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Search term:"sonication"

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115 matches found for sonication

CelLytic B

This is a highly efficient (Figure 1 a and Figure 1 b ) yet gentle reagent for the extraction of proteins from bacteria (E. coli). This reagent is a proprietary formulation of zwitterionic detergents in 40 mM Trizma®-HCl (pH 8.0). Treatment of bacterial cells with CelLytic B results in rapid and eff...

BugBuster® Plus Lysonase™ Kit: Cell lysis and nucleic acid removal for Gram-negative and Gram-positive bacteria

BugBuster® Protein Extraction Reagent has become an indispensable tool for efficient extraction of active soluble proteins from E. coli without the need for sonication or any other method of mechanical disruption. Lysonase™ Bioprocessing Reagent is a blend of rLysozyme™, a highly purified and stabil...

Evaluation of the reproducibility of Parallel Artificial Membrane Permeation Assays (PAMPA)

Daniel Schmidt and John Lynch, PhD EMD Millipore Corporation, Life Science Division, Danvers, MA The reproducibility and precision of an automation compatible, high throughput method for predicting passive, intestinal absorption was assessed. The Parallel Artificial Membrane Permeation Assay (PAMPA)...

Handling Inclusion Bodies

Related Articles • Affinity Chromatography in a Purification Strategy (CIPP) • Characteristics of Dextrin Sepharose® High Performance Products • Characteristics of Glutathione Sepharose ® Products • Characteristics of Ni Sepharose®, Ni Sepharose® excel, TALON® Superflow™, and Uncharged IMAC Sepharose®...

CoMoCAT® Single-wall Carbon Nanotubes

Related Products • Carbon Nanomaterials • Graphene Technologies • Nanomaterials Resources Related Articles • Carbon Nanomaterials: Elemental Analyses and Quantification of Their Accumulation in Living Cells • Boron Nitride Nanotubes: Properties, Synthesis and Applications • Novel Properties and Appl...

Normal-Phase Methodology in Solid Phase Extraction

Basic Steps Common Normal-Phase Solvents Non-polar Sample Matrix/ Mobile Phase Environment In order for polar retention to occur between the sorbent and the sample, the analyte must be introduced to the SPE device in a non-polar sample or mobile phase environment. Therefore, typical sample matrices ...

Western Blotting Sample Preparation

Chapter 2, Extracted from Western Blotting Principles and Methods, GE Healthcare Life Science, 2011 Symbols This symbol indicates general advice on how to improve procedures or recommends measures to take in specific situations ** indicates a third party trademark The importance of good sample prepa...

Recycling Micro-Assay of β-NAD and β-NADH

• • Download PDF • 1. OBJECTIVE To standardize a procedure for the recycling micro-assay of β-NAD and β-NADH at Sigma-Aldrich Saint Louis. 2. SCOPE This procedure applies to products that have a specification for the recycling micro-assay of β-NAD and β-NADH. 3. DEFINITIONS 3.1. Purified Water = wat...

Sera-Mag™ and Sera-Mag SpeedBeads Magnetic Particles

Introduction Sera-Mag Magnetic SpeedBeads Sera-Mag Magnetic Particles Materials Sera-Mag and Sera-Mag SpeedBeads provide cost effective magnetic bead separation technology for molecular biology applications, nucleic acid isolation, and research immunoassays. Sera-Mag superparamagnetic particles comb...

sonic hedgehog
Synonyms HHG1, HLP3, HPE3, MCOPCB5, SMMCI, TPT, TPTPS
Species Human (6469) , Species Mouse (20423) , Species Rat (29499) , Species chicken (395615) , Species domestic cat (100169727) , Species dog (608860) , Species cow (286821) , Species sheep (780492) , Species sheep (101117666) , Species domestic guinea pig (100732014) , Species naked mole-rat (101719896) , Species Domestic Rabbit (100352774)
Summary: This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities. Additionally, mutations in a long range enhancer located approximately 1 megabase upstream of this gene disrupt limb patterning and can result in preaxial polydactyly. [provided by RefSeq, Jul 2008]

BugBuster Protein Extraction Reagent in new configurations for convenience and versatility in soluble protein extraction

BugBuster® Protein Extraction Reagent is formulated for the gentle disruption of the cell wall of E. coli, liberating soluble protein. It provides a simple, rapid, low-cost alternative to mechanical methods such as French Press or sonication for releasing expressed target protein in preparation for ...

Biochemicals Coupling to Microparticles

Besides Proteins, DNA, lectins and many other species have been coupled to microparticles. Coupling to microparticles may be achieved by passive adsorption or covalent attachment. Most techniques using passive adsorption technology report four to six months of bead stability. This technology is the ...

Sampling Ammonia using radiello Passive Sampler

The following article was generated with the assistance of an outside source using Sigma-Aldrich® products. Content was provided from the public information published on the Ammonia Monitoring Network website. http://nadp.isws.illinois.edu Introduction The National Atmospheric Deposition Program (NA...

Chromatin Immunoprecipitation (ChIP)

One of the most powerful tools available for investigating epigenetic mechanisms of disease is chromatin immunoprecipitation (ChIP). ChIP can detect and relatively quantify specific protein-DNA and protein- protein interactions in vivo at a single or multiple loci. ChIP involves chemically crosslink...

Molecular Monolayers on Silicon Surfaces

• • Download PDF • Steacie Institute for Molecular Sciences, 100 Sussex Dr., Ottawa, Ontario, Canada E-mail: Gregory.Lopinski@nrc-cnrc.gc.ca Gregory P. Lopinski* and Daniel D.M. Wayner Controlled formation of organic molecular monolayers on silicon surfaces offers the promise of enhancing the functi...

Synthesis of Halide Perovskite Quantum Dots for Display Applications

School of Materials Science & Engineering, Beijing Institute of Technology, Beijing, 100081, China *Email: hzzhong@bit.edu.cn Colloidal quantum dots (QDs) are solution-processable luminescent materials that can produce high-quality color in light emitting diodes (LEDs). These LEDs are essential comp...

Water for Next-Generation Sequencing

Water is used throughout the next-generation sequencing (NGS) workflow, from DNA fragmentation to sequencing runs and instrument cleaning. Using the appropriate water quality for each step will not only eliminate the risk of water contaminants interfering with nucleic acids and the many enzymatic re...

Functionalization of Fluorescent Nanodiamonds for Bioimaging

Related Products • Fluorescent Nanodiamonds • PEGs & PEOs • Nanomaterials Related Content • Nanomaterial Bioconjugation Techniques • Monodispersed Nanodiamonds & their Applications • Nanomaterials What are Fluorescent Nanodiamonds? Fluorescent nanodiamonds (FNDs) exhibit distinct properties such as ...

Purification of Histidine-Tagged Proteins using IMAC Sepharose 6 Fast Flow

Related Articles • Affinity Chromatography in a Purification Strategy (CIPP) • Characteristics of Dextrin Sepharose® High Performance Products • Characteristics of Glutathione Sepharose ® Products • Characteristics of Ni Sepharose®, Ni Sepharose® excel, TALON® Superflow™, and Uncharged IMAC Sepharose®...

Preparative Purification Using GSTPrep FF 16/10 Column

GSTPrep FF 16/10 columns are based on the 20 ml HiPrep column design, ready to use for easy, one-step preparative purification of GST-tagged proteins, other glutathione S-transferases, and glutathione binding proteins. Prepacked with Glutathione Sepharose 4 Fast Flow, the columns exhibit high bindin...

Simple Purification Using a GSTrap Column with ÄKTAprime™

ÄKTAprime, in combination with pre-installed method templates for purifications and prepacked columns, is designed to perform the most common protein purification steps with the touch of one button. It provides significant advantages in speed, capacity, and fraction selection compared with manual pu...

Simple, efficient extraction of protein from hepatocytes with CytoBusterTM Reagent

Cytochromes p450 (CYP) are a large group of heme-containing mono-oxygenases which catalyse many important biological functions (1). These most notably include the oxidative conversions of many steroids, lipids, environmental toxins and xenobiotics. This family of enzymes plays a significant role in ...

Frequently Asked Questions About Chromatin Immunoprecipitation (ChIP)

For more information about ChIP experiment guides, troubleshooting tips and supplementary protocols, please view our Chromatin Immunoprecipitation (ChIP) page. Antibodies 1. Should I use a monoclonal or polyclonal antibody? Either monoclonal or polyclonal antibodies can work for ChIP. Monoclonals ar...

Bacterial Cell Lysis

CelLytic products are a family of protein extraction reagents specifically formulated to lyse and extract cellular proteins based on the type of expression system. CelLytic proprietary formulations for bacterial cell lysis are available in a range of products specific to your protein extraction need...

BugBuster® and Benzonase® Reagents are the Clear Solutions to Simple, Efficient Extraction of E. coli Proteins

There are a variety of methods for cellular disintegration and extraction of proteins from E. coli ranging from enzymatic digestion and osmotic shock to ultrasonication and pressure disruption. Each method has inherent advantages and disadvantages. Generally, vigorous mechanical treatments reduce vi...

Sample Preparation & Gel Electrophoresis: Western Blot Troubleshooting

Related Links • Western Blotting (Immunoblotting) Protocol • 30-minute Immunodetection Protocol Using the SNAP i.d.® 2.0 System • Sample Preparation Methods from GE Healthcare • Products for Cell Lysis and Protein Extraction • Western Blotting Home Page • Western Blotting Literature & Resources • We...

Separation of Ginsenosides in Panax Quinquefolius (American Ginseng)

Related Information • Analytix 3, 2015 (PDF) Belonging to the plant genus panax, ginseng is one of the most well-known herbal medicines. It has been used in traditional Chinese medicine for centuries, but has also become very popular in the Western world. Medicines derived from the ginseng root are ...

Precise Nanoparticles for Optoelectronics Applications

Benny Pacheco* and Scott Kordyban** Product and Business Development Manager Cytodiagnostics, Inc. 919 Fraser Dr, Unit 11 Burlington, ON L7L 4X8 *Email: bpacheco@cytodiagnostics.com **Not pictured Inorganic nanomaterials are tunable by size, shape, structure, and/or composition. Advances in the synt...

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

Related Articles • Affinity Chromatography in a Purification Strategy (CIPP) • Characteristics of Dextrin Sepharose® High Performance Products • Characteristics of Glutathione Sepharose ® Products • Characteristics of Ni Sepharose®, Ni Sepharose® excel, TALON® Superflow™, and Uncharged IMAC Sepharose®...

Array CGH Analysis of Challenging Samples

In recent years, array-based Comparative Genomic Hybridization (aCGH) has been refined to determine chromosomal changes at progressively higher resolutions1. This evolving technology is, however, hampered by the large DNA input requirement—a minimum of 150,000 copies of a human genome, or 0.5 μg, ar...

ChIP Supplementary Protocols

1. If necessary, stimulate or treat adherent mammalian cells at ~80 to 90% confluence in a 150 mm culture dish containing 20 mL of growth media. Include one extra plate of cells to be used solely for estimation of cell number. 2. Prepare 22 mL of 1X PBS (2.2 mL 10X PBS and 19.8 mL water) for each 15...