Proligo® Phosphoramidites and Reagents for Oligonucleotide Synthesis

The Proligo® reagents portfolio offers a full range of phosphoramidites, solid supports and liquid reagents for oligonucleotide synthesis that include the following features:

  • High quality standard and specialized products for the complete workflow
  • Flexible and customizable production and packaging options

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synthesis cycle

Sigma-Aldrich has a complete product portfolio for solid phase oligonucleotide synthesis ranging from controlled pore glass and phosphoramidites to the whole family of synthesis reagents including custom formulations.

Start of Synthesis: Controlled Pore Glass (bulk or prepack)

Detritylation: Deblock

Activation: Activators

Coupling:

Capping: Cap A and Cap B

Oxidation: Oxidizers

Solvents and Labware
View our complete range of Acetonitrile, Solvents and Labware for every phase of Oligonucleotide Synthesis.

Improve yields and reduce unwanted side reactions with high quality solvents and reagents used in cleavage and deprotection including:

Solvents and Labware
View our complete range of Acetonitrile, Solvents and Labware for every phase of Oligonucleotide Synthesis.

desalting

Sigma-Aldrich can help with the removal of organic salts through a variety of methods including:

Size Exclusion - see more info on General Electric NAP Columns

Reverse Phase Catridge Purification - see more information on Solid Phase Extraction products

Extraction: Butanol

Solvents and Labware
View our complete range of Acetonitrile, Solvents and Labware for every phase of Oligonucleotide Synthesis.

purification

Access a wide variety of products and tools for use in determining product composition and purity profile.

Solvents and Labware
View our complete range of Acetonitrile, Solvents and Labware for every phase of Oligonucleotide Synthesis.

synthesis cycle

Oligonucleotides are manufactured on custom designed high throughput synthesis or commercially available platforms. Sigma-Aldrich formulates and packages ready-to-use reagents for all commercial synthesizers.

Platform:

Solvents and Labware
View our complete range of Acetonitrile, Solvents and Labware for every phase of Oligonucleotide Synthesis.

Synthesis Supports

Controlled Pore Glass (CPG) hasproven to be the most acceptedsupports for the chemical synthesisof DNA and RNA. Features includehigh surface area, efficient masstransfer and chemical inertness.

Controlled Pore Glass

Detritylation & Activation

Coupling takes place once theDMT groups are removed throughthe detritylation mechanismutilizing TCA.

Detritylation Products:

Deblock

Activator solutions are mixed withsolutions of phosphoramiditesduring the extension step. Theactivator reacts with the amiditegroup to form a highly reactiveintermediate. The intermediatethen forms an internucleotidebond with the detritylated 5′hydroxylgroup of the growing oligonucleotidechain.

Activation Product:

Activators

Coupling

Phosphoramidite monomers are added through the coupling mechanism utilizing the following products:

Coupling Products:

DNA Amidites
  (Standard and Fast Deprotection)
RNA Amidites
  (Standard and Fast Deprotection)
Linkers
Dyes and Label

Capping & Oxidation

Stabilization of the reactions andefficiency of the yield is achievedthrough oxidation and capping.

Capping Products:

Cap A
Cap B

Oxidization Products:

Oxidizers

 Capabilities and Services

 

Quality Assurance
Dedicated process technology group and in-process controls that will adapt to evolving diagnostic and pharmaceutical requirements.

 

 

Contact Us
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State-of-the-Art Production
Complete packaging services from routine to custom and from milligrams to kilograms at our state-of-the-Art Manufacturing facility in Hamburg.