SigmaSpin Removes Interfering Reaction Components and Eliminates Dye Blobs

Sequencing reaction was purified using a SigmaSpin 96-well plate. pFLAG™ MAC plasmid (E 5644) was sequenced using BigDye™ Terminator v 3.0 chemistry (20 µl total reaction volume; BigDye premix diluted 1:1 with SeqSaver™ (S3938)). Data was generated on an ABI Prism. 3700 DNA Analyzer with POP-6™ polymer and 10X CE Buffer (B4930). Phred 20 > 600.

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