慢病毒细胞系

采用慢病毒递送shRNA,已经成为许多研究RNAi的实验室的标准操作流程。采用VSV-G假型慢病毒感染(转导),可以将shRNA递送到广泛类型的细胞中,包括:未分裂期细胞、原代细胞和干细胞。即便在极少数难以转导的某些细胞中,也有多种方法可以增强递送效果,如: Spinoculation法或磁性纳米微粒珠(ExpressMag™转导系统)。

本表概述
下表旨在帮助您完成各种指定细胞类型的转导实验。所有经验证的细胞系均为研究人员采用RNAi联盟(TRC) shRNA文库,通过慢病毒应用的成果。如果您未找到自己的目标细胞系,建议选择类似的细胞系作为自行开展研究的出发点。在本表中列出了一到两种转导条件。

许多细胞直接关联PubMed参考文献,左键单击箭头内任意位置或单击NCBI链接细胞直接访问。一个细胞类型可能存在多个参考资源。建议评审多个参考资源以便为您的细胞模型确定最适合的转导条件。

某些具有特殊转导条件的细胞已经列在表中。这些条件由博德研究所、Sigma® 研发部或外部合作单位实验确定。但这些条件对于指定的研究人员有效,仅可作为您开展工作的起点。

如果慢病毒来源于Sigma MISSION® 团队或TRC-博德研究所,则其滴度为10e6至10e7。


 

细胞名称 PubMed 或来源 器官/组织 癌症/细胞类型 生物体 接种密度(1000 细胞 / cm^2) MOI (x) 或病毒体积 (µl/1000 细胞) 嘌呤霉素(µg/ml) 注意
Phoenixamphotropic 18006659 - - - - - - -
PSR-1E 17360431 - - - - - - -
SC21 18372920 - - - - - - -
siCandsiPML2 16873256 - - - - - - -
Macrophages 19237602 Blood - H. sapiens - - - -
CL22 18372920 Epidermis - M. musculus - - - -
Gingival epithelial cells 18624948 Epithelial - - - - - -
HF 16873256 Foreskin - H. sapiens - - 2.0 -
HaCAT 18794124 Keratinocyte - H. sapiens - - 10.0 -
BE R 18070930 Kidney - M. auratus - - - -
LLC-PK1 18474214 Kidney - S. domestica - - - -
MDCK 18172008 Kidney - C. Lupus familliarus - - 4.0 -
HMECtert 17360431 Mammary - H. sapiens - - - -
5A97 17936323 Mesothelium - H. sapiens - - - -
5ADL 17442711 Mesothelium - H. sapiens - - - -
Mouse Muscle Cells 18552838 Muscle - M. musculus - - - -
astrocytoma samples 18398503 Nervous system - - - - - -
embryonic cortical neuroepithelium 17409236 Nervous system - M. musculus - - - -
MN 17409236 Nervous system - - - 10 x - -
GH3 17706597 Pituitary - R. norvegicus - - - -
CE 18441300 Retina - H. sapiens - - - -
BxPC-3 18316571 Pancreas, epithelial Adenocarcinoma H. sapiens - 10 x - -
BxPC-3 18403640 Pancreas, epithelial Adenocarcinoma H. sapiens - - - -
MCF-7 17384642 Breast Adenocarcinoma H. sapiens - - - -
MCF-7 17535848 Breast Adenocarcinoma H. sapiens - - - -
MCF-7 17574021 Breast Adenocarcinoma H. sapiens - - - -
MCF-7 18948598 Breast Adenocarcinoma H. sapiens - - - -
MCF-7 19015320 Breast Adenocarcinoma H. sapiens - - - -
MCF-7 19061500 Breast Adenocarcinoma H. sapiens - - - -
MCF-7 Sigma MISSION Team Breast Adenocarcinoma H. sapiens 47.5 2 x 1.0 -
MCF-7 TRC-Broad Institute Breast Adenocarcinoma H. sapiens 3.1 8.0 µl/1000 cells 2.0 Utilize Spinfection
MDA-MB-231 18403640 Breast Adenocarcinoma H. sapiens - - - -
MDA-MB-231 19014680 Breast Adenocarcinoma H. sapiens - - - -
MDA-MB-231 Sigma MISSION Team Breast Adenocarcinoma H. sapiens 62.5 1 x 0.5 -
MDA-MB-468 18403640 Breast Adenocarcinoma H. sapiens - - - -
COLO205 17957250 Colon Adenocarcinoma H. sapiens - - - -
DLD-1 17906625 Colon Adenocarcinoma H. sapiens - - - -
DLD-1 18794900 Colon Adenocarcinoma H. sapiens - - - -
HT-29 17141160 Colon Adenocarcinoma H. sapiens - 3 x 2.0 -
HT-29 18403640 Colon Adenocarcinoma H. sapiens - - - -
HT-29 19014680 Colon Adenocarcinoma H. sapiens - - - -
HT-29 Sigma MISSION Team Colon Adenocarcinoma H. sapiens 32.0 3 x 2.0 -
SW480 19014680 Colon Adenocarcinoma H. sapiens - - - -
SK-HEP-1 18403640 Liver Adenocarcinoma H. sapiens - - - -
Calu-3 15731348 Lung Adenocarcinoma H. sapiens - - - -
H1703 18427549 Lung Adenocarcinoma H. sapiens - - - -
NCI-H838 18403640 Lung Adenocarcinoma H. sapiens - - - -
PC9 18711136 Lung Adenocarcinoma H. sapiens - - - -
PC9 19079597 Lung Adenocarcinoma H. sapiens - - - -
PC9NSCLC 18591665 Lung Adenocarcinoma H. sapiens - - - -
ES-2 17942676 Ovary Adenocarcinoma H. sapiens - - - -
OVCAR-3 17311912 Ovary Adenocarcinoma H. sapiens - - - -
OVCAR-3 17942676 Ovary Adenocarcinoma H. sapiens - - - -
SKOV-3 15252144 Ovary Adenocarcinoma H. sapiens - - - -
SKOV-3 17942676 Ovary Adenocarcinoma H. sapiens - - - -
SKOV-3 17942676 Ovary Adenocarcinoma H. sapiens - - - -
SKOV-3 Sigma MISSION Team Ovary Adenocarcinoma H. sapiens 31.3 15 x - -
SKOV-3 Sigma MISSION Team Ovary Adenocarcinoma H. sapiens 31.3 15 x - ExpressMag Enhanced
SK-OV-3 18403640 Ovary Adenocarcinoma H. sapiens - - - -
ACHN 18948597 Renal Adenocarcinoma H. sapiens - - - -
NCI-H820 19079597 Lung Adenocarcinoma, papillary H. sapiens - - - -
D54 17707275 Nervous system Astrocytoma H. sapiens - - 1.0 Polybrene
NCH492 18398503 Nervous system Astrocytoma, pilocytic H. sapiens - - 2.0 Polybrene
GM05823 18922873 - Ataxia telangiectasia H. sapiens - - - -
HS578T TRC-Broad Institute Breast Breast Cancer H. sapiens 3.1 3.8 µl/1000 cells 2.0 Utilize Spinfection
16HBE 18204074 Lung Bronchial Epithelial H. sapiens - - 1.0 -
16HBE 18204074 Lung Bronchial Epithelial H. sapiens - - - -
CRC 17906625 Colorectal Cancer H. sapiens - - 1.0 -
J82 18403640 Bladder Carcinoma H. sapiens - - - -
T24 18403640 Bladder Carcinoma H. sapiens - - - -
MDA-MB-453-I4 18353781 Breast Carcinoma H. sapiens - - - -
MMC Sigma MISSION Team Breast Carcinoma M. musculus 62.5 4 x 8.0 -
T47D 19061500 Breast Carcinoma H. sapiens - - - -
T47D TRC-Broad Institute Breast Carcinoma H. sapiens 3.1 6.0 µl/1000 cells 2.0 Utilize Spinfection
ZR-75-1 18757432 Breast Carcinoma H. sapiens - - - DNA Transient
Co-115 17906625 Colon Carcinoma H. sapiens - - - -
HCT116 17967892 Colon Carcinoma H. sapiens - - - -
HCT116 18403640 Colon Carcinoma H. sapiens - - - -
HCT116 18562296 Colon Carcinoma H. sapiens - - - -
HCT116 18794900 Colon Carcinoma H. sapiens - - - -
HCT116 19014680 Colon Carcinoma H. sapiens - - - -
HCT116 Sigma MISSION Team Colon Carcinoma H. sapiens 30.0 5 x 1.0 -
RKO 18403640 Colon Carcinoma H. sapiens - - - -
RKO 18948598 Colon Carcinoma H. sapiens - - - -
A431 15731348 Epithelial Carcinoma H. sapiens - - - -
A431 Sigma MISSION Team Epithelial Carcinoma H. sapiens 75.0 5 x 0.5 -
A498 18948597 Kidney Carcinoma H. sapiens -   - -
Hepa1-6 Sigma MISSION Team Liver Carcinoma M. musculus 32.0 3 x 2.0 -
HepG2 17449864 Liver Carcinoma H. sapiens - 10 x - -
HepG2 17687072 Liver Carcinoma H. sapiens - - - -
HepG2 17993646 Liver Carcinoma H. sapiens - - 2.0 Polybrene
HepG2 18403640 Liver Carcinoma H. sapiens - - - -
HepG2 Sigma MISSION Team Liver Carcinoma H. sapiens 32.0 3 x 2.0 -
A549 15731348 Lung Carcinoma H. sapiens - - - -
A549 16982682 Lung Carcinoma H. sapiens - - - -
A549 17535848 Lung Carcinoma H. sapiens - - - -
A549 18337815 Lung Carcinoma H. sapiens - - - -
A549 18337823 Lung Carcinoma H. sapiens - - - -
A549 18403640 Lung Carcinoma H. sapiens - - - -
A549 18768981 Lung Carcinoma H. sapiens - - - -
A549 18948595 Lung Carcinoma H. sapiens - - - -
A549 Sigma MISSION Team Lung Carcinoma H. sapiens 30.0 5 x 2.0 -
A549 Sigma MISSION Team Lung Carcinoma H. sapiens 46.9 5 x 1.0 -
A549 TRC-Broad Institute Lung Carcinoma H. sapiens 1.6 6.0 µl/1000 cells 2.0 -
LLC-1 Sigma MISSION Team Lung Carcinoma M. musculus 46.9 6 x 2.0 -
NSCLC 16906227 Lung Carcinoma - - - - -
NSCLC 19079597 Lung Carcinoma - - - - -
TSA 18559453 Mammary gland Carcinoma M. musculus - - - -
TOV112D 17942676 Ovary Carcinoma H. sapiens - - - -
8988T 19050074 Pancreas Carcinoma H. sapiens - - - -
MiaPaCa 18089810 Pancreas Carcinoma H. sapiens - - - -
MiaPaCa2 17114012 Pancreas Carcinoma H. sapiens - - - -
MiaPaCa2 17178855 Pancreas Carcinoma H. sapiens - - 3.0 -
MiaPaCa2 18403640 Pancreas Carcinoma H. sapiens - - - -
DU145 17709381 Prostate Carcinoma H. sapiens - - - -
DU145 18089825 Prostate Carcinoma H. sapiens - - - -
DU145 18403640 Prostate Carcinoma H. sapiens - - - -
DU145 18838960 Prostate Carcinoma H. sapiens - - - -
PC3 17574021 Prostate Carcinoma H. sapiens - - - -
PC3 18089825 Prostate Carcinoma H. sapiens - - - -
PC3 18403640 Prostate Carcinoma H. sapiens - - - -
PC3 18838960 Prostate Carcinoma H. sapiens - - - -
LNCaP 18089825 Prostate Carcinoma H. sapiens - - - -
LNCaP 18838960 Prostate Carcinoma H. sapiens - - - -
LNCaP Sigma MISSION Team Prostate Carcinoma H. sapiens 62.5 5 x 2.0 -
786-O 18403640 Renal Carcinoma H. sapiens - - - -
786-O 18948598 Renal Carcinoma H. sapiens - - - -
786-O TRC-Broad Institute Renal Carcinoma H. sapiens 1.9 3.3 µl/1000 cells 3.5 -
HSC-5 17535848 Skin Carcinoma H. sapiens - - - -
NCI-H647 18948598 Lung Carcinoma, adenosquamous H. sapiens - - - -
H358 15731348 Lung Carcinoma, Bronchioalveolar H. sapiens - - - -
NCI-H358 18948591 Lung Carcinoma, bronchioalveolar H. sapiens - - - -
CaSki 18948591 Cervix Carcinoma, epidermoid H. sapiens - - - -
OECM-1 18213732 Keratinocyte Carcinoma, epidermoid H. sapiens - - - -
Calu-1 18948595 Lung Carcinoma, epidermoid H. sapiens - - - -
HeLa 15737938 Cervix Carcinoma, epitheloid H. sapiens - - - -
HeLa 16916793 Cervix Carcinoma, epitheloid H. sapiens - - - -
HeLa 16998475 Cervix Carcinoma, epitheloid H. sapiens - - - -
HeLa 17386266 Cervix Carcinoma, epitheloid H. sapiens - - - -
HeLa 17724024 Cervix Carcinoma, epitheloid H. sapiens - - - -
HeLa 18403640 Cervix Carcinoma, epitheloid H. sapiens - - - -
HeLa 18564371 Cervix Carcinoma, epitheloid H. sapiens - - - -
HeLa 18768981 Cervix Carcinoma, epitheloid H. sapiens - - - -
HeLa 18948591 Cervix Carcinoma, epitheloid H. sapiens - - - -
HeLa 19017811 Cervix Carcinoma, epitheloid H. sapiens - - - -
HeLa 19293187 Cervix Carcinoma, epitheloid H. sapiens - - - -
HeLa Sigma MISSION Team Cervix Carcinoma, epitheloid H. sapiens 32.0 3 x 2.0 -
WRO 17639054 Thyroid Carcinoma, Follicular H. sapiens - - - Polybrene
KATO III 18381441 Stomach Carcinoma, gastric H. sapiens - - 8.0 Polybrene
HuH-7 17272514 Liver Carcinoma, Hepatocellular H. sapiens - - 1.0 -
T84 18974311 Lung Carcinoma, metastatic H. sapiens - - - -
H3255 19079597 Lung Carcinoma, NSCLC H. sapiens - - - -
H3255 Sigma MISSION Team Lung Carcinoma, NSCLC H. sapiens 25.0 10 x 1.5 -
SAS HNSCC 18213732 - Carcinoma, squamos H. sapiens - - - -
SW579 18403640 Thyroid Carcinoma, squamous H. sapiens - - - -
SiHa 18948597 Cervix Carcinoma, squamous cell H. sapiens - - - -
HEp-2 16873258 Larynx Carcinoma, squamous cell H. sapiens - - - -
HEp-3 19279115 Larynx Carcinoma, squamous cell H. sapiens - - - -
MOSER-S 17565986 Colon Colorectal cancer H. sapiens - - - Polybrene
BM-DC 12649500 Bone Marrow Dendritic cells - - 4500 x 1.0 Polybrene
MES23.5 18980610 Nervous system Dopaminergic R. norvegicus - - - -
P6 17828296 Fibroblast, embryo Embryonic M. musculus - - - -
REF 18619965 Fibroblast, embryo Embyryonic R. norvegicus - - - DNA Transient
HUVEC 16828724 Umbilicus Endothelial cells H. sapiens - 100 x - Polybrene
HUVEC 17535848 Umbilicus Endothelial cells H. sapiens - - - -
HUVEC 18602101 Umbilicus Endothelial cells H. sapiens - - - -
HMVEC Sigma MISSION Team Endothelial Endothelial, microvascular H. sapiens 46.9 10 x 2.0 -
HMLE 18483246 Breast Epithelial H. sapiens - - - -
HMLER 18483246 Breast Epithelial H. sapiens - - - -
HC11 18836481 Mammary Epithelial M. musculus - - - -
HMEC 17535848 Mammary Epithelial H. sapiens - - - -
HMEC 17535848 Mammary Epithelial H. sapiens - - - -
HMEC 17574021 Mammary Epithelial H. sapiens - - - -
IOSE80 17942676 Ovary Epithelial M. musculus - - - -
TEC TRC-Broad Institute Thymus Epithelial H. sapiens 3.1 2.0 µl/1000 cells 2.0 Utilize Spinfection
hTERT-HME1 19112104 Mammary gland, breast Epithelial immortalized with hTERT H. sapiens - - - -
MCF-12A 17360431 Breast Epithelial spontaneous immortilization H. sapiens - - - -
MCF-10A 17360431 Breast Epithelial, non-tumorigenic H. sapiens - - - -
MCF-10A 18094039 Breast Epithelial, non-tumorigenic H. sapiens - - - -
MCF-10A 17060456 Breast Epithelial, non-tumorigenic H. sapiens - - - -
MCF-10A 18413746 Breast Epithelial, non-tumorigenic H. sapiens - - - -
MCF-10A 19112533 Breast Epithelial, non-tumorigenic H. sapiens - - - -
IEC-6 19014680 Small intestine Epithelioid R. norvegicus - - - -
HD3 18703050 - Erythroblast G. domesticus - 5 x 1.0 -
TF-1 17942397 Bone Marrow Erythroleukemia H. sapiens - - - -
HEL 17347684 Embryonic Kidney Erythroleukemia, AML M6 H. sapiens - - - -
F02-98 18922873 - Fibroblast H. sapiens - - - -
FR3t3 17692050 - Fibroblast R. norvegicus - - - -
MCH 58 18940309 - Fibroblast H. sapiens - - - -
MCH 58 18940309 - Fibroblast H. sapiens - - - -
MCF-7-ras 15882617 Breast Fibroblast H. sapiens - - - -
CL1 18838960 Embryo Fibroblast C. griseus - - - -
BHK-21 18768981 Kidney Fibroblast M. auratus - - - -
NIH-3T3 19293187 Embryo, NIH Swiss Fibroblast, adherent monolayer M. musculus - - - -
MRC-5 17581253 Lung, embryonic Fibroblast-like H. sapiens - - - -
WI-38 18006659 Lung, fetal Fibroblast-like H. sapiens - - - -
WI-38 18006659 Lung, fetal Fibroblast-like H. sapiens - - - -
WI-38 18948595 Lung, fetal Fibroblast-like H. sapiens - - - -
COS-7 17550976 Kidney Fibroblast-like C. aethiops - - - DNA Transient
COS-7 17684014 Kidney Fibroblast-like C. aethiops - - - -
HFFF2 18922870 Foreskin Fibroblasts H. sapiens - - - -
HLF 12796781 Lung Fibroblasts H. sapiens - - - -
IMR-90 17082179 Lung Fibroblasts H. sapiens - - - -
IMR-90 17082179 Lung Fibroblasts H. sapiens - - - -
IMR-90 18006659 Lung Fibroblasts H. sapiens - - - -
IMR-90 18006659 Lung Fibroblasts H. sapiens - - - -
MRC-5 Sigma MISSION Team Lung, embryonic Fibroblasts H. sapiens 20.8 1 x 2.0 -
2091 17082179 Foreskin Fibroblasts, early passage H. sapiens - - - -
C3H10T1/2 17183530 Fibroblast Fibroblasts, embryo M. musculus - - 5.0 -
MEF 16951254 Connective Fibroblasts, embryonic M. musculus - - 1.0 -
MEF 17386266 Connective Fibroblasts, embryonic M. musculus - - - -
MEF 18250155 Connective Fibroblasts, embryonic M. musculus - - - -
MEF 19112104 Connective Fibroblasts, embryonic M. musculus - - - -
MEF 19150980 Connective Fibroblasts, embryonic M. musculus - - - -
MEF Sigma MISSION Team Connective Fibroblasts, embryonic M. musculus 46.9 16 x 3.0 -
MEF Sigma MISSION Team Connective Fibroblasts, embryonic M. musculus 46.9 8 x 3.0 ExpressMag Enhanced
MCF-10A-2A 17360431 Breast Fibrocystic disease H. sapiens - - - -
HT-1080 18518968 Fibroblast Fibrosarcoma H. sapiens - - - -
HT-1080 18518968 Fibroblast Fibrosarcoma H. sapiens - - - Polybrene
HT-1080 19293187 Fibroblast Fibrosarcoma H. sapiens - - - -
T98G 19293187 Epithelial Glioblastoma H. sapiens - - - -
A172 17060456 Nervous system Glioblastoma H. sapiens - - - -
FINE 0308 TRC-Broad Institute Nervous system Glioblastoma H. sapiens 3.1 5.0 µl/1000 cells 0.4 Utilize Spinfection
GBMlines 18394558 Nervous system Glioblastoma H. sapiens - - - -
Frozen tumor 18394558 Nervous system Glioblastoma, Grade IV H. sapiens - - - -
U343MG 17828296 Nervous system Glioblastoma-astrocytoma H. sapiens - - - -
U373 18189330 Nervous system Glioblastoma-astrocytoma H. sapiens - - - -
U-87MG 18403640 Nervous system Glioblastoma-astrocytoma H. sapiens - - - -
CD133+ Glioma Cells 18676824 Nervous system Glioma - - - - -
LN308 17075581 Nervous system Glioma H. sapiens - - - -
U118-MG 19293187 Nervous system Glioma, malignant H. sapiens - - - -
MN9D 18434601 Mesencephalon Hybridoma fusion of mesencephalon with neuroblastoma M. musculus - - 5.0 -
MN9D 18980610 Mesencephalon Hybridoma fusion of mesencephalon with neuroblastoma M. musculus - - - -
JSL-1 17210637 Blood Jurkat-derived H. sapiens - - - -
JSL-1 18669861 Blood Jurkat-derived H. sapiens - - - -
JSL-1 18669861 Blood Jurkat-derived H. sapiens - - 5.0 Polybrene
NHEK 18927586 Epidermis Keratinocytes, non-tumorigenic H. sapiens - - - -
HFK 18438432 Foreskin Keratinocytes, non-tumorigenic H. sapiens - - - -
P12 17828296 Blood Leukemia H. sapiens - - - -
HL-60 17347684 Blood, peripheral Leukemia H. sapiens - - - -
CLL 17200714 Lymph Leukemia H. sapiens - - - -
CMK 17347684 Blood, peripheral Leukemia, acute megakaryocytic H. sapiens - - - -
MV4-11 17347684 Blood Leukemia, acute monocytic H. sapiens - - - -
MONO-MAC-6 17347684 Blood, peripheral Leukemia, acute monocytic H. sapiens - - - -
AML 18068628 Blood Leukemia, acute myeloid H. sapiens - - - -
Nomo TRC-Broad Institute Blood Leukemia, acute myeloid H. sapiens 25.0 1.3 µl/1000 cells 2.0 Utilize Spinfection
SKM-1 17347684 Blood Leukemia, acute myeloid H. sapiens - - - -
SKM-1 17347684 Blood Leukemia, acute myeloid H. sapiens - - 2.0 Polybrene
THP-1 17347684 Blood Leukemia, acute myeloid H. sapiens - - - -
THP-1 TRC-Broad Institute Blood Leukemia, acute myeloid H. sapiens 34.4 1.1 µl/1000 cells 0.5 Utilize Spinfection
Kasumi-1 17347684 Blood, peripheral Leukemia, acute myeloid H. sapiens - - - -
KG-1 17347684 Blood Leukemia, acute myeloid H. sapiens - - - -
EOL-1 17347684 Blood, peripheral Leukemia, acute myeloid (eosinophilic) H. sapiens - - - -
NB4 17347302 Blood Leukemia, acute promyelocytic H. sapiens - 10 x 2.0 Polybrene
NB4 17347684 Blood Leukemia, acute promyelocytic H. sapiens - - - -
NB4 19144990 Blood Leukemia, acute promyelocytic H. sapiens - - - -
Jurkat 17616514 Blood Leukemia, Acute T Cell H. sapiens - - - -
Jurkat 19066270 Blood Leukemia, Acute T Cell H. sapiens - 10 x 2.0 Utilize Spinfection
Jurkat Sigma MISSION Team Blood Leukemia, Acute T Cell H. sapiens 20.8 10 x 2.0 Utilize Spinfection
K-562 17347684 Pleural effusion Leukemia, chronic myeloid H. sapiens - - - -
7T-ALLlines 17114293 T cell Leukemia, lymphoblastic H. sapiens - - - -
LK63 18385452 Blood Leukemia, lymphoblastic H. sapiens - - - -
LK63 18385452 Blood Leukemia, lymphoblastic H. sapiens - - - -
CEM 18509086 Blood Leukemia, lymphoblast-like H. sapiens - - - -
CEM 18509086 Blood Leukemia, lymphoblast-like H. sapiens - - 2.0 Polybrene
NOMO-1 17347684 Blood Leukemia, myeloid H. sapiens - - - -
MOLM-14 17347684 Blood, peripheral Leukemia, myeloid H. sapiens - - - -
ALCL 17111047 Bone Marrow Lymphoma H. sapiens - - - -
p53 ERlymphoma 17235397 Bone Marrow Lymphoma - - - - -
EW36 18066065 Bone Marrow Lymphoma, B-Cell H. sapiens - - - -
BJAB 18669861 Bone Marrow Lymphoma, Burkitt's H. sapiens - - - -
BL41 18669861 Bone Marrow Lymphoma, Burkitt's H. sapiens - - - -
HuT78 18509086 Blood, peripheral Lymphoma, cutaneous, T cell H. sapiens - - - -
U-937 17347684 Pleural effusion Lymphoma, histiocytic H. sapiens - - - -
RAWclone6 18971331 - Macrophage M. musculus - - - -
CGNPs 18755774 Nervous system Medulloblastomas H. sapiens - - - -
DFB 17828296 Skin Melanoma H. sapiens - - - -
LiBr 18385452 Skin Melanoma H. sapiens - - - -
MM200 18701495 Skin Melanoma H. sapiens - 5 x 2.0 Polybrene
A-375 18403640 Epithelial Melanoma, malignant H. sapiens - - - -
A2058 18403640 Epithelial Melanoma, metastatic H. sapiens - - - -
B16-F10 18981472 Epithelial Melanoma, metastatic M. musculus - - - -
B16-F10 18981472 Epithelial Melanoma, metastatic M. musculus - - - -
B16-F10 Sigma MISSION Team Epithelial Melanoma, metastatic M. musculus 46.9 5 x 2.0 -
MM-AN Sigma MISSION Team Skin Melanoma, metastatic H. sapiens 62.5 16 x 6.0 -
MM-AN Sigma MISSION Team Skin Melanoma, metastatic H. sapiens 62.5 4 x 6.0 ExpressMag Enhanced
OCM1 17828296 Skin Melanoma, uveal H. sapiens - - - -
OCM3 17828296 Skin Melanoma, uveal H. sapiens - - - -
MSTO-211H 18757427 Fibroblast Mesothelioma, malignant biphasic H. sapiens - - - -
BV-2 19232731 Nervous system Microglial M. musculus - - - -
CD34+ 17347684 Blood Monocyte H. sapiens - - - -
CD34+ 18202658 Blood Monocyte H. sapiens - - - -
Ba/F3 18068628 Blood, peripheral Murine (BALB/c) pro B M. musculus - - - -
Ba/F3 19079597 Blood, peripheral Murine (BALB/c) pro B M. musculus - - - -
NSC 18687996 Nervous system Neural Stem Cell M. musculus - - - -
IMR-32 17581253 Abdomen Neuroblastoma H. sapiens - - - -
IMR-32 18923523 Abdomen Neuroblastoma H. sapiens - - 1.0 Polybrene
SH-SY5Y 17581253 Bone Neuroblastoma H. sapiens - - - -
SK-N-BE 17102133 Bone Marrow Neuroblastoma H. sapiens - - - -
SK-N-AS 18558000 Epithelial Neuroblastoma H. sapiens - - 10.0 -
HSR-GMB 1 17628016 Nervous system Neuroblastoma H. sapiens - - - -
HTB-11 18768981 Nervous system Neuroblastoma H. sapiens - - - -
M1C 18672021 Nervous system Neuroblastoma H. sapiens - - - Polybrene
N18 18180767 Nervous system Neuroblastoma M. musculus - - - -
Neuro-2a 18941255 Nervous system Neuroblastoma M. musculus - - 1.0 -
Neuro-2a 19385050 Nervous system Neuroblastoma M. musculus - - - -
H1299 15731348 Lung Non-Small Cell Lung Cancer H. sapiens - - - -
H1299 18216111 Lung Non-Small Cell Lung Cancer H. sapiens - - - -
H1299 18337815 Lung Non-Small Cell Lung Cancer H. sapiens - - - -
H1299 18337823 Lung Non-Small Cell Lung Cancer H. sapiens - - - -
H1299 TRC-Broad Institute Lung Non-Small Cell Lung Cancer H. sapiens 12.5 0.5 µl/1000 cells 2.5 Utilize Spinfection
NCI-H1299 16982772 Lung Non-Small Cell Lung Cancer H. sapiens - - - -
NCI-H1299 18403640 Lung Non-Small Cell Lung Cancer H. sapiens - - - -
NCI-H1299 18948597 Lung Non-Small Cell Lung Cancer H. sapiens - - - -
U-2 OS 16887288 Bone Osteosarcoma H. sapiens - - - -
U-2 OS 16982772 Bone Osteosarcoma H. sapiens - - - -
U-2 OS 18426905 Bone Osteosarcoma H. sapiens - - - -
U-2 OS 18922873 Bone Osteosarcoma H. sapiens - - - -
U-2 OS 19036724 Bone Osteosarcoma H. sapiens - - - -
U-2 OS Sigma MISSION Team Bone Osteosarcoma H. sapiens 30.0 5 x 2.0 -
U-2 OS TRC-Broad Institute Bone Osteosarcoma H. sapiens 3.1 3.0 µl/1000 cells 1.5 Utilize Spinfection
U20 18403640 Bone Osteosarcoma H. sapiens - - - -
Hs840.T 17581253 Pharynx Papilloma H. sapiens - - - -
PC12 Sigma MISSION Team Adrenal Gland Pheochromocytoma R. norvegicus 7.8 20 x - >50% transduction without selection
H929 18431519 Bone Marrow Plasmacytoma, myeloma H. sapiens - - - -
MC3T3-E1 16728642 Bone Preosteoblast M. musculus - - - -
Primary HNSCC 18624948 - Primary - - - - -
B-Cells TRC-Broad Institute Blood Primary M. musculus 15.6 3.0 µl/1000 cells - -
Primary AML 19056693 Blood Primary H. sapiens - - - -
BMM Sigma MISSION Team Bone Marrow Primary M. musculus 468.8 8 x 3.0 ExpressMag Enhanced
Primary mouse bone marrow 18682599 Bone Marrow Primary M. musculus - - - -
TSC1 19052232 Bone Marrow Primary M. musculus - - - -
Primary kidney cells 18235101 Kidney primary M. musculus - - - -
Primary hepatocytes 17687072 Liver Primary M. musculus/R. norveticus - - - -
Astrocytes Sigma MISSION Team Nervous system Primary H. sapiens 50.0 1 x 2.0 -
Cortical Neuron TRC-Broad Institute Nervous system Primary M. musculus 31.3 0.4 µl/1000 cells 0.5 -
Cortical Neuron TRC-Broad Institute Nervous system Primary M. musculus 31.3 0.7 µl/1000 cells 0.5 Utilize Spinfection
Primary neurons 18604272 Nervous system Primary M. musculus - - - -
Primary β-cells 18599525 Pancreas Primary H. sapiens - - - -
Primary mouse melanocytes 17134690 Skin Primary M. musculus - - - -
82HTB 17210637 Muscle Rhabdomyosarcoma H. sapiens - - - -
RD 17581253 Muscle Rhabdomyosarcoma, embryonal H. sapiens - - - -
D17 Sigma MISSION Team Bone Sarcoma C. Lupus familliarus 20.8 5 x 2.0 -
GIST430 16954519 Gastrointestinal Sarcoma H. sapiens - - - -
GIST430 17452978 Gastrointestinal Sarcoma H. sapiens - - - -
GIST48 17452978 Gastrointestinal Sarcoma H. sapiens - - - -
GIST882 17452978 Gastrointestinal Sarcoma H. sapiens - - - -
hESCs 19620289 Embryo Stem Cell H. sapiens - - - -
ES 14688395 Embryo Stem Cells M. musculus - - - DNA Stable
V6.5 TRC-Broad Institute Embryo Stem Cells M. musculus 9.4 1.3 µl/1000 cells 3.5 Utilize Spinfection
V6.5mES 16153702 Embryo Stem Cells M. musculus - - - -
Primary NSCs 18948956 Nervous system Stem Cells, primary M. musculus - - - -
DAS 17418417 Aorta-gonad-mesonephros Stromal cells M. musculus - - - -
TG40 18411339 Blood T-Cell line M. musculus - - - -
immortalized B cells 19349959 Blood Tumor - - - - -
HEK293T 16954377 Kidney Tumor H. sapiens - - - -
HEK293T 17060456 Kidney Tumor H. sapiens - - - -
HEK293T 17277771 Kidney Tumor H. sapiens - - - -
HEK293T 17384642 Kidney Tumor H. sapiens - - - -
HEK293T 18948591 Kidney Tumor H. sapiens - - - -
MK24 16814714 - Tumor M. musculus - - 2.0 -
Sup-M2-TS 17371830 Blood Tumor H. sapiens - - - -
BT549 TRC-Broad Institute Breast Tumor H. sapiens 3.1 5.0 µl/1000 cells 2.0 -
MCF-7-I4 18353781 Breast Tumor H. sapiens - - - -
HEK293T 12930953 Kidney Tumor H. sapiens - - - DNA Transient
HEK293T 17386266 Kidney Tumor H. sapiens - - - DNA Transient
HEK293T 18573876 Kidney Tumor H. sapiens - - - DNA Transient
HEK293T Sigma MISSION Team Kidney Tumor H. sapiens 31.3 3 x 2.0 -
HEK293T Sigma MISSION Team Kidney Tumor H. sapiens 20.8 5 x 5.0 -
3Y1 17823279 Fibroblast Tumor R. norvegicus - - - -
NCI-H1437 TRC-Broad Institute Lung Tumor H. sapiens 3.8 2.5 µl/1000 cells 2.0 Utilize Spinfection
NCI-H1568 TRC-Broad Institute Lung Tumor H. sapiens 5.0 10.0 µl/1000 cells 2.0 Utilize Spinfection
NCI-H1792 TRC-Broad Institute Lung Tumor H. sapiens 1.9 3.3 µl/1000 cells 2.0 Utilize Spinfection
NCI-H1975 18948597 Lung Tumor H. sapiens - - - -
NCI-H1975 19079597 Lung Tumor H. sapiens - - - -
NCI-H1975 TRC-Broad Institute Lung Tumor H. sapiens 3.8 2.5 µl/1000 cells 2.0 Utilize Spinfection
NCI-H2009 TRC-Broad Institute Lung Tumor H. sapiens 2.5 2.5 µl/1000 cells 2.0 Utilize Spinfection
NCI-H23 18948595 Lung Tumor H. sapiens - - - -
NCI-H23 TRC-Broad Institute Lung Tumor H. sapiens 4.4 1.4 µl/1000 cells 2.0 Utilize Spinfection
NCI-H522 TRC-Broad Institute Lung Tumor H. sapiens 6.3 1.0 µl/1000 cells 2.0 Utilize Spinfection
1355T 16901783 Mammary Tumor - - - - -
SCp2 17535848 Mammary Gland Tumor H. sapiens - - - -
OV-30 17942676 Ovary Tumor H. sapiens - - - -
OV-90 17942676 Ovary Tumor H. sapiens - - - -
BJ-TERT 18922873 Skin Tumor, fibroblasts H. sapiens - - - -
BJ-TERT 18948591 Skin Tumor, fibroblasts H. sapiens - - - -
MON 18332116 - Tumor, rhabhoid malignant H. sapiens - - - -