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986 matches found for mass_spectrometry

Alternate Selectivity in HPLC 5: Phenyl Polar Phases [VIDEO]

This 43-minute Supelco web seminar will describe the chemical structure of various types of bonded phases. Some possible explanations why polar RP phases have different selectivity from nonpolar straight-chain alkyl phases will be proposed, and classes of compounds that are best separated by polar R...

Carbopack™ X TD Tube for Fenceline Monitoring (EPA Method 325B)

EPA Method 325B Compliance Overview The US EPA first employed Supelco's adsorbent technology beginning in 1981 with the development of the first thermal desorption tubes as an alternative to sampling volatile organic compounds with bulky canisters and less sensitive solvent desorption methods. When ...

A Comparison of Peptides to Whole-Protein Stable Isotope Labeled Internal Standards in Quantitative Protein MS Workflows

Characterize a stable isotope labeled human erythropoietin (SILu™Prot EPO) and compare it’s use as an internal standard in an immunoaffinity enrichment experiment to the SIL peptide approach. LC-MS/MS methods employing the sensitivity of multiple reaction monitoring (MRM) for quantification of prote...

Water for Clinical and Biomedical Laboratories

Feeding clinical chemistry and biochemistry analyzers Water purification systems are set-up to direct-feed the analyzers. To support laboratories in their choice of a water purification solution, water quality recommendations have been proposed by the CLSI, in the C3-A4 guideline “Preparation and te...

Sweet Poison - TraceCERT® CRM Solution for Tutin

Tutin is a toxic oxygenated sesquiterpene occurring in the tutu plant (genus coriaria) that is found in New Zealand. It is responsible for poisoning through contaminated honey, when honeybees collect honeydew produced by sap-sucking vine hopper insects that feed on the tutu plant. Therefore, the Aus...

Environmental Testing - Webinars

Take advantage of our expertise in various topics and techniques related to environmental testing. • Pesticides – From analytical standards to certified reference materials Certified reference materials (CRMs) are an essential part of quality assurance. Laboratories accredited according to ISO 17025...

Purification of Serum Peptides for MALDI Mass Spectrometry

Biomarkers play an essential role in the drug discovery and development process. They provide powerful clues to genetic susceptibility, disease progression and drug response. Serum is a key source of putative protein biomarkers. One major impediment to discovering new biomarkers is the presence of s...

Alternate Selectivity in HPLC 2: Polar Alternatives to C18 or C8 [VIDEO]

This 43-minute Supelco web seminar will describe the chemical structure of various types of bonded phases. Some possible explanations why polar RP phases have different selectivity from nonpolar straight-chain alkyl phases will be proposed, and classes of compounds that are best separated by polar R...

Dry Powder Media Global Supply

SAFC Overview Why Choose SAFC? Media Supply Strategy DPM Manufacturing Capability and Capacity Packaging and Logistics Global Quality Systems Raw Materials Scale-up & Support Services Small Volume Custom Media – imMEDIAte Advantage™ Erythropoietin Hormone (Molecular model) Erythropoietin (EPO) is a ...

Scorpions® Probes

Scorpions probes are highly sensitive, sequence-specific, bi-labeled fluorescent probe/primer hybrids designed for qPCR. • SNP detection • Allele discrimination • Pathogen detection • Multiplexing • Viral load quantification • Gene expression analysis • Gene copy determination • Endpoint genotyping ...

Liquid Chromatography & HPLC, Supelco Reporter 29.5

Serum Lipidomics Profiling using an Ascentis Express Fused-Core C18 Column HPLC Method for the Separation of Nine Key Components of Milk Thistle Serum Lipidomics Profiling using an Ascentis Express Fused-Core C18 Column back to top The following was generated with the assistance of an outside source...

Proteoprep Kits

Product Name Product # Function Samples Processed Input Sample Size Output Compatibility ProteoPrep® Universal Extraction Kit PROT-TWO Isolate two fractions from cells enriching proteins based on solubility profile 10 2 mL Two-dimensional electrophoresis (2DE) ProteoPrep® Membrane Extraction Kit PRO...

LC×LC/MS Method for Identification of Triacylglycerols in Marine Organisms using Different Extraction Procedures

The sea and its living organisms constitute samples of high interest in environmental analysis. Knowledge of the chemistry of the sea and marine species can provide reliable information about pollution (through direct analysis of the water or measurement of bioaccumulated contaminants), nutritional ...

Pure and Simple Primers | Custom DNA Oligos

Order Pure and Simple Primers, and you will be assured a quality start to all of your research applications with our ultra-high base coupling efficiency and optimized cartridge purification process. Guaranteed Yield 3 OD Format Shipped dry in 2 mL screw cap tubes Length 12 - 35 bases Purification Re...

Prestige Antibodies — Monospecific Antibodies Designed for Immunohistochemical Analysis

• • Download PDF • Well-characterized antibodies are essential tools for protein studies, global proteomics analysis, as well as for clinical diagnostics. Although production of antibodies is a well-established process and a large number of antibodies are commercially available through many vendors,...

High Purity Metalorganic Precursors for CPV Device Fabrication

• • Download PDF • SAFC Hitech Limited, Sigma-Aldrich Corporation Email: ravi.kanjolia@sial.com Thin film photovoltaic devices have become increasingly important in efficiently harnessing solar energy to meet consumer demand. Conventional crystalline silicon solar cells have demonstrated remarkably ...

Cold Spring Harbor Laboratory Protocols

We have prepared product lists for commonly used protocols from Cold Spring Harbor Protocols. The product lists correspond to protocols for the following topics: • Antibodies • RNAi • Genomics • Neuroscience • Proteomics • Stem Cell Biology • Removal of Cross-reactive Antibodies from Antiserum: Affi...

TruPAGE™ Precast Gels FAQ

• • Download PDF • Do I have to buy a new tank in order to use TruPAGE Gels? No. TruPAGE cassettes come in 10×10 cm and 10×8 cm which are compatible with most popular gel tanks. However, if you would like a new tank, Sigma-Aldrich recommends using out RunBlue™ Dual Run and Blot System (Catalog Numbe...

SOLVO® Vesicular Transporter Assays

Membrane preparations for vesicular transport assays are suitable for general drug-efflux transporter interaction studies. Both substrate and inhibitor interactions can be assessed using membrane vesicles prepared from mammalian cell lines over-expressing key human transporters. Human efflux transpo...

Hopane Reference Standard Biomarkers from Sigma-Aldrich

An interesting application of analytical chemistry is in biomarker analysis: the use of a chemical, a set of chemicals, or chemical modifications, as clues to answering scientific questions. Sigma-Aldrich® researchers and product managers pay close attention to the needs in the biomarker industry, p...

Solid Phase Microextraction (SPME) Method for 4-methyl-1-cyclohexanemethanol (MCHM) and propylene glycol phenyl ether (PPH) in Water

Background 4-Methyl cyclohexane methanol (MCHM) and propylene glycol phenyl ether (PPH) are chemicals used in the coal cleaning process. On January 9, 2014, there was an accidental release into the Elk River in West Virginia of an estimated 10,000 gallons of a mixture of these chemicals[1]. Accordin...

Rely on Sigma for Trusted Mass Spectrometry Essentials

• Trypsin, Proteomics Grade • ProteoMass Kit for MALDI-MS Calibration • MiniTips™ • Universal Proteomics Standard Trypsin, Proteomics Grade Sigma’s family of Proteomics Trypsin products meets all of your proteolytic needs! • For traditional digests, Proteomics Grade Trypsin is highly purified, dimet...

Alternate Selectivity in HPLC 3: Method Development w/Polar Columns [VIDEO]

This 43-minute Supelco web seminar will describe the chemical structure of various types of bonded phases. Some possible explanations why polar RP phases have different selectivity from nonpolar straight-chain alkyl phases will be proposed, and classes of compounds that are best separated by polar R...

LightCycler® Probes

LightCycler probes are highly sensitive and sequence-specific fluorescent probes designed for use with the Roche LightCycler instruments. • SNP detection • Allele discrimination • Pathogen detection • Multiplexing • Viral load quantification • Gene expression analysis • Gene copy determination Sever...

Improved Analysis of Simple Sugars Using apHera™ NH2 HPLC Columns

Hugh M. Cramer and Ric Cone ric.cone@sial.com Chromatographic analysis of simple sugars is important to the food and beverage, pharmaceutical and biotech industries. Effective separations of sugars can be challenging because these compounds are highly polar, uncharged, and lack a chromophore. The pr...

Analytical Chemistry & Chromatography Products

• Air Monitoring • Analytical Reagents • Analytical Standards • Applications • Chiral Chromatography • Chromatography Standards • Derivatization Reagents • Food & Beverage Analysis • Gas Chromatography • HPLC • Ion Sensor Materials • Karl Fischer Titration • Laboratory Supplies • LPLC/TLC/Paper Chro...

Development of Two Multiplex Immunoassays and Preliminary Application in Determining Protein Depletion

Depletion of Highly Abundant Protein (HAP) and Moderately Abundant Protein (MAP) from plasma or serum samples can be achieved through Sigma Seppro Tandem IgY14/ SuperMix columns. A range of 77–129 MAPs has been identified from the bound fraction of Seppro SuperMix resin by LC/MS-MS method. However, ...

Determination of Triclosan in Environmental Waters

Related Articles • Reporter US Volume 32.2 (4 Mb PDF) • Determination of Bisphenol A in Drinking Water • SPME for the Extraction of Pharmaceutical Compounds From Water • SPME Method for 4-methyl-1-cyclohexanemethanol (MCHM) and propylene glycol phenyl ether (PPH) in Water Introduction Triclosan is a...

Paints and Coatings - Air Sampling products from Supelco

Paints consist of mainly three components: (1) a pigment to add color which sometimes contains heavy metals such as lead, chromium and cadmium; (2) a binding agent or “vehicle” which bonds the paint pigment, remains on the coated surface when dry, and also gives paints its protective properties; and...

Ascentis HPLC Application Notebook Index Alpha

Listing by Phase and Title Return to index selection Phase: C18 C18, LC-MS C8 C8, LC-MS/LC-UV RP-Amide RP-Amide, LC-MS/ LC-UV Ascentis C18 back to top Title Application Report No. Acebutolol and Pindolol Antihypertensives Using Ascentis C18, Analysis of 95 Analgesics Using Ascentis C18, Analysis of ...