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    Search term:"sonication"

    55 matches found for sonication

    Solid Phase Extraction: Normal Phase Methodology

    Basic Steps Common Normal-Phase Solvents In order for polar retention to occur between the sorbent and the sample, the analyte must be introduced to the SPE device in a non-polar sample or mobile phase environment. Therefore, typical sample matrices that can be employed in normal-phase SPE include h...

    Tools for improving yields and purity from recombinant bacterial proteins [WEBINAR]

    Various methods for the extraction and purification of recombinant proteins from bacteria are in use and have been well described. Common methods for cell lysis involve mechanical disaggregation of cells, such as sonication, but this can be time-consuming when working with multiple samples and more ...

    ChIP Troubleshooting Questions

    • On page 3 of the bulletin, the tissue prep procedure calls for making the cross-link solution with formaldehyde in cell culture medium but there is no mention of cell culture medium anywhere else and why would this be used instead of an extraction buffer? What else can be used? • What if there are...

    Chromatin Immunoprecipitation Kit CHP1 Protocol

    Product Description Storage/Stability Workflow Preparation Instructions Procedure Analysis Troubleshooting Guide Frequently Asked Questions Materials References Poster & Application Note Protein-DNA interactions play critical roles in cellular functions including signal transduction, gene transcript...

    Recycling Micro-Assay of β-NAD and β-NADH

    • • Download PDF • 1. OBJECTIVE To standardize a procedure for the recycling micro-assay of β-NAD and β-NADH at Sigma-Aldrich Saint Louis. 2. SCOPE This procedure applies to products that have a specification for the recycling micro-assay of β-NAD and β-NADH. 3. DEFINITIONS 3.1. Purified Water = wat...

    SeqPlex RNA Amplification Kit Protocol

    The SeqPlex RNA Amplification kit provides a method for amplification of total RNA or isolated mRNA prior to entry into the workflows of the commonly used deep sequencing platforms. Microgram quantities of double-stranded cDNA are generated from low nanogram to picogram quantities of total RNA in ei...

    Enzymatic Assay of Lyticase

    This procedure may be used for the determination of Lyticase activity using Baker’s yeast as the substrate. The turbidimetric rate determination [Absorbance at 800 nm (A800), Light path = 1 cm] is based on the following reaction: Unit Definition: One unit of lyticase will produce a ΔA800 of 0.001 pe...

    CoMoCAT® Single-wall Carbon Nanotubes

    Related Products • Carbon Nanomaterials • Graphene Technologies • Nanomaterials Resources Related Articles • Carbon Nanomaterials: Elemental Analyses and Quantification of Their Accumulation in Living Cells • Boron Nitride Nanotubes: Properties, Synthesis and Applications • Novel Properties and Appl...

    Separation of Ginsenosides in Panax Quinquefolius (American Ginseng)

    Related Information • Analytix 3, 2015 (PDF) Belonging to the plant genus panax, ginseng is one of the most well-known herbal medicines. It has been used in traditional Chinese medicine for centuries, but has also become very popular in the Western world. Medicines derived from the ginseng root are ...

    sonic hedgehog
    Synonyms HHG1, HLP3, HPE3, MCOPCB5, SMMCI, TPT, TPTPS
    Species Human (6469) , Species Mouse (20423) , Species Rat (29499) , Species chicken (395615) , Species domestic cat (100169727) , Species dog (608860) , Species cow (286821) , Species sheep (780492) , Species sheep (101117666) , Species domestic guinea pig (100732014) , Species naked mole-rat (101719896) , Species Domestic Rabbit (100352774)
    Summary: This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities. Additionally, mutations in a long range enhancer located approximately 1 megabase upstream of this gene disrupt limb patterning and can result in preaxial polydactyly. [provided by RefSeq, Jul 2008]

    Summer 02 HIS-Select

    A TECHNICAL APPLICATION NEWSLETTER VOL 3 ISSUE 2 Summer 2002 Molecular Biology Application Note HIS-SelectTM Affinity Gels and Plates for Affinity Capture of Histidine-Containing Proteins By Ned Watson, Patricia Lindbloom*, Kelly Foster, Jodi Zobrist, Michael Scott, Rick Mehigh and John Dapron Sigma...

    Chromatin Isolation by RNA Purification (ChIRP) Protocol

    1. Probe Design 1.1 Design anti-sense oligo probes tiling the ncRNA of interest using the free online Biosearch Technologies' Stellaris FISH Probe Designer 1.2 Probe design parameters: Tile the entire ncRNA from 5’ to 3’; one 20-mer probe every 100 bp of RNA; spacing between probes = 80; target GC% ...

    What is Hyaluronan?

    • Brief Summary • Nomenclature • Chemistry and Physical Properties • Biological Roles • Degradation of Hyaluronan • Hyaluronan Synthesis • Glycosil vs. Unmodified HA • History of Hyaluronan • References Brief Summary Hyaluronic acid (HA) is the simplest glycosaminoglycan (a class of negatively charg...

    SeqPlex Enhanced DNA Amplification Kit Protocol

    Product Description Workflow Reagents Provided Storage/Stability Procedure Appendix 1: Optional Primer Removal Assay Troubleshooting Guide FAQs References The SeqPlex DNA Amplification Kit (SEQXE) for whole genome amplification (WGA) is designed to facilitate next-generation sequencing (NGS) from ex...

    Manufacturing, Characterization and Use of Single Walled Carbon Nanotubes

    • • Download PDF • SouthWest NanoTechnologies, Inc. 2501 Technology Place Norman, OK 73071 *Email: pwallis@swentnano.com Carbon nanotubes are materials that possess remarkable properties and offer extraordinary possibilities. This article gives a brief overview of the physico-chemical nature and cha...

    Bacterial Cell Lysis

    CelLytic products are a family of protein extraction reagents specifically formulated to lyse and extract cellular proteins based on the type of expression system. CelLytic proprietary formulations for bacterial cell lysis are available in a range of products specific to your protein extraction need...

    Western Blotting Sample Preparation

    Chapter 2, Extracted from Western Blotting Principles and Methods, GE Healthcare Life Science, 2011 Symbols This symbol indicates general advice on how to improve procedures or recommends measures to take in specific situations ** indicates a third party trademark The importance of good sample prepa...

    Single-Walled Carbon Nanotubes: Exfoliation and Debundling Procedure

    The dispersibility and bundle defoliation of single-walled carbon nanotubes (SWCNTs), which can be applied to materials produced by the CoMoCAT® process, have been extensively investigated by SouthWest NanoTechnologies (SWeNT ®) and at the University of Oklahoma.1 The procedure employed to disperse ...

    Probes for 19F Magnetic Resonance Imaging: PERFECTA

    PERFECTA is not available in the US. 19F Magnetic Resonance Imaging (19F MRI) is gaining momentum as a powerful diagnostic modality among currently available noninvasive bioimaging techniques.1 The absence of endogenous organic 19F signals in living tissues allows for in vivo visualization of exogen...

    Sera-Mag™ and Sera-Mag SpeedBeads Magnetic Particles

    Introduction Sera-Mag Magnetic SpeedBeads Sera-Mag Magnetic Particles Materials Sera-Mag and Sera-Mag SpeedBeads provide cost effective magnetic bead separation technology for molecular biology applications, nucleic acid isolation, and research immunoassays. Sera-Mag superparamagnetic particles comb...

    Frequently Asked Questions - radiello® Products

    On this page: • General • Diffusive Bodies • RAD170 for Hydrogen Sulfide • RAD145 for BTEX/VOCs • RAD130 for BTEX/VOCs See Also: • What is passive diffusive sampling? • What is radiello®? • How to use radiello® • Applications What is the purpose of the recommended extra storage tubes? The extra stor...

    Preparation of Bottom-Gate OFET using P3HT

    Plexcore OS 1100, a p-type polymer semiconductor, is a moderate molecular weight grade of P3HT that is optimized for Organic Field Effect Transistor (OFET) applications. It has been used successfully in top and bottom-gate OFET architectures with a variety of device dimensions, substrates, and diele...

    Water for Next-Generation Sequencing

    Water is used throughout the next-generation sequencing (NGS) workflow, from DNA fragmentation to sequencing runs and instrument cleaning. Using the appropriate water quality for each step will not only eliminate the risk of water contaminants interfering with nucleic acids and the many enzymatic re...

    An Alternative to the USP and EP Methods for the Analysis of Riboflavin (Vitamin B2)

    Related Articles • Reporter US Volume 33.3 (4 Mb PDF) • UHPLC Analysis of Taxanes Using A Titan C18 Column and UV Detection • LC-MS Analysis of Vitamin D Metabolites • HPLC Separation of Nine Key Silymarin Components of Milk Thistle Using a Titan™ C18 Column with LC/MS/MS Detection An alternative to...

    Sera-Mag™ SpeedBeads Magnetic Protein A/G-Particles Protocol

    Introduction Important information before using Sera-Mag SpeedBeads Protein A/G Magnetic Particles Procedure for manual antibody purification Procedure for automated antibody purification Procedure for manual immunoprecipitation (IP) Procedure for automated immunoprecipitation Troubleshooting Materi...

    Carbon Nanomaterials: Elemental Analyses and Quantification of Their Accumulation in Living Cells

    Introduction Elemental Analyses of CNMs Benefits of an Extensive Elemental Analysis of CNMs Quantifying CNMs Accumulated by Biological Cells Gel Electrophoresis Method to Quantify CNMs Gel Electrophoresis Method to Quantify CNMs in Aqueous Samples Gel Electrophoresis Method to Quantify CNMs Extracte...

    Handling and Storage of Synthetic Peptides

    One of the most challenging aspects of working with synthetic peptides is determining the best solvent in which to dissolve the peptide. General guidelines for proper storage, handling and dissolving your synthetic peptide are provided below. Lyophilized peptides are shipped at ambient temperature a...

    CelLytic B Plus

    For efficient protein extraction of Gram+ and Gram- Bacteria The CelLytic B Plus Kit is designed to efficiently lyse cells and extract proteins (Figure 1) from both Gram negative, and difficult to lyse, Gram positive bacteria. This is accomplished using the standard CelLytic B, a proprietary non-ion...

    Selected Applications of Metal-Organic Frameworks in Sustainable Energy Technologies

    • • Download PDF • Stephen R. Caskey Adam J. Matzger Stephen R. Caskey1 and Adam J. Matzger2 1Sigma-Aldrich Corp., Milwaukee, WI 53209 2Department of Chemistry and the Macromolecular Science and Engineering Program, University of Michigan, Ann Arbor, MI 48109 Email: stephen.caskey@sial.com, matzger@...

    Carbon Nanofibers- Processing and Dispersing

    Carbon nanofibers (CNFs) are discontinuous, highly graphitic, highly compatible with most polymer processing techniques, and can be dispersed in an isotropic or anisotropic mode. They have excellent mechanical properties, high electrical conductivity, and high thermal conductivity, which can be impa...

    Graphene Nanoribbons: Production and Applications

    Ayrat M. Dimiev1 and James M. Tour2 1EMD Performance Materials Corp., 70 Meister Ave, Somerville, NJ 08876 USA 2Departments of Chemistry, Materials Science and NanoEngineering, and Computer Science, and the Smalley Institute for Nanoscale Science and Technology Rice University, MS-222, 6100 Main Str...