HPLC

HPLC方法转换计算器

 

计算从一个HPLC色谱柱改变到另一个HPLC色谱柱的等度洗脱或梯度洗脱方法的条件。方法允许范围涵盖微径柱到制备色谱柱一系列参数的计算。计算节省的时间和溶剂。

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Current Method
New Method
New Methodadjust flow rate
Column Length(cm)
Column I.D.(mm)
Particle Size(μm)
Flow Rate(mL/min)
0.35
Injection Volume(μL)
1.4
1.4
Pressure(psi/bar)
1543.2
1332.8
Run Time (min)
6.00
6.95
Time Saved (min)
24.0
23.1
Solvent Saved (mL)
27.9
27.9
sigma-aldrich

HPLC Method Transfer Calculator

Current Method
New Method
New Methodadjust flow rate
Column Length(cm)
Column I.D.(mm)
Particle Size(μm)
Flow Rate(mL/min)
0.35
Injection Volume(μL)
1.4
1.4
Pressure(psi/bar)
1543.2
1332.8
Run Time (min)
6.00
6.95
Equilibration
Time(min)
17.44
3.49
4.04
Time Saved (min)
24.0
23.1
Solvent Saved (mL)
27.9
27.9

Gradient
Conditions
Current Method
New Method
New MethodAdjust Dwell Volume
Dwell Volume(mL)
Gradient Delay(min)
0.00
0.00

Gradient
Table
TIME
% A
% B
New Time
New TimeAdjusted Flow
20
0.00
0.00
20
0.40
0.46
50
2.00
2.32
50
4.00
4.63
20
5.00
5.79
20
6.00
6.95


sigma-aldrich

HPLC Method Transfer Calculator

The scaled gradient method is based on estimates of column volumes and is therefore only an approximation. More precise method scaling requires actual measured values of column volumes. Column volume is a function of particle size, particle structure, and column packing.

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