Cell Culture

Hepatocyte Culture

Sigma is proud to offer a selection of media, many of which are serum-free, for the in vitro culture of human primary cells, such as keratinocytes, endothelial cells, and epithelial hepatocytes. These primary cell types have potential commercial value in a wide range of emerging technologies and therapeutic applications. Among these are: tissue engineering applications, such as wound healing and burn treatment; high throughput screening for drug toxicology and efficacy studies; gene therapy; pseudo-organs (e.g.. artificial liver) and as model cell systems for developing new therapeutic approaches to human diseases.

 


 

 Hepatocyte Cell Culture System

Hepatocytes are epithelial cells found in the liver. They perform important functions, such as helping to detoxify blood, and to synthesize transport proteins, such as lipoprotein, albumin and transferrin.  Primary and secondary cultures of hepatocytes are useful for studying the mechanisms of liver regeneration and differentiation.  Historically, primary hepatocytes have exhibited a limited replicating lifespan in culture.  In addition, when stimulated to divide in culture they have generally lost differentiated functions such as the ability to synthesize and secrete albumin and transferrin.  While substantial progress has made in understanding the factors which affect these characteristics of hepatocytes in culture, primary and secondary hepatocytes are still not suitable for use in some emerging medical technologies and applications.  Among the emerging potential applications for cultured hepatocytes are gene therapy, bio-artificial organs, cell transplants, drug production, and drug and chemical testing.  These technologies may benefit from the use of cloned and immortalized hepatocytes.  Hence, there is a significant effort to develop hepatocyte cell lines for this purpose.

Primary Human Hepatocyte Culture System

We have partnered with industry leaders, BioreclamationIVT and LifeNet Health, to provide high-quality primary human hepatocytes products . In order to best meet the specific needs of a wide variety of study types, we offer different hepatocyte products derived from human liver tissue:

BioreclamtionIVT:

  • LiverPool™ Cryoplateable Human Hepatocytes
  • LiverPool™ Human Hepatocytes
  • Cryopreserved Human Hepatocytes
  • Cryoplatable and Cryopreserved Human Hepatocytes

LifeNet Health:

  • Single Donor Cryoplateable Human Hepatocytes
  • Single Donor Cryopreserved Human Hepatocytes

 

Product # Product Name
MTOXH1000 Cryoplateable Primary Human Hepatocytes
MTOXH1002 Cryoplateable Primary Human Hepatocytes Induction Certified
MTOXH1008 Cryopreserved Primary Human Hepatocytes High BMI
MTOXH1012 Cryopreserved Primary Human Hepatocytes Juvenile
MTOXH1001 Cryopreserved Primary Human Hepatocytes Metabolism Qualified Suspension Grade
MTOXH1011 Cryopreserved Primary Human Hepatocytes Neonatal
MTOXH1005 Cryopreserved Primary Human Hepatocytes Research Grade
F00995 Female Human Cryopreserved Hepatocytes 1 ea of ( 5 million cells per vial)
FX008001 Female Human LiverPool™ 10-donor pooled Cryopreserved Hepatocytes 1 ea of ( 5 million cells per vial)
F00995-P F00995-P Female Human Plateable Cryopreserved Hepatocytes 1 ea of ( 5 million cells per vial)
M00995 Male Human Cryopreserved Hepatocytes 1 ea of ( 5 million cells per vial)
MX008001 Male Human LiverPool™ 10-donor pooled Cryopreserved Hepatocytes 1 ea of ( 5 million cells per vial)
X008052-P Mixed Gender Human LiverPool™ 5-donor pooled Cryoplateable Hepatocytes 1 ea of ( 5 million cells per vial)
X008001-P Mixed Gender Human LiverPool™ 10-donor pooled Cryoplateable Hepatocytes
X008052 Mixed Gender Human LiverPool™ 5-donor pooled Cryopreserved Hepatocytes
X008001 Mixed Gender Human LiverPool™ 10-donor pooled Cryopreserved Hepatocytes 1 ea of ( 5 million cells per vial)
X008000 Mixed Gender Human LiverPool™ 20-donor pooled Cryopreserved Hepatocytes 1 ea of ( 5 million cells per vial)
X008005 Mixed Gender Human LiverPool™ 50-donor pooled Cryopreserved Hepatocytes 1 ea of ( 5 million cells per vial)

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Primary Human Hepatocyte Culture Medium
 

Product # Product Name
MED-HHCM Human Hepatocyte Culture Medium 
MED-HHCMSP Human Hepatocyte Culture Medium Supplement Pack 
MED-HHPM Human Hepatocyte Plating Medium 
MED-HHPMSP Human Hepatocyte Plating Medium Supplement Pack 
MED-HHTM Human Hepatocyte Thawing Medium 
Z990004 InVitroGRO CP Medium
Z99029 InVitroGRO CP Medium
Z990003 InVitroGRO CP Medium
Z990002 InVitroGRO HI Medium
Z99009 InVitroGRO HI Medium
Z990012 InVitroGRO HI Medium
Z990006 InVitroGRO HT Medium
Z99019 InVitroGRO HT Medium
Z990005 InVitroGRO HT Medium
Z99074 InVitroGRO KHB Medium
Z990001 InVitroGRO PT Medium
Z99000 Torpedo Antibiotic Mix
Z990007 Torpedo Antibiotic Mix
Z990008 Torpedo Antibiotic Mix

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 Hepatocyte Cell Culture Medium

Many classical media (see web-pages in our "Classical Media" section) have been used to culture epithelial hepatocytes. Some examples of these are; Liebovitz L-15, DMEM/F-12, RPMI 1640, Waymouth's MB 752/1 and Williams Medium E.  Growth of hepatocytes in these media requires the use of of serum.

Our most advanced formulation for culturing primary, secondary, and cloned immortalized epithelial hepatocytes is H 1777, which is an enriched variation of Leibovitz L-15 formulation.  It may be successfully used with reduced concentrations of FBS.

 

Product # Product Name Description Application
H 1777 Hepatocyte Medium
  • Liquid,
  • Proprietary Mixture;
  • With HEPES and sodium bicarbonate buffers;
  • Without L-glutamine;
  • Sterile filtered;
  • Endotoxin tested;
  • Cell culture tested.

H1777 is an enhanced medium formulation based on the original Liebovitz, L-15 medium. It is formulated to support the  proliferation of primary, secondary and immortalized hepatocytes.

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 Hepatocyte Cell Culture Companion Products and Supplements

The culture of epithelial hepatocyte cells is supported by a series of products offered by Sigma.  The table below provides a partial list of Sigma products recommended for this purpose.  Also, see the "Hepatocyte Cell Culture Media" table above.

 

Product # Product Name Description Application
C 6295 Cell Freezing Medium-DMSO, serum-free
  • Liquid
  • Contains minimum essential medium (MEM), dimethyl sulfoxide (8.7%) and methycellulose
  • Sterile filtered
  • Endotoxin tested
  • Cell culture tested

Complete ready to use medium for the cryopreservation of cells.

C 2799 Collagenase
  • Powder
  • High Purity, Type VII
  • Activity: 1000-3000 collagen digesting units/mg solid; 4-12 FALGPA hydrolysis units/mg solid.
  • Contaminating activity: < 1 unit neutral protease and < 1 unit clostripain/mg solid
  • Cell culture tested

Useful for the isolation of epithelial hepatocytes when used in liver perfusion solutions.

D 1435 Dimethyl Sulfoxide, USP
  • Liquid
  • USP tested

DMSO has been shown to stimulate the redifferentiation of dedifferentiated epithelial hepatocytes in vitro.

H 0887 HEPES Buffer, (1M)
  • Liquid
  • Prepared from HEPES free acid and tissue culture grade water
  • Sterile filtered
  • Endotoxin tested
  • Cell culture tested

Useful for the isolation of epithelial hepatocytes when used in liver perfusion solutions.

H 3784 HEPES, sodium salt
  • Powder
  • Purity: > 99.5%, DNase, RNase and protease non detected, < 5 ppm heavy metals and < 5 ppm iron
  • pKa = 7.31 at 37°C; useful pH range 6.8-8.2
  • Endotoxin and bioburden tested
  • Cell culture tested

Useful for the isolation of epithelial hepatocytes when used in liver perfusion solutions.

H 3884 Hyaluronidase
  • Powder, lyophilized
  • Source: Bovine testes
  • High Purity, Type IV-S
  • M. Wt. = 56 kDa
  • Randomly cleaves glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates
  • Activity: 750-1,500 units/mg solid.  Assayed per the USP XXIV-NF XVII combined edition.
  • Contaminating activity: < 1 unit neutral protease and < 1 unit clostripain/mg solid
  • Cell culture tested

Useful for the isolation of epithelial hepatocytes when used in liver perfusion solutions.

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