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267 matches found for transduction

RNAi Screening Webinar

• Abstract • Speaker RNAi screening has made it possible to identify new genes, or gene networks, that are involved in a wide vari­ety of biological processes, including assays relevant to signal transduction, cell viability, cell morphology, protein localization and function, drug resistance, and r...

MISSION® ExpressMag™ System FAQ

What is the coating on the ExpressMag beads? The ExpressMag beads are coated with a propriety polymeric shell that enables it to bind to the surface of most viral particles. Will the ExpressMag system only enhance the transduction of MISSION lentiviral particles? We have explicitly validated Express...

Vascular Endothelial Growth Factor (VEGF)

Background VEGF Pathway Ligands Receptors VEGF – Signal Transduction Factors Regulating VEGF and VEGFR Expression VEGFR in Human Diseases Conclusion Formation of new blood vessels, a process known as angiogenesis, is a fundamental biological process required for embryonic development, tissue repair,...

Lentivirus Protocols

Successful Transduction Using Lentivirus Protocol provided by the MISSION® R&D Team Safe Handling of Lentivirus Protocol provided by the MISSION® R&D Team CRISPR Lentiviral Screening: Antibiotic Selection Protocol provided by the MISSION® R&D Team Protocol, FACS Titration of Lentivirus Protocol prov...

Frequently asked Questions on Choosing an RNAi Format

I am new to RNAi work. Which should I choose, siRNA or shRNA? Both methods provide viable knockdown efficiency, to better help you choose please refer to the table below. Choosing the Appropriate RNAi Effector for your Application siRNA shRNA, Transfected plasmid shRNA, Lentivirus-Delivered Cell Lin...

Library Content

What does it mean if I cannot find my target gene in the database? The collection is currently being cloned. New target genes will be introduced on a quarterly basis until the library is completed. It is possible your target gene is not in the current collection, but may be coming in future releases...

Lentiviral Systems

If I want to make my own viral particles, which format should I purchase? What should I do if I have trouble obtaining high yields of plasmid DNA? How many cells can I infect with the amount of lentivirus provided? How does Sigma measure viral titer? What is the method you recommend for screening th...

Lentiviral Magnetic Transduction [VIDEO]

In this exercise, the hard to transduce human melanoma cell line, CRL-1676 will be transduced with MISSION™ TurboGFP Control Transduction particles coupled with ExpressMag magnetic beads as a solution to delivering DNA to hard-to-transduce cells. CRL-1676 cells will also be transduced with a standar...

Biology and Analytical Chemistry Webinar Guide

Biology Webinar Guide Quality Systems and Regulatory Compliance Drug Discovery Webinar Guide Analytical Webinar Guide Materials Science Webinar Guide • Proteomics • qPCR • CRISPR • Cell Culture • Cell Design Studio • Duolink • RNAi • Regulatory Compliance • Late Stage Functionalization of Drug-Like ...

KH domain containing, RNA binding, signal transduction associated 1
Synonyms Sam68, p62, p68
Species Human (10657) , Species Mouse (20218) , Species Rat (117268) , Species chicken (374071) , Species domestic cat (101080467) , Species dog (487316) , Species cow (538775) , Species sheep (101117825) , Species domestic guinea pig (100725478) , Species naked mole-rat (101708086)
Summary: This gene encodes a member of the K homology domain-containing, RNA-binding, signal transduction-associated protein family. The encoded protein appears to have many functions and may be involved in a variety of cellular processes, including alternative splicing, cell cycle regulation, RNA 3'-end formation, tumorigenesis, and regulation of human immunodeficiency virus gene expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012]

Publications: Online library of tutorials, product guides and references

• Life Science and Biochemicals • Chromatography & Analytical • Materials Science, Chemical Synthesis and Lab Equipment • Newsletter Subscription • Molecular Biology Handbook • Cloning Manual • Synthetic Biology Resources • qPCR Technical Guide Life Science and Biochemicals Name Description Antibody...

Live Cell Calcium Indicators

Calcium ions (Ca2+) play vital cellular physiology roles in signal transduction pathways, in neurotransmitter release, in contraction of all muscle cell types, as enzyme cofactors, and in fertilization. Extracellular calcium is also important for maintaining the potential difference across excitable...

Fak

The focal adhesion kinase (FAK) is a cytoplasmic protein tyrosine kinase that distinctly co-localizes with integrins at sites of attachment to their ligands. In cells in culture, these sites are manifested as regions of close contact with the underlying substrate called focal adhesions. Attachment o...

Fluorescence Lifetime Measurement Advantages & Applications

Fluorescence lifetime (FLT) is the time a fluorophore spends in the excited state before emitting a photon and returning to the ground state. FLT can vary from picoseconds to hundreds of nanoseconds depending on the fluorophore. The lifetime of a population of fluorophores is the time measured for t...

Transient Receptor Potential Channels

The transient receptor potential family of ion channels consists of at least 28 mammalian members divided into six subfamilies: 7 TRPC (canonical), 6 TRPV (vanilloid), 8 TRPM (melastatin), 1 TRPA (ankyrin), 3 TRPP (polycystin) and 2 TRPML (mucolipin). Numerous invertebrate TRP channels have also bee...

Successful Transduction Using Lentivirus

Successful targeting relies on optimizing key sensitive steps in the process, including lentiviral transduction. Below are some helpful handling and titration tips from our R&D lentiviral experts. As always, contact our technical support if you have any questions about your genome modification proje...

Universal ProbeLibrary System User Statements and Application

The Universal ProbeLibrary - A Versatile Tool for Quantitative Expression Analysis in the Zebrafish (PDF) The comparison of the Universal ProbeLibrary-based qPCR to a conventional SYBR Green-I-based qPCR assay using embryonic zebrafish cDNA. Real-Time PCR Quantification of Plant miRNAs Using Univers...

FGFR

Fibroblast growth factors (FGFs) comprise a large family of signaling polypeptides. In the adult organism, FGFs are homeostatic factors and function in tissue repair and response to injury. In the embryo, FGFs regulate cell proliferation, migration, differentiation and survival. When inappropriately...

Sea buckthorn (Hippophae rhamnoides) | Plant Profiler

Synonyms / Common Names / Related Terms 5-formyltetrahydrofolate, 5-hydroxytryptamine, 5-methyltetrahydrofolate, acetic acid, aekol, alkaloids, almindelig havtorn (Danish), alpha-linolenic acid, amino acid, Amritscherl (German), anthocyanin, arabinose, argasse, argasse (French), argouse (French), ar...

shRNA Frequently Asked Questions for Experimental Design

What controls do you recommend? Why doesn't the non-target control vector affect gene expression? How many cells can I infect with the amount of lentivirus provided? What is the method you recommend for screening the stable clones and is there a pair of primers that can be used for screening them by...

Lentiviral Production Using X-tremeGENE HP Transfection Reagent

Lentiviruses represent a powerful tool in research applications to transduce a wide range of cell types. During virus production, low titers and toxicity can be challenging. This easy-to-use protocol uses X-tremeGENE HP Transfection Reagent for fast and robust lentiviral preparation, with high virus...

Determining Relative Transduction of Adherent Cells Using MISSION® TurboGFP™ Transduction Particles

Product No. SHC003V Protocol provided by MISSION® Team Go directly to: • Introduction • Materials • Protocol • Tips • Figure Introduction MISSION® TurboGFP™ control particles enable fast determination of the appropriate transduction conditions for efficient transduction of cells with lentiviral part...

Ordering & Timelines - MISSION shRNA FAQ

Can I order MISSION shRNA clones over the phone? Currently the best way to order is over the web. If you have a special situation or need assistance with your order please email us at MISSIONRNAi@sial.com. Why don't the MISSION shRNA have product numbers? MISSION shRNA products are made on demand an...

Unique Search Tools for Cell Signaling & Neuroscience

Antibody Explorer – Find the primary antibodies, secondary antibodies and sera you need for specific applications Browse for Antibodies • Gene ID Search • Primary Antibodies • Secondary Antibodies • Kits • Supplementary Products • Antibody Alphabetical Index • Clone Index Technical Resources • Antib...

MISSION® shRNA Application Data

Sigma-Aldrich® is committed to providing you with helpful application notes and data for the MISSION® TRC shRNA libraries. Below are links to data pieces and articles on various topics of interest. If you would like us to post your data (specific applications, validation of gene targets, etc.) on ou...

Lentiviral Transduction [VIDEO]

In this exercise we will be demonstrating lentiviral transduction on two different cell lines. In the first example the human neuroblastoma cell line SK-N-SH will be transduced with MISSION TurboGFP Control particles. A range of MOI’s will be tested and an increase in GFP positive cells will be obse...

Biology and Analytical Chemistry Video Guides

Biology Video Guide Analytical Video Guide • Biochemistry • Cell Culture • Genomics • RNAi • ZFNs • General HPLC (High Performance Liquid Chromatography) • Ascentis Columns • Protein Gel Electrophoresis • Miscellaneous Biology Video Guide Title Topics Covered Length Biochemistry ATP Synthase Animati...

Choosing a Format - shRNA Frequently Asked Questions

In what formats can the shRNA clones be ordered? Target sets consisting of 3-5 clones are available for 10,858 human and 8,424 mouse genes. They may be ordered as complete target sets or individual clones. 3 formats are available for each target set/clone: bacterial glycerol stock, purified plasmid ...

Garlic (Allium sativum) | Plant Profiler

Synonyms / Common Names / Related Terms 2-propenesulfenic acid, aged garlic extract, aglio, ail, ail commun, ajo, ajoene, akashneem, alisat, allicin, Allii sativi bulbus, alliinase, allium, allyl mercaptan, alubosa elewe, Amaryllidaceae (family), ayo-ishi, ayu, banlasun, camphor of the poor, clove g...

Quantitative Image Analysis and Emerging Applications on the ImageStream Imaging Flow Cytometer Webinar

Webinar Title: Quantitative Image Analysis and Emerging Applications on the ImageStream Imaging Flow Cytometer The ImageStream® and FlowSight® are multispectral imaging flow cytometers that generates high resolution images of cells at a rate of 1000’s of cells per second. This allows for the rapid a...

Sweet Taste Perception

• • Download PDF • The powerful effect of the pleasurable sensation arising from sweet tasting foods has led to consumer demand and large-scale production of the caloric sweetening agents sucrose and fructose. Due to economic considerations and sugar shortages, consumer demand for alternative sweete...