We are currently upgrading the certain systems that support this website.  You may experience some functionality loss, though we expect the site to be fully functional by 9:00PM CT (US), February 24th.

We apologize for any inconvenience and appreciate your patience during this improvement process.Please note that you still have telephone and email access to our local offices. We apologize for any inconvenience.


HybridSPE-PPT “In-Well” 96-well Precipitation Visual

1. Precipitate Proteins by adding 100 µL plasma or serum to the HybridSPE-PPT plate followed by 300 µL 1% formic acid in acetonitrile. Add I.S. as necessary.

2. Mix by vortexing/shaking HybridSPE-PPT plate or by aspirating/ dispensing with 0.5-1 mL pipette tip (e.g., TOMTEC Quadra liquid handler)


Apply vacuum.
The packed-bed filter/frit assembly acts as a depth filter for the concurrent physical removal of precipitated proteins and chemical removal phospholipids. Small molecules (e.g., pharma compounds and metabolites) pass through unretained.

4. Resulting filtrate/eluate is free of proteins and phospholipids and ready for immediate LC-MS-MS analysis; or it can be evaporated and reconstituted as necessary prior to analysis