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220 matches found for proteomics

Cell Biology | Highest Quality and Largest Selection of Biomolecules

Enhance your understanding of biology with our world-class biomolecules. Our portfolio includes over 50,000 primary antibodies, 12,000 of which are Prestige Antibodies® powered by Atlas Antibodies, as well as a vast range of proteins, bioactive peptides, and kinases. Offering over 3,000 Bioactive Sm...

Stem Cell Biology — Where We’ve Been and Where We’re Heading

BioFiles 2008, 3.11, 4. Dr. Greg Wall, Ph.D. Unraveling and exploring the mysteries of life have been driving forces behind scientific studies since Aristotle first attempted to understand the origins of life. Through the eyes of the microscope, cellular processes have been observed, investigated, u...

MALDI Mass Spectrometry | BioFiles Volume 5 Number 2 Protein Characterization & Detection

BioFiles Volume 5, Number 2 — Protein Characterization & Detection Download Biofiles v5 n2 (3.4 Mb PDF) Table of Contents • Introduction • Labeling of Proteins • X-ray Crystallography/Crystallization • MALDI Mass Spectrometry • Colorimetric Electrophoresis Stains • IEF Focusing • Western Blotting • ...

Mesoporous Materials

Adam Raw Product Line Manager Materials with specially ordered porous features on the nano-scale have important applications in optics, catalysis, drug delivery systems, coatings, cosmetics, bio-separation, diagnostics, gas-separation and nanotechnology. Nanoporous materials consist of an amorphous ...

Human Protein Atlas Webinar

A Human Protein Atlas Mathias Uhlen, AlbaNOva University Center, Royal Institute of Technology, Stockholm, Sweden The completion of the human genome sequence has opened up a possibility for global expression profiling of human tissues and cells, allowing for comparative studies between normal and di...

Recombinant Protein Purification and Detection

The expression, purification, and detection of recombinant proteins can be a lengthy and time-consuming endeavor. Depending on the protein of interest and expression system, protein tags may be added to improve the outcome of common steps, including protein solubility during expression, purification...

Poly(Amino Acid) Block Copolymers for Drug Delivery and other Biomedical Applications

Carmen Scholz Department of Chemistry, University of Alabama in Huntsville Huntsville, AL 35899 Email: scholzc@uah.edu Humankind has utilized protein materials throughout its existence, starting with the use of materials such as wool and silk for warmth and protection from the elements and continuin...

Prestige Antibodies® Powered by Atlas Antibodies in Western Blot Application

Western Blot Western Blot (WB) is considered to be the most sensitive, simple and unequivocal immunodetective application available. This is due to the high amount of information obtained upon performance, such as target molecular weight and quantitative, as well as qualitative, information of the p...

Prestige Antibodies® in Immunohistochemistry

IHC antibodies and the Human Protein Atlas Immunohistochemistry (IHC) is the most widely used technique in histopathological diagnosis and research for detection of proteins in tissues and cells. Today, IHC can be applied in a high-throughput fashion for studying proteins using Tissue Microarrays (T...

Product Directory Home

Analytical / Chromatography • Analytical Standards • Applications • Derivatization Reagents • Gas Chromatography • HPLC • LPLC/TLC/Paper Chromatography • Microbiology • Resins & Media • Sample Prep/Purification • Solid Phase Microextraction • Syringes • Titration • Vials • More.. Materials Science •...

Proteomics - Learning Center

• Application Notes • Automation Protocols • Cold Spring Harbor Protocols • Protein Electrophoresis Troubleshooting Tables • Proteomics Literature • Proteomics Poster Gallery • Recombinant Protein Expression Workflow

Organelle Isolation | Sample Preparation

A fundamental of cell biology is the biogenesis and maintenance of intracellular organelles. Eukaroytic cells are organized by segregating essential cellular functions into discrete compartments composed of proteins and lipids. Establishing and maintaining order requires mechanisms to synthesize and...

Western Blot Resources

Protect Your Proteins Eliminate vulnerabilities in your western workflow Western blotting, also known as immunoblotting, is a well established and widely used technique for the detection and analysis of proteins. This detection method is based on building an antibody/protein complex via specific bin...

Proteomics - Cell Lysis & Protein Extraction

Cell lysis and high yield protein extraction are important to quality recombinant protein purification. CelLytic products include protein extraction formulations for bacterial cell lysis, mammalian cell lysis, yeast cell lysis and plant cell lysis. We also carry protease inhibitor cocktails to maint...

Unique Features of Prestige Antibodies®

Prestige Antibodies Prestige Antibodies are highly characterized antibodies with all characterization data for each target protein accessible through the Human Protein Atlas portal (proteinatlas.org). The uniqueness and low cross-reactivity of the Prestige Antibodies to other proteins are due to a t...

Mapping the Human Proteome Using Prestige Antibodies

Prestige Antibodies and the Human Protein Atlas For the first time ever, a comprehensive localization analysis has been performed on 10,000+ human proteins in a standardized set-up manner1,2. The analysis has been performed in several levels using different methodologies. All results are presented o...

HIS-Select Supporting Products Reagents

• Products for UpStream Processes • Products for DownStream Processes • Reagents and Products for use with HIS-Select™ Products for UpStream Processes Product Name Product No. Cell Lysis Products CelLytic™ B Plus Kit, Lyse 50 grams of fresh or frozen cell paste CB0500 CelLytic™ B Plus Kit, Lyse up t...

Proteomics BIOshell™

Solutions for Proteomics Digestion/Labeling: BIOshell™ Fused-Core™ columns Discover the latest Proteomics solutions and receive 25% discount on BIOshell™ Fused-Core™ columns. Reference promo code HLH when placing your order. Offer valid from 05.03.19 - 30.06.19 Product No. Description % to Cart 5099...

A Quantitative Mass Spectrometry-Based Approach for Identifying Protein Kinase Clients and Quantifying Kinase Activity

Huang et al.1 have recently developed a quantitative mass spectrometry-based assay for identifying protein kinase substrates and quantifying kinase activity. This approach offers several advantages over current methods: • It is label-free and eliminates the need for fluorescent or radiolabel detecti...

MS Qual/Quant QC Mix

Still Using BSA to Standardize Your Mass Spec QC Runs? Switch to Sigma’s New MS Qual/Quant QC Mix Experience the newest product available in proteomic analysis. MS Qual/Quant QC Mix (Cat. No. MSQC1) allows you to benchmark and monitor the daily performance of both qualitative and quantitative proteo...

Fluorous Literature Highlights for Small Molecule Synthesis

Review Articles Fluorous Separation Media Fluorous Protecting Groups and Tags Fluorous Scavengers and Reagents Fluorous Catalysts and Ligands Fluorous Mixture Synthesis (FMS) Microwave-Assisted Fluorous Synthesis Fluorous Proteomics and Metabolomics Review Articles 1. Zhang, W.; Curran, D. P. Synthe...

Proteomics Literature

Epitope Tags in Protein Research Mini Guide HIS-Select™ Quick Reference Guide (PDF) Glycbiology Analysis Manual Tools for Mass Spectrometry (PDF) Integrated Solutions for MALDI–MS (PDF) Proteomics and Protein Expression Product Guide

Transplex® RNA Amplification Kit: Whole Transcriptome Amplification of Highly-Degraded RNA from FFPE Tissues.

Ken Heuermann and Brian Ward Research and Development, Biotechnology Division, Sigma-Aldrich The TransPlex Complete Whole Transcriptome Amplification (WTA2 ) Kit effectively amplifies intact and highly degraded RNA. To benchmark maintenance of representative RNA levels during amplification, differen...

Introduction to Synthetic Biology

Synthetic Biology is the combination of engineering principles and molecular biology techniques to produce novel biological solutions. We offer building blocks for synthetic biology projects, including oligonucleotides and CRISPR genome editing reagents, as well as custom engineered cell lines and m...

Vol 3 No 2

ChemFiles Vol. 3, No. 2 Products For Biomolecular NMR Download PDF version (1.49 MB) Page 1: Intro / Labeled Products for Cell Free Synthesis / Products for Peptide Synthesis / Products for Minimal Media Page 2: Labeled Complex Growth Media / Buffers and Reagents / a-Keto Acids / Labeled Nucleotides...

A New Cancer Biomarker Discovered

Need for Biomarkers in Cancer Prognosis Cancer is a common disease and a major cause of death in the Western world. In general, incidence rates increase with age for most cancer forms. As human populations continue to live longer due to an improvement of the general health status, cancer will affect...

Read the latest developments in CRISPR research

read abstract Unconventional Trafficking of Mammalian Phospholipase D3 to Lysosomes Adriana CarolinaGonzalez1MichaelaSchweizer2SebastianJagdmann1ChristianBernreuther3ThomasReinheckel4PaulSaftig1MarkusDamme15 23-01-2018 Variants in the phospholipase D3 (PLD3) gene have genetically been linked to late...

How Transfection Works [VIDEO]

Transfection is the process that introduces DNA or RNA into eukaryotic cells. It is a common and essential method to study and modulate gene regulation, which allows for a better understanding of gene function and protein expression within the context of the cell. Transfection can be used for analys...

The Protein AQUA Strategy - enabling absolute protein quantitation

In June 2003, Dr. Steve Gygi and his team presented an innovative strategy, Protein AQUA, enabling absolute protein quantitation using stable isotope labeled peptides and HPLC-MS. By applying a common principle, the use of a labeled molecule as an internal standard, to protein analysis, Gygi’s team ...

Aldrich Fluorous Chemistry

FluoroFlash® Technology for Synthesis and Separation — Fluorous chemistry has proved to be very efficient as a general, robust, selective, and orthogonal separation method to isolate a desired product from crude reaction mixtures. In partnership with Fluorous Technologies, Inc., we are pleased to of...