Biological Buffers

TAPS

Synonym: N-[Tris(hydroxymethyl)methyl]-3-aminopropanesulfonic acid, [(2-Hydroxy-1,1-bis(hydroxymethyl)ethyl)amino]-1-propanesulfonic acid
 

TAPS is a zwitterionic buffer used in biochemistry and molecular biology. TAPS is utilized in capillary electrophoresis of such materials as DNA and DNA-dye complexes1,2,3,4. Dyes have been separated by planar chromatography with electroosmotic flow with 1 mM TAPS in the mobile phase5. The stability constants of metal ions with TAPS have been investigated by capillary electrophoresis6.
Useful pH Range 7.7 - 9.1
Working concentration range 20mM-150mM10,11

 

        Comparison of products by grade or quality level
Name CAS Molecular Formula Mol Wt Reagent Grades Life Science Grades PharmaGrade  Solutions
TAPS 29915-38-6 C7H17NO6S 243.28 T5130 394675 T5316 T9659    
TAPS sodium salt 91000-53-2 C7H16NNaO6S 265.26 T0647 T5441    

 

Interaction Comments
 

TAPS has been shown to inhibit connexin channel activity7.
TAPS can serve as a stabilizer for the globular protein BSA, in aqueous solutions8
Strong interaction with Cr, Fe and Cu. Slight interaction with Zn, Cd and Pb9

 

Literature References

  1. Zeng, Z., et al., Improved stability and electrophoretic properties of preformed fluorescent cationic dye-DNA complexes in a TAPS-tetrapentylammonium buffer in agarose slab gels. Anal. Biochem., 252(1), 110-114 (1997).
  2. Li, L., and McGown, L. B., Comparison of sieving matrices for on-the-fly fluorescence lifetime detection of dye-labeled DNA fragments. Fresenius J. Anal. Chem., 369(3-4), 267-272 (2001).
  3. Wei, W., and Yeung, E. S., Improvements in DNA sequencing by capillary electrophoresis at elevated temperature using poly(ethylene oxide) as a sieving matrix. J. Chromatogr. B Biomed. Sci. Appl., 745(1), 221-230 (2000).
  4. Liang, D., et al., Effect of glycerol-induced DNA conformational change on the separation of DNA fragments by capillary electrophoresis. J. Chromatogr. A, 931(1-2), 163-173 (2001).
  5. Nurok, D., et al., Separation using planar chromatography with electroosmotic flow. J. Chromatogr. A, 903(1-2), 211-217 (2000).
  6. Muzikar, M., et al., Capillary electrophoretic study of interactions of metal ions with crown ethers, a sulfated β-cyclodextrin, and zwitterionic buffers present as additives in the background electrolyte. Electrophoresis, 23(12), 1796-1802 (2002).
  7. Bevans, C. G, and Harris, A. L., Regulation of connexin channels by pH. Direct action of the protonated form of taurine and other aminosulfonates. J. Biol. Chem., 274(6), 3711-3719 (1999).
  8. Gupta, Bhupender S.; Taha, Mohamed; Lee, Ming-Jer. 2013. Process Biochemistry. 48.11: 1686-1696.
  9. Ferreira CM, Pinto IS, Soares, EV, Soares HM. 2015 Royal Society of Chemistry 30989- 31003
  10. Chankvetadze B –Capillary Electrophoresis in Chiral Analysis—John Wiley &Sons, 1997 pp.175
  11. Chegwidden WR, Carter ND, Edwards YH –The Carbonic Anhydrases New Horizons—Springer Basel AG, 2000 pp.526