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    Search term:"high_performance_liquid_chromatography"

    1014 matches found for high_performance_liquid_chromatography

    Microcon® Centrifugal Filters Applications Guidelines & Specifications

    Microcon® Centrifugal Filters simply and efficiently concentrate and desalt solutions of DNA, RNA, protein or other macromolecules, using any centrifuge that can accept 1.5 mL tubes. With the low- binding Ultracel® membrane, Microcon® filters offer: • Typical recoveries of >95%, even for dilute solu...

    Molecular Beacons Applications, Benefits & Products

    Molecular Beacons are structured probes that are highly sensitive, sequence specific, and are used for sequence detection in qPCR and in vitro studies. • When to Choose Molecular Beacons • Benefits of Using Molecular Beacons • Add LNA® to Your Probe • How Molecular Beacons Work • Product Features • ...

    Acetone - High Purity Solvents

    Whether you need HPLC acetone, ACS acetone or another Sigma-Aldrich grade, we stock the right product for your application. As a leading supplier of high-purity, research grade solvents, we have the acetone to meet your exact needs. View and Compare Acetone Products Acetone Physical Properties Synon...

    Proteomics Core Partnership Program

    Be at the forefront of Proteomics Research! With our industry-leading investments and technical expertise in cutting-edge, innovative Protein Mass Spectrometry technologies, we provide you the most diverse and advanced Proteomic workflow solutions on the market. Your membership to our reputable Prot...

    Acetonitrile - High Purity Solvents

    Whether you need HPLC acetonitrile, BioTech acetonitrile, Anhydrous acetonitrile or another Sigma-Aldrich grade, we stock the right product for your application. As a leading supplier of high-purity, research grade solvents, we have the acetonitrile to meet your exact needs. View and Compare Acetoni...

    Food & Beverage Analysis: Sugars & Saccharides

    Related Links • Carbohydrate Analytical Standards • apHera™ NH2 column • Ascentis® Express • SUPELCOGEL™ • SUPELCOGEL C-611 • Enzymatic Food Kits and Reagents Related Articles • Improved Analysis of Simple Sugars Using apHera™ NH2 HPLC Columns • Determination of Additives in Beverages Using Ascentis...

    Testing Food for Fipronil Contamination

    Related Products • Analytical Standards • Certified Reference Materials • Environmental Pesticide Standards • QuEChERS • LC-MS • GC-MS Additional information • Application: Analysis of Fipronil in Eggs • Fipronil Flyer • EFSA report: Fipronil: Results of follow-up monitoring (published 03 May 2018) ...

    Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

    Extracted from Affinity Chromatography Vol. 1: Antibodies, GE Healthcare, 2016 Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step. GE o...

    Arnica (Arnica montana) | Plant Profiler

    Synonyms / Common Names / Related Terms 6-methoxykaempferol, Aconitum napellus, alisma, American arnica, Arnica augustifolia, Arnica chamissonis, Arnica cordifolia, arnica da serra, arnica flower, Arnica fulgens, Arnica latifolia, Arnica lonchophylla, Arnica montana, arnica root, Arnica sororia, arn...

    Milk Protein CRM

    Related Links • View Product List • Food and Beverage Home • Analytical Standards and Reference Materials • LC-MS • Food Microbiological Control • Food Chemical Analysis Mothers’ own milk is the best source of nutrition for nearly all infants. Beyond somatic growth, breast milk as a biologic fluid h...

    CRISPR Essentials

    This CRISPR Essentials page will help you identify which genome editing vector and format best fit your needs. Use the vector tables to compare and contrast components in a variety of systems. The vector maps and sequence files provide visual references to further assist in choosing the right compon...

    Peptide Mapping with Ascentis Express Peptide ES-C18

    Related Articles • Faster Protein and Peptide Liquid Chromatography: Introducing BIOshell Fused-Core HPLC Columns Hillel Brandes hillel.brandes@sial.com Peptide mapping is an established technique for assessing changes to the primary structure of a protein. This has applications in areas of quality ...

    Agro, Food and Beverage

    We are offering a wide range of solvents for the Food and Beverage, covering qualitative and quantitative food safety analysis, composition/nutritional testing and food production. An overview on all products and services for the agro, food and beverage market can be found on the Food & Beverage mai...

    Proligo Manufacturing Site | Hamburg

    State-of-the-Art Production The Proligo® manufacturing site in Hamburg, Germany is designed as a multi-purpose facility with built-in flexibility and the capability to manufacture a complex mix of products to suit a variety of customer needs. DNA and RNA phosphoramidites are manufactured in glass-li...

    Semi-Bulk Packaging and Dispensing

    Sigma-Aldrich offers a variety of packaging options for those applications when individual bottles are not practical. With larger volumes of solvents, safe and proper dispensing methods are essential. View our Complete List of Semi-Bulk Solvent Packaging Options, Drum Accessories, and Aldrich VerSA-...

    Frequently Asked Questions about Solvents in Hematology & Histology Research

    General Information Tax-Paid Ethanol Physical Properties Application Notes Returnable Containers General Information How do I get specific lot information or CofA for a solvent? A Certificate of Analysis is available by lot for each of our solvents and can be accessed through our Advanced Search Opt...

    ChemMatrix® Resin – A major advance in solid phase peptide synthesis

    • • Download PDF • Matthias Junkers, Ph.D. Product Manager matthias.junkers@sial.com ChemMatrix® is a proprietary, 100% PEG (polyethylene glycol) based resin from PCAS BioMatrix. It combines the strengths of two major resin systems — the chemical stability of polystyrene resins and the superior perf...

    When C18 is Not Quite Enough in HPLC...

    Denise Wallworth denise.wallworth@sial.com The automatic column choice in reversed phase HPLC is traditionally a C18 or perhaps a C8, but this does not always provide the optimum selectivity desired – it might not always have the best selectivity or the elution order that is required. Whether the co...

    Protein AQUA™ Applications - Verify gene silencing, quantitate low-abundance proteins.

    Verify Gene Silencing Verification of gene silencing represents a novel and powerful application of the AQUA technique. Recently, Jeffrey Porter and his team at Sigma-Aldrich demonstrated that AQUA quantitation is an effective method for measuring expression of silenced genes and verifying knockdown...

    A Comparison of Peptides to Whole-Protein Stable Isotope Labeled Internal Standards in Quantitative Protein MS Workflows

    Characterize a stable isotope labeled human erythropoietin (SILu™Prot EPO) and compare it’s use as an internal standard in an immunoaffinity enrichment experiment to the SIL peptide approach. LC-MS/MS methods employing the sensitivity of multiple reaction monitoring (MRM) for quantification of prote...

    Hematology & Histology Renal Transplant Article at Sigma-Aldrich.

    Ranjitha Veerappan, MD, Lou Ellen Miller, HT (ASCP), Piyush Joshi, MD, and George A. Youngberg, MD This article has been reprinted with permission from HistoLogic, vol 37 no. 2, 2004 On this page: Introduction Case Report Methods Results Discussion References Introduction: Determining if the cause o...

    Troubleshooting & Frequently Asked Questions for HybridSPE-PL

    Yes. The HybridSPE-PL Small Volume plate (52794-U) is designed for processing plasma/ serum volumes between 20-40 µL. Larger sample volumes of 100-300 µL should be processed on the standard HybridSPE-PL plate (575656-U). Acetonitrile is a commonly used protein precipitation agent when prepping plasm...

    Enzyme Class Index: Hydrolases other

    Sigma offers over 1,600 enzymes as well as associated inhibitors and substrates. TO SEARCH FOR AN ENZYME BY EC # OR REACTION CATALYZED... 1. Click on a category in the menu below 2. Scroll down the list to the enzyme of interest 3. Click on the Product # to access product listing and related informa...

    Supel-Select HLB SPE for Pharmaceutical Analysis

    Related Articles UHPLC Analysis of Taxanes Using A Titan C18 Column and UV Detection Fast HPLC Using Fused-Core Particle Technology for Rapid Screening of Pharmaceutical Compounds Metabolites – A Serious Challenge for LC/MS Separations and Usual Detection Techniques Serum Lipidomics Profiling using ...

    Glycoprotein Deglycosylation

    • • Download PDF • Aparagine-linked (N-linked) and serine/threonine-linked (O-linked) oligosaccharides are major structural components of many eukaryotic proteins. They perform critical biological functions in protein sorting, immune recognition, receptor binding, inflammation, pathogenicity, and ma...

    Phytochemical Standards

    Sigma-Aldrich’s offering of phytochemical analytical standards consists of natural products ranging from alcohols and phenols, aldehydes and ketones, alkaloids, flavonoids, glucosinolates, and isoprenoids to organic acids and esters, phenylpropanes, quinones, and tannins. These reference substances ...

    Evaluation of LC-MS MS Scrambling Ratios for Deuterium-Labeled Vitamin D Metabolites Steroids and Other Compounds of Clinical Significance

    Introduction and Objective: A significant clinical challenge with LC-MS/MS is the potential for matrix effects that cause interferences or impact ionization efficiency. Stable isotope-labeled internal standards are frequently used to compensate for matrix effects and to increase the accuracy of quan...

    Suzuki-Miyaura Cross-Coupling of Potassium Dioxolanylethyltrifluoroborate and Aryl/Heteroaryl Chlorides

    A robust and general catalyst system�facilitates the alkoxylation of activated heteroaryl halides with primary, secondary, and select tertiary alcohols without the need for an excess of either coupling partner (see scheme). This catalyst system displays broad functional-group tolerance and excellent...

    MSMLSDiscovery™ Software Manual

    MSMLSDiscovery™ is a software tool designed to streamline and simplify the tedious process of building authentic standard libraries for mass spectrometry. The program is tailored to work with the Mass Spectrometry Metabolite Library of Standards (MSMLS) distributed by IROA technologies. Future relea...

    Technical Resources for Ascentis Express

    Brochures Product Information Care and Use Guides Technical Articles Applications Ascentis Express Brochure (view / request) Ascentis Express Resource Guide (view) Ascentis Express 5 µm Brochure (view) Ascentis Express HILIC Guide (view) Publication Title Type Size (kb) No. Pages Brochures back to t...