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103 matches found for recombination

Nucleofection of ZFN Reagents [VIDEO]

This video demonstration will begin with an overview of the nucleofection laboratory exercise and experimental layout before moving onto the washing of cells to remove serum, preparation of ZFN reagents, nucleofection of samples, and summary of the expected exercise results. In this exercise we will...

Biosafety - MISSION shRNA FAQs

How serious are lentiviral biosafety concerns? Is there a realistic risk of HIV infection? Viral vector systems have been developed with enhanced safety features. It is recommended to use a third generation lentiviral vector system. It is a three plasmid system consisting of: 1. The packaging vector...

Zinc Finger Nuclease Efficiency

Zinc Finger Nuclease (ZFN) technology has provided researchers with a tool for integrating exogenous sequences into most cell lines or genomes in a precise manner. Using current methods, the efficiency of targeted integration (TI) into the host genome is generally low and is highly dependent on the ...

Conducting Polymer Materials for Flexible OPV Applications

Related Products • Conductive Polymers • Organic Photovoltaic (OPV) Materials • Organic and Printed Electronics Related Articles • Nanoparticle-based Zinc Oxide Electron Transport Layers for Printed Organic Photodetectors • Inorganic Interface Layer Inks for Organic Electronic Applications • Applica...

Dye-Sensitized and Perovskite Solar Cells: Interface Engineering by Atomic Layer Deposition

Valerio Zardetto,1 Francesco Di Giacomo,2 Thomas M. Brown,2 Aldo Di Carlo,2 Alessandra D’Epifanio,3 Silvia Licoccia,3 Erwin Kessels,1 Mariadriana Creatore1* 1Department of Applied Physics, Eindhoven University of Technology P.O. Box 513, 5600 MB Eindhoven, The Netherlands 2Centre for Hybrid and Orga...

CompoZr® Zinc Finger Nucleases (ZFNs) for Biological Manufacturing

We recognize that one of the most time consuming and costly endeavors biopharmaceutical companies face is creating robust, efficient cell lines. Zinc Finger Nuclease (ZFN) technology allows for easy creation of novel CHO cell lines with precise and heritable gene additions, deletions or modification...

Genome Modification by Inclusion of loxP Transgene Sequences

Product lists for commonly used protocols from Cold Spring Harbor Protocols. Kazuaki Ohtsubo and Jamey D. Marth This protocol was adapted from "Conditional Mutagenesis of the Genome Using Site-Specific DNA Recombination," Chapter 60, in Gene Transfer: Delivery and Expression of DNA and RNA (eds. Fri...

Restriction Enzymes

Click here to return to the Molecular Biology Guide Restriction endonucleases popularly referred to as restriction enzymes, are ubiquitously present in prokaryotes. The function of restriction endonucleases is mainly protection against foreign genetic material especially against bacteriophage DNA. T...

ChIP Tested Antibodies

Chromatin structure plays a fundamental role in regulating processes that take place on the DNA, such as transcription, replication, and recombination, all of which occur on nucleosomal templates. Therefore, mapping the position of histones selectively modified and histone variants or non-histone co...

recombination activating gene 2
Synonyms RAG-2
Species Human (5897) , Species Mouse (19374) , Species Rat (295953) , Species domestic guinea pig (100723894) , Species Domestic Rabbit (100328951) , Species naked mole-rat (101724205) , Species cow (782387) , Species sheep (101107628) , Species Zebrafish (30658) , Species domestic cat (101096513) , Species domestic cat (493757) , Species dog (483419) , Species chicken (423165)
Summary: This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms. [provided by RefSeq, Jul 2008]

Commercial Volumes of Quantum Dots: Controlled Nanoscale Synthesis and Micron-Scale Applications.

• • Download PDF • Nanoco Technologies, Ltd., Manchester, United Kingdom Quantum dots (QDs) are luminescent semiconductor nanoparticles, with diameters in the range of 1 to 20 nm. The unique optical and electronic properties of QDs are being exploited in a number of applications including flat panel...

AAVS1 Safe Harbor Gene Targeting

A pre-engineered cell line with integrated genetic elements that facilitate simple payload exchange for your specific promoter and gene of interest. The landing pad construct is targeted to the AAVS1 safe harbor locus for stability and contains a florescent marker for downstream screening applicatio...

Ruthenium-based dyes for Dye-sensitized Solar Cells

Related Products • Dye Sensitized Solar Cell (DSSC) Materials • Organic Photovoltaic (OPV) Materials Related Articles • OPV Tutorial • Efficient DSSC for Direct Conversion of Sunlight to Electricity • Preparation of Photoactive Layers for OPV Applications • Synthesis of ZnO Aggregates and their Appl...

Roche Transfection Database

X-tremeGENE™ Transfection Reagents from Roche have been used for a wide range of applications and a vast array of cell lines, primary cells and stem cells. Use the table below to find the right product for your experiment. To request a citation for a cell type on this list, please contact our techni...

Creating Transgenic Mice using CRISPR-Cas9 Genome Editing

Related Content > • Genome Editing Products • Mouse Embryo Validated Reagents • Mouse Embryo and ES Cell Media • Mouse ES Cell Culture Protocols and Procedures • Molecular Biology Reagents • PCR Enzymes While several genome editing tools have been developed in recent years, including zinc finger-bas...

Small Molecule CRISPR Enhancers

As the CRISPR-Cas9 system has become an increasingly popular tool for genome editing, the desire to improve efficiency of precise CRISPR-mediated gene editing has arisen. A number of studies have identified bioactive small molecules that enhance the overall efficiency and targeting precision of CRIS...

Recent Advances in Hybrid Halide Perovskites-based Solar Cells

Kuppuswamy Kalyanasundaram, Shaik. M. Zakeeruddin, Michaël Grätzel* Laboratory for Photonics and Interfaces (LPI) Swiss Federal Institute of Technology at Lausanne (EPFL) 1015 Lausanne, Switzerland *Email: michael.graetzel@epfl.ch For several decades, the need for an environmentally sustainable and ...

Research Essentials - Technical Library

Buffer pKa/pH Tables and Formulas: Useful pH Ranges of Selected Biological Buffers (25 °C, 0.1M) Trizma Buffer Table - pH vs. Temperature Phosphate Buffer Table - 0.2M solution Commonly Used Electrophoresis Buffers Helpful Formulas DNA / Protein Electrophoresis and Troubleshooting Tables: Effective ...

Restriction Site Positions and Functions

Click on the relevant restriction site to learn more about its function in the SnapFast plasmid system. AsiSI/SbfI | Bgl2 | NotI | HindIII | SacI | EcoRI | KpnI | NcoI | EcoRV | XhoI | XbaI BseRI | BsgI | ClaI | BamHI | StuI | NheI | SbfI | PacI | SwaI | FseI | AscI | PmeI All DNA sections are pre-s...

Recent Advances in Scalable Synthesis and Processing of Two-Dimensional Materials

1Department of Electrical and Systems Engineering, University of Pennsylvania, Philadelphia, Pennsylvania, 19104, USA 2School of Materials Science and Engineering, Nankai University, Tianjin, 300350, China 3Department of Materials Science and Engineering, Northwestern University, Evanston, Illinois,...

Downstream Applications for Plasmid DNA and Substrates

Extracted from Nucleic Acid Sample Preparation for Downstream Analyses, GE Healthcare, 2007 The purity of plasmid DNA in terms of protein, salt, particulates, RNA, and endotoxin contamination, and quality measures such as the percentage of supercoiled plasmid DNA, will all affect downstream function...

Optoelectronic Devices Based on Diketopyrrolopyrrole (DPP)-containing Conjugated Small Molecules

Jianhua Liu and Thuc-Quyen Nguyen* Center for Polymers and Organic Solids Department of Chemistry & Biochemistry University of California, Santa Barbara, California 93106, United States *Email: quyen@chem.ucsb.edu Optoelectronic devices such as light-emitting diodes (LEDs), solar cells, and light-em...

Troubleshooting for Molecular Cloning

Click here to return to the Molecular Biology Handbook Molecular cloning is used to assemble recombinant DNA molecules and to direct their replication within host organisms, making multiple DNA molecules. Cloning involves the replication of one molecule to produce a population of cells with identica...

Precision Genetic Modifications

Project Types Our scientists’ years of experience and Sigma-Aldrich’s access to multiple advanced gene editing technologies allow us to offer a cell engineering service with unparalleled success. We can engineer a variety of modifications in your cell line of choice to address your scientific questi...

Astec Chiral Chromatography Bibliography by Subject

Amino Acids, Peptides, Biomolecules Chemical, Environmental, Petrochemical Chiral LC-MS Food, Beverage, Natural Products Mechanistic Studies Pharmaceutical, Clinical Preparative, SFC, SMB Daniel W. Armstrong Publications Copyright restrictions do not permit us to provide copies of the articles liste...

Clone and Downstream Analysis - MISSION shRNA FAQs

What restriction enzymes are recommended for diagnostic digests of the clone DNA? We recommend Pvu II. Restriction digest with Pvu II results in three bands; however, clones with an additional Pvu II site in the hairpin insert will produce four bands: 2513 bp, 2325 bp, 1478 bp and 776 bp. The band s...

Ultra-High Efficiency Perovskite-Perovskite Tandem Solar Cells

Chemistry and Nanoscience Center, National Renewable Energy Laboratory, Golden, Colorado 80401, USA *Email: Kai.Zhu@nrel.gov Next generation solar cells have the potential to achieve conversion efficiencies beyond the Shockley-Queisser (S-Q) limit while also significantly lowering production costs. ...

Nucleic Acid Modifying Enzyme Selection Chart (Ultra Pure)

Find more protocols and selection guides in the Molecular Biology Guide. DNA Polymerases synthesize DNA from nucleotides. DNA polymerase is required for DNA replication, but also essential for many other activities in the cell, including genetic recombination, DNA repair, and reverse transcription. ...

Efficient Dye-Sensitized Solar Cells for Direct Conversion of Sunlight to Electricity

• • Download PDF • Kuppuswamy Kalyanasundaram Michael Grätzel Kuppuswamy Kalyanasundaram* and Michael Grätzel Laboratory for Photonics and Interfaces (LPI), Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland *Email: k.kalyanasundaram@epfl.ch Sunlight is an abundant natura...

Nanoparticle-based Zinc Oxide Electron Transport Layers for Printed Organic Photodetectors

Gerardo Hernandez-Sosa,1,2* Ralph Eckstein,1,2 Tobias Rödlmeier,1,2 Uli Lemmer1,2,3 1Lichttechnisches Institut, Karlsruher Institut für Technologie, Engesserstrasse 13, 76131 Karlsruhe, Germany 2InnovationLab, Speyerer Str. 4, Heidelberg, Germany 3Institut für Mikrostrukturtechnik, Karlsruher Instit...

Genome by Design

Without a robust understanding of what is being observed, no conclusions can be drawn. In biology, the multifaceted nature of even basic in vitro systems makes this a complex task, requiring not just stringent control of as many variables as possible, but also an appreciation of the likely impact of...