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    Search term:"microscopy"

    284 matches found for microscopy

    Hematopoietic Stem Cells Protocols

    Preparation of Mouse Embryonic Fibroblast (MEF) Feeder Cell Layer Culture of hES Cells Induction of hES Cell Differentiation Expansion of hES-blast Colony (BC) Cells Preparation of Mouse Embryonic Fibroblast (MEF) Feeder Cell Layer • Eviscerate 12.5 dpc ICR or CD-1 mouse embryos leaving the heads on...

    Abberior® Dyes

    For Superior Super-Resolution Microscopy Application Benefits • Optimized for brightness and very low background • Optimized switching behavior being the key for super-resolution • All markers are tested for different super-resolution methods ○ Abberior STAR for STED, confocal and epifluorescence im...

    Duolink® PLA Flow Cytometry Kits

    Traditional flow cytometry is limited to identifying co-expression levels, or co-localization in the case of flow imaging, rendering any protein-protein interaction by flow cytometry impossible — until now. Duolink® Proximity Ligation Assay (PLA) provides an amplification step that yields greater se...

    Elispot assays: State-of-the-art tools for functional analysis of cellular immunology

    Abstract Under optimal conditions, the enzyme-linked immunosorbent spot (Elispot) assay enables visualization of multiple secretory products from a single responding cell. Thus, the Elispot provides both qualitative (type of immune protein) and quantitative (number of responding cells) information. ...

    Nanomaterials for Energy Storage in Lithium-ion Battery Applications

    • • Download PDF • Ganesh Venugopal Andrew Hunt Faisal Alamgir Ganesh Venugopal1, Andrew Hunt1, and Faisal Alamgir2 1nGimat Co., 5315 Peachtree Blvd., Atlanta, GA, USA 2Georgia Institute of Technology, Department of Materials Science and Engineering 771 Ferst Drive, N.W., Atlanta, GA 30332 Email: gv...

    Prestige Antibodies® on The Cell Atlas

    In 2015, the Human Protein Atlas project presented a complete map of the human proteome expression in human tissues. The Tissue Atlas and Cancer Atlas includes data for 17,500 Prestige Antibodies. The newest addition to the Human Protein Atlas is the Cell Atlas - a fantastic tool for anyone working ...

    Co-culture of Tumor and Stromal cells

    Related Links • 3D cell culture • 3D hydrogels • TrueGel3D™ hydrogels • Biomaterials Related Products • Cell culture • 3D cell culture • ECM proteins • 3D Scaffolds • 3D hydrogels Tumor microenvironment (TME) is a complex aggregation of malignant cells and non-transformed cells (fibroblasts, neuroen...

    Perfluorosulfonic Acid Membranes for Fuel Cell and Electrolyser Applications

    Deborah Jones Institut Charles Gerhardt CNRS–University of Montpellier, 34090 Montpellier, France Email: Deborah.Jones@univ-montp2.fr Introduction PFSA Polymer Types Composition, Structure, and Synthesis Routes PFSA Ionomer Dispersions Conductivity and Water Uptake Membrane Durability Better Membran...

    Cell Signaling Assays Kit Platforms

    • Cell Fractionation • Dot Blot • EIA • ELISA • Enzymatic • Flow Cytometry and Fluorescence • Histochemical • Immunoprecipitation • Radioactive Cell Fractionation Various techniques designed to separate cell components into subcellular fractions that preserve the structure and physiology of its func...

    actin-related protein T2
    Synonyms ARPM2, ARPT2, Arp-T2, HARPM2
    Species Human (140625) , Species Mouse (73353) , Species Rat (298671) , Species chicken (428178) , Species cow (515214) , Species domestic cat (101087248) , Species dog (479581) , Species domestic guinea pig (100724608) , Species naked mole-rat (101722284) , Species sheep (101102916)
    Summary: The protein encoded by this intronless gene belongs to the actin family. Studies have shown that this protein may be involved in cytoskeletal organization similar to other cytoplasmic actin-related protein (ARP) subfamily members. Antibody raised against the human protein has been used to detect the protein by immunoblotting and immunofluorescence microscopy, demonstrating its specific synthesis in the testis, late in spermatid differentiation, and its localization in the calyx. [provided by RefSeq, Jul 2008]

    Silver Nanomaterials: Properties & Applications

    Silver nanomaterials have unique physical, chemical and optical properties that are being leveraged for a wide variety of applications. A resurgence of interest in the utility of silver as a broad based antimicrobial agent has led to the development of hundreds of products that incorporate silver na...

    Immunohistochemistry: Current Applications in Skin Cancer

    Cutaneous Spindle Cell Tumors Cutaneous Adnexal Neoplasms Melanocytic Neoplasms Malignant Small Blue Cell Tumors Fibrohistiocytic Lesions Vascular Neoplasms Hematopoietic and Hystiocytic Tumors Infectious Agents Although immunohistochemistry techniques and applications have made great strides in the...

    Fluorescence Lifetime Measurement Advantages & Applications

    Fluorescence lifetime (FLT) is the time a fluorophore spends in the excited state before emitting a photon and returning to the ground state. FLT can vary from picoseconds to hundreds of nanoseconds depending on the fluorophore. The lifetime of a population of fluorophores is the time measured for t...

    Biodegradable Polyester Based Nanoparticle Formation by Miniemulsion Technique

    Aliphatic polyesters such as polylactide, poly(lactide-co-glycolide) and polycaprolactone, as well as their copolymers, represent a diverse family of synthetic biodegradable polymers that have been widely explored for medical uses and are commercially available.1 These polyesters have excellent tiss...

    Tips for Cell Engineering using Cas9-GFP CRISPR plasmids

    Related Article Introduction to CRISPR A CRISPR/Cas-GFP Vector for Rapid Expression Verification View CRISPR Webinar CRISPR endonucleases have shown wide variation in their activity, even among multiple CRISPRs designed within close genomic proximity.1 For this reason, we highly recommend that you t...

    April 2001 Cell Biology

    A TECHNICAL APPLICATION NEWSLETTER VOL 2 ISSUE 2 April 2001 Cell Biology Leptomycin B: An Important Tool for the Study of Nuclear Export by Yael Asscher,* Shlomo Pleban,* Marcia Ben-Shushan,* Michal Levin-Khalifa,* Zhong Yao,† and Rony Seger† Sigma-Aldrich Corporation,* Jerusalem, Isra...

    Organelle Isolation | BioFiles Volume 6, Number 5 — Centrifugation

    Download BioFiles v6 n5 (4.66 Mb PDF) The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, and density, centrifugation can be easily employed to separate a...

    Lentiviral Production Using X-tremeGENE HP Transfection Reagent

    Lentiviruses represent a powerful tool in research applications to transduce a wide range of cell types. During virus production, low titers and toxicity can be challenging. This easy-to-use protocol uses X-tremeGENE HP Transfection Reagent for fast and robust lentiviral preparation, with high virus...

    3D Neural Stem Cell Models of Alzheimer’s Disease Protocol

    Introduction Protocol • Human ReNcell® VM NSC Maintenance and Passaging • Viral Infection of Human ReNcell® VM NSCs • Enrichment of High-Expressing Transgenic Human ReNcell® VM NSCs • Culturing Sorted Human ReNcell® VM NSCs • 3D Matrix Cultures of Human ReNcell® VM NSCs • 3D Culture Cell Seeding of ...

    Tubulin

    Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton. Tubulin is a ...

    Duolink® Seminar at UCHC

    Seminar Title: Duolink® PLA Technology - Powerful Tool for Study of Endogenous Protein Function Date: Wednesday, February 28, 2018 Time: 12:00 PM – 1:30 PM Check In & Lunch: 12:00 – 12:15 PM Seminar: 12:15 PM – 1:00 PM Q&A: 1:00 PM – 1:30 PM Location: Engineering and Science Building Main Floor Lobb...

    Identification of Mycobacteria

    • • Download PDF • PhD, Institute of Tropical Medicine, Mycobacteriology Unit, Belgium …. amartin@itg.be Figure 1 Typical colony of Mycobacterium tuberculosis seen under a microscope with 10x magnification The diseases produced by species of the genus Mycobacterium are important causes of morbidity ...

    Read the latest developments in CRISPR research

    read abstract Targeted activation of diverse CRISPR-Cas systems for mammalian genome editing via proximal CRISPR targeting. Chen Fuqiang, Ding Xiao, Feng Yongmei, Seebeck Timothy, Jiang Yanfang, Davis GregoryD Nature Communications 2017-04-01 Bacterial CRISPR-Cas systems comprise diverse effector en...

    Immunohistochemistry: Current Applications in Gastrointestinal Cancer

    Incidence Deaths Lifetime risk of Colorectal Cancer Signs and Symptoms Immunohistochemistry and Colon Cancer Metastatic Carcinoma [Webinar] Colorectal Cancer and Immunohistochemistry Materials References Colorectal cancer is the third most common cancer in both men and women. An estimated 136,000 ca...

    Nanostructured Olivine-based Cathode Materials for Lithium-ion Batteries

    Vishwanathan Ramar and Palani Balaya* Department of Mechanical Engineering National University of Singapore, Singapore-117576 *Email: mpepb@nus.edu.sg Due to the adverse impact of the continued use of fossil fuels on the earth’s environment and climate, researchers have been asked to develop new app...

    Tips for Optimizing Immunofluorescence Protocols to Get the Best Image

    Related Links • Antibody Explorer: Search primary and secondary antibodies • Prestige Antibodies • Antibody Resource Center Uses for immunofluorescence (IF)—where an antibody is conjugated to a molecule that fluoresces when excited by lasers— include protein localization, confirmation of post-transl...

    Detection Learning Center

    • Ampliflu™ Red Peroxidase Substrate • Atto Dyes • Conjugates • LUCY® Stains • Method-to-Product Guide • Nancy-520 • Spectral Fluorescence Standard Kit • Super-resolution Microscopy - Nanoscopy • Wavelength Index • 94433 β-Galactosidase Stain

    Nanofluids for Biomedical Applications Using Spherical Iron Oxide Magnetic Nanoparticles Fabricated by High-Power Physical Evaporation

    A. P. Safronov,1,2 I. V. Beketov,1,2 O. M. Samatov,1 G. V. Kurlyandskaya,2,3,* S. M. Bhagat,4 A. Larrañaga,5 I. Orue,5 R. Andrade5 1Institute of Electrophysics, RAS, Amundsen St. 106, 620016, Ekaterinburg, Russia 2Ural Federal University, Lenin St. 51, 620000, Ekaterinburg, Russia 3 Department of El...

    FITC-labelled polysaccharides

    FITC-Dextran FITC-DEAE-dextran (FDD) FITC-carboxymethyl-dextran (FCM-Dextran) FITC-polysucrose (FITC-Ficoll®) FITC-DEAE-polysucrose FITC-CM-polysucrose (FITC-CM-Ficoll®) FITC-inulin Fluorescein hyaluronic acid (FHA-Se) TRITC-labelled polysaccharides TRITC-polysucrose (TRITC-Ficoll®) Tetramethyl-rhod...

    Optimal Cell Density Determination for Transduction Using MISSION LentiExpress Optimization Plate

    • • Download PDF • • 0.25% Trypsin EDTA solution • LentiExpress Optimization Plate (Product No. SHXC01) • Puromycin Solution (Product No. P9620) • Complete Media—RPMI 1640 with 10% fetal bovine serum • Polybrene (hexadimethrine bromide) (Product No. H9268) • Electronic repeating pipette • Fluorescen...

    Functional Magnetic Materials: Synergy Between Basic Science and Evolving Technology

    S. B. Roy Materials and Advanced Accelerator Sciences Division Raja Ramanna Centre for Advanced Technology Indore-452013, India Email: sbroy@rrcat.gov.in The application of magnetism and magnetic materials pervades our modern civilization in the form of electrical power, communications and informati...