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USP HPLC Analysis of Carbamazepine on an Ascentis® Express ES-CN Column

column Ascentis Express ES-Cyano, 10 cm x 4.6 mm I.D., 2.7 µm particles (53491-U) column temp. 30 °C mobile phase (A)water, (B)methanol, (C)THF; (850:120:30, A:B:C) and 0.22 mL formic acid, 0.5 ml triethylamine per liter flow rate 1.25 mL/min pressure 3480 psi (240 bar) sample 0.2 mg/mL in water:met...

USP HPLC Analysis of L-Methionine on Ascentis® Express OH5

column Ascentis Express OH5 15 cm x 4.6 mm, 5 µm particles (50347-U) column temp. 40 °C mobile phase [A] 0.005M Ammounium formate in Water (pH: 3.15 with Trifluoroacetic Acid) ), [B] Acetonitrile (30:70, A:B) flow rate 1.0 mL/min pressure 914 psi (63 bar) sample 0.8 mg/mL water:acetonitrile (1:1) in...

USP Dissolution Testing Method (HPLC) for Folic Acid Tablets using a Monolith Column and UV detection

Related Products • Analytical Standards • Analytical Reagents • Technical Compendium • Applications Search • HPLC Folic acid or folate is classified as a B vitamin (B9). Folic acid is synthetically produced, and used in fortified foods and supplements. Folate is converted by humans to dihydrofolate ...

USP Method for the HPLC Analysis of 4-Amino-6-Chloro-1,3-Benzenedisulfonamide on Ascentis® Express C18

column Ascentis Express C18, 5 cm x 4.6 mm I.D., 2.7 µm particles (53826-U) column temp. 30 °C mobile phase [A] formic acid in water (5:1000); [B] acetonitrile:methanol (3:1) gradient 3% B for 5 min; to 36% B in 14 min; to 3% B in 18 min flow rate 1.0 mL/min pressure 1189 psi (82 bar) sample 0.32 mg...

USP HPLC Analysis of Ampicillin Sodium on Ascentis® Express C18

column Ascentis Express C18, 10 cm x 4.6 mm I.D., 5 mum particles (50536-U) column temp. 30 °C mobile phase [A:B:C:D] [A] acetonitrile, [B]water, [C] 1M Potassium phosphate monobasic in water, [D] 1N acetic acid in water (80:909:10:1) flow rate 1.0 mL/min sample 1 mg/mL in mobile phase injection 10 ...

USP Method for Fexofenadine and Pseudoephedrine Identification Test B Using TLC Plates

Related Links • Analytical Standards and Certified Reference Materials • Analytical Reagents and Solvents • Generic Pharmaceutical & Release Testing • Applications Search • Thin Layer Chromatography Fexofenadine is a selective peripheral H1-blocker, an antihistamine drug, used in the treatment of al...

USP Analysis of Actos (Pioglitazone) using Ascentis® Express C18

column Ascentis® Express C18, 15 cm x 4.6 mm, 5 µm particles (50537-U) column temp. 25 °C mobile phase [A] 100 mM ammonium acetate; [B] acetonitrile; [C] acetic acid; (55:45:2, A:B:C) flow rate 0.7 mL/min pressure 1160 psi (80 bar) sample pioglitazone 50 µg/mL, benzophenone 13 µg/mL in mobile phase ...

USP Dissolution Testing Method (HPLC) for Esomeprazole Magnesium Delayed Release Capsules

Esomeprazole is the S-enantiomer of omeprazole. Esomeprazole is a proton pump inhibitor and reduces acid secretion through inhibition of the H+ / K+ ATPase in gastric parietal cells. By inhibiting the functioning of this transporter, the drug prevents formation of gastric acid. It is used in the tre...

Miconazole USP Method for organic Impurities Using HPTLC Plates

Miconazole, sold under the brand name Monistat among others, is an antifungal medication used to treat ring worm, pityriasis versicolor, and yeast infections. Current US pharmacopeia (USP41-NF36) indicates that a TLC plate with an adsorbent having 0.25 mm layer of chromatographic silica gel mixture ...

Alfuzosin USP Monograph – HPLC method for Related Substances

Alfuzosin is an antagonist of the α1 adrenergic receptor and it relaxes the muscles in the prostate and bladder neck, making it easier to urinate. It is used to treat benign prostatic hyperplasia (BPH), and it was approved by the United States FDA in June 2003. Alfuzosin is branded and marketed by S...

HPLC Analysis of Simvastatine USP on Chromolith® Performance SpeedRod 50-4.6mm

sample preparation 1.4mg/ml monobasic potassium phosphate in water column Chromolith® Performance SpeedRod 50-4.6mm (1.51450.0001) column temp. ambient mobile phase [A] 50% Acetonitril, 50% phosphoric acid and water 1:999; [B] 100% phosphoric acid and Acetonitril 1:999; gradient Initial composition:...

HPLC Analysis of Simvastatine USP on Chromolith® Performance Flash 25-4.6mm

sample preparation 1.4mg/ml monobasic potassium phosphate in water column Chromolith Performance Flash 25-4.6mm (1.51463.0001) column temp. ambient mobile phase [A] 50% Acetonitril, 50% phosphoric acid and water 1:999; [B] 100% phosphoric acid and Acetonitril 1:999; gradient Initial composition:0-4....

Sildenafil Citrate USP Method Using HPTLC Plates for the Limit of Imidazole Test

Sildenafil, sold under the brand name Viagra, among others, is a medication that can be used as a selective inhibitor of cyclic GMP- specific photodiesterase (type V) with vasodilator action. It can be used to treat erectile dysfunction and pulmonary arterial hypertension. In 1994, Pfizer filed a pa...

HPLC Analysis of Simvastatine USP on Chromolith High Resolution RP-18e (analyte set 2)

sample preparation 1.4mg/ml monobasic potassium phosphate in water - Diluent: Acetonitril and Buffer 3:2 column Chromolith High Resolution RP-18 endcapped, 50 x 4.6 mm (1.52021.0001) column temp. ambient mobile phase [A] 50% Acetonitril, 50% phosphoric acid and water 1:999; [B] 100% phosphoric acid ...

HPLC Analysis of Simvastatine USP on Chromolith High Resolution RP-18e (analyte set 1)

sample preparation 1.4mg/ml monobasic potassium phosphate in water. Diluent: Acetonitril and Buffer 3:2 column Chromolith High Resolution RP-18 endcapped, 100 x 4.6 mm (1.52022.0001) column temp. ambient mobile phase [A] 50% acetonitril, 50% posphoric acid and water 1:999; [B] phosphoric acid and Ac...

Famciclovir Tablets USP Monograph Method using Purospher STAR RP-8 endcapped HPLC column and UV Detection

Famciclovir is an antiviral drug indicated for the treatment of herpes zoster, herpes simplex virus 2 (genital herpes), herpes labialis (cold sores), etc. It is a guanosine analogue, a prodrug form of penciclovir, and marketed by Novartis under the trade name Famvir. Generics are produced by TEVA an...

Related Substances of Sildenafil Citrate (USP)

Sildenafil, sold under the brand name Viagra, among others, is a medication used to treat erectile dysfunction and pulmonary arterial hypertension. In 1994, Pfizer filed a patent covering the use of sildenafil to treat erectile dysfunction. This patent will expire in 2019. Ascentis® Express C18 colu...

HPLC Analysis of Ivermectin Injection as per USP/BP on Chromolith® High Resolution RP-18e

column Chromolith® High Resolution RP-18 endcapped, 100 x 4.6 mm (1.52022.0001) column temp. ambient mobile phase [A] acetonitrile; [B] methanol: [C] water; (106:55:39, A:B:C) flow rate 1.5 mL/min sample 0.4 mg / ml in diluent injection 10 μL detector 245 nm cell: 10 μL Featured Industry Pharmaceuti...

HPLC Analysis of Vinblastine Sulfate on Chromolith® HighResolution RP-18 endcapped 150 x 4.6mm

sample preparation Transfer USP Vincristine Sulfate RS ( or Assay RS) to a suitable volumetric flask, and dissolve in the Standard solution. column Chromolith® HighResolution RP-18 endcapped, 150 x 4.6 mm (Merck p/n 1.52023) (1.52023.0001) column temp. ambient mobile phase [A] Dimethylamine and wate...

Organic Impurity Profiling Method for Desvenlafaxine Extended Release Tablets Following the United States Pharmacopeia Monograph

Desvenlafaxine also known as O-desmethylvenlafaxine, is a synthetic form of the major active metabolite of venlafaxine. It is an antidepressant drug of the serotonin-norepinephrine reuptake inhibitor (SNRI) class and primarily used in the treatment of major depressive disorder. It was developed by P...

HPLC Analysis of Finasteride Tablets on Chromolith® HighResolution RP-18 endcapped 50 x 4.6mm

sample preparation Dissolve an accurately weighed quantity of USP Finasteride RS in Diluting solution, and dilute quantitatively, and stepwise if necessary, with Diluting solution to obtain a solution having a known concentration of about 100 μg per mL. column Chromolith HighResolution RP-18 endcapp...

HPLC Analysis of Finasteride Tablets on Chromolith® HighResolution RP-18 endcapped 100 x 4.6mm

sample preparation Dissolve an accurately weighed quantity of USP Finasteride RS in Diluting solution, and dilute quantitatively, and stepwise if necessary, with Diluting solution to obtain a solution having a known concentration of about 100 μg per mL. column Chromolith HighResolution RP-18 endcapp...

HPLC Analysis of Albuterol Tablets - Assay on Chromolith® HighResolution RP-18 endcapped 150 x 4.6mm

sample preparation 120 μg/mL of Albuterol Sulfate RS prepared as follows. Transfer USP Albuterol Sulfate RS to a suitable volumetric flask, and add a volumne of Solution A corresponding to 60% of the flask volume. Sonicate for 5 minutes, and dilute with Methanol to volume. column Chromolith® HighRes...

HPLC Analysis of Ganciclovir Injection Solution on Chromolith® HighResolution RP-18 endcapped 100 x 4.6mm

sample preparation Transfer 20.0 mL of standard stock solution (250 μg/mL of USP Ganciclovir RS in water) and 10.0 mL of the Internal standard solution to a 100 mL volumetric flask. Dilute with mobile phase to volume. column Chromolith HighResolution RP-18 endcapped, 100 x 4.6 mm (1.52022.0001) colu...

HPLC Analysis of Albuterol Tablets - Assay on Chromolith HighResolution RP-18 endcapped 50 x 4.6mm

sample preparation 120 μg/mL of Albuterol Sulfate RS prepared as follows. Transfer USP Albuterol Sulfate RS to a suitable volumetric flask, and add a volumne of Solution A corresponding to 60% of the flask volume. Sonicate for 5 minutes, and dilute with Methanol to volume. column Chromolith® HighRes...

HPLC Analysis of Albuterol Tablets - Assay on Chromolith HighResolution RP-18 endcapped 100 x 4.6mm

sample preparation 120 μg/mL of Albuterol Sulfate RS prepared as follows. Transfer USP Albuterol Sulfate RS to a suitable volumetric flask, and add a volumne of Solution A corresponding to 60% of the flask volume. Sonicate for 5 minutes, and dilute with Methanol to volume. column Chromolith® HighRes...

HPLC Analysis of Alfuzosin and Related Substances on Chromolith® HighResolution RP-18 endcapped

sample preparation Dissolve 40.0 mg of the sample in 100 mL of mobile phase. 0.4 mg/mL (400 ppm) of USP Alfuzosin System Suitability Mixture RS in Mobile phase column Chromolith® HighResolution RP-18 endcapped, 150 x 4.6 mm (1.52023.0001) column temp. 25 °C mobile phase [A] 5 ml of perchloric acid i...

TLC Silica Gel 60G Plates

Fully Compliant with International Pharmacopoeia Traditionally, TLC monographs in pharmacopoeia refer to products using silica G, containing gypsum as binder, or silica H with no foreign binder. There are about 200 monograph methods in the European Pharmacopoeia (Ph Eur) referring to these plates*. ...

Determination of Water Content in Esomeprazole Using Karl Fischer Titration

Pharmaceuticals Pharmaceuticals Pharmaceutical products are often characterized by complex formulations. Difficulties observed during Karl Fischer determination are often caused by the limited solubility. In some cases side reactions have to be considered. In dependance of composition and properties...

Determination of Water Content in Suppositories Using Karl Fischer Titration

Pharmaceuticals Pharmaceuticals Pharmaceutical products are often characterized by complex formulations. Difficulties observed during Karl Fischer determination are often caused by the limited solubility. In some cases side reactions have to be considered. In dependance of composition and properties...