生物学缓冲液

Trizma® HCl

 

Sigma-Aldrich® Trizma® HCl 物理属性
Synonyms TRIS HCl
TRIS 盐酸
三(羟甲基)氨基甲烷盐酸
Trizma® HCl Image
分子式 NH2C(CH2OH)3 · HCl Beilstein 注册号 3675235 可用pH范围 7.0 - 9.0
分子量 157.60 MDL 号 MFCD00012590 pKa(25度时) 8.1
CAS 号 1185-53-1 EG/EC 号 214-684-5    

 

Sigma-Aldrich Trizma HCl 参数比较
  T3253 T5941 T6666 93287 93363
级别 试剂级,≥99.0%(氧化还原滴定),结晶 用于分子生物学, BPC,经细胞培养测试,≥99.0%(滴定) BioXtra, pH 3.5 - 5.0 (20 °C, 0.5 M H2O溶液), ≥99.0% (滴定) BioUltra,用于荧光,≥99.0% (AT) BioUltra, 用于分子生物学,
≥99.0% (AT)
纯度 ≥99.0% ≥99.0% ≥99.0% ≥99.0% ≥99.0%
试剂检测
  T3253 T5941 T6666 93287 93363
增强的质量分析缓冲液 X X X    
λex       200 - 600 nm  
λem       300 - 800 nm  
260NM   ≤0.06 ≤0.015 ≤0.12 ≤0.12
280NM   ≤0.06 ≤0.010 ≤0.08 ≤0.08
290NM ≤0.05 ≤0.05      
IR X X X    
pH     3.5 - 5.0 (25 °C,
0.5 M in H2O)
2.5 - 4.0 (25 °C, 4 M in H2O) 2.5 - 4.0 (25 °C, 4 M in H2O)
Karl Fischer ≤0.5% ≤0.5%      
LOD     ≤0.2% ≤0.2% ≤0.2%
不容物     过滤检测 过滤检测 过滤检测
炽灼残渣     ≤0.2% ≤0.2% ≤0.2%
生命科学检测
  T3253 T5941 T6666 93287 93363
内毒素   ≤0.1 eu/mg      
SPC   ≤100 cfu/g      
细胞检测   X      
RNA酶   X     X
DNA酶   X     X
切口酶   X      
蛋白酶   X     X
磷酸化酶         X
电泳适用性   X      
痕量元素杂质
  T3253 T5941 T6666 93287 93363
钠(Na)     ≤0.005% ≤0.0050% ≤0.0050%
硫酸根(SO4     ≤0.005% ≤0.0050% ≤0.0050%
铝(Al)     ≤0.0005% ≤0.0005% ≤0.0005%
砷(As)     ≤0.0001% ≤0.00001% ≤0.00001%
钡(Ba)     ≤0.0005% ≤0.0005% ≤0.0005%
铋(Bi)     ≤0.0005% ≤0.0005% ≤0.0005%
钙(Ca)     ≤0.001% ≤0.0010% ≤0.0010%
镉(Cd)     ≤0.0005% ≤0.0005% ≤0.0005%
钴(Co)     ≤0.0005% ≤0.0005% ≤0.0005%
铬(Cr)     ≤0.0005% ≤0.0005% ≤0.0005%
铜(Cu)     ≤0.0005% ≤0.0005% ≤0.0005%
铁(Fe)     ≤0.0005% ≤0.0005% ≤0.0005%
钾(K)     ≤0.005% ≤0.0050% ≤0.0050%
锂(Li)     ≤0.0005% ≤0.0005% ≤0.0005%
镁(Mg)     ≤0.0005% ≤0.0005% ≤0.0005%
锰(Mn)     ≤0.0005% ≤0.0005% ≤0.0005%
钼(Mo)     ≤0.0005% ≤0.0005% ≤0.0005%
镍(Ni)     ≤0.0005% ≤0.0005% ≤0.0005%
铅(Pb) ≤0.0005% ≤0.0005% ≤0.0005% ≤0.0005% ≤0.0005%
锶(Sr)     ≤0.0005% ≤0.0005% ≤0.0005%
锌(Zn)     ≤0.0005% ≤0.0005% ≤0.0005%