Sensitive QRT–PCR using the SYBR Green QRT–PCR Kit

Quantitative SYBR Green RT–PCR was performed in duplicate on human total RNA from cell line HeLa–S3. Total RNA was diluted 10–fold in subsequent capillaries with concentrations of 500 ng to 5 pg. Primers specific for a ß–actin 187 bp RT–PCR product were used for amplification.

Close Window