FLAG 表达系统是表达、纯化和检测重组融合蛋白的成熟、可靠方法。

以下列出了许多与PubMed 关联的参考文献,您可从中查找到完整的摘要信息和相关的期刊信息。

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  Flag Literature

U2OS GFP-NUP98: 货号 CLL1136: U2OS 骨肉瘤敲入表达NUP98 基因(核孔蛋白98 kDa, NPC的外围组份),采用3xFLAG-GFP在N端内源性标记的细胞系。


标题 作者/期刊名称
Selected reaction monitoring mass spectrometry reveals the dynamics of signaling through the GRB2 adaptor. Bisson, N.,
Nat Biotechnol. 2011 Jun 26;29(7):
Identifying protein complexes by affinity purification and mass spectrometry analysis in the rice blast fungus. Liu, W.,
Methods Mol Biol. 2011;722:
Hepatitis C virus expressing flag-tagged envelope protein 2 has unaltered infectivity and density, is specifically neutralized by flag antibodies and can be purified by affinity chromatography. Prentoe, J.,
Virology. 2011 Jan 20;409(2):
Biochemical and cellular characterization of Helicobacter pylori RecA, a protein with high-level constitutive expression. Orillard E,
J Bacteriol. 2011 Dec;193(23):
Native capillary isoelectric focusing for the separation of protein complex isoforms and subcomplexes. Fonslow, BR.,
Anal Chem. 2010 Aug 1;82(15):
Herpes simplex virus proteins ICP27 and UL47 associate with polyadenylate-binding protein and control its subcellular distribution. Dobrikova E,
J Virol. 2010 Jan;84(1):
Cell-specific information processing in segregating populations of Eph receptor ephrin-expressing cells. Jørgensen, C.,
Science. 2009 Dec 11;326(5959):
Functional mapping of the interaction between TDP-43 and hnRNP A2 in vivo. D'Ambrogio, A.,
Nucleic Acids Res. 2009 Jul;37(12):
Severe acute respiratory syndrome coronavirus papain-like protease ubiquitin-like domain and catalytic domain regulate antagonism of IRF3 and NF-kappaB signaling. Frieman, M.,
J Virol. 2009 Jul;83(13):
Regulation of Set9-mediated H4K20 methylation by a PWWP domain protein. Wang, Y.,
Mol Cell. 2009 Feb 27;33(4):
Mdm2 regulates p53 mRNA translation through inhibitory interactions with ribosomal protein L26. Ofir-Rosenfeld, Y.,
Mol Cell. 2008 Oct 24;32(2):
The protein kinase C-responsive inhibitory domain of CARD11 functions in NF-kappaB activation to regulate the association of multiple signaling cofactors that differentially depend on Bcl10 and MALT1 for association. McCully, RR.,
Mol Cell Biol. 2008 Sep;28(18):
Functional regulation of MyD88-activated interferon regulatory factor 5 by K63-linked polyubiquitination. Balkhi, MY,
Mol Cell Biol. 2008 Dec;28(24):
High-throughput mapping of a dynamic signaling network in mammalian cells. Barrios-Rodiles, M.,
Science. 2005 Mar 11;307(5715):
Chibby cooperates with 14-3-3 to regulate beta-catenin subcellular distribution and signaling activity. Li, FQ., et al.
J.Cell Bio, 2008. 181(7):
Tandem affinity purification vectors for use in gram positive bacteria Yang, X., et al.
Plasmid, 2008. Jan; 59(1): 54-62.
Small-scale production of Burkolderia cepacia ATCC21808 lipase adapted to high-throughput screening Puech-Guenot, S., et al.
Journal of Biomolecular Screening, 2008. Jan; 13(1): 72-79.
Deficient RPS19 protein production induces cell cycle arrest in erythroid progenitor cells Kuramitsu, M., et al.
British Journal of Haematology, 2008. Feb; 140(3): 348-359.
Expression and purification of epitope-tagged multisubunit protein complexes from mammalian cells. Wu, S., et al.
Curr Protoc Mol Biol, 2002. Nov; Chapter 16: Unit 16.13.
Phosphorylation of two eukaryotic transcription factors, Jun dimerization protein 2 and activation transcription factor 2, in Escherichia coli by Jun N-terminal kinase 1. Murata, T., et al.
Analytical Biochemistry, 2008. Feb 6; 376(1): 115-121.
Proteomic identification of the cerebral cavernous malformation signaling complex Hilder, T. L., et al.
J. Proteome. Res., 2007. Nov; 6(11): 4343-55.
Reversible interactions between smooth domains of the endoplasmic reticulum and mitochondria are regulated by physiological cytosolic Ca2+ levels Goetz, J. G., et al.
J. Cell Sci., 2007. Oct 15; 120(Pt 20): 3553-64.
A homogeneous fluorometric assay platform based on novel synthetic proteins Vardar-Schara, G., et al.
Biochem. Biophys. Res. Commun., 2007. Sept 14; 361(1): 103-8.
Functional expression of human pyruvate carboxylase for reduced lactic acid formation of Chinese hamster ovary cells (DG44) Kim, S. H., et al.
Appl. Microbiol. Biotechnol., 2007. Sept; 76(3): 659-65.
Generic method for quantification of FLAG-tagged fusion proteins by a real time biosensor. Mersich, C., et al.
J. Biochem. Biophys. Methods, 2007. Jun 10; 70(4): 555-63.
Expression and purification of recombinant human Angiopoietin-1 produced in Chinese hamster ovary cells Hwang, S. J., et al.
In Vitro Cell. Dev. Biol. - Anim., 2007. May-Jun; 43(5-6): 162-7.
Interaction between Cell Division Proteins FtsE and FtsZ. Corbin, B. D., et al.
J. Bacteriol., 2007. Apr; 189(8): 3026-35.
SipA, SopA, SopB, SopD and SopE2 effector proteins of Salmonella enterica serovar Typhimurium are synthesized at late stages of infection in mice Giacomodonato, M. N., et al.
Microbiology, 2007. Apr; 153(Pt 4): 1221-8.
Regioselective labeling of antibodies through N-terminal transamination. Scheck, R. A., et al.
ACS Chemical Biol., 2007. April; 2(4): 247-251.
Tyrosine residues 654 and 670 in beta-catenin are crucial in regulation of Met-beta-catenin interactions. Zeng, G., et al.
Exp Cell Res, 2006. Nov 1; 312(18): 3620-30.
Structure of anti-FLAG M2 Fab domain and its use in the stabilization of engineered membrane proteins. Roosild, T. P., et al.
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun., 2006. Sept 1; 62(9): 835-9.
Design and Characterization of Stimuli-Responsive FLAG-tag Analogues and the Illumination-Induced Modulation of Their Interaction with Antibody 4E11. Pöehner, C., et al.
Biotechnol. Prog., 2006. July-Aug; 22(4): 1170-8.
Construction of eukaryotic expression vector for HPC2 and its expression in HEK293 cells. Tan, K. L., et al.
Di Yi Jun Yi Da Xue Xue Bao, 2005. Dec; 25(12): 1482-1484, 1497.



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