Neural Stem Cell Culture

Neural stem cells (NSCs) are self-renewing, multipotent cells that generate the basic cell types of the nervous system. NSCs primarily differentiate into neurons, astrocytes, and oligodendrocytes, depending on environmental cues. The use of neural stem cells in research and medicine is becoming increasingly widespread. The discovery that neurons, astrocytes, and oligodendrocytes arise from neural stem cells located in specific regions of the brain, such as SVZ and hippocampus, reveals the potential of using NSCs to treat central nervous system diseases, including Parkinson’s and Alzheimer’s disease. More recently, induced pluripotent stem cells (iPSCs) have been proposed as an alternative efficient method of generating neural stem cells.

MilliporeSigma offers a broad range of tools and technologies to culture neural stem cells including proprietary human and rodent NSC lines from various sources, optimized serum-free expansion and differentiation media, media supplements and a broad range or NSC related antibodies.

 

 Neural Stem Cell Lines

Human Neural Stem Cell Lines

MilliporeSigma offers a variety of proprietary human neural stem cell lines derived from various sources including iPSC, fetal and embryonic stem cells. All NSC lines come with optimized serum-free expansion media and reagents to ensure proper expansion.

 

ReNcell™ Neural Progenitors

ENStem-A™ Neural Progenitors

XCell Sciences Neural Cells

OPC Cells

Neural Stem Cells

Neural Stem Cells

Neurons, Astrocytes, Dopaminergic Progenitors

Oligodendrocyte Progenitors

Fetal Tissue Derived

Human ES Cell Derived

Human iPSC Derived

Human ES Cell Derived

Immortalized

Non-Immortalized

Non-Immortalized

Non-Immortalized

ReNcell® VM: derived from the ventral mesencephalon brain region

ReNcell® CX: derived from the cortical brain region.

H9 human embryonic stem cells derived NSCs that proliferate as an adherent monolayer.

Gene-edited isogenic knockout neural cells that mimic loss of function of genes associated with various CNS disorders.

Easily generate enriched populations of mature human oligodendrocytes in just 2-3 weeks.

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Product Highlight: ReNcell™ Human Neural Progenitor Cell Lines

ReNcell™ Human Neural Stem Cells are robust immortalized neural stem cell lines derived from developing human brains. ReNcell® VM and CX cells are generated from the ventral mesencephalon and cortical regions of the brain, respectively, and transduced with the myc transcription factor. Both cell lines offer phenotype and genotype stability, in addition to the multipotential neuronal differentiation capacity, over long-term culture. ReNcell lines are have been published in nearly 1000 publications.

Figure 1. ReNcell CX cells (SCC007) are grown as monolayers (A) and express NSC markers, Nestin (MAB5326) and Sox-2 (AB5603). ReNcell CX cells are able to differentiate (C, D) into neurons expressing βIII-tubulin (MAB1637) and glial cells expressing GFAP (AB5804).

Protocol Guide: 3D Neural Stem Cell Models of Alzheimer’s Disease Protocol

Application Note: ReNcell® Human Neural Progenitors: Renewable and Consistent Human Neural Stem Cell Lines that Differentiate into Functional Neurons

Application Note: Derivation of Functional Oligodendrocyte Progenitor Cells (OPCs) from Human Neural Stem Cell Lines

Mouse Neural Stem Cell Lines

Rat Neural Stem Cell Lines

 

 Neural Stem Cell Media and Supplements

Neural Stem Cell Expansion Media

Serum-free neural stem cell medium and supplements optimized for the maximum growth and survival of primary and iPSC-derived neural stem cells in culture. Historically, N-2 (SCM012) and B-27 (SCM013) supplements have been added to Neurobasal media, along with cytokines bFGF and EGF, to create serum-free NSC expansion media. However, these supplements exhibit large performance variability. New NS21 supplement (SCM081) supports a higher and more consistent neuronal culture demonstrated by enhanced neural morphological characteristics, formation of synapses, and postsynaptic responses.

NS21: re-defined and modified supplement B27 for neuronal cultures. J Neurosci Methods. 2008 Jun 30;171(2):239-47.
 

Description Part Number
ReNcell NSC Maintenance Media SCM005
ENStem-A Neural Expansion Medium SCM004
Neural Stem Cell Basal Medium SCM003
Rat Neural Stem Cell Basal Medium (250 ml) R812-250
Rat Neural Stem Cell Growth Medium (250ml) R813-250
Neural Stem Cell Freezing Medium (1X) SCM014
ReNcell Neural Stem Cell Freezing Medium SCC007
NDiff Neuro-2 Medium Supplement (200x) SCM012
NDiff Neuro-27 Medium Supplement (100x) SCM013
N21 Medium Supplement (50X) SCM081

 

Stemline® Neural Stem Cell Expansion Medium

  • Consistent robust expansion of human neural stem cells in both neurosphere and monolayer cultures.
  • Serum-free and animal origin-free formulation
  • Produced in a GMP state-of-the-art facility with an available Device Master File (DMF).

Order Stemline NSC Expanion Media

 

 

Neural Stem Cell Differentiation Media

Human ES/iPS Neural Induction Media

The discovery that somatic cells could be converted into induced pluripotent stem (iPS) cells with the expression of four transcription factors has created an exciting new area of stem cell biology research. MilliporeSigma offers ready-to-use differentiation media that incorporate supplements, small molecule inhibitors, and growth factors to easily produce neural progenitors and specific neural and glial subtypes from human iPS cells.

Application Note: Robust Differentiation of Human iPSCs into Lineage-Specific Neuronal and Glial Cells Utilizing Dual-SMAD Inhibition

 

Figure 2. Workflow showing all steps in iPS cell generation and subsequent differentiation to neural lineages. In as few as four steps, adult fibroblasts can be converted to neural lineages using media formulations for modulating cell fate. Along with iPS cell-generating reprogramming technologies (STEMCCA™ and Simplicon™ kits), EMD Millipore now offers media to generate different neural and glial subtypes from iPS cells for “disease-in-a-dish” researchers.

 

Part Number

Item Description

SCM110

Human ES/iPS Neural Induction Medium

SCM111

Human ES/iPS Neuronal Differentiation Medium

SCR603

Human ES/iPS Neurogenesis Kit

SCR135

Human Dopaminergic Neurogenesis Kit

 

 

 Neural Stem Cell Characterization

Neural Stem Cell Antibodies

Popular Neural Stem Cell and Lineage Specific Antibodies
 

Progenitor Markers:

Nestin

Sox-2

Pax-6

N-Cadherin 

Musashi

CD133

Vimentin

NeuroD

 

Neuronal Markers:

β-tubulin

MAP2

NeuN

VGlut

Tyrosine Hydroxylase

Synapsin

Tau    

DCX ChAT

Glial Markers:

GFAP

Olig1

Olig2

MBP

Gal-C

MOG

S100-beta

ALDH1L1

GLT-1

Browse all of our Neuroscience Antibodies Here

The Human Neural Stem Cell Characterization Kit contains three molecular markers, Nestin, Sox-2 and Musashi that are frequently used to identify neural stem/progenitor cells along with more differentiated lineage markers including b-III-tubulin for neurons, GFAP for astrocytes and O1 for oligodendrocytes.

AXIS: Axon Investigation System

The AXIS microfluidic device can be used to study neurite outgrowth in living cells. Each device is composed of two wells and an interconnected channel, separated by a set of microgrooves. The hydrostatic pressure formed by volume differential between chambers induces fluidic isolation of the solution on the low volume side of the device. The microfluidic design of an AXIS device allows for development and maintenance of a fluidic gradient of chemoattractants, toxins or other molecules of interest, facilitating controlled exposure and differentiation of axons.

Figure 3. E18 rat hippocampal cells were loaded and cultured for five days in an AX450 device. Cells were fixed and triple staining was performed using DAPI (S7113 in blue), anti-Map2 (AB15452 in green), and anti-βIII Tubulin (MAB1637 in red) as shown in panel A. βIII Tubulin is an axonal marker, Map2 is a dendritic marker, and DAPI is a stain for cell nuclei. Panel B is the same image as in panel A except with on overlay of the white light image to verify the location of the channels and microgrooves. Note that the cells are contained in the lower channel where they were loaded and only the axons extend through the microgrooves and into the opposite channel.

Cell Culture