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81 matches found for trizma base 1503

U1131 - Enzyme Assay (23 KB)

SSPLAS01 Page 1 of 3 Revised: 01/22/96 SIGMA QUALITY CONTROL TEST PROCEDURE Enzymatic Assay of UROKINASE (EC 3.4.21.73) PRINCIPLE: Plasminogen + H2O Urokinase> Plasmin Casein Plasmin> Perchloric Acid Soluble Amino Acids CONDITIONS: T = 37°C, pH = 7.5, A275nm, Light path = 1 cm METHOD: Spectrophotome...

A7550 - Enzyme Assay (22 KB)

A7550 Page 1 of 3 SPMICR01 Revised: 06/25/97 SIGMA QUALITY CONTROL TEST PROCEDURE Enzymatic Assay of ACHROMOPEPTIDASE (EC 3.4.21.50) PRINCIPLE: Micrococcus lysodeikticus Cells (intact) Achromopeptidase > Micrococcus lysodeikticus Cells (lysed) CONDITIONS: T = 37EC, pH 8.0, A600nm, Light path = 1 cm ...

Glycogen Synthase Kinase 3B (17 KB )

Revised: 08/26/99 Page 1 of 4 Enzymatic Assay of GLYCOGEN SYNTHASE KINASE 3B PRINCIPLE: PI-2 + γ-32P-ATP GSK > [ 32P]-Phosphorylated PI-2 + ADP Abbreviations used: PI-2 = Phosphatase Inhibitor-2 γ-32P-ATP = Adenosine 5-Triphosphate γ 32-P label GSK = Glycogen Synthase Kinase 3B ADP = Adenosine 5'-Tr...

Penicillinase Benzylpenicillin as Substrate (15 KB )

Revised: 01/11/95 Page 1 of 3 Enzymatic Assay of PENICILLINASE (EC 3.5.2.6) Benzylpenicillin as Substrate PRINCIPLE: Benzylpenicillin + H 2O Penicillinase> Benzylpenicilloic Acid CONDITIONS: T = 25°C, pH = 7.0 METHOD: Titrimetric REAGENTS: A. 100 mM Tris HCl Buffer with 0.1% (w/v) Bovine Serum Album...

Thrombocytin (13 KB)

Revised: 01/21/94 Page 1 of 2 Enzymatic Assay of THROMBOCYTIN PRINCIPLE: CONDITIONS: T = 37°C, pH 8.4, A405nm, Light path = 1 cm METHOD: Continuous Spectrophotometric Rate Determination REAGENTS: A. 100 mM Tris and 100 mM Imidazole Buffer with 150 mM Sodium Chloride, pH 8.4 at 37°C (Prepare 100 ml i...

C2905 - Enzyme Assay (25 KB)

SSCHON01 Page 1 of 4 Revised: 04/24/97 SIGMA QUALITY CONTROL TEST PROCEDURE Enzymatic Assay of CHONDROITINASE ABC (EC 4.2.2.4) Sigma Prod. No. C-2905 PRINCIPLE: Chondroitin Sulfate + H 2O Chondroitinase ABC> Unsaturated Disaccharides CONDITIONS: T = 37°C, pH = 8.0, A232nm, Light path = 1 cm METHOD: ...

Chondroitinase B (16 KB )

Revised: 05/06/94 Page 1 of 3 Enzymatic Assay of CHONDROITINASE B PRINCIPLE: Chondroitin Sulfate B + H2O Chondroitinase B> Unsaturated Uronic Acid CONDITIONS: T = 25°C, pH 7.5, A232nm, Light path = 1 cm METHOD: Spectrophotometric Stop Rate Determination REAGENTS: A. 20 mM Tris HCl Buffer with 50 mM ...

Urokinase (19 KB)

SSPLAS01 Page 1 of 3 Revised: 01/22/96 Enzymatic Assay of UROKINASE (EC 3.4.21.73) PRINCIPLE: Plasminogen + H2O Urokinase> Plasmin Casein Plasmin> Perchloric Acid Soluble Amino Acids CONDITIONS: T = 37°C, pH = 7.5, A275nm, Light path = 1 cm METHOD: Spectrophotometric Stop Rate Determination REAGENTS...

A6302 - Enzyme Assay (14 KB)

Revised: 01/17/97 Page 1 of 4 SIGMA QUALITY CONTROL TEST PROCEDURE Enzymatic Assay of AMINOACYL-tRNA SYNTHETASE (EC 6.1.1) PRINCIPLE: tRNA + 14 C Arginine + ATP > tRNA - 14 C Arginine + AMP + Pyrophosphate CONDITIONS: T = 37°C, pH = 7.6 METHOD: Radiolabelled Stop Reaction REAGENTS: A. 1 M Tris HCl B...

G3P Oxidase Spectrophotometric Stop Rate (16 KB )

SSGLYC01 Page 1 of 4 Revised: 08/07/98 Enzymatic Assay of GLYCEROL-3-PHOSPHATE OXIDASE 1 (EC 1.1.3.21) PRINCIPLE: DL-a-Glycerophosphate + O 2 GPO > Dihydroxyacetone phosphate + H 2O2 2H2O2 + 4-AAP + Phenol POD > Quinoneimine Dye + 4H2O Abbreviations used: GPO = Glycerol-3-Phosphate Oxidase 4-AAP = 4...

Lysostaphin (18 KB )

06/22/95 Page 1 of 3 Enzymatic Assay of LYSOSTAPHIN PRINCIPLE: Staphylococcal Cells (intact) Lysostaphin> Staphylococcal Cells (lysed) CONDITIONS: T = 37°C, pH 7.5, A620nm, Light path = 1 cm METHOD: Turbidimetric Rate Determination REAGENTS: A. 50 mM Tris HCl Buffer with 145 mM Sodium Chloride, pH 7...

Aminoacyl-trna Synthetase (22 KB)

Revised: 01/17/97 Page 1 of 4 Enzymatic Assay of AMINOACYL-tRNA SYNTHETASE (EC 6.1.1) PRINCIPLE: tRNA + 14C Arginine + ATP > tRNA - 14C Arginine + AMP + Pyrophosphate CONDITIONS: T = 37°C, pH = 7.6 METHOD: Radiolabelled Stop Reaction REAGENTS: A. 1 M Tris HCl Buffer with 50 mM Magnesium Chloride, 50...

L4402 - Enzyme Assay (13 KB)

L4402 Page 1 of 3 SSTRYP04 06/22/95 SIGMA QUALITY CONTROL TEST PROCEDURE Enzymatic Assay of LYSOSTAPHIN PRINCIPLE: Lysostaphin Staphylococcal Cells (intact) > Staphylococcal Cells (lysed) CONDITIONS: T = 37ffC, pH 7.5, A620nm, Light path = 1 cm METHOD: Turbidimetric Rate Determination REAGENTS: A. 5...

A5212 - Enzyme Assay (29 KB)

A5212 Page 1 of 4 SPACET05 Revised: 08/30/96 SIGMA QUALITY CONTROL TEST PROCEDURE Enzymatic Assay of ALDEHYDE DEHYDROGENASE (EC 1.2.1.5) PRINCIPLE: Acetaldehyde + β-NAD Aldehyde Dehydrogenase > Acetic Acid + β-NADH Abbreviations used: β-NAD = β-Nicotinamide Adenine Dinucleotide, Oxidized Form β-NADH...

Lipase Triacetin (16 KB)

VETRIA01 Page 1 of 3 Revised: 04/21/97 Enzymatic Assay of LIPASE (EC 3.1.1.3) (Triacetin as Substrate) PRINCIPLE: Triacetin + H2O Lipase > Glycerol + Acetic Acid CONDITIONS: T = 37°C, pH = 7.4 METHOD: Titrimetric REAGENTS: A. 150 mM Tris HCl Buffer with 330 mM Triacetin, pH 7.4 at 37°C (Buffered Tri...

Succinyl Coenzyme A Transferase (18 KB)

Revised: 10/05/94 Page 1 of 3 Enzymatic Assay of SUCCINYL COENZYME A TRANSFERASE (EC 2.8.3.5) PRINCIPLE: Acetoacetyl CoA + Succinate SCT > Succinyl CoA + Acetoacetate Abbreviations used: SCT = Succinyl Coenzyme A Transferase Acetoacetyl CoA = Acetoacetyl Coenzyme A Succinyl CoA = Succinyl Coenzyme A...

Chondroitinase Ac (16 KB)

Revised: 01/21/94 Page 1 of 3 Enzymatic Assay of CHONDROITINASE AC (EC 4.2.2.5) PRINCIPLE: Chondroitin Sulfate + H2O Chondroitinase AC> Unsaturated Disaccharides CONDITIONS: T = 37°C, pH = 7.3, A232nm, Light path = 1 cm METHOD: Continuous Spectrophotometric Rate Determination REAGENTS: A. 250 mM Tri...

Aldehyde Dehydrogenase (21 KB)

SPACET05 Page 1 of 3 Revised: 08/30/96 Enzymatic Assay of ALDEHYDE DEHYDROGENASE (EC 1.2.1.5) PRINCIPLE: Acetaldehyde + ß-NAD Aldehyde Dehydrogenase> Acetic Acid + ß-NADH Abbreviations used: ß-NAD = ß-Nicotinamide Adenine Dinucleotide, Oxidized Form ß-NADH = ß-Nicotinamide Adenine Dinucleotide, Redu...

L7386 - Enzyme Assay (13 KB)

L7386 Page 1 of 3 SSTRYP04 06/22/95 SIGMA QUALITY CONTROL TEST PROCEDURE Enzymatic Assay of LYSOSTAPHIN PRINCIPLE: Lysostaphin Staphylococcal Cells (intact) > Staphylococcal Cells (lysed) CONDITIONS: T = 37ffC, pH 7.5, A620nm, Light path = 1 cm METHOD: Turbidimetric Rate Determination REAGENTS: A. 5...

Enzymatic Assay of PROTEASE INHIBITOR (23 KB)

Revised: 01/06/96 Page 1 of 4 Enzymatic Assay of PROTEASE INHIBITOR, of Calcium Activated Neutral Protease PRINCIPLE: N,N-Dimethylated Casein + H2O Protease, Calcium Activated Neutral> Amino Acids This reaction is inhibited by the protease inhibitor. CONDITIONS: T = 30°C, pH = 7.5, A293nm, Light pat...

I4402 - Enzyme Assay (26 KB)

L4402 Page 1 of 3 SSTRYP04 06/22/95 SIGMA QUALITY CONTROL TEST PROCEDURE Enzymatic Assay of LYSOSTAPHIN PRINCIPLE: Lysostaphin Staphylococcal Cells (intact) > Staphylococcal Cells (lysed) CONDITIONS: T = 37EC, pH 7.5, A620nm, Light path = 1 cm METHOD: Turbidimetric Rate Determination REAGENTS: A. 50...

Adenosine 5'-Triphosphatase pH 7 (22 KB )

SS-ATP03 Page 1 of 5 Revised: 05/02/96 Enzymatic Assay of ADENOSINE 5'-TRIPHOSPHATASE (EC 3.6.1.3) PRINCIPLE: ATP + H 2O ATPase > ADP + Pi Abbreviations used: ATPase = Adenosine 5'-Triphosphatase ATP = Adenosine 5'-Triphosphate ADP = Adenosine 5'-Diphosphate Pi = Inorganic Phosphate CONDITIONS: T = ...

Phospholipase A2 Colorimetric (17 KB)

Revised: 03/23/94 Page 1 of 4 Enzymatic Assay of PHOSPHOLIPASE A2 (EC 3.1.1.4) PRINCIPLE: Phospholipase A2 L-a-Lecithin + H2O > L-a-Lysolecithin + fatty acids Abbreviations Used: L-a-Lecithin = L-a-Phosphatidylcholine L-a-Lysolecithin = L-a-Lysophosphatidylcholine CONDITIONS: T = 37°C, pH 8.5, A570n...

A3422 - Enzyme Assay (22 KB)

A3422 Page 1 of 3 SPMICR01 Revised: 06/25/97 SIGMA QUALITY CONTROL TEST PROCEDURE Enzymatic Assay of ACHROMOPEPTIDASE (EC 3.4.21.50) PRINCIPLE: Micrococcus lysodeikticus Cells (intact) Achromopeptidase > Micrococcus lysodeikticus Cells (lysed) CONDITIONS: T = 37EC, pH 8.0, A600nm, Light path = 1 cm ...

A3518 - Enzyme Assay (14 KB)

Revised: 01/17/97 Page 1 of 4 SIGMA QUALITY CONTROL TEST PROCEDURE Enzymatic Assay of AMINOACYL-tRNA SYNTHETASE (EC 6.1.1) PRINCIPLE: tRNA + 14 C Arginine + ATP > tRNA - 14 C Arginine + AMP + Pyrophosphate CONDITIONS: T = 37°C, pH = 7.6 METHOD: Radiolabelled Stop Reaction REAGENTS: A. 1 M Tris HCl B...

C5896 - Enzyme Assay (20 KB)

Revised: 03/17/94 Page 1 of 4 SIGMA QUALITY CONTROL TEST PROCEDURE Enzymatic Assay of CHOLINE OXIDASE (EC 1.1.3.17) PRINCIPLE: Choline + O2 Choline Oxidase > Betaine Aldehyde + H2O2 Betaine Aldehyde + O2 + H2O Choline Oxidase > Betaine + H2O2 2H2O2 + 4-Aminoantipyrine + Phenol POD > Quinoneimine dye...

Sphingomyelinase Colorimetric (18 KB )

SSTNPA01 Page 1 of 4 Revised: 12/04/95 Enzymatic Assay of SPHINGOMYELINASE 1 from Bacterial Sources (EC 3.1.4.12) PRINCIPLE: TNPAL-Sphingomyelin + H 2O Sphingomyelinase> Choline Phosphate + TNPAL-N-Acylsphingosine Abbreviations: TNPAL-Sphingomyelin = Trinitrophenylaminolauroyl-Sphingomyelin TNPAL-N-...

Heparinase I (15 KB )

Revised: 02/01/95 Page 1 of 3 Enzymatic Assay of HEPARINASE I (EC 4.2.2.7) PRINCIPLE: Heparin + H2O Heparinase I> Unsaturated Uronic Acid CONDITIONS: T = 25°C, pH = 7.5, A235nm, Light path = 1 cm METHOD: Spectrophotometric Stop Rate Determination REAGENTS: A. 20 mM Tris HCl Buffer with 50 mM Sodium ...

A3547 - Enzyme Assay (22 KB)

A3547 Page 1 of 3 SPMICR01 Revised: 06/25/97 SIGMA QUALITY CONTROL TEST PROCEDURE Enzymatic Assay of ACHROMOPEPTIDASE (EC 3.4.21.50) PRINCIPLE: Micrococcus lysodeikticus Cells (intact) Achromopeptidase > Micrococcus lysodeikticus Cells (lysed) CONDITIONS: T = 37EC, pH 8.0, A600nm, Light path = 1 cm ...

G1663 - Enzyme Assay (14 KB)

Revised: 08/26/99 Page 1 of 4 SIGMA QUALITY CONTROL TEST PROCEDURE Enzymatic Assay of GLYCOGEN SYNTHASE KINASE 3B PRINCIPLE: PI-2 + γ-32 P-ATP GSK > [ 32 P]-Phosphorylated PI-2 + ADP Abbreviations used: PI-2 = Phosphatase Inhibitor-2 γ-32 P-ATP = Adenosine 5-Triphosphate γ 32 -P label GSK = Glycogen...