Webinar: Need to validate your genome editing experiment? LentiORF to the rescue!

 

 What Does it Cover?

Whether you are looking to perform gene overexpression studies or validate gene knockdown/knockout results from your RNAi/CRISPR experiments, LentiORFs are your ideal shortcut to protein expression and gene analysis. These genes are available in either pooled or arrayed libraries, and can also be combined into gene family sets and custom clone panels to meet diverse research needs. They are excellent reagents for gain-of-function screens and are a powerful complement to CRISPR and RNAi. In this webinar, we will introduce the LentiORF library and its various formats. We will discuss the application of this technology as it pertains to experimental design, delivery mechanisms, data analysis and target validation. Further, we will present recent data demonstrating clone representation of the library in each of its available formats, along with functional validation of the viral particles at the level of both viral integration and functional RNA expression.

Our ready-to-use Mission TRC3 LentiORFs allow for stable integration, enrichment of cells, and long-term gene expression in difficult-to-transfect cell lines utilizing our best-in-class lentiviral manufacturing.  This collection provides researchers with unique tools to gain insights into gene function by modulating gene and protein expression.
 

 What Will You Learn?

  • What the MISSION® TRC3 Human LentiORF Collection is and how it complements other screening methods
  • How to design a gain-of-function screen using LentiORFs
  • Ways to validate integration and expression of ORFs in your transduced cells

 

Speaker Bio
Stacey Ward, PhD
Senior R&D Scientist, Cell Design Studio
MilliporeSigma
Stacey received her Ph.D. in Microbiology and Immunology from the State University of New York Upstate Medical University in Syracuse, NY, where she studied the dysregulation of cell cycle protein expression and activity by varicella zoster virus. She completed a postdoctoral appointment at the University of Connecticut Health Science Center investigating herpes simplex virus DNA replication before transitioning to an NIH-funded postdoctoral fellowship in brain cancer biology at Washington University in St. Louis School of Medicine dissecting the role of sonic hedgehog signaling in medulloblastoma. Stacey joined MilliporeSigma in 2016 and has been integrating the use of next generation sequencing in cell line engineering and library deconvolution and expression.