BIS-TRIS

BIS-TRIS是一种在结构上非常类似于Trizma(“Tris”)的胺缓冲液,是蛋白质和核酸体系的重要缓冲液,也被用作二甲胂酸缓冲体系的替代品1
可用pH范围 5.8 - 7.2
工作浓度范围 10mM-50mM5-7

  

        不同等级或质量层级的产品比较
名称 CAS 分子式 分子量 试剂级 生命科学级 制药级  溶液
BIS-TRIS 6976-37-0 C8H19NO5 209.24 RDD013 B9754 B7535 14879
B4429
RES1161B-A7 PHG0004  
BIS-TRIS盐酸 124763-51-5 C8H19NO5 • HCl 245.70 B6032   RES1164B-A7  

互作信息

BIS-TRIS可与Cu及Pb结合,与Mg, Ca, Mn, Co, Ni, Zn及Cd之间存在弱作用关系4

    

参考文献

  1. Scopes, R.K. ed. (1994) Protein Purification (Principles and Practice) 3rd Edition, p. 352; Springer-Verlag.
  2. Sheller, Kurt H., et al. European Journal of Biochemistry. 107, 455-466 (1980)
  3. Sheller et. al, 2005 European Journal of Biochemistry (2005)
  4. Ferreira CM, Pinto IS, Soares, EV, Soares HM. 2015 Royal Society of Chemistry 30989- 31003
  5. Celis JE, Cell biology: a laboratory handbook v2, Elseveir Academic Press, 2006 pp.161
  6. Perutz, Science is not a quiet life: Unravelling the atomic mechanism of haemoglobin, World Scientific Publishing, 1997
  7. Kebede N, Francis PS, Barbante GJ, Hogan CF, Electrogenerated chemiluminescence of tris (2,2’ biopyridine) ruthenium (II) using common biological buffers as co-reactant, pH buffer and supporting electrolyte, Royal Society of Chemistry 2015(140) pp7142-7145