不含EDTA的SigmaFAST™蛋白酶抑制剂混合物片剂

 最广谱的蛋白酶抑制


不含EDTA的SigmaFAST™蛋白酶抑制剂混合物片剂 (货号 S8830) 是便利的水溶性蛋白酶抑制剂混合物片剂,可抑制:
  • 丝氨酸蛋白酶
  • 半胱氨酸蛋白酶
  • 天冬氨酸蛋白酶
  • 金属蛋白酶
由于S8830不含EDTA,因此不会干扰下游应用,如纯化组氨酸标签蛋白。



在组织或细胞开始裂解时,提取物充满降解蛋白质的蛋白水解酶。在提取和纯化过程中保护蛋白免受酶促降解至关重要。具有功能性并与随后的下游应用相容的分离蛋白同样重要。该片剂对抑制丝氨酸、半胱氨酸、金属蛋白酶和天冬氨酸(酸性)蛋白酶具有广泛特异性,可用于一般目的或更专门的应用。该混合物以便利的水溶性片剂提供,可在不存在EDTA螯合剂或有机溶剂DMSO的情况下提供优异的保护。

 

 与镍树脂相容

Sigma-Aldrich的不含EDTA的片剂不会干扰HIS-tag蛋白纯化等下游应用。


通过在溶解的不含EDTA的片剂存在下反复从Sigma的HIS-Select镍树脂中回收HIS标签蛋白来进行测试。作为对照,在PBS(磷酸盐缓冲盐溶液)存在下回收相同的HIS标签蛋白。根据测量的蛋白浓度,两个样品组的重复(4次)回收率相同。

 

Compatible with Nickel Resin


 与竞品的比较

使用荧光蛋白酶抑制试验进行蛋白酶抑制。

Protease Inhibition using Fluorescent Protease Inhibition Assay


SDS-PAGE 分析显示酶消化具有优越的保护作用。

 SDS-PAGE analysis of enzyme digest showing superior protection

A. 对照,只含BSA
B. BSA, 胃蛋白酶
C. BSA, 胃蛋白酶、SIGMAFAST蛋白酶抑制剂混合物、不含EDTA的片剂
D. BSA, 胃蛋白酶、不含EDTA的竞品片剂

SDS-PAGE analysis of enzyme digest showing superior protection

A. 对照,只含BSA
B. BSA, 胰酶提取物
C. BSA, 胰酶提取物、SIGMAFASTä蛋白酶抑制剂混合物、不含EDTA的片剂


 相关产品

蛋白酶抑制剂混合物
蛋白酶抑制剂混合物
磷酸酶抑制剂混合物
HIS-Select 产品


 参考资料

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