Custom DNA Oligos


Sigma is pleased to offer OligoEvaluator, our online oligonucleotide sequence calculator.


Modules Features

Enter up to 10 sequences at a time, and the tool returns values for all major physical properties, such as molecular weight, melting temperature, secondary structure, and primer dimer formation (secondary structure and primer dimer formation information provided in simple-to-interpret text format, e.g. secondary structure--strong)


Enter the quantity, desired concentration, and molecular weight, and the tool returns the volume of water or buffer needed to resuspend a dry oligonucleotide

Dilution Enter the stock (resuspension) concentration, desired final volume, and desired final concentration, and the tool returns the volume of stock solution needed for dilution

Advanced Features

Feature Description
Reaction Conditions

Review secondary structure analysis and primer dimer formation to check for problematic folding

BLAST Search Check for oligonucleotide sequence homology

OligoEvaluator is easy to use: register or login, select DNA or RNA, paste your sequence, and click calculate. All reported properties are available for export to a convenient Excel template.

Your OligoArchitect™ Online login can be used for OligoEvaluator.