异丙醇分子生物学试剂

产品编号:I9516

CAS RN 67-63-0
EC 200-661-7
别名:2-丙醇,仲丙醇,IPA,异丙醇


 产品描述

异丙醇是一种极性有机溶剂,广泛应用于化学、生物化学和分子生物学。工业应用包括其在防冻剂、速干油和快干油墨中的应用,另外还用作树胶、虫胶和精油的溶剂1。异丙醇已被用作合成固相酰化剂的溶剂2

异丙醇用于沉淀哺乳动物组织的DNA,从DNA样品中提取溴化乙锭,以及从细胞和组织中分离RNA等的实验方案已有报道3。异丙醇已用于蛋白质结晶4,5,6。已有报道称,使用异丙醇从生物基质中分离出类视黄醇用于HPLC和HPLC-MS分析7,8。据报道,已对异丙醇和其他有机溶剂进行了数学分析,检测了反相HPLC系统中固定相和有机改性剂的选择性9


 注意事项及免责声明

本产品仅供研发使用,不适用于药物、家庭或其他用途。有关有害物及其安全处理方法的信息,请参阅安全数据表


 制备说明

该产品可与水、醇、醚和氯仿混溶1


 产品简介

分子式:C3H8O
分子量:60.10
沸点:82.5 °C (760 torr)1
密度:0.78505 g/ml (20 °C)


 特异性

该产品被指定为分子生物学级产品,适用于核酸的沉淀。


 材料

     


 参考文献

  1. The Merck Index, 12th ed., Entry# 5227.
  2. Nicewonger, R. B., et al., Synthesis of a novel, recyclable, solid-phase acylating reagent. Bioorg. Med. Chem. Lett., 12(14), 1799-1802 (2002).
  3. Molecular Cloning: A Laboratory Manual, 3rd ed., Sambrook, J. and Russell, D.W., CSHL Press (Cold Spring Harbor, NY: 2001), pp. 1.151, 6.23- 6.30, 7.4-7.12, A8.27.
  4. Romao, M. J., et al., Subunit composition, crystallization and preliminary crystallographic studies of the Desulfovibrio gigas aldehyde oxidoreductase containing molybdenum and [2Fe-2S] centers. Eur. J. Biochem., 215(3), 729- 732 (1993).
  5. Knapp, S., et al., Crystallization and preliminary crystallographic analysis of an amylopullulanase from the hyperthermophilic archaeon Pyrococcus woesei. Proteins, 23(4), 595-597 (1995).
  6. Yang, F., et al., Crystal structure of cyanovirin-N, a potent HIV-inactivating protein, shows unexpected domain swapping. J. Mol. Biol., 288(3), 403-412 (1999).
  7. Schmidt, C. K., et al., Chromatographic analysis of endogenous retinoids in tissues and serum. Anal. Biochem., 315(1), 36-48 (2003).
  8. McCaffery, P., et al., Retinoid quantification by HPLC/MSn. J. Lipid Res., 43(7), 1143-1149 (2002).
  9. Du, C. M., et al., Characterizing the selectivity of stationary phases and organic modifiers in reversed-phase high-performance liquid chromatographic systems by a general salvation equation using gradient elution. J. Chromatogr. Sci., 38(11), 503-511 (2000).