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121 matches found for thin_layer_chromatography

Trypsin Serine Protease Enzyme

Related Links • Trypsin Inhibitors • Cell Dissociation with Trypsin Trypsin is a member of the serine protease S1 family. It consists of a single chain polypeptide of 223 amino acid residues. The native form of trypsin is refered to as β-trypsin. Autolysis of β-trypsin (which is cleaved at Lys131- S...

BioUltra Reagents Detergents & Surfactants

Introduction Structures of common surfactants Analytical Information Applications References Alphabetical List of Products Introduction Surfactants constitute the most important group of detergent components. Generally, these are water-soluble surface-active agents comprised of a hydrophobic portion...

HPTLC Application for Passiflora incarnata

In previous articles we presented HPTLC (High- Performance Thin-Layer Chromatography) applications for a series of medicinal plants, such as Ginkgo (Analytix 5/2016), Hypericum (Analytix 1/2017) or Ginseng (Analytix Reporter Issue 2/2018). With this article, we continue this series with an applicati...

Fermentation: Enzymes, Substrates and SCFA

The influence of probiotics on the metabolic activity of intestinal microbiota may be evaulated by detecting bacterial enzymatic activity in fecal samples, specifically β-glucosidase, β-glucuronidase, and urease activity. Experimental studies using probiotics have indicated a significant decrease in...

New Synthetic Lipids

Lipids and their metabolism play a crucial role in the physiology of cells, tissues and organs, and the system-level analysis of lipids and their interactions can be seen as a sub-discipline of metabolomics. Sigma-Aldrich® supports the rapid advances in metabolic pathway research and lipid applicati...

One Solvent (DMAc), two bases (NaOtBu, Cs2CO3) Step-by-Step Guide for Buchwald-Hartwig Amination Reaction Screening Kit

2 bases (NaOt-Bu, 2 M in THF and Cs2CO3) 1 solvent (DMAc) 12 pre-weighed catalysts Materials required - set up: • 1 mylar bag from the KitAlysis Buchwald-Hartwig Amination Reaction Screening Kit and you will use the following components - 12 x 2 pre-weighed catalysts in glass vials loaded with stir ...

Stille Kit

Product No. 802336 Compound Catalog No. Equivalents MW Density Amount mmol Kit Components Pd[P(t-Bu)3]2 676578 0.03 511.05 7.7 mg 0.015 DABCO D27802 3.0 112.17 170 mg 1.50 NaCl S9888 1.0 58.44 30 mg 0.50 TPGS-750-M 733857 1.002 2.0 mL User Supplied Aryl bromide [ArBr] 1.0 0.50 Organotributyltin reag...

Live Cell Calcium Indicators

Calcium ions (Ca2+) play vital cellular physiology roles in signal transduction pathways, in neurotransmitter release, in contraction of all muscle cell types, as enzyme cofactors, and in fertilization. Extracellular calcium is also important for maintaining the potential difference across excitable...

Fmoc Resin Cleavage and Deprotection

Having successfully synthesized a protected peptide, one is confronted with a difficult task of having to simultaneously detach the peptide from the resin support and remove all the side-chain protecting groups of the amino acid residues to yield the desired peptide. In Fmoc SPPS, this step is norma...

Purification Consumables for Analytical & Process Scale Chromatography

We understand that your purification challenges are unique. That’s why we offer you a variety of options to help you solve them. Whether you seek the up-front cost savings and versatility of packing your own columns, the consistent quality and time savings of pre-packed columns, or the ease associat...

Separation of Steviol Glycosides by HPTLC: Introducing a New Stevia Extract Reference Material

Download the flyer Analytix Reporter Issue 6 | 2019 Related Products • View Product List • Stevia Standards • Phytochemical Reference Materials • Sweetener Standards Related Articles • Profiling of Stevia rebaudiana Extract by Accurate Mass Using HILIC and Reversed-Phase Chromatography (Reporter 27....

Probiotics and Human Health

At the beginning of the 20th century, probiotics were thought to have a beneficial effect to the host by improving the intestinal microbial balance. Through his studies and observations, the Russian biologist Metchnikoff noted people from cultures which consumed large amounts of fermented milk, gene...

Two solvents (Dioxane, DMAc), strong base (NaOt-Bu) Step-by-Step Guide for Buchwald-Hartwig Amination Reaction Screening Kit

1 base (NaOt-Bu, 2 M in THF) 2 solvents (1,4 Dioxane and DMAc) 12 pre-weighed catalysts Materials required - set up: • 1 mylar bag from the KitAlysis Buchwald-Hartwig Amination Reaction Screening Kit and you will use the following components - 12 x 2 pre-weighed catalysts in glass vials loaded with ...

Analytical Enzyme Chymotrypsin

E.C. 3.4.21.1 On this page: • Products • Physical Properties • Specificity and Kinetics • Inhibitors • Substrates • Solubility and Solution Stability Molecular Weight: 25 kDa1 (Bovine) pI: 8.752 (Bovine) Extinction coefficient: E1%= 20.4 (280nm) Chymotrypsin is produced in the acinar cells of the pa...

Step-by-Step Guide for KitAlysis™ High-Throughput Photocatalysis Reaction Screening Kit

24 pre-weighed catalysts 4 degassed solvents (DCM, DCE, DMF, MeCN) Note: While the above scheme depicts a generic decarboxylative coupling reaction, High-Throughput Photocatalysis Screening Kit can be used for any photocatalytic transformation Materials Required - Set up: • 1 mylar bag from the KitA...

Food and Beverage

To the scientists tasked with keeping us all safe from food born illnesses, we are committed to advancing food and beverage safety and quality today, by developing the most trusted and reliable products and services providing improved lab testing efficiency regulatory compliance for food manufacture...

Purer Fmocs Means Purer Peptides

All peptide chemists recognize the key to obtaining high purity peptides is to maximize efficiency of every synthetic step. However, not everyone realizes that obtaining high purity peptides also depends on using starting materials of the highest quality - small improvements in starting material qua...

BioUltra Reagents

Introduction Amino Acids Antioxidants Biological Buffers Carbohydrates Chelators Crystallization Denaturation Detergents/Surfactant Density Gradient Molecular Biology Luminescence Spectroscopy Reagents The BioUltra label has been assigned to a group of basic reagents with a well-defined high purity....

Polymer-Bound Lewis Acids

Lewis acids are important catalysts for many types of transformations in organic chemistry. Lanthanide salts serve as mild alternatives to traditional hard Lewis acids. Reactions catalyzed by the polymerbound lanthanide Lewis acids include acetalization, aldol reaction and allylation of aldehydes, n...

Two solvents (Dioxane, DMAc), weak base (Cs2CO3) Step-by-Step Guide for Buchwald-Hartwig Amination Screening Kit

1 base (Cs2CO3, ground and sieved) 2 solvents (1,4 Dioxane and DMAc) 12 pre-weighed catalysts Materials required - set up: • 1 mylar bag from the KitAlysis Buchwald- Hartwig Amination Reaction Screening Kit and you will use the following components - 12 x 2 pre-weighed catalysts in glass vials loade...

Suzuki Kit

Product No. 802271 Compound Catalog No. Equivalents MW Density Amount mmol Kit Components Pd(dtbpf)Cl2 701602 0.026 651.75 9 mg 0.013 Triethylamine T0886 3.3 101.19 0.726 0.23 mL 1.65 TPGS-750-M 733857 1.002 1.0 mL User Supplied Aryl bromide [ArBr] 1.0 0.5 Arylboronic acid [ArB(OH)2] 1.0-2.0 0.5-1.0...

Enzyme Explorer Key Resources: Coagulation Proteins & Reagents

Anticoagulant Reagents Factor V Factor VIII Factor IX Factor X Factor XII Fibrin/Fibrinogen Kallikrein Kininogen Plasmin/Plasminogen Platelet Activators Protein C Thrombin/Prothrombin (Factor II) Plasma & Blood Protein Resource Anticoagulant Reagents Product Product # Heparin sodium salt bovine inte...

Sample Preparation & Purification in Analytical Chemistry & Chromatography

Our sample preparation and purification portfolio includes products for solid phase extraction (SPE), solid phase microextraction (SPME), air monitoring, sample decomposition, purge and trap techniques and flash chromatography and LPLC. Our comprehensive range features products for clinical, pharmac...

Polyethyleneimine Based

Number AL-142 Ion-Exchange Resins and Related Polymeric Adsorbents Home | Amberlite® and Amberlyst® Resins | Dowex® Resins | Sephadex® Products Merrifield Peptide Resins | Polyethylenimine-Based Products | Polyamide Resins V. POLY(ETHYLENIMINE)-SUPPORTED ION-EXCHANGE RESINS AND RELATED PRODUCTS Work...

Bile Acids Kit for LC-MS

A Rapid and Reliable Kit for Bile Acids Research Bile Acids (BA) are synthesized in the liver and play important roles in cholesterol homeostasis, absorption of vitamins and lipids, and various key metabolic processes. BA analysis is an important area of study in various settings including clinical ...

Glassware Catalog

Air-Sensitive Ampules Beakers Boiling Media Bottles and Caps Burettes Culture Tubes Glass TLC Plates: Silica matrix Diazomethane Generators Evaporators and Accessories Extractors Flasks Gas Tubes and Traps Bubblers Chromatography Columns Condensers Crucibles Desiccators Dewars Dishes Distillation Ap...

High-Performance Thin-Layer Chromatography: A Fast and Efficient Fingerprint Analysis Method for Medicinal Plants

Ginsenosides are triterpene saponins. Most ginsenosides are composed of a dammarane skeleton (17 carbons in a four-ring structure) with various sugar moieties (e.g. glucose, rhamnose, xylose and arabinose) attached to the C- 3 and C-20 positions. Over 30 ginsenosides have been identified and classif...

Small Molecules Drug Development

When analyzing small molecules the overarching need is to maximize sensitivity without compromising reproducibility and laboratory throughput. We have developed unique tools to maximize speed and selectivity throughout the entire workflow. Our tools support the requisite techniques, such as gas chro...

Medium (5,6,7) Ring-Closing Metathesis Procedure

6 pre-weighed catalysts (x4) 4 degassed solvents: Chlorobenzene (ClPh), Toluene (PhMe), Isopropyl Acetate (i-PrOAc), and Methyl-THF (Me-THF) 1 (optional) acid additive (degassed neat TFA) • 1 mylar (foil) bag from the KitAlysis Grubbs Metathesis Reaction Screening Kit and you will use the following ...

Analytical Chemistry Key Competencies and Technologies

Review our core competencies, innovative technologies, areas of specialization, and product lines for the Analytical marketplace. To start the discussion about your specific request, business proposal, or idea for collaboration, please contact us. E-mail: analytical@sial.com • Overview of Key Compet...