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303 matches found for microscopy

AD030 Particle Monitoring Guide – Particle Monitoring Selection Guide

This page addresses the characteristics of each membrane as they pertain specifically to particle monitoring. Click on the membrane type for details: • MF-Millipore™ Mixed Cellulose Esters • Isopore™ Track-Etched Polycarbonate • Fluoropore™ and Mitex™ (PTFE) Membranes • Silver Membranes • Durapore® ...

April 2001 Cell Biology

A TECHNICAL APPLICATION NEWSLETTER VOL 2 ISSUE 2 April 2001 Cell Biology Leptomycin B: An Important Tool for the Study of Nuclear Export by Yael Asscher,* Shlomo Pleban,* Marcia Ben-Shushan,* Michal Levin-Khalifa,* Zhong Yao,† and Rony Seger† Sigma-Aldrich Corporation,* Jerusalem, Isra...

Sigma-Aldrich Product Portfolio

Sigma-Aldrich gives you easy access to the product you need • by streamlining our product range • by delivering clearly defined product categories • by providing brands and grades for every application Sigma-Aldrich Brands PRODUCT BRANDS Supelco® Sigma® Sigma-Aldrich® SAFC APPLICATIONS Analytical Ca...

FP-ZFNs or Fluorescent Protein-Linked Zinc Finger Nucleases

Zinc finger nucleases (ZFNs) have enabled highly efficient gene targeting in a wide variety of cell types and organisms. While CompoZr® methods for engineering highly active ZFNs have become robust and reproducible, challenges relating to ZFN delivery and expression continue to limit isolation of co...

Tips and Techniques for Troubleshooting Immunohistochemistry (IHC)

Immunohistochemistry (IHC) is a technique in which antibodies are used to detect an antigen in sectioned tissue and is ubiquitous in the research, preclinical, and clinical settings for visualizing cellular components. This technique, which was first used in the 1940s, is widely used to increase und...

Tech Tips: MISSION® esiRNA | Biowire Winter 2011

Subscribe to Biowire and eBiowire Biowire Winter 2011 — Knockout Rats — More Effective Pre-Clinical Models Table of Contents • Study Spotlights ○ The Unique Features of Prestige Antibodies® ○ The New Human Protein Atlas • Methods ○ microRNA Target Validation MISSION® 3’UTR Lenti GoClone™ and Human m...

Recent Progress in Spintronic Materials

Soubantika Palchoudhury,1 Karthik Ramasamy,2* Arunava Gupta3* 1Civil and Chemical Engineering, University of Tennessee at Chattanooga, Chattanooga, Tennessee 37403, USA 2Center for Integrated Nanotechnologies, Los Alamos National Laboratory, Albuquerque, New Mexico 87185, USA 3Center for Materials f...

Chemical Compatibility Guide for MultiScreen® Filter Plates

Ratings are based on 100% or concentrated solutions, unless otherwise indicated. Aromatic hydrocarbons (especially ketones, DMF, DMAC, DMSO, THF, acetonitrile) and chlorinated hydrocarbons will attack both membranes and plastics. A chemical listed as NR may be suitable in low concentrations for shor...

Tubulin

Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton. Tubulin is a ...

actin-related protein T2
Synonyms ARPM2, ARPT2, Arp-T2, HARPM2
Species Human (140625) , Species Mouse (73353) , Species Rat (298671) , Species chicken (428178) , Species cow (515214) , Species domestic cat (101087248) , Species dog (479581) , Species domestic guinea pig (100724608) , Species naked mole-rat (101722284) , Species sheep (101102916)
Summary: The protein encoded by this intronless gene belongs to the actin family. Studies have shown that this protein may be involved in cytoskeletal organization similar to other cytoplasmic actin-related protein (ARP) subfamily members. Antibody raised against the human protein has been used to detect the protein by immunoblotting and immunofluorescence microscopy, demonstrating its specific synthesis in the testis, late in spermatid differentiation, and its localization in the calyx. [provided by RefSeq, Jul 2008]

Nanomaterials for Energy Storage in Lithium-ion Battery Applications

• • Download PDF • Ganesh Venugopal Andrew Hunt Faisal Alamgir Ganesh Venugopal1, Andrew Hunt1, and Faisal Alamgir2 1nGimat Co., 5315 Peachtree Blvd., Atlanta, GA, USA 2Georgia Institute of Technology, Department of Materials Science and Engineering 771 Ferst Drive, N.W., Atlanta, GA 30332 Email: gv...

Proximity Ligation Assay Seminar at UW-Madison

Seminar Title: Proximity Ligation Assay: Sensitive Protein Detection for Oncology Research Date: Friday, November 3, 2017 Time: 10:00 AM – 11:30 AM Check-In: 9:45 AM Seminar: 10:00 AM – 11:00 AM Q&A: 11:00 AM – 11:30 AM Refreshments will be provided by MilliporeSigma for attendees. Location: UW-Madi...

Baseclick EdU Cell Proliferation Detection Kits: Improve Data Quality and Save Time

Experimental studies measuring cell proliferation have had implications in cancer biology, immunology, cell biology, and developmental biology. Various methods for monitoring cell proliferation have been developed. Counting cells using a hemocytometer is one of the simplest methods used to monitor c...

The Power of Duolink® flowPLA Detection Kits in Studying Protein Interactions, Post-Translational Modifications, and Low-Abundant Protein Targets

Duolink® PLA technology has been used by researchers to study protein events via microscopy and more recently in flow cytometry experiments. This article describes the new possibilities that are available to flow cytometry users to study protein-protein interactions, post-translational modifications...

Testing Service for radiello® Diffusive Samplers

Looking for analytical testing services? Below is a list of test labs we recommend for radiello products. Please contact these labs directly; their services are not available through Sigma-Aldrich. Europe Istituti Clinici Scientifici Maugeri (ICSM) IPROMA laboratorio y asesoria TERA Environnement La...

LuminiCell Live Cell Tracking Nanoparticles

Related Products • CellASIC Live Cell Imaging • Live Cell Imaging Reagents • Fluorescent Dyes and Probes • Lentiviral Biosensors • Live Cell Imaging Videos • Cell Culture Media • Cell Lines • Cellular Assays • Material Sciences • Nanomaterials • Antibodies Fluorescent imaging techniques with high sp...

Chemical Synthesis of Monodisperse Magnetic Nanoparticles

• • Download PDF • Sheng Peng and Shouheng Sun* Department of Chemistry Brown University Providence, Rhode Island 02912, USA *E-mail: ssun@brown.edu Magnetic nanoparticles have attracted tremendous attention due to their novel properties and their potential applications in magnetic recording, magnet...

New Cancer Biomarker | BioFiles Volume 5 Number 6 - Cancer

BioFiles Volume 5, Number 6 — Cancer Download BioFiles v5 n6 (3.38 Mb PDF) Table of Contents • Introduction • New Cancer Biomarker Breast Cancer Malignant Melanoma Epithelial Ovarian Cancer Colon Cancer Summary • Cancer Research: Epigentic Mechanisms • Folic Acid Metabolism: A Role in Cancer's Cause...

Conductive Polymers for Advanced Micro- and Nano-fabrication Processes

• • Download PDF • 1 Optoelectronics Research Group, School of Engineering, University of Glasgow, Rankine Building, Oakfield Avenue, Glasgow G12 8LT, United Kingdom 2 Bioelectronics Research Centre, School of Engineering, University of Glasgow, United Kingdom *Email: Faiz.Rahman@glasgow.ac.uk Condu...

Analytical Reagents Explorer Lookup

» Standards » Microbiology » Applications Use the search box below to quickly find the analytical reagent, buffer, or solvent you need from the selection of a few thousand products in our web catalog. Search Instructions : • Use any one of the four available search parameter fields. • Click the 'Sea...

Amnis Imaging Flow Cytometry: What can imaging in flow do for you?

Seminar Title: Amnis Imaging Flow Cytometry: What can imaging in flow do for you? Date: Thursday, May 17, 2018 Time: Check In: 1:45 PM Seminar: 2:00 PM- 3:00 PM Refreshments will be provided. Location: Northwestern University Gray Seminar Room Sponsored By: Flow Cytometry Core The Amnis cytometry sy...

Removal of Percoll after centrifugation

Extracted from Cell Separation Media Methodology and Applications, GE Healthcare, 2007 Since Percoll is non-toxic to biological materials and does not adhere to membranes, it is usually unnecessary to remove Percoll from the purified preparation. Cells can be transferred directly to cell culture sys...

Silver Nanomaterials: Properties & Applications

Silver nanomaterials have unique physical, chemical and optical properties that are being leveraged for a wide variety of applications. A resurgence of interest in the utility of silver as a broad based antimicrobial agent has led to the development of hundreds of products that incorporate silver na...

X-ray Crystallography/Crystallization | BioFiles v5 n2 Protein Characterization & Detection

BioFiles Volume 5, Number 2 — Protein Characterization & Detection Download Biofiles v5 n2 (3.4 Mb PDF) Table of Contents • Introduction • Labeling of Proteins • X-ray Crystallography/Crystallization • MALDI Mass Spectrometry • Colorimetric Electrophoresis Stains • IEF Focusing • Western Blotting • ...

Duolink® PLA Flow Cytometry Protocol

This protocol describes the use of Duolink® PLA reagents for the detection of individual proteins, protein modifications, and protein-protein interactions within cell populations by flow cytometry. Duolink® PLA Reagents To run a Duolink® PLA experiment for detection by flow cytometry, the following ...

FITC-labelled polysaccharides

FITC-Dextran FITC-DEAE-dextran (FDD) FITC-carboxymethyl-dextran (FCM-Dextran) FITC-polysucrose (FITC-Ficoll®) FITC-DEAE-polysucrose FITC-CM-polysucrose (FITC-CM-Ficoll®) FITC-inulin Fluorescein hyaluronic acid (FHA-Se) TRITC-labelled polysaccharides TRITC-polysucrose (TRITC-Ficoll®) Tetramethyl-rhod...

NIR Fluorescence Dyes

• • Download PDF • An increasing demand for red emitting fluorescent dyes, useful in life science applications such as antibody and protein labeling, prompted us to develop new improved long wavelength markers for use in the visible/near-infrared (NIR) region. Tracy 645 and Tracy 652 (see Table 1) a...

Flow Cytometry and Microscopy Seminar

Seminar Title: Detection of Protein-protein Interactions, Post-translational Modifications, and Multiple Protein Events by Flow Cytometry and Microscopy Date: Wednesday, August 8, 2018 Time: Lecture 10:30 AM – 11:30 AM Check-in: 10:15 AM Location: Northwestern University Chicago Campus Lurie Buildin...

Preparation & Staining of 3D Printed Tissues & Scaffolds

Three-dimensional (3D) printing of biological tissue is rapidly becoming an integral part of tissue engineering. With advances in 3D printing technology, numerous novel fabrication methods are now possible, allowing for the production of cell laden constructs,1-2 composite tissue2 as well as scaffol...

Early Detection of Fungal Hyphae on Bread by Fluorescently Labelled Lectins

Monika Baeumle, Product Manager Biochemistry monika.baeumle@sial.com Bernhard Schoenenberger, Supervisor R&D; Jakob Zbaeren, Inselspital Bern, Switzerland Food quality assurance and control have gained importance as factors of concern to public health. Perishable foods are of particular focus for ro...

Co-culture of Tumor and Stromal Cells in TrueGel3D™ Hydrogels

Related Links • 3D cell culture • 3D hydrogels • TrueGel3D™ hydrogels • Biomaterials Related Products • Cell culture • 3D cell culture • ECM proteins • 3D Scaffolds • 3D hydrogels Tumor microenvironment (TME) is a complex aggregation of malignant cells and non-transformed cells (fibroblasts, neuroen...