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HPLC Analysis of a Monoclonal Antibody on Zenix® SEC-150, 30 cm X 4.6 mm I.D., 3 μm particles after Proteolysis with IdeS Protease

HPLC Analysis of a Monoclonal Antibody on Zenix® SEC-150, 30 cm X 4.6 mm I.D., 3 μm particles after Proteolysis with IdeS Protease

Conditions

column Zenix® SEC-150, 30 cm X 4.6 mm I.D., 3 µm particles (Z777018)
column temp. ambient
mobile phase 0.15 M potassium phosphate monobasic, pH 7.0 with potassium hydroxide
flow rate 0.35 mL/min
pressure 1450 psi (100 bar)
sample digested SO57 mAb, ~0.5 g/L
injection 1 µL
detector UV, 215 nm

Description

Analysis Note Proteolysis of antibodies can be useful for simplifying further analysis. This application demonstrates separation of the two fragments, F(ab′)2 and scFc, after digestion with IdeS protease
Categories Analytical Chromatography, Proteins, Peptides
Featured Industry Life Science and Biopharma
Legal Information Zenix is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
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