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transformation(18)
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363 matches found for precipitation

Research Essentials - Technical Library

Buffer pKa/pH Tables and Formulas: Useful pH Ranges of Selected Biological Buffers (25 °C, 0.1M) Trizma Buffer Table - pH vs. Temperature Phosphate Buffer Table - 0.2M solution Commonly Used Electrophoresis Buffers Helpful Formulas DNA / Protein Electrophoresis and Troubleshooting Tables: Effective ...

Boc Resin Cleavage and Deprotection

The most popular reagent for cleavage of peptides from Boc-based resins is anhydrous HF. Of all the cleavage procedures HF appears to be the most versatile and least harmful to a wide variety of peptides synthesized on Boc-based resins. The major drawback of this procedure remains its highly toxic a...

DNA / Protein Electrophoresis and Troubleshooting Tables

• Effective Range of Separation of DNA in Polyacrylamide Gels • Effective Range of Separation of DNA in Agarose Gels • DNA Size Migration of Sample Loading Dyes • Troubleshooting Guide for SDS-PAGE Protein Electrophoresis % Acrylamide (w/v)1 Efficient Range of Separation (bp) 3.5 1000-2000 5.0 80-50...

Phycology Media - Plant Tissue Culture Protocol

• Preparation from Packaged Powder • Media Composition Table Phycology media can be prepared from concentrated solutions or from powdered salt mixtures. The concentrated solutions are complete, including vitamins, and should be stored frozen. The powdered media allow flexibiltiy in customizing the f...

Protocol for Conjugating NHS-Ester Modifications to Amino-Labeled Oligonucleotides

This protocol is for conjugating NHS-ester modifications, such as TxRd (Sulforhodamine 101-X) (protocol is effective for most NHS-ester modifications), to an oligonucleotide with an amino label, such as 5'-Amino-Modifier C6 (Figure 1). Since TxRd (Sulforhodamine 101-X) and several other modification...

Chemical Synthesis of Monodisperse Magnetic Nanoparticles

• • Download PDF • Sheng Peng and Shouheng Sun* Department of Chemistry Brown University Providence, Rhode Island 02912, USA *E-mail: ssun@brown.edu Magnetic nanoparticles have attracted tremendous attention due to their novel properties and their potential applications in magnetic recording, magnet...

Performing High-Throughput Screening Using His MultiTrap HP and His MultiTrap FF 96-Well filter Plates

Related Articles • Affinity Chromatography in a Purification Strategy (CIPP) • Characteristics of Dextrin Sepharose® High Performance Products • Characteristics of Glutathione Sepharose ® Products • Characteristics of Ni Sepharose®, Ni Sepharose® excel, TALON® Superflow™, and Uncharged IMAC Sepharose®...

GenElute 96 Well Blood Genomic DNA Purification Kit Protocol

The starting materials are lysed at room temperature in a lysis buffer which contains chaotropic salts in the presence of Proteinase K. The addition of ethanol ensures the appropriate conditions to bind the DNA to the silica membrane of the Blood Binding Plate. After washing the bound DNA to remove ...

ProteoPrep Depletion Products

Product Name Product # Function Samples Processed Input Sample Size Output Compatibility ProteoPrep® Immunoaffinity Albumin & IgG Depletion Kit PROT-IA Depletes HSA and IgG from serum or plasma using small recombinant immunoaffinity ligands 10 10µL - 50µL Two-dimensional electrophoresis (2DE) Proteo...

Few Monolayer Atomic Layer Deposition (ALD) on Surfaces and Interfaces for Energy Applications

Department of Mechanical Engineering and Materials Science, One Brookings Drive, Washington University in St. Louis, St. Louis, MO 63130 *Email: parag.banerjee@wustl.edu Atomic layer deposition (ALD) exploits a sequential set of two or more surface reactions repeated cyclically to achieve thin film ...

Ion-Suppression & Phospholipid Contamination | HybridSPE

Related Articles • Investigating Matrix Interference in Analysis of Antiarrhythmic Cardiac Drugs in Plasma • HybridSPE-Phospholipid Technology • Comparison of Sample Preparation Techniques • Improve LC-MS Run Time/Performance & Reduce Risk of Ion-Suppression • HybridSPE-PL Method Development: Recove...

Purification from unclarified cell lysate using HisTrap FF crude

Related Articles • Affinity Chromatography in a Purification Strategy (CIPP) • Characteristics of Dextrin Sepharose® High Performance Products • Characteristics of Glutathione Sepharose ® Products • Characteristics of Ni Sepharose®, Ni Sepharose® excel, TALON® Superflow™, and Uncharged IMAC Sepharose®...

Two Distinct Sample Prep Approaches to Overcome Matrix Effect in LC/MS of Serum or Plasma Samples

Related Articles • Reporter US Volume 33.1 (4 Mb PDF) • Bioanalytical Microsampling using Biocompatible SPME and Benefits over Dried Blood Spots • Investigating Matrix Interference in Analysis of Antiarrhythmic Cardiac Drugs in Plasma • Method Optimization for LC-MS Analysis of Vitamin D Metabolite ...

Dextran Sulfate

Cas #: 9011-18-1 Dextran sulfates are supplied as the sodium salt forms, making them soluble and stable in water. Dextran sulfate contains approximately 17% sulfur which is equivalent to approximately 2.3 sulfate groups per glucosyl residue. Dextran is a polymer of anhydroglucose. It is composed of ...

From Form to Function: Molding Porous Materials in Three Dimensions by Colloidal Crystal Templating

• • Download PDF • Melissa A. Fierke, Fan Li, and Prof. Andreas Stein Department of Chemistry, University of Minnesota Methods of structuring materials with submicrometer features in two dimensions have become highly sophisticated. For example, lithographic methods are now capable of carving out sev...

Extract-N-Amp™ Plant Product Information

Description Procedure Application Features and Benefits Order Information Kit Contents Sample Data The Extract-N-Amp Plant PCR Kits contain all the reagents necessary to rapidly extract genomic DNA from plant leaves and amplify targets of interest by PCR. • Novel – single-step extraction of genomic ...

Isopropanol Molecular Biology Reagent

CAS RN 67-63-0 EC 200-661-7 Synonyms: 2-Propanol, sec-Propyl alcohol, IPA, Isopropyl alcohol Isopropanol is a polar organic solvent that is widely used in chemistry, biochemistry and molecular biology. Industrial applications include its use in antifreezes, quick-drying oils, and quick-drying inks, ...

TriPure Isolation Reagent Protocol

Product No. TRIPURE-RO Isolation of nucleic acids and proteins from plant sample material, e.g. leafs Roche has not tested this experimental protocol that has reportedly worked successfully for at least Dandelion and Philodendron samples. In general, yields are better for plants having thin leaf wal...

Extracellular Vesicle Preparation

Extracellular vesicles (EVs), such as exosomes, are fascinating particles that can deliver proteins, lipids, and RNAs to distant target cells. Critical to understanding EV function is reliable enrichment of pure fractions. Many investigators use ultracentrifugation, which is lengthy and labor intens...

Depletion of Phospholipid Matrix Interference when Dealing with Small Volume Plasma Samples

• Sample preparation of small volume plasma samples can be restrictive due to the limited volume of available plasma. • Mouse studies, plasma samples are limited to 30 μL or less. • Traditional solid phase extraction techniques limited due to large hold up volumes. • Often limited to liquid-liquid p...

n-Butanol Molecular Biology Reagent

CAS RN 71-36-3 EC 200-751-6 Synonyms: 1-Butanol, Butyl Alcohol n-Butanol is useful for the removal of ethidium bromide from DNA purified by CsCl gradient ultracentrifugation (Sambrook, p. 1.46). It is also suitable for use in the concentration of dilute nucleic acid solutions by repeated extractions...

Affinity Chromatography

Affinity chromatography is the preferred method of bioselective adsorption and subsequent recovery of a compound from an immobilized ligand. Each is designed for highly specific and efficient purification of proteins and related compounds. We offer appropriately selective ligands on beaded and porou...

Recovery Optimization | HybridSPE-PL Method Development

The HybridSPE-PL stationary phase is comprised of a proprietary patent pending zirconia bonded silica particle. Phospholipids are retained/removed from the sample through a highly selectivity Lewis acid-base interaction between the phosphate group (Lewis base), inherent of all phospholipids, and Zr ...

Chiral and Achiral LC-MS Analysis of Warfarin™ in Plasma

Related Articles • Reporter US Volume 31.2 (3 Mb PDF) • Rapid, Sensitive, and Quantitative LC/MS/MS Determination of Digitoxin and Digoxin in Plasma • Accurate Biological Testing for Amphetamine and Methamphetamine Abuse Using Chiral HPLC and MS Detection • Fast and Robust HPLC Separation of Bile Ac...

Free Radical Initiators

Photoinitiators | Thermal Initiators | Stabilizers Initiator/Stabilizer FAQ Dr. S.S. Newaz Polyorganix Inc., Houston, TX Q. How does one choose the appropriate initiator? In a free radical addition polymerization, the choice of polymerization initiator depends mainly on two factors: a) its solubilit...

Dextran

Cas #: 9004-54-0 Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(1-6) linkages. The remaining a(1-3) linkages account for the branching of dextran.1,2,3 Conflicting data on the branch lengths implies that the average branch length is less than three glucose units...

Selecting Correctly Expressing Recombinants

• Blue White Screening • DNA Minipreps • Screening by Restriction Digestion • Screening by PCR • Confirm cut plasmid sizes by agarose gel electrophoresis • DNA Maxipreps • DNA Precipitation • RNase Treatment • Clean-up of DNA Blue / White colony screening is a strategy to quickly and easily distingu...

Michael Krische Group – Products available at Aldrich from the Krische Laboratory

Professor Product Portal Index Professor Michael Krische The Krische group has developed a broad class of “C-C bond forming transfer hydrogenations” wherein the exchange of hydrogen between alcohols and unsaturated reactants serves to generate aldehyde-organometal pairs that combine to give products...

Fullerene-Based n-Type Semiconductors in Organic Electronics

• • Download PDF • †Solenne BV, The Netherlands ‡Molecular Electronics, Zernike Institute for Advanced Materials, and Stratingh Institute of Chemistry, University of Groningen, The Netherlands Since the first publication in 1995 describing a bulk heterojunction photodiode incorporating a methanofull...

Vitamin Mixtures - Plant Tissue Culture Protocol

• Preparation of Solutions from Packaged Powder • Media Preparation using Vitamin Solutions • Vitamin Mixtures Composition Table Powdered vitamin mixtures are hygroscopic and must be protected from atmospheric moisture. The entire contents of each package should be used immediately after opening. Th...