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95 matches found for mycoplasma

Mycoplasma - Media by Microorganism

Sigma-Aldrich Online Catalog Product List: Mycoplasma

Mycoplasma Detection - Reagents and Supplements

Sigma-Aldrich Online Catalog Product List: Mycoplasma Detection

Mycoplasma PCR ELISA Protocol

Product No. 11663925910 Determination of lysed Mycoplasma Dead and lysed Mycoplasma will have degraded DNA, therefore, it is likely that only live Mycoplasma with intact DNA will be detected. Interupting the Mycoplasma PCR ELISA The Mycoplasma PCR ELISA can be interrupted at the following steps: Aft...

Mycoplasma Detection and Elimination

Related Links • Product List The maintenance of contamination-free cell cultures is fundamental to cell-based research. Among the most pervasive concerns is contamination with mycoplasma, a genus of bacteria whose members are typically sub-micron in size and lacking a cell wall, making them difficul...

PCR-based Detection of Mycoplasma Contamination

The maintenance of contamination-free cell lines is essential to cell-based research. Among the biggest contaminant concerns are mycoplasma contamination. Although mycoplasma do not usually kill contaminated cells, they are difficult to detect and can cause a variety of effects on cultured cells, in...

Cell Culture Protocol 10: Testing Cells for Mycoplasma Contamination by Hoechst DNA Staining

Related Links • Cell Culture Homepage • Fundamental Techniques in Cell Culture- ECACC Handbook • ECACC Cell Lines • Fetal Bovine Serum • Classic Media and Salts • Cell Culture Reagents • Cell Culture Supplements • Stericup® Media Filtration • Primary Cell Culture • Stem Cell Culture • 3D Cell Cultur...

Cell Culture Protocol 9: Testing Cells for Mycoplasma Contamination by Culture Isolation

Related Links • Cell Culture Homepage • Fundamental Techniques in Cell Culture- ECACC Handbook • ECACC Cell Lines • Fetal Bovine Serum • Classic Media and Salts • Cell Culture Reagents • Cell Culture Supplements • Stericup® Media Filtration • Primary Cell Culture • Stem Cell Culture • 3D Cell Cultur...

Cell Chamber Slides & Glass Coverslips - Cell Culture Supplies

Chamber slides are used for viral and mycoplasma testing, chromosome studies, toxicity tests and immunocytology

Common Cell Culture Problems: Contamination

Overview Types of Contamination Common Causes and Prevention of Contaminants Microbial (bacteria, fungi, yeast) Mycoplasma Viral Contamination Chemical Contamination General Tips and Techniques Working within the Biological Safety Cabinet Pipetting and Prevention of Aerosols Disinfection Although ‘c...

Synonyms
Species Mouse (112353)

BEST™ Microbiology Training

Related Resources • Best™ QC Microbiology Training Flyer • Bioburden Testing • Compendial Mycoplasma Testing • Industrial Microbiology Webinars • Sterility Testing • Best™ Services Video • Milliflex Oasis® Services Video • Pharma Services Brochure (PDF) BEST™ programs focus on developing an in depth...

Cell Culture Collections: Exploring the Iceberg

Biowire Spring 2011 — Cell Lines — Models of Disease Cell line misidentification and Mycoplasma contamination are well known as the greatest risks to any practitioner of cell culture1,2. Biological resource centers such as the European Collection of Cell Cultures (ECACC®) exist to ensure that life s...

Common Cell Culture Problems: Cell Death

Related Links • Troubleshooting Guide Home • Poor Cell Growth • Precipitates • Poor Cell Attachment • Cell Clumping • Contamination • Cell Line Misidentification Related Products • Culture media • Fetal Bovine Serum (FBS) • Mycoplasma Detection • Culture Flasks • Trypsin • Antibiotics • Hoechst Stai...

Recommended media for cell lines

We have formed a working partnership with The European Collection of Cell Cultures (ECACC), a world leader and recognized expert in the maintenance, cultivation and distribution of authenticated, validated and mycoplasma free cell lines. Together, we offer the most diverse selection of cell culture ...

ECACC Cell Lines - Cell Culture

We are partnered with The European Collection of Authenticated Cell Cultures (ECACC), a part of Public Health England, to offer their diverse selection of authenticated, validated and mycoplasma free cell lines. Cell lines are for research use only. Please forward request for commercial use to cul...

Cell Culture Fundamentals: Sourcing of Cell Lines

Many cell lines look identical when viewed under a microscope. Cell lines with very different origins and biological characteristics typically cannot be separated on grounds of morphology or culture characteristics. Infection or contamination of a cell line with an adventitious virus or mycoplasma m...

Mammalian Cell Lines

We are partnered with the European Collection of Authenticated Cell Cultures (ECACC) repository, part of Public Health England, to offer quality-controlled cell lines and nucleic acids to laboratories around the world. Browse our portfolio of authenticated, validated, mycoplasma-free cell lines for ...

Common Cell Culture Problems: Precipitates

Related Links • Troubleshooting Guide Home • Contamination • Poor Cell Growth • Poor Cell Attachment • Cell Clumping • Cell Death • Cell Line Misidentification • Preventing Cell Culture Contamination (video) • Cell Culture Related Products • Copper(II) chloride dihydrate powder • apo-Transferrin • I...

Cell Culture Antibiotic Selection Guide

• Why use antibiotics in cell culture • Antibiotic kill curve • Antibiotics for Mammalian Cell Culture • Antibiotics for Plant Cell Culture • Antibiotic Selection Agents for Cell Culture Product Name Product No. Gram (+)bacteria Gram (-)bacteria Yeasts Molds Mycoplasma Suggested Working Conc. Amphot...

Biofiles - Life Science Research Product Resource Guide

The Biofiles newsletter provides information regarding products and applications for the biochemicals and reagents we offer for life science research. Issues • Vol. 8, No. 20, Nanodisc Technology: A Revolutionary System for Study of Membrane Proteins • Vol. 8, No. 19, The Role of Inflammation in Neu...

EZview™ Red Affinity Gels [VIDEO]

The EZview™ Red Affinity Gels have a vivid red dye that clearly defines the pellet from the supernatant. This enhanced visibility results in less tedious sample processing and greatly reduces the risk of pellet loss which translates to higher reproducibility and more accurate quantitation of the pro...

Reproducibility Product Spotlight

Validation, rigorous identification, freedom from contamination and batch-to-batch consistency reduce the likelihood of irreproducibility. Supplier management ensures reproducible results over time. ECACC Cell Culture In partnership with The European Collection of Cell Cultures, a world leader and r...

Translational Research Solutions Manufacturing

API & Intermediate Synthesis High Potent Manufacturing Therapeutic Proteins Material & Final Lot Testing Sigma-Aldrich offers an excellent combination of chemistry-enabling technology and established global supply chains which allow for commercial-scale syntheses in cGMP facilities. APIs & Key Inter...

DNA Fragmentation Imaging Kit Troubleshooting

Product No. 06432344001 Low labeling • Fluorescence bleaching may occur upon prologned exposure to bright light. Keep samples in the dark after staining for later inspection. • Permeabilization may not have been effective so that reagents could not reach their target molecules. Ensure that triton-X ...

Cell Culture Protocol 1: Proper Aseptic Technique and Sterile Handling of Cells

Related Links • Cell Culture Homepage • Fundamental Techniques in Cell Culture- ECACC Handbook • ECACC Cell Lines • Fetal Bovine Serum • Classic Media and Salts • Cell Culture Reagents • Cell Culture Supplements • Stericup® Media Filtration • Primary Cell Culture • Stem Cell Culture • 3D Cell Cultur...

Why Use Antibiotics in Cell Culture

Use of antibiotics in cell culture minimizes the loss of valuable cells, reagents, time and efforts due to contamination. Maintenance of aseptic conditions and techniques is vital to a research laboratory that handles cell/tissue culture. Experienced researchers recommend cell culture-tested antibio...

Durapore® PVDF Membrane Filters

The best-known properties of Durapore® Polyvinylidene Fluoride (PVDF) Membranes are their extremely low protein binding and minimal interaction with sample components. Binding less protein per unit surface area compared to nylon, PTFE, or nitrocellulose membranes, Durapore® filters are commonly used...

Cell Culture Reagents -Antibiotics, Buffers and more

The Cell Culture and Insect Cell Culture Tested reagents listed in this section differ from our research grade reagents, in that they undergo additional testing in a cell culture system. This testing is designed to eliminate the need for screening biochemicals prior to use in a cell culture applicat...

Growing Pains: Cell Culture Challenges and Best Practices

Cell culture is an essential technique in modern biological laboratories and is employed in a wide range of research fields, including oncology, genetics, pharmacology, and bioproduction. Cell line contamination and misidentification is a significant threat facing cell culture, with the potential to...

Kapa Biosystems Taq DNA Polymerase and PCR Kits

DNA extraction and amplification are critical steps in your molecular biology workflow and often requires purified, high-quality DNA. Kapa Biosystems offers a robust portfolio of DNA polymerase reagents and PCR kits for extracting and amplifying a variety of mammalian and plant-based genetic materia...

Small Molecule Workflow

When analysing small molecules the overarching need is to maximize sensitivity without compromising reproducibility and laboratory throughput. We have developed unique tools to maximize speed and selectivity throughout the entire workflow. To support the diverse techniques required such as GC, spect...