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38 matches found for kapa

Kapa Biosystems PCR Reagents & Kits

Researchers require PCR reagents that reliably perform when they need them. Kapa Biosystems PCR reagents are engineered using a method known as directed evolution for optimal performance in standard PCR, qPCR and qRT-PCR applications. Whether you need Kapa Biosystems PCR products for DNA extraction ...

KAPA PROBE FAST qPCR Kits FAQs

What are the recommended applications for KAPA PROBE FAST qPCR Master Mix (2X) Kits? • Gene expression analysis • SNP genotyping/allelic discrimination • Microarray validation • NGS validation What is the enzyme in KAPA PROBE FAST qPCR Master Mix (2X) Kits? This product contains KAPA Taq HotStart DN...

KAPA Express Extract Kit FAQs

For which applications should I use the KAPA Express Extract Kit? The KAPA Express Extract Kit is ideally suited for the extraction of DNA from crude samples for most PCR applications, including qPCR analysis; however, residual inhibitors may result in a quenching of commonly used fluorophores durin...

KAPA PROBE FORCE qPCR Kits

Related Products • Kapa Biosystems High Performance Reagents What are the recommended applications for KAPA PROBE FORCE? • Analysis of challenging sample types: crude DNA extractions from blood, tissue, plant material and environmental samples, cDNA templates/direct carry-over from cDNA-synthesis re...

KAPA SYBR® FAST One-Step qRT-PCR Kits FAQs

What are the recommended applications for KAPA SYBR® FAST One-Step qRT-PCR Kits? • Gene expression analysis • Microarray validation • Gene knockdown validation • RNAi and miRNA research What is the difference between a one-step and a two-step qRT-PCR reaction? In a one-step qRT-PCR reaction, reverse...

KAPA Mouse Genotyping Kit FAQs

What are the recommended applications for the KAPA Mouse Genotyping Kit? KAPA Mouse Genotyping Kits are ideally suited for the rapid extraction and amplification of DNA from mouse tail, ear and toe tissue. The kits are also suitable for the extraction and amplification of DNA from other animal tissu...

Kapa Genomics Reagents - FAQs

What products does the agreement cover? The exclusive global distribution agreement covers Kapa’s qPCR and endpoint PCR portfolios. These products complement our broad life science offering and allow customers to take advantage of our strong eCommerce and logistics capabilities. Products covered by ...

Kapa Biosystems qPCR Reagents and Kits

Quantitative PCR (qPCR) and qRT-PCR reactions require reagents that are accurate and reliable. Kapa Biosystems qPCR and qRT-PCR reagents and kits are designed to provide consistent results for even the most challenging of templates and starting materials. Explore our Kapa Biosystems qPCR reagents an...

KAPA Long Range PCR Kits FAQs

For which applications should I use KAPA Long Range PCR Kits? • PCR amplification of long targets and/or PCR using low amounts of template DNA. • Standard short- and mid-range PCR amplification. • Amplification of PCR products to be used for ligation into 3′-T-overhang cloning vectors. What is the e...

Kapa Biosystems Taq DNA Polymerase and PCR Kits

DNA extraction and amplification are critical steps in your molecular biology workflow and often requires purified, high-quality DNA. Kapa Biosystems offers a robust portfolio of DNA polymerase reagents and PCR kits for extracting and amplifying a variety of mammalian and plant-based genetic materia...

KAPA SYBR® FAST qPCR Kits FAQs

What are the recommended applications for KAPA SYBR® FAST qPCR Master Mix (2X) Kits? Gene expression analysis • Microarray validation • Low-copy gene detection • Gene knockdown validation • ChIP • Next-generation sequencing library quantification What is the enzyme in the KAPA SYBR® FAST qPCR Master...

Rapid SYBR® Green qPCR on the Illumina Eco™ Real-Time PCR System

Related Content • Kapa Genomics Reagents • Roche Biochemical Reagents Fast real-time PCR is a demanding application that requires consistent and reproducible results from the difficult amplicons and low-volume reactions. The combination of the Illumina Eco™ Real-Time PCR System and KAPA SYBR® FAST q...

Directed Evolution

Related Products • Kapa Biosystems Reagents • Roche Biochemical Reagents Related Articles • Rapid SYBR® Green qPCR on the Illumina Eco™ Real-Time PCR System • High-throughput Real-Time PCR: SYBR® Green • Multiplex Real-Time PCR • Direct and Crude Extract qPCR • Optimizing Crude Sample Plant PCR Rela...

High-throughput Real-Time PCR: SYBR® Green

Related Content • Kapa Genomics Reagents • Roche Biochemical Reagents High-throughput qPCR using SYBR® Green is a demanding application that requires consistent and reproducible results from low-volume, high-speed assays. The combination of the Eppendorf Mastercycler® ep realplex4 S real-time PCR in...

Direct and Crude Extract qPCR

Rapid qPCR analysis from highly inhibited tissue and blood sample extractions with KAPA PROBE FORCE qPCR Kits and KAPA Express Extract Enzymatic inhibition, caused by both sample and extraction carryover inhibitors, is a significant obstacle when optimizing crude sample workflows. KAPA PROBE FORCE q...

Optimizing Crude Sample Plant PCR

Overcoming potent inhibitors and sample diversity in direct plant PCR Success rates with direct PCR are typically low due to the high concentrations of inhibitors present in even small amounts of plant material, and the precise control of sample size required. In this Application Note, a systematic ...

Roche Quote

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Multiplex Real-Time PCR

Multiplex qPCR employing probe-based chemistries is a demanding application that often requires extensive optimization and validation. The combination of the multi-channel Eppendorf® Mastercycler® ep realplex4 S realtime PCR cycler and KAPA PROBE FAST qPCR Kits from Kapa Biosystems provides an indus...

Roche Distribution Agreement Q&A

Roche Biochemical Reagents On July 1, 2015 we signed an exclusive, global distribution agreement for Roche Biochemical Reagents. This agreement was then renewed for another five years in 2019. Products covered by the agreement include: • Genomics - Products for DNA labeling and detection, restrictio...

COVID-19新型冠状病毒检测、特征鉴定、疫苗和治疗制剂生产

作为生命科学全球供应商,我们随时准备帮助应对COVID-19疫情。从用于检测、特征鉴定的原材料及科研产品的供应到生产,相关产品和服务已经汇总到一个页面中便于查找和参考。 目前,COVID-19的传播及其对公共健康的影响不断发酵。在这段特殊时期里,我们致力于为研究人员、开发人员和制造商提供必要的产品和服务,以帮助他们应对COVID-19。因此,我们的首要任务仍然是确保员工的安全和福利、满足客户的需求、及时提供准确的信息并确保业务连续性。 我们已经动员了一支全球专题工作小组,积极评估我们的产品和主要原材料供应商的整体供应链,以尽量避免任何潜在的供应中断。利用业务连续性计划,我们继续努力为所有市场的...

Roche Products and Biochemical Reagents

Whether working within a competitive funding landscape or tasked with hitting key business milestones towards financial deliverables, research scientists are also presented with more scientific information and challenges than ever before. As a result, researchers require reliable reagents that are a...

PCR Selection Guide

We offer a wide variety of PCR enzymes, master mixes, and PCR protocols to meet your experimental needs for routine PCR, qPCR, or RT-PCR. Our PCR Selection Guide features various filters to sort by, including the desired application and format. In addition to routine PCR reagents, we also provide a ...

KAPA2G Robust PCR Kits FAQs

What are the recommended applications for KAPA2G Robust PCR Kits? • Amplification of templates with high GC or AT content • Amplification of templates containing common PCR inhibitors at levels inhibitory to wild-type Taq • Amplification from crude samples, e.g. buccal swabs; cultured mammalian, bac...

Characterisation of Antibodies with BRAND® PCR Plates

Introduction The generation of fully human antibodies with unique functional properties that are exploitable for tailored therapeutic interventions remains a major challenge in the antibody technology field. However, it is possible to isolate these antibodies from human B-cellls in contact with spec...

RT-PCR | Reverse Transcription PCR

Download printable RT PCR diagram On This Page • What Is RT-PCR? • RT-PCR Kits • cDNA Synthesis Kits & Mixes • RT Enzymes – Reverse Transcriptases • Additional PCR Reagents Reverse transcription polymerase chain reaction (RT-PCR) is a variation of standard PCR that involves the amplification of spec...

Коронавирус COVID-19 (SARS-CoV-2): обнаружение, характеризация и производство вакцин

Наша компания является международным поставщиком товаров и услуг в области Life Science и всегда готова помочь Вам в борьбе с COVID-19. Мы объединили все необходимые товары и услуги на одной странице: от исходных материалов и продуктов для обнаружения и характеризации вируса до услуг по производству...

RT-qPCR – Quantitative Reverse Transcription PCR

On This Page • What Is RT-PCR? • Applications of RT-qPCR • One-Step RT-qPCR vs Two-Step RT-qPCR • One-Step RT-qPCR Products for High-Throughput Applications • Two-Step RT-qPCR Products for Higher Flexibility • qPCR Kits • MicroRNA-Based RT-qPCR • qPCR Assay Designs and Reagents for SARS-CoV-2 Resear...

Coronavirus COVID-19 (SARS-CoV-2) – Nachweis, Charakterisierung, Impfstoff- und Therapeutikaherstellung

Als ein globaler Anbieter im Bereich Life-Science unterstützen wir Ihre Reaktion auf COVID-19. Wir haben alle hierfür relevanten Produkte auf einer einzigen Seite zusammengefasst: Von Rohstoffen und Produkten für die Forschung, für den Nachweis und die Charakterisierung bis hin zur Impfstoffherstell...

Coronavirus qPCR Design Case Study & Related Products to Support SARS-CoV-2 Research

Letter to Our Customers • SARS-CoV-2 qPCR Assay Design & Reactivity • SARS vs. SARS-CoV-2 Positive Control Assay • Order SARS-CoV-2 Primers and Probes • RT-PCR and RT-qPCR Genetic Analysis and Supporting Reagents We are working hand in hand with public health authorities and customers to support the...

KAPA3G Plant PCR Kits FAQs

What are the recommended applications for KAPA3G Plant PCR Kits? • Direct PCR from crude plant samples including leaf tissue and seed • Crude PCR from leaf tissue and seeds • Successful amplification of CTAB-extracted DNA • Streamlined workflows for transgenic screening • As a solution for improving...