Biotin Phosphoramidite

Biotin-labeled oligonucleotides have become extremely popular in DNA capture and detection applications, due to their very high sensitivity and strong, specific binding affinity with avidin and streptavidin proteins. Biotin-based detection methods are widely used by researchers. Biotin labeling is compatible with PCR and most hybridization techniques.

Key Features of the Biotin Phosphoramidite
  • Behaves like any standard amidite on the DNA synthesizer
  • Readily soluble in acetonitrile
  • Biotin compound has a dimethozytrityl (DMT) — group on the ring structure, which allows coupling-yield determination and trityl-selective purification
  • A long, water-compatible linker arm ensures maximum sensitivity
  • The labeled oligonucleotide is ready for use in most applications after the evaporation of ammonia. Standard procedures can be used if additional purification is required
  • Provides a coupling efficiency of 95%

Product #

Structure

Description

Formula

Compatibility

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M042000 Biotin Phosphoramidite C46H64N5O8PS    
M042030 Biotin Phosphoramidite C46H64N5O8PS configured for ABI
 
M042080 Biotin Phosphoramidite C46H64N5O8PS configured for PerkinElmer
configured for Polygen
 
M042100 DMT-Biotin-C6 Phosphoramidite C46H64N5O6PS    
M042180 DMT-Biotin-C6 Phosphoramidite C46H64N5O6PS configured for PerkinElmer
configured for Polygen
 
M042130 DMT-Biotin-C6 Phosphoramidite C46H64N5O6PS configured for ABI