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HPLC Analysis of 16 Triazine and Phenyl Urea Compounds on Ascentis® Express RP-Amide, Modified Gradient

HPLC Analysis of 16 Triazine and Phenyl Urea Compounds on Ascentis® Express RP-Amide, Modified Gradient

Conditions

column Ascentis Express RP-Amide, 15 cm x 3 mm I.D., 2.7 µm particles (53919-U)
column temp. 35 °C
mobile phase [A] water; [B] acetonitrile
gradient 10 to 25% B in 30 min; 25 to 60% B in 15 min; held at 60% B for 5 min
flow rate 0.4 mL/min
sample 20 mg/L each (except terbuthylazin desethyl and terbuthylazine 60 mg/L) in 60:40, water: acetonitrile
injection 5 µL
detector UV, 240 nm

Description

Analysis Note This application demonstrates the suitability of Ascentis Express RP-Amide for the efficient separation of 16 triazine and phenyl urea compounds. Lower column
temperature can improve resolution of the critical pair.
Categories Analytical Chromatography, Pesticides & Herbicides
Featured Industry Agriculture
Chemicals and Industrial Polymers
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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