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    Search term:"separation"

    665 matches found for separation

    Silylation Derivatization Reagent

    Silylation is the most widely used derivatization technique. Nearly all functional groups which present a problem in gas chromatogaphic separation (hydroxyl, carboxylic acid, amine, thiol, phosphate) can be derivatized by silylation reagents. It involves the replacement of an acidic hydrogen on the ...

    Astec CHIROBIOTIC™ R Chiral HPLC Columns

    Astec Products | Technical Resources Ordering Information Features • Covalently bonded glycopeptide - Ristocetin A • Multi-modal - operates in normal, reversed and polar organic phase • Targets anionic chiral molecules • Complementary to CHIROBIOTIC V and CHIROBIOTIC T CHIROBIOTIC R is a new introdu...

    Custom DNA Oligos - Purity Verification

    Related Articles • Quality Control, Quality Assurance • Critical Raw Materials • Synthesis and Processing • QC Analysis by Mass Spectrometry • UV Quantification • Custom Formats • Post Packaging Inspection • Quality Registrations In many cases oligonucleotides require quantitative analysis to verify...

    Oligos: Frequently Asked Questions (FAQs)

    • How does Sigma quantitate oligos? • I ordered a 0.2 µmole scale oligo. Does this mean that I will receive 0.2 µmole of product? • What oligonucleotide modifications does Sigma offer? • I received my oligonucleotide but there seems to be nothing in the vial. Where is the oligonucleotide? • What pur...

    Headspace SPME GC/MS Analysis of Terpenes in Cannabis

    Related Content • Improved Recoveries for GC/MS/MS Analysis of Pesticide Residues in Cannabis Related Articles • Reporter US Volume 34.4 • Robustness of the Discovery® HS F5 HPLC Column in the Study of Metabolomics • Reaching the “Peak” of Recovery: Improving Antibody Separations with the Inclusion ...

    Analysis of NBOMe Hallucinogens from Urine

    Related Articles • Reporter US Volume 33.3 (4 Mb PDF) • LC-MS of Illicit Bath Salts in Urine • Utilizing Novel SPME LC Tips for Forensic Screening of Drugs of Abuse in Urine Samples • SPME in the Analysis of Illicit Drugs • Accurate Biological Testing for Amphetamine and Methamphetamine Abuse Using ...

    Solvents for Preparative Chromatography Prepsolv®

    Solvents Optimized for Preparative Chromatography Solvents used in preparative chromatography applications not only have to support optimal purity and speed of separation. They also need to minimize wear on your valuable equipment, and they must be usable efficiently and securely in larger quantitie...

    Astec CHIROBIOTIC™ Chiral HPLC Column Literature References

    Astec Products | Technical Resources 1. Macrocyclic Antibiotics as a New Class of Chiral Selectors for Liquid Chromatography, D. W. Armstrong, Y. Tang, S. Chen. Y. Zhou, C. Bagwell, J-R. Chen, Analytical Chemistry, Vol. 66, No. 9, 1473-1484 (1994). 2. A Covalently Bonded Teicoplanin Chiral Stationar...

    Distillation Adapter for On-The-Fly Sampling

    Paula Freemantle Product Manager labware@sial.com back to top Distillation is the most widely used bulk separation method used in the laboratory as well as industry. Beyond purifi cation, it is widely used to characterize complex fl uids (such as fuels) through measurement of the distillation curve,...

    extra spindle pole bodies homolog 1 (S. cerevisiae)
    Synonyms ESP1, SEPA
    Species Human (9700) , Species Mouse (105988) , Species Rat (315330) , Species Zebrafish (797406) , Species dog (607879) , Species domestic cat (101084118) , Species cow (506740) , Species Horse (100063779) , Species chicken (425536) , Species sheep (101105898) , Species Domestic Rabbit (100356105) , Species naked mole-rat (101723260) , Species domestic guinea pig (100733255)
    Summary: Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]

    New Conducting and Semiconducting Polymers for Plastic Electronics

    Related eTool • OLED eFabricator • • Download PDF • TDA Research, Inc. In the emerging field of organic printable electronics, such as OLEDs and organic photovoltaics (OPVs), there is a significant need for improved organic conducting and semiconducting materials. This paper reports our recent progr...

    Applications for Peptide ES-C18 Columns

    A Breakthrough in Bioseparations Performance Applications - Peptides, Peptide Mapping, Small Proteins Ordering Information Peptide ES-C18 brochure (900 Kb PDF) Learn more about the Fused-Core Advantage Ascentis Express Peptide ES-C18 is a high-speed, high-performance liquid chromatography column bas...

    Electrospinning: An Enabling Technique for Nanostructured Materials

    • • Download PDF • Dr. Jingwei Xie and Prof. Younan Xia Department of Biomedical Engineering, Washington University, St. Louis, MO Fibrous nanomaterials are attractive for a range of applications due to their intrinsically high porosities and large surface areas. Electrospinning is a simple, versati...

    Selectivity of Peptides on Amide Embedded Polar Group Phases Versus Alkyl Phases

    Hillel Brandes hbrandes@sial.com and Patrick Myers pmyers@sial.com It has been known that small molecule analytes with hydrogen bond donor moieties display enhanced retention on embeddedpolar group (EPG) phases(1, 2, 3). However, no such systematic study has been done with peptide analytes, until th...

    Isolation of Plant and Fungal RNA from Challenging Samples using Norgen's Plant/Fungi RNA Purification Kit

    Norgen’s Plant/Fungi Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA from a wide range of plant and filamentous fungal species. Total RNA can be purified from fresh or frozen plant tissues, plant cells or filamentous fungi samples using this kit. Al...

    TRI Reagent® Protocol

    Product No. T9424 Sample Preparation 1A. Tissue: Homogenize tissue samples in TRI Reagent (1 ml per 50–100 mg of tissue) in a Polytron® or other appropriate homogenizer. Note: If minimal shearing of the DNA is desired, use a loosely fitting homogenizer, not a Polytron (see DNA Isolation, step 3, not...

    MRT - Ring Closure — Synthesis of Methylenecyclopentane

    Aldrich ChemFiles 2009, 9.4, 14. Benefit from MRT & Flow Chemistry: complete suppression of sidereactions. The cyclization of 1,5-hexadiene to exo-methylenecyclopentane (251356) poses multiple challenges: the batch reaction affords an inseperable mixture of the endo- and exo-cyclization product (Sch...

    Introduction to SPME [VIDEO]

    Supelco solid phase microextraction or SPME is a unique solventless sample prep technique. SPME uses a polymer-coated fiber to concentrate volatile and semi-volatile organics in one extraction step. SPME requires no solvents or complicated extraction apparatus. The SPME fiber is attached to a plunge...

    Atto Dyes for Superior Fluorescent Imaging

    • Alternatives to common Fluorophores • Reactive Atto Dyes • Atto Dye Conjugates Activated fluorescent dyes are routinely used to tag proteins, nucleic acids, and other biomolecules for use in life science applications including fluorescence microscopy, flow cytometry, fluorescence in situ hybridiza...

    Derivatization and Separation of Aliphatic Amines

    Hugh Cramer, Associate Scientist Shyam Verma, Market Segment Manager Michael Kiselewsky, Product Manager The aliphatic amines are commonly analyzed in environment and biological samples.1 Detection and separation of aliphatic amines by HPLC has been reported2,3,4 earlier. However, aliphatic amines, ...

    Chiral GC Analysis of Carvone Enantiomers in Essential Oils

    Using Astec® CHIRALDEX® G-TA Capillary GC Columns D-Carvone and L-carvone are enantiomeric compounds that have very different smells. These mirror image compounds provide very different aroma notes. D-Carvone is the major compound found in caraway seed essential oil, and a large component of dill se...

    Selection Guide for Ascentis® Express HPLC Columns

    Ascentis Express C18 is the first choice for starting a new method. However, when a C18 doesn't give the desired separation or your sample contains compounds that are known to be difficult to retain or resolve on a C18, consider changing stationary phases. The range of selectivity provided by Ascent...

    TAPS Specification Comparison

    Synonym: N-[Tris(hydroxymethyl)methyl]-3-aminopropanesulfonic acid, [(2-Hydroxy-1,1-bis(hydroxymethyl)ethyl)amino]-1-propanesulfonic acid TAPS is a zwitterionic buffer used in biochemistry and molecular biology. TAPS is utilized in capillary electrophoresis of such materials as DNA and DNA-dye compl...

    Columns and Analytical Standards for SEC of Protein Samples

    Aqueous size-exclusion chromatography (SEC) is well established for protein characterization. As the technique has increasingly been used for quality control of biologics since the early days of recombinant protein development for pharmaceutical purposes, various method improvements have evolved. To...

    Recent Advances in Hybrid Halide Perovskites-based Solar Cells

    Kuppuswamy Kalyanasundaram, Shaik. M. Zakeeruddin, Michaël Grätzel* Laboratory for Photonics and Interfaces (LPI) Swiss Federal Institute of Technology at Lausanne (EPFL) 1015 Lausanne, Switzerland *Email: michael.graetzel@epfl.ch For several decades, the need for an environmentally sustainable and ...

    Narrow Bore GFC Columns for Protein Analysis

    Traditionally, in Gel Filtration Chromatography (GFC), the column diameter has been larger than the diameter of HPLC columns used in interactive modes of liquid chromatography. The main reason for this was that HPLC instruments were optimized for 4.6 mm I.D. columns that were 25 cm long. To prevent ...

    Size Exclusion Chromatography Principles and Methods

    Extracted from Size Exclusion Chromatography Principles and Methods, (PDF) GE Healthcare, 2014 • Column Packing and Preparation for Size Exclusion Chromatography • Packing a Column and Performing a Separation with Sephadex® LH-20 • Performing a Separation with Sephacryl® • Performing a Separation wi...

    Summer 02 Proteomics

    A TECHNICAL APPLICATION NEWSLETTER VOL 3 ISSUE 2 Summer 2002 Proteomics Proteomics: New Products for Protein Analysis By Staci Rohn Sigma-Aldrich Corporation, St. Louis, MO, USA The Human Genome Project, one of the most widely heralded projects in science, has provided a great deal of data about the...

    Determination of L- and D-Lactic Acid Enantiomers by HPLC-MS-MS

    Sensitive Determination of L- and D-Lactic Acid Enantiomers by HPLC-MS-MS The following was generated with the assistance of an outside source using Sigma-Aldrich products. Technical content was generated and provided by: H. Henry1, N. Marmy Conus2, P. Steenhout2, A. Benguin1, and O. Boulat1 1 Clini...

    Separation of Protein Isoforms from Different Species Using Discovery BIO C5

    Denise Wallworth denise.wallworth@sial.com Cytochrome c is a small (ca 12 kDa) mitochondrial protein found in plants, animals and many unicellular organisms, where it is an essential component of the electron transport chain. This protein is present in many closely related variants that can be used ...

    Titania Nanotubes: Synthesis and Applications

    Eugen Panaitescu1 and Latika Menon2 Department of Physics Northeastern University, Boston, MA 02115 USA Email: 1e.panaitescu@neu.edu, 2l.menon@neu.edu Titania (titanium dioxide, TiO2) (Aldrich Prod. Nos. 799289, 204757, 232033, 248576, and 677469) is a versatile earth-abundant ceramic with many desi...