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1055 matches found for purification

FLAG® System Literature

The FLAG Expression System is the proven and trusted method to express, purify and detect recombinant fusion proteins. Listed below are many references linked to PubMed, where you can find complete abstract information and relevant journal information. Tell us! We’re interested in knowing how our FL...

FLAG tag: Epitope tag for recombinant protein purification, detection and expression

With proven utility in numerous applications, FLAG® tags provide superior detection and purification of recombinant fusion proteins. The FLAG® epitope tag is a short, hydrophilic, 8-amino acid peptide (DYKDDDDK tag), that is readily cleavable by enterokinase (Ek). Due to its small size and hydrophil...

ANTI-FLAG® M2 Magnetic Beads | Recombinant Protein Purification & Detection

The FLAG Expression System is a proven method to express, purify and detect recombinant fusion proteins. We now offer a magnetic bead for immunoprecipitation, protein purification, and the study of protein-protein interactions. The ANTI-FLAG M2 Magnetic Bead is composed of murine derived, anti-FLAG ...

FLAG® HA System

Isolation and analysis of protein complexes continues to be a major technical challenge in function proteomics research. Co-immunoprecipitation is the most widely used method for isolation of protein complexes. The TAP (Tandom Affinity Purification) methodology incorporates tandem linked affinity ta...

Roche Products and Biochemical Reagents

Whether working within a competitive funding landscape or tasked with hitting key business milestones towards financial deliverables, research scientists are also presented with more scientific information and challenges than ever before. As a result, researchers require reliable reagents that are a...

Nutrition Research | Dietary supplements, vitamins, botanicals, herbal extracts, nutrients

Nutritional Research investigates the relationships between diet and health, disease, and metabolism by studying nutrient analysis, chemoprevention, bioavailability, and nutrient interactions. We offer a wide range of biochemicals commonly found in plants, food/beverage and metabolism. Also, our por...

HIS Select®: A Highly Selective Chemistry for HIS-tagged Protein Purification

Our HIS-Select products have the ability to purify histidine-tagged proteins quickly and with high selectivity. This is due to HIS-Select's patented, non-charged, hydrophilic nickel chelate linkage. This non-charged linkage greatly diminishes non-specific binding. Most other systems for histidine-ta...

Roche Distribution Agreement Q&A

Roche Biochemical Reagents On July 1, 2015 we signed an exclusive, global distribution agreement for Roche Biochemical Reagents. This agreement was then renewed for another five years in 2019. Products covered by the agreement include: • Genomics - Products for DNA labeling and detection, restrictio...

Small Molecules Drug Development

When analyzing small molecules the overarching need is to maximize sensitivity without compromising reproducibility and laboratory throughput. We have developed unique tools to maximize speed and selectivity throughout the entire workflow. Our tools support the requisite techniques, such as gas chro...

MaxGel™ ECM Hydrogel: An In Vitro Human Cell Derived Basement Membrane Extract for 3D Cell Culture Applications

MaxGel™ ECM is an in vitro derived human basement membrane extracts (BME) that provides a rich three-dimensional environment to promote cellular proliferation for multiple cell types. MaxGel™ ECM contains extracellular matrix components including collagens, laminin, fibronectin, tenascin, elastin, a...

HIS-Select Convenient Formats

• HIS-Select® iLAP® 5 ml Column • HIS-Select® High Flow Cartridges • HIS-Select® Cartridge • HIS-Select® Spin Column • EZView™ Red HIS-Select® Nickel Affinity Gel HIS-Select® iLAP® 5 ml Column, (H9913) The HIS-Select iLAP (Integrated Lysis and Affinity Purification) 5 ml Column is a single-use, disp...

KAPA Long Range PCR Kits FAQs

For which applications should I use KAPA Long Range PCR Kits? • PCR amplification of long targets and/or PCR using low amounts of template DNA. • Standard short- and mid-range PCR amplification. • Amplification of PCR products to be used for ligation into 3′-T-overhang cloning vectors. What is the e...

Amplification of Genomic DNA using REDTaq DNA Polymerase

Background Reagents Procedure References Materials REDTaq DNA Polymerase is a convenient package that includes all the necessary components for a PCR reaction except primers, DNA template and water. The formulation has been optimized for amplification of genomic or other complex DNA templates. REDTa...

Analytical Chemistry & Chromatography Products

• Air Monitoring • Analytical Reagents • Analytical Standards • Applications • Chiral Chromatography • Chromatography Standards • Derivatization Reagents • Food & Beverage Analysis • Gas Chromatography • HPLC • Ion Sensor Materials • Karl Fischer Titration • Laboratory Supplies • LPLC/TLC/Paper Chro...

Small Molecule Workflow

When analysing small molecules the overarching need is to maximize sensitivity without compromising reproducibility and laboratory throughput. We have developed unique tools to maximize speed and selectivity throughout the entire workflow. To support the diverse techniques required such as GC, spect...

HIS-Select® iLAP®

Screening a large number of recombinant clones for the production of soluble target proteins can be time consuming and laborious. Sigma’s patented HIS-Select® iLAP® (Integrated Lysis and Affinity Purification) column and plate technology meets the need of researchers who screen large numbers of solu...

mini Quick Spin Oligo Columns Troubleshooting

Product No. 11814397001 Nucleotide contamination of final sample for mini Quick Spin Columns or Quick Spin Columns Possible reasons are: 1. Sample should not be applied to the side of the column, so that molecules to flow around the gel matrix; instead the sample should pass through the matrix (for ...

KAPA Mouse Genotyping Kit FAQs

What are the recommended applications for the KAPA Mouse Genotyping Kit? KAPA Mouse Genotyping Kits are ideally suited for the rapid extraction and amplification of DNA from mouse tail, ear and toe tissue. The kits are also suitable for the extraction and amplification of DNA from other animal tissu...

Additional Products

• Main Page • Purification & Enrichment • Characterization & Detection • Protein Deglycosylation Featured Products • Additional Products for Glycoprotein Analysis • Additional Products for Phosphoprotein Analysis • Trypsin In-Gel Digest Kit • Phosphatase Inhibitor Cocktails • Chart of mass changes r...

DNA & RNA Purification GenElute Endotoxin-free Plasmid Kits

Description: Endotoxins (also known as lipopolysaccharides or LPS) are often co-purified with plasmid DNA and can significantly reduce transfection efficiencies in endotoxin-sensitive cell lines. The GenElute™ Endotoxin-free Plasmid Kits offer a simple, rapid, cost-effective method for purifying pla...

Sigma-Aldrich Product Portfolio

Sigma-Aldrich gives you easy access to the product you need • by streamlining our product range • by delivering clearly defined product categories • by providing brands and grades for every application Sigma-Aldrich Brands PRODUCT BRANDS Supelco® Sigma® Sigma-Aldrich® SAFC APPLICATIONS Analytical Ca...

Sigma Life Science Online Product Catalog

Biochemicals & Reagents • Amino Acids • Antibodies • Antibiotics • Biological Buffers • Carbohydrates • Detergents • Enzymes, Inhibitors, and Substrates • Life Science Labware • Lipids • Peptides • Proteins & Derivatives • Stable Isotopes • Additional Biochemicals & Reagents Techniques & Application...

Column Packing and Preparation for Hydrophobic Interaction and Reversed Phased Chromatography

Prepacked columns from GE Healthcare will ensure reproducible results and the highest performance. Use small prepacked columns for media screening and method optimization to increase efficiency in method development, for example, HiTrap™ HIC Selection Kit, RESOURCE™ HIC Test Kit and RESOURCE™ RPC co...

Removal of Thrombin and Factor Xa Using HiTrap Benzamidine FF (High Sub)

To protect the fusion protein from proteolytic degradation prior to enzymatic cleavage with PreScission Protease, thrombin, or Factor Xa, it may be necessary to remove proteases from the sample. Additionally, following enzymatic cleavage, it may be necessary to remove thrombin or Factor Xa from the ...

Generic Pharmaceutical & Release Testing

Microbial Testing Solutions We offer a broad range of products for microbiological testing to ensure accurate QC and absolute safety of your end product. Your quality requirements are the driving force for providing you with the quality you need! • Bioburden • Sterility Testing • Environmental Monit...

HIS-Select Information

A Highly Selective Chemistry for HIS-tagged Protein Purification Sigma's HIS-Select products have the ability to purify HIS-tagged proteins quickly and with high selectivity. This is made possible by the non-charged linkage chemistry that attaches the chelate to the agarose bead matrix. Most other i...

Isolation of Plant and Fungal RNA from Challenging Samples using Norgen's Plant/Fungi RNA Purification Kit

Norgen’s Plant/Fungi Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA from a wide range of plant and filamentous fungal species. Total RNA can be purified from fresh or frozen plant tissues, plant cells or filamentous fungi samples using this kit. Al...

mini Quick Spin RNA Columns Troubleshooting

Product No. 11814427001 Poor sample recovery of mini Quick Spin Column or Quick Spin Column Possible reasons for poor sample recovery: 1. Centrifugation speed is too fast or centrifugation time is too short: Do not centrifuge the columns faster than recommended speed. 2. Matrix is not evenly resuspe...

Glycosylation

Glycobiology’s contribution to life science is increasingly being recognized. To this end, we offer a wide range of products for research into the functional and structural analysis of glycans, including glycoproteins, proteoglycans, glycolipids and free oligosaccharides. Browse our offering of glyc...

Hybridoma Fusion Partners Cell Lines

Hybridoma technology involves the production of monoclonal antibodies specific to an antigen of interest. The somatic fusion of B lymphocytes of the spleen with immortal myeloma cells gives rise to a hybridoma cell line that can be perpetually propagated to produce clonally identical antibodies, as ...