R-95 HPLC/MS Grade Rhamnolipids

Rhamnolipids (L510025) are some of the best known and most often tested biosurfactants. To date, however, laboratory-grade rhamnolipids have not been available commercially. Recently, AGAE Technologies has developed a process to produce R-95 HPLC/MS Grade rhamnolipids. R-95 rhamnolipids are glycolipid biosurfactants that are microbially produced by P. aeruginosa as anionic amphiphilic molecules, with (hydrophilic) mono- or di-rhamnose sugar heads and (lipophilic) ß-hydroxyalkanoic acid tails of varying lengths.


A rhamnolipid biosurfactant is highly biodegradable, non-toxic, sustainable, and will support eco-certification as an ingredient. R-95™ comes in dry format which is 95% pure and dissolves easily. Rhamnolipids may significantly outperform synthetic surfactants in surface and interfacial tension properties and Critical Micelle Concentration, and also can work at extremes of pH, salinity and temperature. Rhamnolipids provide excellent foaming/wetting properties. It forms stable microemulsions and is soluble under high alkalinity. Also, rhamnolipid biosurfactants support sustainable ingredient and green labeling product initiatives.

Figure 1. HPLC chromatogram of the representative R-95 Rhamnolipids.


  • Pharmaceutical and cosmeceutical formulations
    Rhamnolipids function as excipients for bioavailability enhancement, solubilization or emulsification; and/or as active ingredients.1 Their mild and skin-friendly attributes, allow it to be used in personal care applications for skin, hair and facial care.
  • Environmental remediation of hydrocarbons, heavy metals and pesticides in soil and water2
    Rhamnolipids adsorb to soil and solubilize hydrocarbon or other hydrophobic molecules, increasing their bioavailability and helping removal through biodegradation, washing, or collection.
  • Antibacterial
    Rhamnolipids exert toxicity on cell membrane permeability, and are proven effective against Mycobacterium tuberculosis, Gram-negative bacteria P. aeruginosa, Enterobacter aerogenes, Serratia marcescens and Klebsiella pneumonia, and Gram-positive Micrococcus sp., Streptococcus sp., Staphylococcus sp. and Bacillus sp.
  • Antifungal
    Rhamnolipids directly lyse zoospores and they have been demonstrated effective against 23 species of fungus,3 and as an algicide, against several common species of harmful algae bloom.4


  1. Gharaei-Fathabad, E. Am. J. Drug Discovery Dev. 2010, 1,58.
  2. Mulligan, C. Environ. Pollut. 2004, 14, 372.
  3. Dorey, S. et al. Int. J. Mol. Sci. 2010, 11, 5095.
  4. Hultberg, M. et al. Harmful Algae 2008, 8, 857.