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    Search term:"sonication"

    103 matches found for sonication

    Purification of Histidine-Tagged Recombinant Proteins Using Ni Sepharose High Performance

    Extracted from Affinity Chromatography Vol. 2: Tagged Proteins, GE Healthcare, 2016 Ni Sepharose High Performance consists of highly cross-linked 6% agarose beads (34 µm) to which a chelating group has been immobilized and subsequently charged with Ni2+ ions. The chromatography medium provides very ...

    Purification using StrepTactin Sepharose High Performance

    Related Articles • Affinity Chromatography in a Purification Strategy (CIPP) • Characteristics of Dextrin Sepharose® High Performance Products • Characteristics of Glutathione Sepharose ® Products • Characteristics of Ni Sepharose®, Ni Sepharose® excel, TALON® Superflow™, and Uncharged IMAC Sepharose®...

    Sampling Ammonia using radiello Passive Sampler

    The following article was generated with the assistance of an outside source using Sigma-Aldrich® products. Content was provided from the public information published on the Ammonia Monitoring Network website. http://nadp.isws.illinois.edu Introduction The National Atmospheric Deposition Program (NA...

    Precise Nanoparticles for Optoelectronics Applications

    Benny Pacheco* and Scott Kordyban** Product and Business Development Manager Cytodiagnostics, Inc. 919 Fraser Dr, Unit 11 Burlington, ON L7L 4X8 *Email: bpacheco@cytodiagnostics.com **Not pictured Inorganic nanomaterials are tunable by size, shape, structure, and/or composition. Advances in the synt...

    Peptide Quick Tips

    Common impurities found in custom peptide material can include any of the following: • Sequences with amino acid deletions • Incomplete peptide sequences (truncation) • Sequences containing incompletely deprotected amino acids • Sequences modified as a result of the cleavage process (i.e. reattachme...

    Purification using StrepTrap HP 1 ml and 5 ml

    Extracted from Affinity Chromatography Vol. 2: Tagged Proteins, GE Healthcare, 2016 StrepTrap™ HP 1 ml and 5 ml columns are made of biocompatible polypropylene that does not interact with biomolecules (Figure 7.2). Prepacked StrepTrap™ HP columns provide fast, simple, and easy separations in a conve...

    Troubleshooting Purification Methods

    Related Articles • Affinity Chromatography in a Purification Strategy (CIPP) • Characteristics of Dextrin Sepharose® High Performance Products • Characteristics of Glutathione Sepharose ® Products • Characteristics of Ni Sepharose®, Ni Sepharose® excel, TALON® Superflow™, and Uncharged IMAC Sepharose®...

    Liposome Preparation - Avanti® Polar Lipids

    Liposomes (lipid vesicles) are formed when thin lipid films or lipid cakes are hydrated and stacks of liquid crystalline bilayers become fluid and swell. The hydrated lipid sheets detach during agitation and self-close to form large, multilamellar vesicles (LMV) which prevents interaction of water w...

    Purification using Dextrin Sepharose High Performance

    Related Articles • Affinity Chromatography in a Purification Strategy (CIPP) • Characteristics of Dextrin Sepharose® High Performance Products • Characteristics of Glutathione Sepharose ® Products • Characteristics of Ni Sepharose®, Ni Sepharose® excel, TALON® Superflow™, and Uncharged IMAC Sepharose®...

    sonic hedgehog
    Synonyms HHG1, HLP3, HPE3, MCOPCB5, SMMCI, TPT, TPTPS
    Species Human (6469) , Species Mouse (20423) , Species Rat (29499) , Species chicken (395615) , Species domestic cat (100169727) , Species dog (608860) , Species cow (286821) , Species sheep (780492) , Species sheep (101117666) , Species domestic guinea pig (100732014) , Species naked mole-rat (101719896) , Species Domestic Rabbit (100352774)
    Summary: This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities. Additionally, mutations in a long range enhancer located approximately 1 megabase upstream of this gene disrupt limb patterning and can result in preaxial polydactyly. [provided by RefSeq, Jul 2008]

    Graphene and Other 2D Crystals for Rechargeable Batteries

    Francesco Bonaccorso,* Vittorio Pellegrini Istituto Italiano di Tecnologia, Graphene Labs, 16163, Genova, Italy *Email: francesco.bonaccorso@iit.it Advanced technologies for energy conversion and storage are widely sought after for their potential to improve consumer and electronic device performanc...

    Handling and Storage of Synthetic Peptides

    One of the most challenging aspects of working with synthetic peptides is determining the best solvent in which to dissolve the peptide. General guidelines for proper storage, handling and dissolving your synthetic peptide are provided below. Lyophilized peptides are shipped at ambient temperature a...

    Probes for 19F Magnetic Resonance Imaging: PERFECTA

    PERFECTA is not available in the US. 19F Magnetic Resonance Imaging (19F MRI) is gaining momentum as a powerful diagnostic modality among currently available noninvasive bioimaging techniques.1 The absence of endogenous organic 19F signals in living tissues allows for in vivo visualization of exogen...

    Troubleshooting Strategies in Purification of GST-tagged Proteins

    This troubleshooting guide addresses the common problems associated with the majority of purification methods using the different Glutathione Sepharose media. Problem Possible cause Solution GST-tagged protein does not bind or binds poorly to the medium. The flow rate used during sample loading is t...

    Procedure for Preparation of Cationic Lipid/Oligomer Complex - Avanti® Polar Lipids

    Normal procedure for preparation of lipid:DNA complex requires suspending the cationic lipid in aqueous buffer and sonication to form small vesicles. The cationic lipid vesicles are mixed with the aqueous DNA solution at a weight ratio of 10:1, lipid:DNA, and incubated for a period of time. During t...

    Purification of Histidine-Tagged Proteins using TALON Superflow

    Extracted from Affinity Chromatography Vol. 2: Tagged Proteins, GE Healthcare, 2016 TALON® Superflow consists of highly cross-linked agarose beads with an immobilized chelating group precharged with Co2+ ions. The medium binds polyhistidine-tagged proteins with high selectivity and exhibits a reduced...

    Single-Walled Carbon Nanotubes: Exfoliation and Debundling Procedure

    The dispersibility and bundle defoliation of single-walled carbon nanotubes (SWCNTs), which can be applied to materials produced by the CoMoCAT® process, have been extensively investigated by SouthWest NanoTechnologies (SWeNT ®) and at the University of Oklahoma.1 The procedure employed to disperse ...

    Simple Purification Using a GSTrap Column with ÄKTAprime™

    ÄKTAprime, in combination with pre-installed method templates for purifications and prepacked columns, is designed to perform the most common protein purification steps with the touch of one button. It provides significant advantages in speed, capacity, and fraction selection compared with manual pu...

    Chromatin Immunoprecipitation Kit CHP1 Protocol

    Product Description Storage/Stability Workflow Preparation Instructions Procedure Analysis Troubleshooting Guide Frequently Asked Questions Materials References Poster & Application Note Protein-DNA interactions play critical roles in cellular functions including signal transduction, gene transcript...

    Troubleshooting Methods for Purification of MBP-Tagged Recombinant Proteins

    Related Articles • Affinity Chromatography in a Purification Strategy (CIPP) • Characteristics of Dextrin Sepharose® High Performance Products • Characteristics of Glutathione Sepharose ® Products • Characteristics of Ni Sepharose®, Ni Sepharose® excel, TALON® Superflow™, and Uncharged IMAC Sepharose®...

    Purification using Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B

    Extracted from Affinity Chromatography Vol. 2: Tagged Proteins, GE Healthcare, 2016 These three chromatography media are all used for the purification of GST-tagged recombinant proteins and other S-transferases or glutathione-dependent proteins. They allow mild elution conditions that preserve protei...

    Preparative Purification Using GSTPrep FF 16/10 Column

    GSTPrep FF 16/10 columns are based on the 20 ml HiPrep column design, ready to use for easy, one-step preparative purification of GST-tagged proteins, other glutathione S-transferases, and glutathione binding proteins. Prepacked with Glutathione Sepharose 4 Fast Flow, the columns exhibit high bindin...

    Screening pGEX Recombinants for Tagged Protein Expression

    Sections of this procedure have been adapted with permission from Current Protocols in Molecular Biology, Vol. 2, Supplement 10, Unit 16.7. Copyright © 1990 by Current Protocols. The following steps may be used prior to large-scale purification to check clones for expression of the desired tagged pr...

    Performing a Cell Lysis of Recombinant Histidine-Tagged Proteins

    Related Articles • Affinity Chromatography in a Purification Strategy (CIPP) • Characteristics of Dextrin Sepharose® High Performance Products • Characteristics of Glutathione Sepharose ® Products • Characteristics of Ni Sepharose®, Ni Sepharose® excel, TALON® Superflow™, and Uncharged IMAC Sepharose®...

    Bacterial Cell Lysis

    CelLytic products are a family of protein extraction reagents specifically formulated to lyse and extract cellular proteins based on the type of expression system. CelLytic proprietary formulations for bacterial cell lysis are available in a range of products specific to your protein extraction need...

    Cleavage and Purification of GST-Tagged Protein Bound to GSTrap

    Recommended buffers Binding buffer: PBS: 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.3 For PreScission Protease cleavage: Elution buffer: Cleavage buffer: PreScission Protease 50 mM Tris-HCl, 10 mM reduced glutathione, pH 8.0 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1 mM DTT, pH 7.0 F...

    Protease Inhibitor Cocktails References

    The Sigma-Aldrich portfolio of protease inhibitor cocktails shows an excellent track record in the peer-reviewed scientific literature, as witnessed by the many publications that cite our protease inhibitor cocktails. This page lists a sampling of those research papers. Other citations are available...

    April 2001 Lysis of E. Coli

    A TECHNICAL APPLICATION NEWSLETTER VOL 2 ISSUE 2 April 2001 Molecular Biology Application Notes Lysis of E. coli for the Purification of Soluble Recombinant Proteins using CelLytic-BTM and CelLytic-BTMII By Rick Mehigh, Sigma-Aldrich Corporation, St. Louis, MO USA Introduction The first step in the ...

    Sera-Mag™ SpeedBeads Magnetic Protein A/G-Particles Protocol

    Introduction Important information before using Sera-Mag SpeedBeads Protein A/G Magnetic Particles Procedure for manual antibody purification Procedure for automated antibody purification Procedure for manual immunoprecipitation (IP) Procedure for automated immunoprecipitation Troubleshooting Materi...

    Performing a purification and on-column refolding of an insoluble histidine-tagged protein from a 100 ml E. coli culture using HisTrap FF 1 ml with ÄKTAprime plus

    Extracted from Purifying Challenging Proteins Principles and Methods, GE Healthcare, 2007 This procedure uses a HisTrap FF 1 ml column but can also be used with a HisTrap HP 1 ml or a HisTrap FF crude 1 ml column. The procedure uses ÄKTAprime plus but can also be run on other ÄKTA systems. Buffers R...

    An Alternative to the USP and EP Methods for the Analysis of Riboflavin (Vitamin B2)

    Related Articles • Reporter US Volume 33.3 (4 Mb PDF) • UHPLC Analysis of Taxanes Using A Titan C18 Column and UV Detection • LC-MS Analysis of Vitamin D Metabolites • HPLC Separation of Nine Key Silymarin Components of Milk Thistle Using a Titan™ C18 Column with LC/MS/MS Detection An alternative to...