|
|
| Large-Scale and High Titer Lentiviral Particles |
| Sigma has a versatile manufacturing platform for high-throughput or large-scale viral production of most HIV-1-based lentiviral vectors. The standard format of the MISSION TRC shRNA collection is 200 µl of 1 x 106 TU/ml (via p24 assay) minimum titer*. Any clone or control can be produced at larger volumes with the same minimum titer specification or higher titers. We also accommodate large-scale and high titer viral preparation using clones you send to us (must be compatible with our system). Sigma’s robust and optimized processes will save you time and reduce variability in your transduction experiments. Please see the table below for examples of our standard scale-up options. To review pricing or to submit a request for a quotation on your specific project, please use our MISSION Custom Request Submission Form. |
| Titer |
Volume |
Packaging |
Min. # Particles |
| 1 x 106 TU/ml* |
1 ml |
5 x 200 µl |
1 x 106 |
| 1 x 106 TU/ml* |
5 ml |
25 x 200 µl |
5 x 106 |
| 1 x 108 TU/ml |
0.2 ml |
4 x 50 µl |
20 x 106 |
| 1 x 108 TU/ml |
1 ml |
10 x 100 µl |
100 x 106 |
| 1 x 108 TU/ml |
2 ml |
20 x 100 µl |
200 x 106 |
| 1 x 109 TU/ml |
0.2 ml |
4 x 50 µl |
200 x 106 |
| 1 x 109 TU/ml |
1 ml |
10 x 100 µl |
1000 x 106 |
| Other scales and packaging configurations are accommodated. |
| *1.0 x 106 TU/ml via p24 assay is guaranteed. Our average titer is >1.0 x 107 TU/ml via p24 assay. |
| back to top |
| Send Your Clones for Lentiviral Manufacturing |
| Our versatile manufacturing platform for high-throughput or large-scale viral production can be used for most HIV-1-based lentiviral vectors. Investigators are not limited to clones that we currently supply. Alternatively, clones developed in private laboratories can be sent to Sigma for viral production within our quality system. For example, we have provided a custom EGFR SH2 binding domain vector in lentivirus for preparation of an EGFR Biosensor cell line. The size of the target sequence, gene sequences, as well as promoters can affect lentiviral titers during production. We will work to optimize viral production if necessary to exceed our minimum titer specifications. Submit a request for a quotation on your specific project by using our MISSION Custom Request Submission Form. |
| back to top |
| High-throughput DNA and Lentiviral Manufacturing |
| Sigma-Aldrich has in-house developed technologies that generate the highest quality DNA for high-throughput DNA manufacturing. The quality and yields of our DNA are ideal for processing of shRNA clone libraries and result in the highest quality lentiviral particles available. Our titers of lentiviral particles generated in a high-throughput fashion are routinely in the range of 1 to 4 x 107 TU/ml (via p24 assay), well beyond our minimum titer specification. Requests for additional information may be routed through your local biotech sales representative or by emailing MISSIONRNAi@sial.com. |
back to top
| Custom Gene Family Sets |
| A large number of gene families have already been assembled and are available from Sigma in bacterial glycerol stock and lentiviral formats. For more information on these sets, please visit the Gene Family Sets page. Please inquire if you are interested in DNA formats or if you would like to have us assemble a specialized gene set that is not currently available. Requests may be routed through your local biotech sales representative or by emailing MISSIONRNAi@sial.com. |
| QC Assays and Specifications |
| We will work with you to modify the number and types of tests performed. Please inquire with your project details. Email us at MISSIONRNAi@sial.com |
| Packaging Materials |
| Options such as plate style can be modified. Please inquire with your project details. Email us at MISSIONRNAi@sial.com |
| Contact Us |
| For questions about the library, pricing and quotes or other concerns, please email us at: MISSIONRNAi@sial.com. |
|
|
