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Successful Amplification of Thermally Degraded DNA Using PCRboost™

Introduction DNA samples are often exposed to harsh environmental conditions, including heat, humidity, UV or chemicals. Some procedures, such as the extraction of samples from formalin-fixed paraffin embedded tissue samples, subject DNA to multiple extreme conditions. The common end result of expos...

Analysis of Human Plasma Lipids Using sub-2 μm C18 UHPLC Column with MS Detection

Related Articles • Reporter US Volume 34.4 • Robustness of the Discovery® HS F5 HPLC Column in the Study of Metabolomics • Reaching the “Peak” of Recovery: Improving Antibody Separations with the Inclusion of Organic Alcohols • Rapid Determination of Protein Binding Affinity Using Solid Phase Microe...

dNTP Mediated Hot Start PCR Protocol

Hot Start dNTPs are modified with a thermolabile protecting group at the 3’ terminus. The presence of this modification blocks nucleotide incorporation by DNA polymerase until the nucleotide protecting group is removed during a heat activation step. When standard cycling protocols are employed, a 0–...

Everolimus-d4: An Internal Standard for Quantitation of Everolimus and Related Immunosuppressants by LCMS

Everloimus-d4 was synthesized and certified for use as an internal standard in LCMS applications. Use of the standard in LCMS applications was demonstrated by quantitation of an everolimus control sample against a calibration curve. The synthesis was developed from rapamycin by analogy to published ...

Thermal Transitions of Homopolymers

Literature values for the glass transition temperature, (Tg), and melting temperature, (Tm) for the more common homopolymers are listed in the table below. Polymers are listed by the repeating unit in the polymer chain. The polymers and corresponding monomers listed are available from us. Literature...

KAPA2G Fast PCR Kits FAQ

What are the recommended applications for KAPA2G Fast PCR Kits? • Standard, end-point PCR assays normally performed with wild-type Taq (or hot-start formulations thereof) – but completed in 20%–70% less time • High-throughput end-point PCR • End-point PCR for genetic testing or downstream Sanger seq...

FITC-labelled polysaccharides

FITC-Dextran FITC-DEAE-dextran (FDD) FITC-carboxymethyl-dextran (FCM-Dextran) FITC-polysucrose (FITC-Ficoll®) FITC-DEAE-polysucrose FITC-CM-polysucrose (FITC-CM-Ficoll®) FITC-inulin Fluorescein hyaluronic acid (FHA-Se) TRITC-labelled polysaccharides TRITC-polysucrose (TRITC-Ficoll®) Tetramethyl-rhod...

Evaluation of DNA Extraction and Amplification Protocols to Investigate Bacterial Communities on Cultural Heritage

1A. Meloscia, M. Orsini, 1G. Lustrato, and 1G. Ranalli 1DISTAAM, Università degli Studi del Molise via De Sanctis, 86100 Campobasso (I) Correspondence to: Giancarlo Ranalli, DISTAAM, Università degli Studi del Molise via De Sanctis, 86100 Campobasso, Italy. Tel. +39-0874-404604 (fax –652). E-mail: r...

dPCR Protocol

dPCR is a relatively new technology and each platform and application has specific requirements (see Digital PCR for further details). The protocols are specific for each system and are provided, with excellent support, by the instrument manufacturers. Therefore, the information provided below is a ...

Aminoglycoside Analysis in Pork Muscle using Molecularly Imprinted Polymer Cleanup and LC/MS/MS Detection

Aminoglycosides are a well-known class of antibiotics that are routinely monitored in animal-derived foods. Many countries have instituted regulatory limits for aminoglycosides. There is growing concern about the impact these microbial resistant compounds have on human health and development. A sens...

Frequently Asked Questions - radiello® Products

On this page: • General • Diffusive Bodies • RAD170 for Hydrogen Sulfide • RAD145 for BTEX/VOCs • RAD130 for BTEX/VOCs See Also: • What is passive diffusive sampling? • What is radiello®? • How to use radiello® • Applications What is the purpose of the recommended extra storage tubes? The extra stor...

Metabolic Pathways ATP Synthase Animation Series

View ATP Synthase Animation The Metabolic Pathways Animation series requires the Adobe Flash Plug-in for proper viewing. In 1997 the Nobel Prize in chemistry was awarded to Professor Paul D. Boyer, University of California, Los Angeles, USA, and Dr. John E. Walker, Medical Research Council Laborator...

KAPA2G Robust PCR Kits FAQs

What are the recommended applications for KAPA2G Robust PCR Kits? • Amplification of templates with high GC or AT content • Amplification of templates containing common PCR inhibitors at levels inhibitory to wild-type Taq • Amplification from crude samples, e.g. buccal swabs; cultured mammalian, bac...

How to Choose a Capillary GC Column

An optimized chromatographic separation begins with the column. The selection of the proper capillary column for any application should be based on four significant factors: stationary phase, column I.D., film thickness, and column length. The practical effects of these factors on the performance of...

The Samplicity Filtration System Streamlines Chromatography Sample Preparation

Recent innovations that have accelerated chromatographic separation, such as ultrahigh performance liquid chromatography [UHPLC] and monolithic chromatography columns, are turning up the pressure on sample preparation processes to keep pace. It is important that analytical researchers choose a sampl...

Featured article on Peroxide–forming Solvents used in research offered by Sigma-Aldrich.

A significant number of laboratory solvents can undergo autoxidation under normal storage conditions to form unstable and potentially dangerous peroxide by-products. This process is catalyzed by light and heat and occurs when susceptible materials are exposed to atmospheric oxygen. Molecular structu...

High Performance Liquid Chromatography (HPLC & UHPLC)

We offer a wide selection of high performance liquid chromatography (HPLC, UHPLC, and LC/MS) columns for small and large molecule (biomolecule) analysis, including those employing totally porous particle (TPP) with our Purospher® STAR columns, superficially porous particle (SPP) such as Ascentis® Ex...

Analysis of Bisphenol A in Broth-Based Soup Matrices

Related Products • SupelMIP SPE Bisphenol A Related Articles • Reporter US Volume 33.3 (4 Mb PDF) • Determination of Bisphenol A in Drinking Water • SPME-GC-MS Determination of Phthalate Esters in Ramen Noodle Flavor Packets The use of bisphenol A (BPA)-based plastics in food and beverage containers...

Phenolic Calibration Standards for Monitoring Wastewaters

By Vicki Yearick, Environmental Market Segment Manager … vyearick@sial.com Phenolic compounds are important raw materials for the manufacture of plastic resins, pharmaceuticals, laboratory reagents, dyes, germicidal paints and general disinfectants. Because of the toxicity of many phenolics to both ...

ATRP: Complete Tools for the Synthesis of Well-Defined Functionalized Polymers

• Synthesis of functional polymers via ATRP • Functional initiators for ATRP • Ligands for highly active ATRP catalysts • Synthetic protocol: Example of ICAR ATRP • Summary • Products for ATRP Atom transfer radical polymerization (ATRP) provides a simple route to many well-defined (co)polymers with ...

AptaTaq Fast PCR Master Protocol & Troubleshooting

Product No. ATPAM-RO Procedure 1. Thaw primer and nucleic acid template solution; mix by vortexing. 2. Prepare PCR primer solutions (e.g., in a concentration of 10 µM for each primer). 3. Vortex the AptaTaq Fast PCR Master (vial 1). 4. Spin down all vials in a microcentrifuge prior to opening to ens...

KAPA3G Plant PCR Kits FAQs

What are the recommended applications for KAPA3G Plant PCR Kits? • Direct PCR from crude plant samples including leaf tissue and seed • Crude PCR from leaf tissue and seeds • Successful amplification of CTAB-extracted DNA • Streamlined workflows for transgenic screening • As a solution for improving...

Chromatofocusing: Principles and Methods

Related Links • Sample Preparation in Ion Exchange Chromatography • Non-volatile and Volatile Buffer Systems • Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing • Selection of Purification Equipment • Column Cleaning for Ion Exchange Chromatography and Chromatofocusin...

Chiral GC Analysis of Carvone Enantiomers in Essential Oils

Using Astec® CHIRALDEX® G-TA Capillary GC Columns D-Carvone and L-carvone are enantiomeric compounds that have very different smells. These mirror image compounds provide very different aroma notes. D-Carvone is the major compound found in caraway seed essential oil, and a large component of dill se...

Extract-N-Amp Reagent Provides a Highly Effective Way of Generating PCR Products from FTA® Blood Cards

Mairi J. Hunter, The Sequencing Service, MSI/WTB Complex, University of Dundee, Dundee, Scotland Iain McLaren-Lee and Nicholas R. Helps, Sigma-Aldrich Corp., St. Louis, MO, USA Extract-N-Amp is a versatile, combined DNA extraction and amplification kit intended to simplify the generation of PCR prod...

Next Generation Cell Free Protein Expression Kit (Wheat Germ) (CFPS700) PROTOCOL

Introduction Primer Design Production of Transcription Template Transcription Translation Protein synthesis using cell-free protein synthesis reagent kit consists of three stages: preparation of transcription template, transcription and translation. For the use of the translation reagent set, please...

Real-time Quantitative Representation Measurement of Eighty Loci Using GenomePlex® Whole Genome Amplification

By Brian Buntaine, Deborah Vassar, and Ernest Mueller Sigma-Aldrich Corporation, St. Louis, MO, USA The methodologies of molecular biology have opened many fields of scientific research, allowing scientists to access the vast wealth of genetic information found in DNA. As these techniques have becom...

qPCR Efficiency Determination Protocol

Once an assay has been optimized, it is important to verify the reaction efficiency. This information is important when reporting and comparing assays. In this example protocol, the assay efficiency is compared over a wide and narrow dynamic range of cDNA concentrations. In practice, it is common to...

Amplification of Damaged DNA with Restorase DNA Polymerase (R1028)

• Background • Reagents • Preparation Instructions • Storage and Stability • Procedure • Primer Hot Start • Materials • Troubleshooting Guide • References Restorase® DNA Polymerase with 10x Reaction Buffer combines Sigma's Long and Accurate enzyme technology with a DNA repair enzyme resulting in a b...

State-of-the-art two-dimensional gel electrophoresis: a key tool of proteomics research

Nature Protocols Volume 1 Issue 2, 20 August 2006, Pages 812 - 823 doi: 10.1038/nprot.2006.104 Nature Publishing Group Odile Carrette a , Pierre R Burkhard a, b , Jean-Charles Sanchez a , Denis F Hochstrasser a, c, d a Biomedical Proteomics Research Group, Department of Structural Biology and Bioinf...