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Kapa Biosystems PCR Reagents & Kits

Researchers require PCR reagents that reliably perform when they need them. Kapa Biosystems PCR reagents are engineered using a method known as directed evolution for optimal performance in standard PCR, qPCR and qRT-PCR applications. Whether you need Kapa Biosystems PCR products for DNA extraction ...

Complete Solutions for PCR Assay Development

Tools & Resources • PCR Selection Guide • Benefits of nuclease-free water • Custom oligos & probes • PCR MasterMix Calculator • PCR Technologies Guide with Protocols Related Links • CLIA Assay Development • Diagnostic Manufacturing • Elite Products for Diagnostic Manufacturing • Elite vs. cGMP • IVD...

RNAi Question and Answer Archive from the TRC

Experts in the field of RNAi have answered your questions. You will find your answers here. I would like to know whether anyone has used shRNA on primary human monocytes and if so is there any advice? I want to lyse plant cells at various stages and analyze the miRNA (siRNA) population. What vector ...

DNA methylation detection products for your Epigenetics Research

As part of the effort to provide a fully integrated Epigenetic workflow, we provide reagents and kits for DNA Methylation detection and analysis. Methylation-Specific PCR may be performed using our line of JumpStart polymerases; we also provide reagents for qPCR analysis of modified samples. Methyla...

Extract-N-Amp™ Plant PCR Kits Protocol

Catalog Numbers: XNAP2, XNAP2E, and XNAR Product Description Reagents and Equipment Required But Not Provided Precautions and Disclaimer Storage Procedure Troubleshooting Guide Materials References The Extract-N-Amp Plant PCR Kits contain all of the reagents required to rapidly extract and amplify g...

KAPA SYBR® FAST One-Step qRT-PCR Kits FAQs

What are the recommended applications for KAPA SYBR® FAST One-Step qRT-PCR Kits? • Gene expression analysis • Microarray validation • Gene knockdown validation • RNAi and miRNA research What is the difference between a one-step and a two-step qRT-PCR reaction? In a one-step qRT-PCR reaction, reverse...

Oligo Days!

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MISSION® Target-ID Library

MISSION® Target ID Library The Ultimate in Functional miRNA Target Identification The MISSION Target ID Library enables bench-top transcriptome-wide human miRNA and ncRNA gene target identification. With an innovative dual-positive selection system, it makes rapid whole transcriptome miRNA and ncRNA...

Extract-N-Amp™ Plant for ASPB Members

Description The Extract-N-Amp™ Plant PCR Kits contain all the reagents necessary to rapidly extract genomic DNA from plant leaves and amplify targets of interest by PCR. • Novel – single-step extraction of genomic DNA to PCR • Fast – tissue to PCR in 15 minutes • Convenient – no long enzymatic diges...

Extract-N-Amp Seed PCR Protocol

Catalog Number: XNAS2 Storage Temperature -20 °C Product Description Reagents provided Reagents and equipment required, not provided Precautions and Disclaimer Storage Procedures Related Products Troubleshooting Guide Materials References The Extract-N-Amp Seed PCR Kit contains all the reagents need...

Regulatory Agencies & Conclusions - PCR Technologies Guide

PCR Technologies Guide Historical Timeline Polymerase Chain Reaction Quantitative PCR Digital PCR Quantitative PCR and Digital PCR Detection Methods PCR/qPCR/dPCR Assay Design Sample Purification and Quality Assessment Reverse Transcription Assay Optimization and Validation Data Analysis Troubleshoo...

Imprint® DNA Modification Kit Protocol

• Product Description • Kit Components • Storage/Stability • Workflow • Product Profile • Procedure • Troubleshooting • Frequently Asked Questions • Materials • Materials Required Not Provided • References The Imprint® DNA Modification Kit provides the reagents needed for bisulfite conversion and po...

KAPA SYBR® FAST qPCR Kits FAQs

What are the recommended applications for KAPA SYBR® FAST qPCR Master Mix (2X) Kits? Gene expression analysis • Microarray validation • Low-copy gene detection • Gene knockdown validation • ChIP • Next-generation sequencing library quantification What is the enzyme in the KAPA SYBR® FAST qPCR Master...

Extract-N-Amp Blood Kit

High-throughput genetic analysis of blood requires a rapid, automated solution for genomic DNA extraction. Standard methods for purifying DNA from blood can be laborious, not amenable to automation, and unnecessary for robust downstream techniques such as PCR. The Extract-N-Amp Blood PCR Kit is a hi...

Custom & Predesigned esiRNA

All ordering and quotation options are in the esiRNA Specifications table. MISSION esiRNA—powered by Eupheria Biotech--provide RNAi researchers with a proven, cost-effective, and simple way to perform post-transcriptional silencing of protein-coding genes and lncRNA (long non-coding RNA). Biological...

Cell Culture Collections: Exploring the Iceberg

• • Download PDF • Biowire Spring 2011 — Cell Lines — Models of Disease Cell line misidentification and Mycoplasma contamination are well known as the greatest risks to any practitioner of cell culture1,2. Biological resource centers such as the European Collection of Cell Cultures (ECACC®) exist to...

TransPlex

View Other Articles | Download a PDF of this article TransPlex® RNA Amplification Kit TransPlex Complete Whole Transcriptome Amplification Kit: Whole Transcriptome Amplification of Highly-Degraded RNA from FFPE Tissues Ken Heuermann and Brian Ward Research and Development, Biotechnology Division, Si...

Sequencing - Buffers, Markers and Reagents for Sequencing

We provide high-quality products for use in both Sanger and Next Generation Sequencing workflows. • Sample stabilization • DNA isolation kits ○ Whole Genome Amplification Kits – Seqlex DNA Amplification Kits – UltraClear™ Clean-up Kit • Custom NGS Oligos • Custom DNA Oligos ○ GenElute™ PCR Clean-up ...

Extract-N-Amp™ Tissue PCR Kit Protocol

Catalog Numbers: XNAT2, XNAT2R Product Description Reagents and Equipment Required But Not Provided Precautions and Disclaimer Storage Procedure PCR amplification Troubleshooting Guide Materials References The Extract-N-Amp Tissue PCR Kit contains all the reagents needed to rapidly extract and ampli...

New Lab Start-Up Product Savings

Sigma-Aldrich New Lab Start-Up Program – Product Savings With the Sigma-Aldrich New Lab Start Up Program, you will receive: Savings on Essential Life Science Products Cell Culture Discounts (up to) Liquid Media* 50%* Powdered Media 20% Antibiotics 15% Growth Factors 10% Reagents 20% PCR Reagents Dis...

TransPlex® Whole Transcriptome Amplification Protocol

• Product Description • Components • Storage • Procedure • Troubleshooting • Frequently Asked Questions • Labeling Protocols for Microarrays • Posters and Publications • Materials • References TransPlex, a Whole Transcriptome Amplification (WTA) method, allows for representative amplification of nan...

Epigenetic Mechanisms in Cancer Pathogenesis

Cancer accounted for approximately 13% of all deaths in 2004, according to the cancer fact sheet published by the World Health Organization (WHO) in February 2009. The term cancer (malignancy) describes a diverse class of diseases characterized by uncontrolled cell growth, which frequently culminate...

Spectrum™ Plant Total RNA Kit for Difficult Plant Tissues

The total RNA purification method for difficult plant tissues Few methods are capable of delivering desired amounts of total RNA from plant tissues that contain high levels of secondary metabolites. The Spectrum™ Plant Total RNA Kit was designed to be different. Incorporating a new, patent-pending l...

Essential Techniques and Protocols for Cell Culture

Whether you’re growing your first cells or if you’re just looking for a refresher course, you’ve come to the right place. Our protocols will help you Prepare, Grow and Analyze your cells with confidence. See our Cell Culture Video Tutorials for additional learning opportunities. • Safety aspects of ...

KAPA2G Fast Multiplex Kits

For which applications should I use KAPA2G Fast Multiplex PCR Kit? The KAPA2G Fast Multiplex PCR Kit is ideally suited for end-point, fast multiplex PCR of multiple DNA fragments, ranging in size from 50–1500 bp. This kit is ideally suited for: • Typing of transgenic organisms • Amplification of mic...

GenElute™ HP Plasmid Maxiprep Procedure [VIDEO]

The GenElute™ HP Plasmid Maxiprep kit offers a simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cutures. The recovered plasmid DNA is ready for immediate use in downstream applications, such as restriction digestion, ligation, sequencing, PCR, transformatio...

Extract-N-Amp Plant Kit: High-Throughput System for the Rapid Extraction of Genomic DNA

Large-scale plant genomic and breeding studies have created the need for automated methodologies for genomic DNA preparation. The Extract-N-Amp™ Plant PCR kit is a high-throughput system for the rapid extraction and subsequent amplification of genomic DNA from plant leaves (Figures 1, 2, 3 and 4). S...

Inducible shRNA Vectors – tight regulation meets great gene silencing

tight regulation meets great gene silencing Request a Quote Background Ordering and Service Information Technical Bulletin Performance IPTG Inducible shRNA References Background Dial into the latest development from the RNAi Consortium (TRC) Constitutively expressed shRNAs are useful for most RNAi n...

Options for Sample Collection, Transport, Archiving, and DNA Purification

Extracted from Nucleic Acid Sample Preparation for Downstream Analyses, GE Healthcare, 2007 After sample collection, the other steps do not follow in a specific order. For example, DNA can be prepared from fresh sample, or the sample can first be transported off-site then stored (short term) or arch...

Whole Genome Amplification for Single Cell Biology - Technical Article

The ultimate biological unit lies within a single cell. Many biological disciplines have taken aim to elucidate the causes of cellular differentiation at this level. The secret triggers that signal human maturation, regeneration and genetic diseases all lie buried in a single cell that was originall...