Measure cellular response quantitatively – with single-cell precision
ELISpot assays are used in a range of applications to monitor immunological response patterns, and their utility for the identification of antigenic peptides has proven useful for the development of immunotherapeutics and vaccine strategies. Validated ELISpot assays are highly reliable, reproducible and predictive. They can successfully identify antigen-specific T or B cells at the single cell level with results that closely simulate in vivo conditions, and can eliminate the need for long-term in vitro cell cultures.
ELISpot combines plate-based Enzyme Linked Immunosorbent Assays (ELISAs) with membrane-based Western blotting technologies for assessing immune cell function based on secreted analytes. T and B lymphocyte activation and related responses are key to clinical outcome in cancer and in a variety of other human diseases, but simultaneous measurement of key secreted factors can present a technical challenge. The ELISpot technique enables multi-target, quantitative assessment of effector functions at the single cell level, with exceptional sensitivity and resolution.
Membrane characteristics and application validation are key for this precise immunodetection technique. We are proud to be the leading supplier of validated ELISpot assays that incorporate unsurpassed Millipore membranes to the life science and biotherapeutic development communities.
The ELISpot Assay Workflow

- Coat membrane with capture antibody. Add immune cells and stimuli.
- Responding cells produce cytokines. The cytokine of interest binds to the capture Ab.
- Wash away cells. Add a second cytokine-specific biotinylated Ab, which binds the cytokine-Ab complex.
- Add enzyme substrate and develop. Each spot within a well represents the cytokine signature of a single cell.
The ELISpot technique can be used to detect diverse cellular responses that include
- cytolytic activity
- secretion of multiple cytokines and other bioactive molecules
- proliferation
Ordering Information
MultiScreen® HTS 96-well Plates*
Description |
Plate material/
color |
Qty/Pk |
Sterile |
Cat. No. |
MultiScreen® HTS-HA plates with MCE membrane |
Styrene/clear
|
10 |
Yes |
MSHAS4510 |
MultiScreen® HTS-IP plates with Immobilon®-P membrane |
Acrylic/clear
Acrylic/clear
Acrylic/white
Acrylic/white |
10
50
10
50
|
Yes
No
Yes No
|
MSIPS4510
MSIPN4550
MSIPS4W10 MSIPN4W50
|
MultiScreen® plates with Immobilon®-P membrane without underdrain |
Acrylic/white No underdrain |
10 |
Yes |
MAIPSWU10 |
MultiScreen® HTS plates with Immobilon®-P-FL membrane, with underdrain |
Acrylic/white |
10 |
Yes |
S5EJ104I07 |
* Classic MultiScreen® plates are also available for ELISpot assays. Contact technical service for information on catalogue numbers MAHAS4510, MAIPS4510 and S2EM004M99.
8-well strip
Description |
Plate material/
color |
Qty/Pk |
Sterile |
Cat. No. |
MultiScreen® 8-well strip with Immobilon®-P membrane |
Acrylic/Clear |
10 |
Yes |
M8IPS4510 |
Accessories
Description |
Qty/Pk |
Cat. No. |
Plate sealing tape, opaque |
100 |
MATAH0P00 |
MultiScreen® 8-Well Strip Support Frame |
10 |
M8IPFRAME |