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Discovery BIO PolyMA-SCX
Polymethacrylate polymer-based strong cation-exchange HPLC column
Discovery BIO PolyMA-SCX polymer-based cation-exchange particles have discriminating hydrophilic surface chemistry making them ideally suited for separating proteins, peptides, and other biotechnology-derived products.

Choose Discovery BIO PolyMA-SCX for cation-exchange separations. Generally run at a pH less than the protein's pI (isoelectric point) - usually below pH 7.
Particle:   Polymethacrylate with hydrophilic coating
Functional Group:   Sulfopropyl (SP)
Counterion (as supplied):   Na+
Particle Shape:   Spherical, monodispersed
Particle Size:   5µm
Pore Size:   1000Å
Coverage:   0.3meq/g
pH Range:   1 to 13
Temperature Range:   4°C to 50°C
Solutions for Protein and Peptide Separation Challenges

Excellent separations of protein isoforms

High resolution at low sample load

Quantitative recovery - a hydrophilic surface eliminates protein adsorption

High efficiency

Wide pH range

Here's the
Discovery BIO PolyMA-SCX typically gives better resolution than competitive polymeric ion-exchange materials.
Comparison of Efficiency: Cytochrome c Variants
Columns:   Discovery BIO PolyMA-SCX, 5cm x 4.6mm, 5µm (Cat. No. 59601-U) and Competitive polymeric-SCX columns of similar dimensions
Mobile Phase:   (A) 50mM MOPS/KOH, pH 7.0;
(B) 50mM MOPS/KOH, 0.5M KCl, pH 7.0
Flow:   3.01cm/min (flow rates appear in Figure)
Temp.:   35°C
Detection:   UV, 280nm
Sample:   10µg each variant
Gradient Profile:   0.6% B per minute. See Figure for details.
1.   Cow cytochrome c
2.   Horse cytochrome c
3.   Rabbit cytochrome c
Although capacity is dependent on the protein under study and operating conditions, generally between 20 and 50mg of total protein can be loaded onto the Discovery BIO PolyMA-SCX columns.
Ion-exchange Capacity
Column Protein mg per mL of column volume mg per column
Discovery BIO PolyMA-SCX Lysozyme 40mg 30mg
Loading studies were run at 240cm/hr (0.67mL/min) using 0-1M NaCl salt gradients in 20mM sodium phosphate, pH 7. Protein concentration was 2mg/mL in starting mobile phase.
Protein (Fibrinogen) Recovery  
  Cytochrome C Recovery
Column 1st Injection 2nd Injection 5th Injection
Discovery BIO PolyMA-SCX (5µm, 5cm x 4.6mm) 99.5% 98.6% 100.2%
Competitive polymethacrylic SP column (10µm, 7.5cm x 7.5mm) 59.0% 76.1% 90.6%
Mobile phase: Gradient of 0 - 1M NaCl in 20mM citrate (pH 4) over 30 minutes. Flow Rate: 1mL/min. Ambient temperature. Sample: 40µg cytochrome c in 20µL starting mobile phase.

Column stability is important from quality and economic standpoints. Stable columns give reliable results. Stable columns cost less per injection and cause less system down-time. Results obtained on Discovery BIO PolyMA-SCX will be reproducible injection after injection because of exceptional column stability. In the figure below, Discovery BIO PolyMA-SCX columns are shown to give stable retention of three proteins after 500 injections, with no sign of deterioration.
Stability of Discovery BIO PolyMA-SCX Columns
Column:   Discovery BIO PolyMA-SCX, 5cm x 4.6mm, 5µm (Cat. No. 59601-U)
Mobile Phase:   (A) 20mM sodium phosphate, pH 7.0
(B) 20mM sodium phosphate, 0.5M NaCl, pH 7.0
Flow:   0.5mL/min
Temp.:   25°C
Detection:   UV, 280nm
Sample:   Ribonuclease A (10µg), a-chymotrypsinogen (5µg), cytochrome c (5µg)
Gradient Profile:  
time (min.) %A %B
0 95 5
15 0 100


Ordering Information
Discovery BIO PolyMA-SCX HPLC Column
Particle Size (µm) ID (mm) Length (cm) Cat. No.
5 4.6 5 59601-U
Column Protection: We recommend filtering samples and using an in-line filter to protect Discovery BIO PolyMA-SCX columns.
Description Cat. No.
In-line filter, 0.5 micron frits Z227323

view the pdf documentTo view the Care and Use Guide for Discovery BIO PolyMA-SCX (10KB PDF), click the pdf icon.

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