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308 matches found for transcription

Kava (Piper methysticum) | Plant Profiler

Synonyms / Common Names / Related Terms (+) -dihydrokawain-5-ol, 11-methoxy-5, 5-hydroxydihydrokawain, 6-dihydroyangonin, antares, ava, ava pepper, ava pepper shrub, ava root, awa, bornyl cinnamate, cavain, Fijian kava, flavokavines A and B, gea, gi, grog, intoxicating long pepper, intoxicating pepp...

Induced Pluripotent Stem Cell Culture Protocols

Related Products • EBiSC iPS Cell Lines • Pluripotent Stem Cell Culture • Stem Cell Culture • Stem Cell Protocols • MEF Feeder Cells • 3D Cell Culture • Primary Cell Culture • ECACC Cancer Cell Lines • Cell Culture Media and Reagents • Stericup® Media Filtration • Growth Factors • ECM Proteins • Ant...

Diverse CD4 Response to Vaccinia Virus in Human is Revealed by Proteome-Wide T cell Profiling

• • Download PDF • Authors: Lichen Jing,1 D. Huw Davies,2 Tiana M. Chong,3 Sookhee Chun,2 Christopher L. McClurkan,3 Jay Huang,3 Brian T. Story,3 Douglas M. Molina,4 Siddiqua Hirst,2 Philip L. Felgner,2 and David M. Koelle1,2,5,6,7*, Department of Medicine, University of Washington, Seattle, Washing...

Acetylcholine Synthesis and Metabolism

Acetylcholine is synthesized from acetyl coenzyme A and choline by the enzyme choline acetyltransferase. In the nervous system, this enzyme is thought to exist primarily in the nerve terminal cytoplasm. Coenzyme A is synthesized in mitochondria and accesses choline acetyltransferase following transp...

Ephedra (Ephedra sinica) | Plant Profiler

Synonyms / Common Names / Related Terms 6-hydroxykynurenic acid, amp II, amsania, brigham tea, budshur, cao ma huang (Chinese), cathine, chewa, Chinese ephedra, Chinese joint fir, D-pseudoephedrine, desert herb, desert tea, dextro-rotatory, EPH 833, Ephedra altissima, Ephedra americana, Ephedra anti...

EGF/EGFR signaling pathway

Background Epidermal Growth Factor Receptors (EGFR) and ligands Receptor activation EGFR Signaling Ras/Raf signaling cascade Phosphatidylinositol 3-kinase/Akt signaling cascade Signal transducers and activators of transcription (STAT) pathway Phospholipase Cγ signaling Nck/PAK signaling cascade Cbl ...

RNAi Question and Answer Archive from the TRC

Experts in the field of RNAi have answered your questions. You will find your answers here. I would like to know whether anyone has used shRNA on primary human monocytes and if so is there any advice? I want to lyse plant cells at various stages and analyze the miRNA (siRNA) population. What vector ...

Methods of Quantification

Standard Curves Standard curves are necessary for both absolute and relative quantification. When generating standard curves, different concentrations of DNA (typically five) should be used to generate a standard curve that will bracket the concentration of the unknown. Each concentration should be ...

Plasmid DNA purification kits by Sigma Aldrich

Extraction of macromolecules such as DNA, RNA, and protein is one of the basic methods used in molecular biology. The process of extraction and purification of nucleic acids has evolved from being a complex, prolonged, and labor-intensive procedure. Nucleic acid purification technologies now give hi...

transcription factor EC
Synonyms TCFEC, TFE-C, TFEC-L, TFECL, bHLHe34, hTFEC-L
Species Human (22797) , Species Mouse (21426) , Species Rat (26296) , Species domestic guinea pig (100717764) , Species naked mole-rat (101697964) , Species cow (539016) , Species sheep (101109437) , Species Zebrafish (556894) , Species chicken (417773) , Species dog (475292) , Species domestic cat (101085109)
Summary: This gene encodes a member of the micropthalmia (MiT) family of basic helix-loop-helix leucine zipper transcription factors. MiT transcription factors regulate the expression of target genes by binding to E-box recognition sequences as homo- or heterodimers, and play roles in multiple cellular processes including survival, growth and differentiation. The encoded protein is a transcriptional activator of the nonmuscle myosin II heavy chain-A gene, and may also co-regulate target genes in osteoclasts as a heterodimer with micropthalmia-associated transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]

MISSION™ shRNA Library: Next Generation RNAi

By Stephanie Uder, Henry George, and Betsy Boedeker Sigma-Aldrich Corporation, St. Louis, MO, USA Introduction The technology of RNA interference emerged in its earliest form following a 1998 study in Caenorhabditis elegans1and has since rapidly evolved to its current form as a revolutionary tool fo...

Duolink® PLA Applications

With Duolink® PLA, you can detect, quantify, and visualize protein-protein interactions, post-translational modifications, and low expression protein detection with an easy-to-execute immunodetection protocol that is similar to traditional IF or IHC experiments, but yields far more sensitive signals...

ThermaStop™-RT PCR Additive

Related Products • Transcriptor One-Step RT-PCR Kit • Transcriptor Reverse Transcriptase • JumpStart™ Taq ReadyMix™ for Quantitative PCR Related Articles • ThermaGo™ • ThermaStop™ • ThermaGenix Product Overview Related Content • PCR/Amplification • Hot Start PCR • PCR Selection Guide Improved RT-PCR...

MAPKs

The mitogen-activated protein kinase (MAPK) family consists of both stress activated (SAPK) and mitogen-activated (MAPK) protein kinases. They form a network of signal transduction cascades that mediate cellular responses to a diverse range of stimuli, including growth factors, chemical or osmotic s...

Plant RNA/DNA Purification Kit

Norgen’s Plant RNA/DNA Purification Kit provides a rapid method for the isolation and purification of total RNA and DNA simultaneously from a single sample of plants. The total RNA and DNA (including genomic DNA) and are both column purified in under 30 minutes using a single column. It is often nec...

Transplex® RNA Amplification Kit: Whole Transcriptome Amplification of Highly-Degraded RNA from FFPE Tissues.

Ken Heuermann and Brian Ward Research and Development, Biotechnology Division, Sigma-Aldrich The TransPlex Complete Whole Transcriptome Amplification (WTA2 ) Kit effectively amplifies intact and highly degraded RNA. To benchmark maintenance of representative RNA levels during amplification, differen...

GenomePlex® Amplification of ChIP DNA

GenomePlex® Amplification of ChIP DNA Testimonial References Protocols Related Products ChIP-chip is a powerful tool that combines chromatin immunoprecipitation (ChIP) with microarrays to study protein-DNA interactions genome-wide. The ChIP step utilizes an antibody directed against a target protein...

esiRNA Libraries Targeting Long Non-Coding RNA (lncRNA)

On this page: • Introduction to Non-Coding RNA • Product offering • Request a Quote or more information • In situ hybridization data • esiRNA specifications • How to Order • esiRNA controls • Reference Introduction to Non-Coding RNA Eukaryotic genomes contain both protein-coding and non-coding regio...

Naive Pluripotent Stem Cell Culture in an Inhibitor Based 2i/LIF Containing Serum-Free and Feeder-Free Stem Cell Media

Related Products • Stem Cell Culture • Stem Cell Protocols • 3D Cell Culture • Primary Cell Culture • ECACC Cancer Cell Lines • Cell Culture Media and Reagents • Growth Factors • ECM Protein • Antibodies • Bioactive Small Molecules • CRISPR Genome Editing Introduction Methods Results Conclusions Mat...

Sanger Whole Genome CRISPR Arrayed Libraries

Designed by the Wellcome Trust Sanger Institute Free On-Demand Webinar: Let's Talk Lentivirus Sanger Arrayed Lentiviral CRISPR Libraries won prestigious R&D 100 Award We have joined forces with The Wellcome Sanger Institute to make the first ever arrayed lentiviral CRISPR knockout libraries for huma...

Assessing Enriched Murine CD4+ T Cells Differentiated Towards Effector T Helper Cell Lineages

Cellular differentiation, the process by which progenitor cells differentiate into specialized cell types, is fundamental to developmental biology. In the immune system, immune progenitor cells must differentiate into diverse innate and adaptive phenotypes for the body to respond to infections. In t...

Cell Line Engineering and the Cell Design Studio Webinar

Webinar Title: Cell Line Engineering and the Cell Design Studio Date: Tuesday, March 27, 2018 Time: 11:00am EST Duration: 60 minutes Gene editing has become a firmly established technology within the discovery sciences arena. With the advent of CRISPR/Cas9 systems, the researcher's ability to find a...

Building Blocks for Introducing Post-translational Modified Amino Acids

The Novabiochem® product line has always been at the forefront of the development for methods for the synthesis of peptides containing PTMs, being the first company to provide effective building blocks for synthesis of phosphorylated peptides, and sulfotyrosine and methylated arginine containing pep...

Sample Purification & Quality Assessment - PCR Technologies Guide

• Introduction and Historical Timelines • Polymerase Chain Reaction • Quantitative PCR • Digital PCR • Quantitative PCR and Digital PCR Detection Methods • PCR qPCR dPCR Assay Design • Sample Purification and Quality Assessment • Reverse Transcription • Assay Optimization and Validation • Data Analy...

Antifungals

Antifungal compounds have been overshadowed by antibacterials in research interest and application due to the greater impact bacterial infections have had on health. Resistance to antibacterial drugs and the resultant clinical impact is of widespread concern regarding public health. However, resista...

DIG RNA Labeling Mix Protocol & Troubleshooting

Product No. 11277073910 Evaluation of DIG RNA labeling efficiency: Determine the labeling efficiency in terms of μg (expected yield of a standard labeling reaction is 20 μg of DIG labeled RNA per μg linearized template DNA after the DIG RNA labeling reaction). Roche recommends loading an aliquot on ...

Purification or Removal of DNA-Binding Proteins

Extracted from Affinity Chromatography Vol. 3: Specific Groups of Biomolecules, GE Healthcare, 2014 DNA-binding proteins form an extremely diverse class of proteins sharing a single characteristic, their ability to bind to DNA. Functionally the group can be divided into those responsible for the repli...

RNA Stabilization at Room Temperature in RNAstable®

Introduction RNAstable is a novel preservation product developed to protect RNA from degradation during storage or shipment at ambient temperatures. The synthetic storage medium is based on the natural principles of anhydrobiosis (meaning “life without water”), a biological mechanism employed by som...

Genome engineering using the CRISPR-Cas9 system

Nature Protocols Volume 8 Issue 11, 24 November 2013, Pages 2281 - 2308 doi: 10.1038/nprot.2013.143 Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. F Ann Ran a, b, c, d, e , Patrick D Hsu a, b, c, d, e , Jason Wright a , Vineeta Agarwala a, f, g , David A Sc...

Growth Factors in Stem Cell Biology

Related Links • Growth factors • 3D-cell culture • Organoids • Pluripotent Stem Cell Culture • Stem cell basics Related Products • Cell Culture Media and Reagents • Growth Factors • Stem Cell Antibodies • CRISPR Genome Editing • 3D Cell Culture • ECACC cell lines • 3DKUBE • 3D Hydrogels Growth facto...

RNAstable®/ RNAstable® LD Handbook

• Product Formats • Introduction • Principles and Procedures • Material needed by user • Storage • Applications • Important Notes • Protocol: Sample preparation • Protocol: Sample drying and storage • Protocol: Sample recovery • Protocol: Downstream applications • Troubleshooting Guide • Appendix A:...