Ascentis® Express U/HPLC Columns

A Breakthrough in HPLC Column Performance

Based on Fused-Core® Particle Technology, Ascentis Express HPLC columns offer significant performance benefits over popular columns based on totally porous particles.

Ascentis Express UHPLC columns based on 2.0 µm particles provide an optimized solution for high throughput small molecule analysis.

Ascentis Express columns based on 2.7 μm particles provide the high efficiency separation of sub-2-μm particles, but at higher speed and half of the backpressure. This lower pressure means that Ascentis Express can be run on all HPLC systems including conventional as well as UHPLC systems.

Ascentis Express columns based on 5 μm particles provide faster, more efficient separations compared to popular 3 μm and 5 μm totally porous particles, making them a good choice for improving the performance of traditional HPLC systems.

Ascentis Express Hyper-Fast Separations
High-Definition Resolution
Technical Resource GuideTechnical Resources Fast HPLC Application SupportApplications Support

Ascentis Express Products

Product Selection Guide

Find the right Ascentis Express product to best suit your needs.

C18 HPLC Columns

2.0 µm | 2.7 µm | 5 µm
The first choice for starting a new method.

2.7 µm
Separate compounds that are challenging to resolve or elute early on C18 or other phenyl phases

RP-Amide HPLC Columns

2.0 µm | 2.7 µm | 5 µm
Combining an embedded polar group stationary phase and Fused-Core particles.

Phenyi-Hexyl HPLC Columns

2.0 µm | 2.7 µm | 5 µm
Combining the popular phenyi-hexyl stationary phase and Fused-Core particles.

C8 HPLC Columns

2.0 µm | 2.7 µm | 5 µm
Ideal for non-polar compounds that are reatined too strongly by C18.

ES-Cyano HPLC Columns

2.0 µm | 2.7 µm | 5 µm
Provides stable, reversed-phase packing for basic, acidic or neutral compounds.


2.0 µm | 2.7 µm | 5 µm
Ideal for many classes of polar compounds.

F5 HPLC Columns

2.0 µm | 2.7 µm | 5 µm
Separate closely related compounds.

OH5 HPLC Columns

2.0 µm | 2.7 µm | 5 µm
Pentahydroxy phase for fast analysis of polar compounds.

Peptide ES-C18 HPLC Columns

2.7 µm
Separate high molecular weight compounds such as peptides and small proteins.


Featured Articles

 Reporter EU Volume 30

Fast HPLC Using Fused-Core Particle Technology for Rapid Screening of Pharmaceutical Compounds

HPLC is critical to the discovery, development and eventual commercialization of pharmaceutical products. HPLC is the benchmark analytical method in the pharmaceutical industry due to its…

Read More

 Reporter US Volume 29.5

Serum Lipidomics Profiling using an Ascentis Express Fused-Core C18 Column

Chromatographic separation of lipid extracts prior to mass spectrometric (MS) detection aids in the achievement of comprehensive, quantitative, and reproducible analytical data, in an efficient and robust manner…

Read More

 Reporter US volume 28.1

Accelerated Bioanalytical LC-MS-MS Using Ascentis Express Fused-Core HPLC Columns

Fast bioanalysis requires the use of short columns packed with high efficiency particles. The use of HPLC columns packed with Fused-Core particles and sub-2 μm porous particles have led the way…

Read More

Ascentis Express Training

Nick the Hero

Nick the Hero

Learn how Nick boosts HPLC productivity.
Introduction to Ascentis Express

Intro to Ascentis Express

A 9-minute presentation on Ascentis Express columns.
HPLC Webcasts

HPLC Webcasts

Live and recorded webcasts on
our BrightTALK channel.
Ascentis Express Demo

Ascentis Express Demo

A 28-minute presentation on Ascentis Express columns.

View more HPLC videos & webcasts


Fused-Core is a registered trademark of Advanced Materials Technology, Inc.