Stable Isotope Labeled Cell Free Protein Expression

Products for Cell Free Synthesis of Stable Isotope Labeled Proteins

Cell Free Protein Expression enables the production of proteins in solution. These in vitro protein expression (iPE) kits incorporate a proprietary, advanced cell-free protein synthesizing technology that reduces isotope dilution when used to synthesize Stable Isotope Labeled (SIL) proteins. These systems utilize E. coli cell extract and facilitate easy and efficient protein expression with the addition of circular or linear DNA.

The kits include natural-abundance amino acids. For production of Stable Isotope Labeled proteins, SIL amino acid mixtures are available for separate purchase (select desired labeling - 13C, 15N, D).

In Vitro Protein Expression Kits

Product #

Description

Add to Cart

905089 In Vitro Protein Expression (iPE) Kit II
797006 In Vitro Protein Expression (iPE-SS) Kit for disulfide-containing proteins
767824 iPE-Quick Kit

SAIL Amino Acids

Product #

Structure

Description

Isotopic Purity

Add to Cart

900911 L-Isoleucine-13C,d7 (2,3,4,4-d4-3-methyl-d3, 5-13C) ≥95 atom % D
≥97 atom % 13C
900908 L-Leucine-13C, d7 (2,3,3,4,5,5,5-d7, pro-R-methyl-13C) ≥95 atom % D
≥97 atom % 13C
900795 L-Leucine-13C, d7 (2,3,3,4,5,5,5-d7, pro-S-methyl-13C) ≥95 atom % D
≥97 atom % 13C
900792 L-Leucine-13C,d7,15N (2,3,3,4,5,5,5-d7, pro-S-methyl-13C) ≥95 atom % 15N
≥95 atom % D
≥97 atom % 13C
900796 L-Threonine-13C,d2,15N (2,3-d2, 3-methyl-13C) ≥95 atom % 13C
≥95 atom % 15N
≥95 atom % D
900909 L-Valine-13C, d5 (2,3,4,4,4-d5, pro-R-methyl-13C) ≥90 atom % D
≥97 atom % 13C
900793 L-Valine-13C,d5,15N (2,3,4,4,4-d5, pro-R-methyl-13C) ≥95% 15N
≥95 atom % D
≥97 atom % 13C
900910 L-Valine-13C,d5 (2,3,4,4,4-d5, pro-S-methyl-13C) ≥90 atom % D
≥97 atom % 13C

Stable Isotope Labeled (SIL) Cell Free Amino Acid Mixtures

Product #

Description

Add to Cart

767964 Cell Free Amino Acid Mixture - 13C,15N 98 atom % 15N, 98 atom % 13C, ~5-100 mM in water
767972 Cell Free Amino Acid Mixture-15N 98 atom % 15N, ~5-100 mM in water
771031 Cell Free Amino Acid Mixture-13C, 15N, D 97 atom % D, 98 atom % 13C, 98 atom % 15N, ~2-100 mM in water