HyStem™ Cell Culture

HyStem™-C Cell Culture Scaffold Kits and 96-well plate

Customizable, well characterized and consistent, HyStem-C hydrogel cell culture scaffolds provide a complex, three dimensional environment in which cells are able to proliferate, much as they would in‑vivo.
Each distinct cell type in a multicellular organism has its own unique microenvironment. HyStem-C offers the flexibility to tailor the environments of cultured cells to mimic their in vivo counterparts and optimize proliferation and differentiation.
As a synthetic matrix, HyStem-C offers greater control of the cellular environment than ambiguous extracted ECM alternatives. All components are well characterized and consistently formulated. HyStem-C contains Hyaluronic acid (synthetic), Extralink (synthetic), Gelin-S (porcine), and Water.
Biologically accurate
Rich in hyaluronic acid and denatured collagen, the HyStem-C hydrogel closely mimics the complex three dimensional cellular environments found in life, providing an optimal environment for culturing your cells.
Recommended For...
HyStem-C is recommended for researchers who require a large number of generalized cell attachment sites for their stem cell culture(s).
With HyStem-C, researchers can tailor the incorporation of ECM proteins and growth factors as well as the elasticity of the hydrogel and cell incorporation (encapsulations or top plating) to create the specific 3D environment required by their cells.
Cell Types
The HyStem Hydrogel Kits are optimal for culturing stem cells whose natural environment is rich in hyaluronic acid (HA). HyStem-C has been successfully used to expand human embryonic stem cells, human mesenchymal stem cells, neural progenitor cells and hepatic progenitor cells.
Cell Encapsulation
The HyStem platform accommodates cell encapsulations (true 3D) as well as plating on top of the hydrogel for pseudo-3D growth.
Cell Attachment
HyStem-C enables the attachment of a wide variety of cell types through Gelin-S (thiolated gelatin).
Growth Factor Incorporation
Researchers may customize with their own growth factors; growth factors will likely require replenishment
  • HyStem (H2416)
  • Gelin-S (G3673)
  • Extralink 2 (E6659)
  • Water, degassed (W3894)
Ordering Information and Formats:
Trial Kit: (HYSC010) for 2.5 ml of HyStem Hydrogel
Standard Kit: (HYSC020) for 7.5 ml of HyStem Hydrogel
96-well plate: (H2666)
The HyStem-C Trial Kit enables researchers to evaluate the HyStem-C technology in their own hands and confirm that HyStem-C is the right choice for their research.
HyStem-C is recommended for in vitro experimentation.
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