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180 matches found for indicators

Identification of Microorganisms Based on Color

For microbiologists the most fundamental stain was developed in 1884 by the Danish bacteriologist Hans Christian Gram. The Gram Stain allows the determination of morphology, dividing bacteria into two large groups. Bacteria that are stained purple are called “Grampositive”. Those that stain pink are...

Comparing Quantitative Viability Bioassays: An Evaluation of MTT, alamarBlue™, and Guava® ViaCount® Methods

Krista McCutcheon and David Fei, Department of Analytical Sciences, Genentech Inc., South San Francisco, CA 94080-4990 Viability assays are crucial tools in the development of many drugs, particularly of cytotoxic agents. To best characterize potential compounds, viability assays must ideally be abl...

Perform Titrations with the BRAND® Titrette®

When titration is a critical, repetitive part of work either in process monitoring during production, quality control in chemistry and pharmaceutical industries or in research and development, two common properties are desirable: The equipment used should be easy and reliable to operate and the resu...

Rooibos (Aspalathus linearis) | Plant Profiler

Synonyms / Common Names / Related Terms Aspalathin, Aspalathus acuminatus, Aspalathus contaminata, Borbornia pinfolia, chrysoeryol, Fabaceae/Leguminosae (family), hyperoside, isoorientin, isoquercitrin, isovitexin, Kaffree tea, long life tea, luteolin, orientin, Psoralea linearis, quercetin, (R)-eri...

Solubilization of Membrane Proteins

Related Links • Cell disruption and membrane preparation • Conditioning Purified Membrane Proteins • Performing a purification and on-column refolding of an insoluble histidine-tagged protein from a 100 ml E. coli culture using HisTrap FF 1 ml with ÄKTAprime plus • Performing a purity and homogeneity...

Alternative Culture Media Methods

Our range of rapid culture media plates are an efficient method for the detection and enumeration of microorganisms including quality indicators, pathogens and spoilage organisms. Validated by AOAC/MicroVal, these methods give a clear read-out and our SimPlate® solutions are particularly effective w...

Salmonella Contamination and Testing

Related Links • View Product List • Industrial Microbiology Testing Portfolio Related Information • Food and Beverage Home • Food Microbiological Control • ISO 6579 – Salmonella Detection • ISO 11133 – Food Microbiological Testing • GranuCult™ Culture Media Foodborne illness affects public health an...

Custom Oligos for Commercial Use

We offer custom DNA Primers, qPCR Probes, and Next-Gen Sequencing Oligos for Life Science Research Tools, Molecular Diagnostics, and Laboratory Developed Tests. We can also perform component or complete kit manufacturing, including custom formulations, packaging, and private labeling. Learn about ou...

Featured article on Peroxide–forming Solvents used in research offered by Sigma-Aldrich.

A significant number of laboratory solvents can undergo autoxidation under normal storage conditions to form unstable and potentially dangerous peroxide by-products. This process is catalyzed by light and heat and occurs when susceptible materials are exposed to atmospheric oxygen. Molecular structu...

lysine (K)-specific demethylase 8
Synonyms JMJD5
Species Human (79831) , Species Mouse (77035) , Species Rat (308976) , Species Zebrafish (436936) , Species cow (507905) , Species chicken (416583) , Species domestic cat (101096528) , Species domestic guinea pig (100723684) , Species naked mole-rat (101702352) , Species sheep (101108800)
Summary: This gene likely encodes a histone lysine demethylase. Studies of a similar protein in mouse indicate a potential role for this protein as a tumor suppressor. Alternatively spliced transcript variants have been described.[provided by RefSeq, Feb 2009]

Sensor Dyes

In 1998 R.F. Furchgott, L.J. Ignarro, and F. Murad received the Nobel Prize in Physiology and Medicine for their discoveries concerning nitric oxide as a signaling molecule in the cardiovascular system. This indicates the importance the scientific community has placed on the role of nitric oxide (NO...

Epigenetics Overview - A portfolio of products for each step of your Epigenetic research workflow.

Epigenetics is a term coined to describe changes that are not mutation based but can still be passed on from generation to generation. Genes that are activated or repressed without any change in DNA sequence are epigenetically controlled. Epigenetic modifications are stable, but potentially reversib...

MSX Amplification

The Glutamine Synthetase (GS) expression system does not typically require multiple rounds of amplification to isolate high-producing clones (Brown, 1992). However, most Chinese Hamster Ovary (CHO) cell production lines contain endogenous GS, and Methionine Sulfoximine (MSX) is required to inhibit e...

Fiery Flavors: Consumers seek bolder flavors, yet still demand full transparency

Related Links • Flavors & Fragrances Learning Center Interested in receiving more content like this? Join over 2,500 of your peers who have already signed up for the bi-monthly Flavors and Fragrances newsletter. As U.S. consumers’ demands for flavor become more sophisticated, food merchants are resp...

Mesoporous Silica and their Applications

Since their discovery in the late 1970s, mesoporous silica have attracted quite a bit of attention. This is mainly due to their unique properties such as ordered pore structures, very high specific surface areas and their possible synthesis in a wide range of morphologies such as spheres rods, discs...

EZ-Fit® Filtration Units for Bioburden Testing

Related Products • Microbiology Equipment • Beverage & Water microbiological control • Food microbiological control • Small molecule pharma QC workflow • EZ product family • Bottled water analysis • Microbiology Equipment • Microbiology Media Related Resources • Brochure: EZ Family and ReadyPlate™ 5...

Fluorous Separation Media - Fluorous Chemistry

The separation of fluorous compounds from non-fluorous compounds using fluorous silica gel is the central theme of fluorous techniques. Fluorous solid phase extraction (F-SPE) is used for quick separations of reaction mixtures involving FluoroFlash® tags, scavengers, and reagents. The separation of ...

3S Reagents for Volumetric Titration

Connect Your Lab 3S is an intelligent packaging solution protected with an originality seal. The cap provides information via Radio-frequency identification or RFID, enabling automated data transfer between the reagent and titration instrument. Intelligent communication for improved performance. Tit...

Bind/Elute vs Flowthrough Mode in Multimodal Chromatography

Extracted from Multimodal Chromatography (PDF), GE Healthcare, 2013 In multimodal chromatography, the choice between bind/elute and flowthrough mode is more complex than when using a single method, such as IEX, because multiple types of interactions are occurring in multimodal chromatography, and th...

The Effect of Sample Concentration and Complexity on SPME Fiber Selection

Related Articles • Development of a Method for Analysis of Phthalate Esters in Vegetable Oils • SPME Method for 4-methyl-1-cyclohexanemethanol (MCHM) and propylene glycol phenyl ether (PPH) in Water Bob Shirey bob.shirey@sial.com Previously, in the Reporter (Vol 24.3) we discussed how to select the ...

Benchtop flow cytometry facilitates discrimination between induction of DNA damage, oxidative stress and protein misfolding using a novel multicell GFP reporter assay

Giel Hendriks1, Remco S. Derr1, Harry Vrieling2, Robin T. Clark3 1Toxys B.V., Robert Boyleweg 4, 2333 CG, Leiden, The Netherlands 2Department of Human Genetics, Leiden University Medical Center, PO Box 9600, 2300 RC, Leiden, The Netherlands 3MilliporeSigma, a business of Merck KGaA, Darmstadt, Germa...

Live Cell Imaging Reagents

Bring your experiments to life with live cell analysis. Analyzing cellular events in real time can lead to new biological discoveries once unachievable using traditional cellular analysis techniques. Historically, techniques such as PCR, flow cytometry and antibody immunocytochemistry staining were ...

Biomarkers for Neuroscience Research

Understanding and treating neurological diseases is a major challenge due to the complexity and dynamic environment of the central nervous system (CNS). Fortunately, with the use of neuroscience biomarkers these complexities start to unfold and come to life. Biomarkers are essential indicators of ne...

Differentiation and Identification Media for Salmonella

Product Manager Microbiology…. ivo.siegrist@sial.com Salmonella contamination is the second leading cause of food-borne illness worldwide. Controlling outbreaks of Salmonella is an important task for food regulators, restaurants and the food industry in general. The Salmonella family includes over 2...

Method vs. Target Matrix | Detection

Cross Reference Guide This table shows you for which methodology certain types of fluorescent probes, indicators, stains, labels, conjugates, and reagents are used. You will find detailed product lists for these product groups in the corresponding Methodologies section by clicking on the "X" links. ...

CoMoCAT® Single-wall Carbon Nanotubes

Related Products • Carbon Nanomaterials • Graphene Technologies • Nanomaterials Resources Related Articles • Carbon Nanomaterials: Elemental Analyses and Quantification of Their Accumulation in Living Cells • Boron Nitride Nanotubes: Properties, Synthesis and Applications • Novel Properties and Appl...

Raw Material Myths: Separating Fact from Fiction

Douglas Bowman, Program Manager, PharmaGrade at SAFC Myth: All Raw Materials Must Meet GMP Standards Myth: Raw Materials From China Should Always Be Avoided Myth: Chemicals from Western Industrial Manufacturers Are By Default Fit for Purpose Myth: It Is Essential to Source Directly From the Original...

Preservation of moisture sensitive reagents

ChemFiles Vol. 3, No. 1 Strong and Hindered Bases in Organic Synthesis Introduction / Phosphazene Bases / Verkade's Superbases / Traditional bases Preservation of Reagents / Schlenk-Type Glassware Preservation of Moisture Sensitive Reagents and Molarity Determinations To help preserve reagent qualit...

Working with BRAND® Liquid Handling Instruments

The ever increasing demands on the quality of analytical results, and the growing number of samples to be processed result in a need for volumetric instruments which help to handle the routine work in sample preparation as efficiently as possible. Manufacturers of laboratory equipment have responded...

RNA Immunoprecipitation (RIP) FAQs

1. How do you maximize RNA quality during RNA-IP procedure with cross-link protocol? Formaldehyde can compromise DNA and RNA quality and thus the cross-linking time should be optimized to stabilize the RNA-Protein complex without damaging RNA too much. The cross-linking time can range from 2 minutes...

Bioanalytical Products for Isolation, Purification, Sample Prep & Analysis

Sample Preparation and LC-MS Analysis of Small Molecules in Biological Matrices The efficient removal of interfering matrix components followed by fast and accurate quantitation of analytes has become increasingly important in bioanalysis. We provide innovative tools and proven consumables to overco...