Fluorescein Phosphoramidite

Fluorescein phosphoramidite is composed of a protected fluorescein molecule linked to a ß-cyanoethylphosphoramidite. The fluorescein derivative consists of to isomers derived form 5- and 6- carboxy fluorescein. As a result, two peaks will be seen on a RP-HPLC chromatogram of the synthesis product.

Key Features of the Fluorescein Phosphoramidite
  • Readily soluble in acetonitrile
  • Couples to the 5’ end of the oligonucleotide using standard synthesis protocols
  • Protecting groups on the fluorescein moiety are removed under standard conditions of cleavage and deprotection with concentrated ammonia
  • A detritylation step is not required since the fluorescein phosphoramidite does not contain a dimethoxytrityl (DMT-) group
  • The labeled oligonucleotide is ready for use in most applications after the evaporation of ammonia. Standard procedures can be used if additional purification is required
  • The fluorescein moiety provides a purification handle in RP-HPLC purification

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M041080 Fluorescein Phosphoramidite C46H58N3O10P configured for PerkinElmer
configured for Polygen