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801 matches found for separation

FLAG® System Literature

The FLAG Expression System is the proven and trusted method to express, purify and detect recombinant fusion proteins. Listed below are many references linked to PubMed, where you can find complete abstract information and relevant journal information. Tell us! We’re interested in knowing how our FL...

ANTI-FLAG® M2 Magnetic Beads | Recombinant Protein Purification & Detection

The FLAG Expression System is a proven method to express, purify and detect recombinant fusion proteins. We now offer a magnetic bead for immunoprecipitation, protein purification, and the study of protein-protein interactions. The ANTI-FLAG M2 Magnetic Bead is composed of murine derived, anti-FLAG ...

Small Molecules Drug Development

When analyzing small molecules the overarching need is to maximize sensitivity without compromising reproducibility and laboratory throughput. We have developed unique tools to maximize speed and selectivity throughout the entire workflow. Our tools support the requisite techniques, such as gas chro...

Trioctylmethylammonium thiosalicylate (TOMATS)

Aldrich ChemFiles 2006, 6.9, 13. A novel, high-performance, task-specific ionic liquid for the extraction of heavy metals from aqueous solutions Roland St. Kalb and Michael J. Kotschan, proionic Production of Ionic Substances GmbH, Leoben, Austria; www.proionic.at; info@proionic.at Task-specific ion...

Small Molecule Workflow

When analysing small molecules the overarching need is to maximize sensitivity without compromising reproducibility and laboratory throughput. We have developed unique tools to maximize speed and selectivity throughout the entire workflow. To support the diverse techniques required such as GC, spect...

Development of Small Molecule Donors for Solution-Processed Organic Solar Cells

Abby-Jo Payne and Gregory C. Welch Dalhousie University, Department of Chemistry 6274 Coburg Road, Halifax, Nova Scotia, Canada B3H 4R2 Email: gregory.welch@dal.ca Solution-processed organic photovoltaic devices (OPVs) have emerged as a promising clean energy generating technology due to their ease ...

Few Monolayer Atomic Layer Deposition (ALD) on Surfaces and Interfaces for Energy Applications

Department of Mechanical Engineering and Materials Science, One Brookings Drive, Washington University in St. Louis, St. Louis, MO 63130 *Email: parag.banerjee@wustl.edu Atomic layer deposition (ALD) exploits a sequential set of two or more surface reactions repeated cyclically to achieve thin film ...

Metabolomics Core Partnership Program Information Request

Sigma-Aldrich is proud to partner with select Core Facilities to offer the Metabolomics Core Partnership Program. The program provides tools and resources that enable breakthrough discoveries to be made more quickly, confidently and accurately. Sigma-Aldrich's scientific expertise and knowledge span...

DNA & RNA Purification GenElute Endotoxin-free Plasmid Kits

Description: Endotoxins (also known as lipopolysaccharides or LPS) are often co-purified with plasmid DNA and can significantly reduce transfection efficiencies in endotoxin-sensitive cell lines. The GenElute™ Endotoxin-free Plasmid Kits offer a simple, rapid, cost-effective method for purifying pla...

extra spindle pole bodies homolog 1 (S. cerevisiae)
Synonyms ESP1, SEPA
Species Human (9700) , Species Mouse (105988) , Species Rat (315330) , Species Zebrafish (797406) , Species dog (607879) , Species domestic cat (101084118) , Species cow (506740) , Species Horse (100063779) , Species chicken (425536) , Species sheep (101105898) , Species Domestic Rabbit (100356105) , Species naked mole-rat (101723260) , Species domestic guinea pig (100733255)
Summary: Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]

High Resolution Separation of Human Serum Albumin Tryptic Digest using Fused-Core HPLC Columns

Related Articles • Faster Protein and Peptide Liquid Chromatography: Introducing BIOshell Fused-Core HPLC Columns • Online SPE/LC-MS Method for the Rapid Analysis of Thyroid Hormones in Plasma Prof. Luigi Mondello University of Messina, Italy wayne.way@sial.com The last decades have witnessed the us...

MRT - Ring Closure — Synthesis of Methylenecyclopentane

Aldrich ChemFiles 2009, 9.4, 14. Benefit from MRT & Flow Chemistry: complete suppression of sidereactions. The cyclization of 1,5-hexadiene to exo-methylenecyclopentane (251356) poses multiple challenges: the batch reaction affords an inseperable mixture of the endo- and exo-cyclization product (Sch...

Proteomic analysis of membrane proteins

Related Links • Cell disruption and membrane preparation • Conditioning Purified Membrane Proteins • Performing a purification and on-column refolding of an insoluble histidine-tagged protein from a 100 ml E. coli culture using HisTrap FF 1 ml with ÄKTAprime plus • Performing a purity and homogeneity...

Column Packing and Preparation for Hydrophobic Interaction and Reversed Phased Chromatography

Prepacked columns from GE Healthcare will ensure reproducible results and the highest performance. Use small prepacked columns for media screening and method optimization to increase efficiency in method development, for example, HiTrap™ HIC Selection Kit, RESOURCE™ HIC Test Kit and RESOURCE™ RPC co...

FITC-labelled polysaccharides

FITC-Dextran FITC-DEAE-dextran (FDD) FITC-carboxymethyl-dextran (FCM-Dextran) FITC-polysucrose (FITC-Ficoll®) FITC-DEAE-polysucrose FITC-CM-polysucrose (FITC-CM-Ficoll®) FITC-inulin Fluorescein hyaluronic acid (FHA-Se) TRITC-labelled polysaccharides TRITC-polysucrose (TRITC-Ficoll®) Tetramethyl-rhod...

Protein AQUA® – Protein Biomarker Quantitation

Developed in collaboration with the Protein Quantitation Consortium, our AQUA Peptides enable accurate, efficient mass spectrometric quantitation of protein biomarkers. • Focus your analysis on significant protein biomarkers • Accurately quantitate low abundance proteins • Eliminate costly, time con...

Isolation of Plant and Fungal RNA from Challenging Samples using Norgen's Plant/Fungi RNA Purification Kit

Norgen’s Plant/Fungi Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA from a wide range of plant and filamentous fungal species. Total RNA can be purified from fresh or frozen plant tissues, plant cells or filamentous fungi samples using this kit. Al...

Kromasil HPLC Columns

Ordering Information Useful Links Literature Kromasil® premium silica-based HPLC columns and chromatography packings are developed for analytical up to process scale applications. Kromasil has superior mechanical and chemical stability, high available surface area, and a narrow pore size distributio...

Flexible and Printed Organic Thermoelectrics: Opportunities and Challenges

1 Department of Chemistry, Purdue University, 560 Oval Dr. West Lafayette, IN, 47907, USA. 2 The Organic Thermoelectric Laboratory, Materials Research Institute and School of Biological & Chemical Sciences, Queen Mary University of London, Mile End Road, London E1 4NS, United Kingdom. 3 Beijing Nati...

Vitamin D epi-Metabolites Separated with HybridSPE-Phospholipid

• • Download PDF • Related Articles • Method Optimization for LC-MS Analysis of Vitamin D Metabolite Critical Pairs in Serum • Troubleshooting Analyte Recovery when Using HybridSPE-Precipitation Technology • Minimizing Phospholipid Matrix Effects using HybridSPE-PL • Phospholipid Removal Using Hybri...

Improvement in LC/MS Analysis of Vitamin D Metabolites in Serum by Leveraging Column Selectivity and Effective Sample Prep

Craig Aurand, Senior Application Chemist, craig.aurand@sial.com Clinical interest in vitamin D stems from its implications in an ever-widening range of human health conditions.1 This heightened interest has spawned the need for analytical strategies to assess an individual’s vitamin D status. Becaus...

Metabolomics

Comprehensive Metabolomic resources, reagents and kits. We offer a large collection of Amino Acid Metabolites and Carbohydrate Metabolites. We carry enzymatic-based kits for the quantitation of nutrients and metabolites. Our alliance with the IUBMB enables us to feature the Nicholson Metabolic Pathw...

Frequently Asked Questions for Seppro® Products

1. What format is Seppro offered in? 2. What are the advantages of Seppro products? 3. What are the recommended usages for Seppro? 4. What types of projects are best fit for Seppro? 5. What are the immediate assays that can be used after processing samples using Seppro? 6. What is Sigma's Seppro lin...

Principles and Standard Conditions for Different Purification Techniques

Appendix 11, Extracted from Affinity Chromatography Vol. 2: Tagged Proteins, GE Healthcare, 2016 AC media separate proteins on the basis of a reversible interaction between a protein (or a group of proteins) and a specific ligand attached to a chromatographic matrix. The technique is well-suited for ...

HIC as a Polishing Step

Appendix 1, extracted from Hydrophobic Interaction and Reversed Phase Chromatography Principles and Methods (PDF), GE Healthcare, 2014 At the polishing stage of a purification protocol most impurities have been removed except for trace amounts or closely related substances such as structural variant...

Drugs & Pharmaceuticals - Chromatography and Sample Prep Literature from Supelco

Pub. Id. Subject Type Size (KB) No. of Pages T196901 Solid Phase Microextraction/Capillary GC Analysis of Drugs, Alcohols, and Organic Solvents in Biolog Bulletin 901 116 8 T198922 SPME/GC for Forensic Applications: Explosives, Fire Debris, and Drugs of Abuse Bulletin 922 235 8 T199925 SPME Applicat...

Natrix® Membrane Chromatography Technology

Next-level Downstream Productivity & Versatility – Experience our Natrix® Membrane Chromatography Technology The NatriFlo® HD-Q membrane is a porous polyacrylamide hydrogel containing a high density of pendant quaternary ammonium (Q) binding groups physically reinforced by an inert fiber web. The in...

Tissue Diagnostics Solutions

Discover our newly expanded and even more comprehensive product range for your tissue diagnostics workflow. We are dedicated to covering all your requirements and delivering quality in the fields of Histology, Cytology, Bacteriology and Hematology. From brilliant staining solutions and ready-to-use ...

Ascentis® Express U/HPLC Column Selection

The column selectivity has the highest influence on resolution in chromatography. Therefore, the selection of the best suitable column chemistry for the target analytes is an important selection parameter. C18 column chemistries are typically the first choice. Nevertheless, when a C18 doesn't give t...

Cell Signaling & Neuroscience Assay Kits by Name

A-B C D-G H-L M-O P R-T Product Code NAME Area of Interest Platform Size Sensitivity Species Description CS0740 Acid Phosphatase Assay Kit Nitric Oxide/ Cell Stress Enzymatic 1,000 in microwell plate 0.2 mUnit mammalian Quantitative Acid Phosphatase activity measurement in tissues, whole cell extrac...

Greener Alternatives Evaluation Matrix

Launch DOZN Tool Our approach to evaluating greener chemicals is based on the 12 Principles of Green Chemistry. We recognize that these principles distill into three major categories: improving resource use, more efficient use of energy, and minimizing human and environmental hazards. At present, we...