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    Search term:"separation"

    778 matches found for separation

    NMR Reference Standards

    Over the last decade, the utility of Nuclear Magnetic Resonance (NMR) spectroscopy has expanded. Traditionally, NMR spectroscopy was primarily a qualitative method used for structural identification. With the introduction of high field NMR instruments (600 MHz and higher), NMR is increasingly used i...

    Food & Beverage Analysis: Sugars & Saccharides

    Related Links • Carbohydrate Analytical Standards • apHera™ NH2 column • Ascentis® Express • SUPELCOGEL™ • SUPELCOGEL C-611 • Enzymatic Food Kits and Reagents Related Articles • Improved Analysis of Simple Sugars Using apHera™ NH2 HPLC Columns • Determination of Additives in Beverages Using Ascentis...

    Proteomics and Protein Expression Product Guide - Most comprehensive protein research offering available.:

    Product Guide Home Page Request Product Guides General Information (252 Kb PDF) Sample Preparation (4.89 Mb PDF) • Sample Prep Selection Guide • Extraction • Sample Fractionation & Enrichment • Post-Translation Modification • Organelle Enrichment • Additional Reagents Quantitation (479 Kb PDF) Expre...

    Proteomics Core Partnership Program Information Request

    Sigma-Aldrich is proud to partner with select Core Facilities to offer the MS Proteomics Core Partnership Program. The program provides tools and resources that enable breakthrough discoveries to be made more quickly, confidently and accurately. Sigma-Aldrich's scientific expertise and knowledge spa...

    Column Packing and Preparation

    Appendix 6, Extracted from Affinity Chromatography Vol. 2: Tagged Proteins, GE Healthcare, 2016 Prepacked columns from GE will ensure reproducible results and the highest performance. Use small prepacked columns for chromatography media scouting and method optimization and to increase efficiency in m...

    Cancer Research Techniques | Learning Center

    By focusing on analysis techniques and protocols specific for Cancer Research, Sigma-Aldrich is putting your research needs first. We will be featuring different cancer research tools throughout the year, providing you with the latest techniques and products necessary for the applications. Check bac...

    Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

    Extracted from Affinity Chromatography Vol. 1: Antibodies, GE Healthcare, 2016 Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step. GE o...

    Using the Strip Holder for Gel Rehydration

    IPGphor fixed-length Strip Holders allow IPG strips to be rehydrated and samples loaded in one step before proceeding automatically to perform the IEF separation. The IPG strips are 3 mm wide and 0.5 mm thick after rehydration. This protocol applies for both in-gel sample rehydration and sample appl...

    DNA & RNA Purification: mRNA and GenElute Direct mRNA Miniprep Kit

    Description Features and Benefits RNA Kits Kit Contents Preparation Time Comparison RNA Yield Specific mRNA Yield mRNA—Suitable for Northern Blots Description Procedures such as cDNA synthesis, expression profiling, and others, require separation of mRNA from the vastly more abundant rRNA and tRNA. ...

    extra spindle pole bodies homolog 1 (S. cerevisiae)
    Synonyms ESP1, SEPA
    Species Human (9700) , Species Mouse (105988) , Species Rat (315330) , Species Zebrafish (797406) , Species dog (607879) , Species domestic cat (101084118) , Species cow (506740) , Species Horse (100063779) , Species chicken (425536) , Species sheep (101105898) , Species Domestic Rabbit (100356105) , Species naked mole-rat (101723260) , Species domestic guinea pig (100733255)
    Summary: Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]

    Titania Nanotubes: Synthesis and Applications

    Eugen Panaitescu1 and Latika Menon2 Department of Physics Northeastern University, Boston, MA 02115 USA Email: 1e.panaitescu@neu.edu, 2l.menon@neu.edu Titania (titanium dioxide, TiO2) (Aldrich Prod. Nos. 799289, 204757, 232033, 248576, and 677469) is a versatile earth-abundant ceramic with many desi...

    Chemical Standards and Capillary Columns for US EPA Methods 608.1 and 608.2

    Related Articles • Dual-Column GC Analysis of Organochlorine Pesticides on SLB-5ms and SLB-35ms Katherine Stenerson Katherine.stenerson@sial.com US EPA Methods 608.1 and 608.2 are extensions of Method 608, and describe the extraction and analysis of certain organochlorine pesticides in municipal and...

    Enhanced Separation Capability in SIC using a Fused-Core® Column

    Enhanced Separation Capability in Sequential Injection Chromatography using an Ascentis® Express Column Based on Fused-Core® Particle Technology The following was generated with the assistance of an outside source using Sigma-Aldrich products. Technical content was generated and provided by: Petr Ch...

    When C18 is Not Quite Enough in HPLC...

    Denise Wallworth denise.wallworth@sial.com The automatic column choice in reversed phase HPLC is traditionally a C18 or perhaps a C8, but this does not always provide the optimum selectivity desired – it might not always have the best selectivity or the elution order that is required. Whether the co...

    Perfluorosulfonic Acid Membranes for Fuel Cell and Electrolyser Applications

    Deborah Jones Institut Charles Gerhardt CNRS–University of Montpellier, 34090 Montpellier, France Email: Deborah.Jones@univ-montp2.fr Introduction PFSA Polymer Types Composition, Structure, and Synthesis Routes PFSA Ionomer Dispersions Conductivity and Water Uptake Membrane Durability Better Membran...

    Research Essentials - Technical Library

    Buffer pKa/pH Tables and Formulas: Useful pH Ranges of Selected Biological Buffers (25 °C, 0.1M) Trizma Buffer Table - pH vs. Temperature Phosphate Buffer Table - 0.2M solution Commonly Used Electrophoresis Buffers Helpful Formulas DNA / Protein Electrophoresis and Troubleshooting Tables: Effective ...

    MOPS Specification Comparison

    Synonym: 3-Morpholinopropane-1-sulfonic acid, 3-(N-Morpholino)propanesulfonic acid and 3-Morpholinopropanesulfonic acid The zwitterionic buffer MOPS is a structural analog to MES, one of the Good's buffers that were developed for general use in biochemistry. MOPS is primarily used as a buffer for th...

    Non-Organic-Based Isolation of Plant microRNA using Norgen's Plant/Fungi RNA Purification Kit

    Small RNAs, including microRNAs (miRNAs) and short interfering RNAs (siRNAs), are key components of an evolutionarily conserved system of RNA-based gene regulation in eukaryotes. In eukaryotes, regulatory small RNAs are divided into two main classes; (a) small interfering RNAs (siRNAs) are double-st...

    MSMLSDiscovery™ Software Manual

    MSMLSDiscovery™ is a software tool designed to streamline and simplify the tedious process of building authentic standard libraries for mass spectrometry. The program is tailored to work with the Mass Spectrometry Metabolite Library of Standards (MSMLS) distributed by IROA technologies. Future relea...

    Adsorbents for use as Purification Media

    Sigma-Aldrich's polymeric resins portfolio is further complemented by a broad range of inorganic adsorption media, for a wide variety of purification applications. Our offering of inorganic adsorption media includes: • Bare silica • Silica, bonded, e.g. C18, C8, aminopropyl, chloropropyl, etc. • Lip...

    Bioanalytical and Biotech Applications

    We offer a range of solvents suitable for detection, isolation, purification, separation & quantitation of biomolecules. For more information on the broader range of reagents, kits and more, please visit our bioanalytical overview. Synthesis and Cell Culture Cell Culture DNA/RNA Purification Oligonu...

    Class-Selective Enantiomeric Separation of beta-Androgenic Receptors using CHIROBIOTIC™ T Stationary Phase

    Related Articles • Fast Extraction and Low Level Detection of Chloramphenicol in Shrimp • Online SPE/LC-MS Method for the Rapid Analysis of Thyroid Hormones in Plasma Daniel Shollenberger daniel.shollenberger@sial.com The inherent chirality of bio-organic molecules and its affect on biological activ...

    SAFC Arklow Facility Focus

    SAFC-Arklow, Ireland is the company’s primary center for cGMP production of commercial and late-stage APIs and Advanced Intermediates. The 64,000 m2 complex supports customers with expertise in new process evaluation, process development validation and technology transfer. The site is also equipped ...

    Electrospinning: An Enabling Technique for Nanostructured Materials

    • • Download PDF • Dr. Jingwei Xie and Prof. Younan Xia Department of Biomedical Engineering, Washington University, St. Louis, MO Fibrous nanomaterials are attractive for a range of applications due to their intrinsically high porosities and large surface areas. Electrospinning is a simple, versati...

    How to make and use gradients of Percoll

    Extracted from Cell Separation Media Methodology and Applications, GE Healthcare, 2007 In order to use Percoll to prepare a gradient, the osmolality of Percoll (undiluted) must first be adjusted with saline or cell culture medium to make Percoll isotonic with physiological salt solutions. Adding 9 p...

    Perovskite-phase Metal Oxide Nanostructures: Synthesis, Properties, and Applications

    Yuanbing Mao1, Hongjun Zhou1, and Stanislaus S. Wong1,2,* 1Department of Chemistry, State University of New York at Stony Brook, Stony Brook, NY 11794-3400 *Email: sswong@notes.cc.sunysb.edu 2Condensed Matter Physics and Materials Sciences Department, Brookhaven National Laboratory, Upton, NY 11973 ...

    Importance of TFF for concentration

    Filtration is a pressure-driven separation process that uses membranes to separate components in a liquid solution or suspension based on their size and charge differences. The two kinds of filtration are tangential flow filtration and normal flow filtration. In Normal Flow Filtration (NFF), fluid i...

    Preparation and Use of an Integrated Microcapillary HPLC Column and ESI Device for Proteomic Analysis

    Sigma® has prepared product lists for commonly used protocols from Cold Spring Harbor Protocols. View the entire protocol on BioSupplyNet.com. Selected Protocol from "Proteins and Proteomics: A Laboratory Manual", David R. Goodlett, Eugene C. Yi, and Philippe Mottay. Preparation and Use of an Integr...

    Optimizing Purification of Histidine-Tagged Proteins

    Chapter 4, Extracted from Affinity Chromatography Vol. 2: Tagged Proteins, GE Healthcare, 2016 Three methods for optimizing purification of histidine-tagged proteins to achieve high purity are discussed in this chapter: • Optimizing using imidazole • Optimizing using different metal ions • Optimizing...

    DNA / Protein Electrophoresis and Troubleshooting Tables

    • Effective Range of Separation of DNA in Polyacrylamide Gels • Effective Range of Separation of DNA in Agarose Gels • DNA Size Migration of Sample Loading Dyes • Troubleshooting Guide for SDS-PAGE Protein Electrophoresis % Acrylamide (w/v)1 Efficient Range of Separation (bp) 3.5 1000-2000 5.0 80-50...

    Cell Signaling & Neuroscience Assay Kits by Name

    A-B C D-G H-L M-O P R-T Product Code NAME Area of Interest Platform Size Sensitivity Species Description CS0740 Acid Phosphatase Assay Kit Nitric Oxide/ Cell Stress Enzymatic 1,000 in microwell plate 0.2 mUnit mammalian Quantitative Acid Phosphatase activity measurement in tissues, whole cell extrac...