Bienenharz (German), Brazilian green propolis, Brazilian propolis, Bulgarian propolis, caffeic acid phenethyl ester, CAPE, cera alba, chizukit, cinnamic acid, flavonoids, galangin, Greek propolis, hive dros...

AvaBridge ELISA Kit Protocol

Product Number. ABK0001 Complete kit for the systematic 3-D conformational comparability analysis of Avastin biosimilar molecule to Bevacizumab (Avastin trade name). Read the entire protocol before performing the assay. This kit is intended for research use only and not for use in diagnostic procedu...

Growth Factors and Cytokines Review

Jennifer Fries Technical Marketing Specialist, Cell Culture The importance of cytokines and growth factors in cell culture has been demonstrated time and time again since the inception of cell culture. Beginning with the first successful efforts of growing cells in culture, there have been so called...

EGF/EGFR signaling pathway

Background Epidermal Growth Factor Receptors (EGFR) and ligands Receptor activation EGFR Signaling Ras/Raf signaling cascade Phosphatidylinositol 3-kinase/Akt signaling cascade Signal transducers and activators of transcription (STAT) pathway Phospholipase Cγ signaling Nck/PAK signaling cascade Cbl ...

placental growth factor
Synonyms D12S1900, PGFL, PLGF, PlGF-2, SHGC-10760
Species Human (5228) , Species Mouse (18654) , Species Rat (94203) , Species chicken (771832) , Species dog (611916) , Species domestic cat (101095904) , Species naked mole-rat (101725085) , Species domestic guinea pig (100734450) , Species cow (280894)
Summary: This gene encodes a growth factor found in placenta which is homologous to vascular endothelial growth factor. Alternatively spliced transcripts encoding different isoforms have been found for this gene.[provided by RefSeq, Jun 2011]

Cultrex® 3-D Culture Matrix™ Laminin I Protocol

Product Number 3446-005-01 3D Culture is a cell culture method that provides cells with the necessary structure and signaling cues to direct reconstruction of the tissue architecture, and these methods provide physiologically predictive in vitro models for evaluating development and disease. Normal ...

How to Optimize the Duolink® Proximity Ligation Assay

Related Products • Duolink® PLA • Primary Antibodies Related Content • Duolink® PLA - Protein Detection Technology • Duolink® PLA Resource Center To successfully execute a Duolink® PLA experiment, there are some necessary considerations to properly prepare for, setup, and execute the assay protocol....

Tailoring Collagen-Based Matrices for Regenerative Medicine and Tissue Engineering

Jiayu Leong,1 Liam Grover,2 Hyunjoon Kong1* 1Department of Chemical and Biomolecular Engineering University of Illinois, Urbana-Champaign, IL 61801 USA 2University of Birmingham, UK *Email: hjkong06@illinois.edu Collagen molecules play a critical role in tissue architecture and strength, and in cell...

MAPKs

The mitogen-activated protein kinase (MAPK) family consists of both stress activated (SAPK) and mitogen-activated (MAPK) protein kinases. They form a network of signal transduction cascades that mediate cellular responses to a diverse range of stimuli, including growth factors, chemical or osmotic s...

Chitosan-based Biomaterials

Chitin, a natural polysaccharide, is the second most abundant natural biopolymer in the world, after cellulose. It is the primary, high modulus fibrous component of the exoskeleton of arthropods including crab, shrimp and lobsters, as well as some fungi. Structurally, chitin is an insoluble, linear,...

The Tumor Microenvironment

Several recent reviews underscore the contribution of the microenvironment to tumor development.12,15-18 While the normal cellular microenvironment can inhibit malignant cell growth, the modifications that occur in the tumor microenvironment synergistically support cell proliferation. Tumors shape t...

Growth Factors and Cytokines: Classification and Nomenclature

• • Download PDF • Growth factors and cytokines have historically been classified into ‘families’ based on their apparent activity and/or impact on a given cell type, system, or tissue. Lately however, there has been an effort to establish naming based upon growth factor and cytokine receptors. Grow...

Induced Pluripotent Stem Cell Reprogramming Protocols

Related Products • Pluripotent Stem Cell Culture • Stem Cell Culture Media • Stem Cell Protocols • MEF Feeder Cells • 3D Cell Culture • Primary Cell Culture • ECACC Cancer Cell Lines • Cell Culture Media and Reagents • Stericup® Media Filtration • Growth Factors • ECM Protein • Antibodies • Bioactiv...

ECM Gel Matrix: Protocols Using EHS Basement Membrane Extracts

Related Products • 3D Cell Culture Tools and Applications • 3D Cell Culture 101 • 3D Hydrogels • TrueGel3D ™ Synthetic Hydrogel Platform • 3D Scaffolds • Spheroid Cell Culture Media • ECM Attachment Factors • Stem Cell Culture • ECACC Cell Lines • Primary Cell Culture • Cell Culture Media • Cell Cul...

Assessing Enriched Murine CD4+ T Cells Differentiated Towards Effector T Helper Cell Lineages

Cellular differentiation, the process by which progenitor cells differentiate into specialized cell types, is fundamental to developmental biology. In the immune system, immune progenitor cells must differentiate into diverse innate and adaptive phenotypes for the body to respond to infections. In t...

Sonic Hedgehog: Its dual role in morphogenic and mitogenic signaling

Related Links • Antibody Explorer: Search primary and secondary antibodies • Prestige Antibodies • Antibody Resource Center Mammalian Hedgehog proteins include Sonic Hedgehog (Shh), Indian Hedgehog (Ihh), and Desert Hedgehog (Dhh). They are involved in regulating pattern formation during embryonic d...

Microcon® Centrifugal Filters

Simply and efficiently concentrate and desalt solutions of any macromolecule, using any centrifuge that can accept 1.5 mL tubes. With the low-binding Ultracel® membrane, Microcon® filters offer: • Typical recoveries of >95%, even for dilute solutions • Reverse spin to maximize recovery, even in the ...

LONG®R3IGF-I: An Insulin Alternative Exclusively for Cell Culture Applications

• • Download PDF • LONG®R3IGF-I is a recombinant analog of human insulin-like growth factor-I (IGF-I) that has been specifically engineered for the enhancement of cell culture performance. LONG®R3IGF-I is more biologically potent in vitro than either insulin or native IGF-I and has been shown to sig...

Microcon® Centrifugal Filters Applications Guidelines & Specifications

Microcon® Centrifugal Filters simply and efficiently concentrate and desalt solutions of DNA, RNA, protein or other macromolecules, using any centrifuge that can accept 1.5 mL tubes. With the low- binding Ultracel® membrane, Microcon® filters offer: • Typical recoveries of >95%, even for dilute solu...

Met

The Met family of receptor tyrosine kinases (RTKs) contains two members in mammals, referred to as Met and Ron. Met is the receptor for hepatocyte growth factor/scatter factor (HGF/SF), while Ron is the receptor for macrophage stimulating protein (MSP). In the adult, Met is predominantly expressed i...

Authenticated Lung Cancer Cell Lines for Cancer Research

Related Links • Cell Culture • ECACC Cell Lines • Protocols for ECACC Cell Lines • Browse Lung Cancer Cell Lines Related Articles • Non-Small Cell Lung Cancer • Lung Cancer & IHC Webinar • Breast Cancer Cell Lines • Colorectal Cancer Cell Lines • Liver Cancer Cell Lines • Pancreatic Cancer Cell Line...

Cell Signaling & Neuroscience Literature

• Alzheimer's Disease Pathways poster • Cytoskeleton & Extracellular Matrix brochure • Gene Regulation and Chromatin Modification brochure • Ion Channel Solutions brochure • Ion Channels Technical brochure • Neurodegenerative Diseases brochure • Neuropsychiatric Disorders brochure • Tyrosine Kinases...

Purification or Removal of DNA-Binding Proteins

Extracted from Affinity Chromatography Vol. 3: Specific Groups of Biomolecules, GE Healthcare, 2014 DNA-binding proteins form an extremely diverse class of proteins sharing a single characteristic, their ability to bind to DNA. Functionally the group can be divided into those responsible for the repli...

Genome engineering using the CRISPR-Cas9 system

Nature Protocols Volume 8 Issue 11, 24 November 2013, Pages 2281 - 2308 doi: 10.1038/nprot.2013.143 Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. F Ann Ran a, b, c, d, e , Patrick D Hsu a, b, c, d, e , Jason Wright a , Vineeta Agarwala a, f, g , David A Sc...

Electrospun Nanofibers for Drug Delivery Systems

Sang Jin Lee*, James J. Yoo, and Anthony Atala Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine Medical Center Boulevard, Winston-Salem, NC 27157, USA *Email: sjlee@wakehealth.edu Local delivery of bioactive molecules using an implantable device can decrease the amount...

Growth Factors in Stem Cell Biology

Related Links • Growth factors • 3D-cell culture • Organoids • Pluripotent Stem Cell Culture • Stem cell basics Related Products • Cell Culture Media and Reagents • Growth Factors • Stem Cell Antibodies • CRISPR Genome Editing • 3D Cell Culture • ECACC cell lines • 3DKUBE • 3D Hydrogels Growth facto...